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The map label for this gene is mbhL3 [H]

Identifier: 15606172

GI number: 15606172

Start: 553539

End: 554789

Strand: Direct

Name: mbhL3 [H]

Synonym: aq_804

Alternate gene names: 15606172

Gene position: 553539-554789 (Clockwise)

Preceding gene: 15606171

Following gene: 15606178

Centisome position: 35.68

GC content: 43.8

Gene sequence:

>1251_bases
ATGAAGATTGAAAAACTGGTTCTCACCCGCGTTGAAGGGGAGGCCTCTTTAAACCTCGTCTGGGAAAGGGGTGTTATAAA
AGACGCAAAAATTAGTTTTTACTCTACAAGAGGCATAGAAAAGGTTCTAAGGGAAAGACCCTTTATGGATGCTTTAGTTA
TAAACCCGAGGATTTGTGGCATATGCGGGCACGCACATCTCATAGCCACGGTGAGGGCAATAGAGAACGCTATAGGTATT
AAAGAAATACCTGAAAAGGCTAAGATTACTAGACTCGTAACCCAGATAACGGAAATGGTTCAAAACCACGTAAAGTGGTT
TTACCTCTTCGTAATGCCGGACTTCCTGAAATTTAAGGAGAGTTTATCCCAGTTCGAACCTTTTAAAGGAGAAAGGTGGA
AAAGAGCCGTTCAATTTTCCTCTCAGATAGTGAAGATTATCGCCCTCTTCGGAGGACAGTGGCCCCATTCCTCCTACGCC
GTTCCCGGAGGAATTACATCAAACTTCTCGGAAAGGGAAGTCCTGAAAGCTTTAAACCTTGTAAAAGAGGGAAAGACATT
TTTTGAAAAGAACGTTTCCGAAGATTTAGAGCTTTTCCTGAACCTGTGCGAAGAATTTAACCTTTTGAGTATAGGGAAGG
CTTACAATAGGTTCTTGAGCGGAGGCGGTTTAGCTTACTGTTTTAACCCGTCCTACAAAAAGGGAAAGGGAAAAGTGTGT
AAGTTCAACGTTAGATATGTGCAGGAACTCGAAGCAGCCGATTACTCAAAAGCTAATCCCGTAAGGTACAAAGGCTTACC
TTACGAGACAGGGCCCCTCGCCCGAGAACTGATCTCTAAAAATCCCCTTGTCCTGAAACTTTACAAAAATTACGGAGACA
GTTACGCGGTGAGAGTTGCGGCAAGACTTGCAGAGATTAAAGATCTACTCGAAATCCTTGAAAAGCTTTTGAAAGAATTA
ATGAATCACTTGGAAGAACCTTCGTGTTTATGGGAGGATCGCAGTGATGAAAGCGGTGAAGGGTTCGGAGTTGTAGAGGC
GGCGCGGGGAACACTCATACACAGGGTCGTAATAGAGAAAGGGAAAATAAAGGATTACAAAGTGATAACTCCCTCCCAGT
GGAACCTAGGACCGCGGTGTAAAAAGTACTTGGGAGTGGCTGAAAAAGCTATCGTAGGACTGGACAGCGAACTAAAGGCT
CAAATGGTTCTAAGAAGTTTTGACCTCTGTTCCGTATGTACCACGAAGTGA

Upstream 100 bases:

>100_bases
TACGCTGGCATACCGGCGGAACTACCCTTGGGAGTTTCCAAGAGGGGATACATAATGATATCCGGGGTGGCAAAGATGTT
TGCTCCCGAAAGGTTGAAAG

Downstream 100 bases:

>100_bases
TTACTTAATCACCGTCAGGAATATACTGAACTCACCATTTTTAACCTTGTACTTCGTAATCGATAACTTACTCCTGAACT
GTTTGGGAACCTTCTGGTAA

Product: hydrogenase large subunit

Products: NA

Alternate protein names: NiFe hydrogenlyase large chain [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MKIEKLVLTRVEGEASLNLVWERGVIKDAKISFYSTRGIEKVLRERPFMDALVINPRICGICGHAHLIATVRAIENAIGI
KEIPEKAKITRLVTQITEMVQNHVKWFYLFVMPDFLKFKESLSQFEPFKGERWKRAVQFSSQIVKIIALFGGQWPHSSYA
VPGGITSNFSEREVLKALNLVKEGKTFFEKNVSEDLELFLNLCEEFNLLSIGKAYNRFLSGGGLAYCFNPSYKKGKGKVC
KFNVRYVQELEAADYSKANPVRYKGLPYETGPLARELISKNPLVLKLYKNYGDSYAVRVAARLAEIKDLLEILEKLLKEL
MNHLEEPSCLWEDRSDESGEGFGVVEAARGTLIHRVVIEKGKIKDYKVITPSQWNLGPRCKKYLGVAEKAIVGLDSELKA
QMVLRSFDLCSVCTTK

Sequences:

