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The map label for this gene is mbhS3 [H]

Identifier: 15606171

GI number: 15606171

Start: 552695

End: 553549

Strand: Direct

Name: mbhS3 [H]

Synonym: aq_802

Alternate gene names: 15606171

Gene position: 552695-553549 (Clockwise)

Preceding gene: 15606170

Following gene: 15606172

Centisome position: 35.63

GC content: 47.37

Gene sequence:

>855_bases
ATGAAACTACTCTGGCTTCAACGACTTTCATGTTGTGGTAACACTCACTCCCTCCTATCCTCGGAAGAGGCAAATCTCCT
GTTTGAAGAGTTTGAAGTTCTTTTCCACCCCTCTTTTTCCACGGAAAGTGAGGAAGATGTCATAGAAAGAGTCTTAAAAG
GAGGAAAGCTAGATCTTCTGGTAGTTGAAGGAGCTGTAAAAAGGGACGATGAGGTTATTAAAAATCTCTGCGGTATTTCC
GATTACGTTGTAGCCGTGGGAAATTGTGCGGTCTACGGAAATATTCCCGCACTTTCGGAGGACAGCGTCTTCGGACTTCA
ATACAGGTTCAAAGAAAAGGGTGGTATTCTGGGAAAAGATTTTGAGAGTAAAAAAGGGTATCCGGTCATAAACCTGTCCG
GCTGTCCTGCAAATCCCGAGTGGATAGTCGGCACACTCTTGAGGATAAAGTGGAAAGGTAAACTGGACTTGGACGAGCTG
GGGCGCCCAAAAGAGTACTACTCTTCCCTTACCCACTGGGGTTGTTCCAGAAACGAGTACTTCGAGTGGAAGGTGGAGAC
GGAAAAACTCGGCTCTCCTAAAGGTTGTCTCTTTTACCACTACGGTTGCAGGGGACCAATGACCCACTCCACTTGTAACG
TTCACCTGTGGAACGGTGTGAACTCAAAAACGAGGGCGGGCACCCCCTGCTTTGGATGTACGGAGTACGACTTTCCAAGA
GTTAACCTGTGGGAAACAAAGCTTTACGCTGGCATACCGGCGGAACTACCCTTGGGAGTTTCCAAGAGGGGATACATAAT
GATATCCGGGGTGGCAAAGATGTTTGCTCCCGAAAGGTTGAAAGATGAAGATTGA

Upstream 100 bases:

>100_bases
TAATTTATTAATTGGCACGGGGTGTAGCGCAGGTGGTAGCGCGCTGGCATGGGGGGCCAGAGGTCGCCGGTTCGAGTCCG
GTCACCCCGACCACTTTCCT

Downstream 100 bases:

>100_bases
AAAACTGGTTCTCACCCGCGTTGAAGGGGAGGCCTCTTTAAACCTCGTCTGGGAAAGGGGTGTTATAAAAGACGCAAAAA
TTAGTTTTTACTCTACAAGA

Product: hydrogenase small subunit

Products: NA

Alternate protein names: NiFe hydrogenlyase small chain [H]

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MKLLWLQRLSCCGNTHSLLSSEEANLLFEEFEVLFHPSFSTESEEDVIERVLKGGKLDLLVVEGAVKRDDEVIKNLCGIS
DYVVAVGNCAVYGNIPALSEDSVFGLQYRFKEKGGILGKDFESKKGYPVINLSGCPANPEWIVGTLLRIKWKGKLDLDEL
GRPKEYYSSLTHWGCSRNEYFEWKVETEKLGSPKGCLFYHYGCRGPMTHSTCNVHLWNGVNSKTRAGTPCFGCTEYDFPR
VNLWETKLYAGIPAELPLGVSKRGYIMISGVAKMFAPERLKDED

Sequences:

