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The map label for this gene is miaA

Identifier: 15606346

GI number: 15606346

Start: 738025

End: 738942

Strand: Direct

Name: miaA

Synonym: aq_1067

Alternate gene names: 15606346

Gene position: 738025-738942 (Clockwise)

Preceding gene: 15606344

Following gene: 15606349

Centisome position: 47.57

GC content: 39.98

Gene sequence:

>918_bases
ATGAAGATACTCGTAATCGGAGGACCTACAGCGATAGGAAAGTCAGACCTCGCATGTTGTGTAGCACAAAAGCTGAACGG
TGAAATCATAAGTGCGGATAGTATGGCGGTTTACAAGTTCATGGACATAGGAACAGCAAAACCTCTTGAGTGTATGAAAA
AAGTTCCCCACCACCTCATAGACGTAGTAGAGCCGGGCGGATACTTTGACGCAAAGATTTTTGAGGAAATGGCAAAAGAA
AAGATAGAAGAGATAAAAAGAAAGGGAAAAGTCCCCATAGTCGTGGGAGGGACTTATCTCTACATTCAGGCACTCCTTTA
CGGGATAGACGAGACTCCAAAACCCGACTGGAATTTAAGAAATAAACTCTACGAAATAGCCAGAAAAAAGGGAAACGATT
ACCTTTATGAAAAGTTAAAAGCCATAGACCCAAAATACGCAAAAAAAATACACAAAAACGATTTAAGGAGAATAGTGAGA
GCTCTGGAAGTTTTCATAAACACGGGAAAACCCTTTTCCTCCTTCCACTCTTGGAACAAACCTAAAATGGACTTTGTAGG
GATATACCTGAAGAGAAGTCCCGAAAGTTTATACAAAAGAATAGAAAACAGAGTTTACGACATGGTAAAGGACGGACTCC
TTGAAGAGGTTAAAAAGTTATTAGAAATGGGATACGAGAACTTCCTGACTTCCGGGCAGGCTATAGATTACAAGGAATTT
GTTCCCTGTGCAAAAGGGGAGAAGAGTTTAGAAGAGTGTATAAAAGAGGCGATAAAAAACACAAAGAAACAGGCAAAGAG
GCAGATAAGATGGTTCAGAAAACAAGGCTGGCACGAAATAGACCTTGACAAACTGAGTATTGAAGAAGCGTGCGAAGAAG
TTGTGAGGATTTATAAAGATGCAAATAAAACTAAGTGA

Upstream 100 bases:

>100_bases
AGCTCTGAAAAAGCTCCTCCCTATCCTACCGAATCCGTTTATCCCTACTTTTATAGCCATAATTCTTAATTATACTCGTG
GAACTATTTTATATAAACTT

Downstream 100 bases:

>100_bases
ACACTTTTGGTATAGATGGGATAAGAGAAAGGATTTTATGCTGAAGGAAGGCATAACACCAGAGAAGATAATAGAATTTG
TATTAAATCCCGATTTAACT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MKILVIGGPTAIGKSDLACCVAQKLNGEIISADSMAVYKFMDIGTAKPLECMKKVPHHLIDVVEPGGYFDAKIFEEMAKE
KIEEIKRKGKVPIVVGGTYLYIQALLYGIDETPKPDWNLRNKLYEIARKKGNDYLYEKLKAIDPKYAKKIHKNDLRRIVR
ALEVFINTGKPFSSFHSWNKPKMDFVGIYLKRSPESLYKRIENRVYDMVKDGLLEEVKKLLEMGYENFLTSGQAIDYKEF
VPCAKGEKSLEECIKEAIKNTKKQAKRQIRWFRKQGWHEIDLDKLSIEEACEEVVRIYKDANKTK

Sequences:

>Translated_305_residues
MKILVIGGPTAIGKSDLACCVAQKLNGEIISADSMAVYKFMDIGTAKPLECMKKVPHHLIDVVEPGGYFDAKIFEEMAKE
KIEEIKRKGKVPIVVGGTYLYIQALLYGIDETPKPDWNLRNKLYEIARKKGNDYLYEKLKAIDPKYAKKIHKNDLRRIVR
ALEVFINTGKPFSSFHSWNKPKMDFVGIYLKRSPESLYKRIENRVYDMVKDGLLEEVKKLLEMGYENFLTSGQAIDYKEF
VPCAKGEKSLEECIKEAIKNTKKQAKRQIRWFRKQGWHEIDLDKLSIEEACEEVVRIYKDANKTK
>Mature_305_residues
MKILVIGGPTAIGKSDLACCVAQKLNGEIISADSMAVYKFMDIGTAKPLECMKKVPHHLIDVVEPGGYFDAKIFEEMAKE
KIEEIKRKGKVPIVVGGTYLYIQALLYGIDETPKPDWNLRNKLYEIARKKGNDYLYEKLKAIDPKYAKKIHKNDLRRIVR
ALEVFINTGKPFSSFHSWNKPKMDFVGIYLKRSPESLYKRIENRVYDMVKDGLLEEVKKLLEMGYENFLTSGQAIDYKEF
VPCAKGEKSLEECIKEAIKNTKKQAKRQIRWFRKQGWHEIDLDKLSIEEACEEVVRIYKDANKTK

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=315, Percent_Identity=31.7460317460317, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI1790613, Length=302, Percent_Identity=35.0993377483444, Blast_Score=179, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI25144712, Length=267, Percent_Identity=32.2097378277154, Blast_Score=111, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6324848, Length=288, Percent_Identity=38.1944444444444, Blast_Score=188, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24649830, Length=283, Percent_Identity=33.9222614840989, Blast_Score=135, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_AQUAE (O67162)

Other databases:

- EMBL:   AE000657
- PIR:   G70391
- RefSeq:   NP_213725.1
- ProteinModelPortal:   O67162
- SMR:   O67162
- GeneID:   1193796
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_1067
- NMPDR:   fig|224324.1.peg.740
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- PhylomeDB:   O67162
- ProtClustDB:   PRK00091
- BioCyc:   AAEO224324:AQ_1067-MONOMER
- BRENDA:   2.5.1.8
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 35225; Mature: 35225

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVIGGPTAIGKSDLACCVAQKLNGEIISADSMAVYKFMDIGTAKPLECMKKVPHHLI
CEEEEECCCCCCCCHHHHHHHHHHHCCCEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHH
DVVEPGGYFDAKIFEEMAKEKIEEIKRKGKVPIVVGGTYLYIQALLYGIDETPKPDWNLR
HHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHCCCCCCCCCHHH
NKLYEIARKKGNDYLYEKLKAIDPKYAKKIHKNDLRRIVRALEVFINTGKPFSSFHSWNK
HHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCC
PKMDFVGIYLKRSPESLYKRIENRVYDMVKDGLLEEVKKLLEMGYENFLTSGQAIDYKEF
CCHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCHHHH
VPCAKGEKSLEECIKEAIKNTKKQAKRQIRWFRKQGWHEIDLDKLSIEEACEEVVRIYKD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
ANKTK
CCCCC
>Mature Secondary Structure
MKILVIGGPTAIGKSDLACCVAQKLNGEIISADSMAVYKFMDIGTAKPLECMKKVPHHLI
CEEEEECCCCCCCCHHHHHHHHHHHCCCEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHH
DVVEPGGYFDAKIFEEMAKEKIEEIKRKGKVPIVVGGTYLYIQALLYGIDETPKPDWNLR
HHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHCCCCCCCCCHHH
NKLYEIARKKGNDYLYEKLKAIDPKYAKKIHKNDLRRIVRALEVFINTGKPFSSFHSWNK
HHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCC
PKMDFVGIYLKRSPESLYKRIENRVYDMVKDGLLEEVKKLLEMGYENFLTSGQAIDYKEF
CCHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCHHHH
VPCAKGEKSLEECIKEAIKNTKKQAKRQIRWFRKQGWHEIDLDKLSIEEACEEVVRIYKD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
ANKTK
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320