>Translated_416_residues
MKIEKLVLTRVEGEASLNLVWERGVIKDAKISFYSTRGIEKVLRERPFMDALVINPRICGICGHAHLIATVRAIENAIGI
KEIPEKAKITRLVTQITEMVQNHVKWFYLFVMPDFLKFKESLSQFEPFKGERWKRAVQFSSQIVKIIALFGGQWPHSSYA
VPGGITSNFSEREVLKALNLVKEGKTFFEKNVSEDLELFLNLCEEFNLLSIGKAYNRFLSGGGLAYCFNPSYKKGKGKVC
KFNVRYVQELEAADYSKANPVRYKGLPYETGPLARELISKNPLVLKLYKNYGDSYAVRVAARLAEIKDLLEILEKLLKEL
MNHLEEPSCLWEDRSDESGEGFGVVEAARGTLIHRVVIEKGKIKDYKVITPSQWNLGPRCKKYLGVAEKAIVGLDSELKA
QMVLRSFDLCSVCTTK
>Mature_416_residues
MKIEKLVLTRVEGEASLNLVWERGVIKDAKISFYSTRGIEKVLRERPFMDALVINPRICGICGHAHLIATVRAIENAIGI
KEIPEKAKITRLVTQITEMVQNHVKWFYLFVMPDFLKFKESLSQFEPFKGERWKRAVQFSSQIVKIIALFGGQWPHSSYA
VPGGITSNFSEREVLKALNLVKEGKTFFEKNVSEDLELFLNLCEEFNLLSIGKAYNRFLSGGGLAYCFNPSYKKGKGKVC
KFNVRYVQELEAADYSKANPVRYKGLPYETGPLARELISKNPLVLKLYKNYGDSYAVRVAARLAEIKDLLEILEKLLKEL
MNHLEEPSCLWEDRSDESGEGFGVVEAARGTLIHRVVIEKGKIKDYKVITPSQWNLGPRCKKYLGVAEKAIVGLDSELKA
QMVLRSFDLCSVCTTK

Specific function: Catalyzes the reversible oxidoreduction of molecular hydrogen, in conjunction with a specific electron acceptor, cytochrome c3 [H]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=236, Percent_Identity=27.1186440677966, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1789368, Length=130, Percent_Identity=30.7692307692308, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.2.1 [H]

Molecular weight: Translated: 47191; Mature: 47191

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: PS00507 NI_HGENASE_L_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIEKLVLTRVEGEASLNLVWERGVIKDAKISFYSTRGIEKVLRERPFMDALVINPRICG
CCCHHHHHEEECCCCCEEEEEECCCCCCCCEEEHHHHHHHHHHHHCCCCCEEEECCCEEE
ICGHAHLIATVRAIENAIGIKEIPEKAKITRLVTQITEMVQNHVKWFYLFVMPDFLKFKE
ECCHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHH
SLSQFEPFKGERWKRAVQFSSQIVKIIALFGGQWPHSSYAVPGGITSNFSEREVLKALNL
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
VKEGKTFFEKNVSEDLELFLNLCEEFNLLSIGKAYNRFLSGGGLAYCFNPSYKKGKGKVC
HHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEE
KFNVRYVQELEAADYSKANPVRYKGLPYETGPLARELISKNPLVLKLYKNYGDSYAVRVA
EEEHHHHHHHHHCCCCCCCCCEECCCCCCCCHHHHHHHCCCCEEEEEECCCCCHHHHHHH
ARLAEIKDLLEILEKLLKELMNHLEEPSCLWEDRSDESGEGFGVVEAARGTLIHRVVIEK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
GKIKDYKVITPSQWNLGPRCKKYLGVAEKAIVGLDSELKAQMVLRSFDLCSVCTTK
CCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHCCCC
>Mature Secondary Structure
MKIEKLVLTRVEGEASLNLVWERGVIKDAKISFYSTRGIEKVLRERPFMDALVINPRICG
CCCHHHHHEEECCCCCEEEEEECCCCCCCCEEEHHHHHHHHHHHHCCCCCEEEECCCEEE
ICGHAHLIATVRAIENAIGIKEIPEKAKITRLVTQITEMVQNHVKWFYLFVMPDFLKFKE
ECCHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHH
SLSQFEPFKGERWKRAVQFSSQIVKIIALFGGQWPHSSYAVPGGITSNFSEREVLKALNL
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
VKEGKTFFEKNVSEDLELFLNLCEEFNLLSIGKAYNRFLSGGGLAYCFNPSYKKGKGKVC
HHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEE
KFNVRYVQELEAADYSKANPVRYKGLPYETGPLARELISKNPLVLKLYKNYGDSYAVRVA
EEEHHHHHHHHHCCCCCCCCCEECCCCCCCCHHHHHHHCCCCEEEEEECCCCCHHHHHHH
ARLAEIKDLLEILEKLLKELMNHLEEPSCLWEDRSDESGEGFGVVEAARGTLIHRVVIEK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
GKIKDYKVITPSQWNLGPRCKKYLGVAEKAIVGLDSELKAQMVLRSFDLCSVCTTK
CCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2269874; 9438867 [H]