>Translated_284_residues
MKLLWLQRLSCCGNTHSLLSSEEANLLFEEFEVLFHPSFSTESEEDVIERVLKGGKLDLLVVEGAVKRDDEVIKNLCGIS
DYVVAVGNCAVYGNIPALSEDSVFGLQYRFKEKGGILGKDFESKKGYPVINLSGCPANPEWIVGTLLRIKWKGKLDLDEL
GRPKEYYSSLTHWGCSRNEYFEWKVETEKLGSPKGCLFYHYGCRGPMTHSTCNVHLWNGVNSKTRAGTPCFGCTEYDFPR
VNLWETKLYAGIPAELPLGVSKRGYIMISGVAKMFAPERLKDED
>Mature_284_residues
MKLLWLQRLSCCGNTHSLLSSEEANLLFEEFEVLFHPSFSTESEEDVIERVLKGGKLDLLVVEGAVKRDDEVIKNLCGIS
DYVVAVGNCAVYGNIPALSEDSVFGLQYRFKEKGGILGKDFESKKGYPVINLSGCPANPEWIVGTLLRIKWKGKLDLDEL
GRPKEYYSSLTHWGCSRNEYFEWKVETEKLGSPKGCLFYHYGCRGPMTHSTCNVHLWNGVNSKTRAGTPCFGCTEYDFPR
VNLWETKLYAGIPAELPLGVSKRGYIMISGVAKMFAPERLKDED

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd2 Is Involved In Hydrogen Uptake. [C]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789371, Length=259, Percent_Identity=27.027027027027, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1787206, Length=263, Percent_Identity=23.1939163498099, Blast_Score=67, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.2.1 [H]

Molecular weight: Translated: 31930; Mature: 31930

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLWLQRLSCCGNTHSLLSSEEANLLFEEFEVLFHPSFSTESEEDVIERVLKGGKLDLL
CCCHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEEEE
VVEGAVKRDDEVIKNLCGISDYVVAVGNCAVYGNIPALSEDSVFGLQYRFKEKGGILGKD
EECCCCCCCHHHHHHHHCCCCEEEEECCEEEECCCCCCCCCCEEEEEEEECCCCCCCCCC
FESKKGYPVINLSGCPANPEWIVGTLLRIKWKGKLDLDELGRPKEYYSSLTHWGCSRNEY
CCCCCCCEEEEECCCCCCCCCEEEEEEEEEECCCCCHHHCCCCHHHHHHHHHCCCCCCCE
FEWKVETEKLGSPKGCLFYHYGCRGPMTHSTCNVHLWNGVNSKTRAGTPCFGCTEYDFPR
EEEEEEHHHCCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCC
VNLWETKLYAGIPAELPLGVSKRGYIMISGVAKMFAPERLKDED
CEEEEEEEEECCCCCCCCCCCCCCEEEEECHHHHHCCCCCCCCC
>Mature Secondary Structure
MKLLWLQRLSCCGNTHSLLSSEEANLLFEEFEVLFHPSFSTESEEDVIERVLKGGKLDLL
CCCHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEEEE
VVEGAVKRDDEVIKNLCGISDYVVAVGNCAVYGNIPALSEDSVFGLQYRFKEKGGILGKD
EECCCCCCCHHHHHHHHCCCCEEEEECCEEEECCCCCCCCCCEEEEEEEECCCCCCCCCC
FESKKGYPVINLSGCPANPEWIVGTLLRIKWKGKLDLDELGRPKEYYSSLTHWGCSRNEY
CCCCCCCEEEEECCCCCCCCCEEEEEEEEEECCCCCHHHCCCCHHHHHHHHHCCCCCCCE
FEWKVETEKLGSPKGCLFYHYGCRGPMTHSTCNVHLWNGVNSKTRAGTPCFGCTEYDFPR
EEEEEEHHHCCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCC
VNLWETKLYAGIPAELPLGVSKRGYIMISGVAKMFAPERLKDED
CEEEEEEEEECCCCCCCCCCCCCCEEEEECHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3322743; 2651421; 3322275; 3134950; 7854413 [H]