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Identifier: 11500015

GI number: 11500015

Start: 293385

End: 294242

Strand: Reverse

Name: Not Available

Synonym: AF0323a

Alternate gene names: 11500015

Gene position: 294242-293385 (Counterclockwise)

Preceding gene: 11497937

Following gene: 11500016

Centisome position: 13.51

GC content: 48.37

Gene sequence:

>858_bases
ATGAAGGTAGCGGTAATAGGAGCCAAAGGCCAGCTTGGAACGGACCTTGTTGACGTTCTTAAGGAGGAGTTCACAGTTTA
TCCGCTTACTCATGAGGATGTTGATGTTGCAGTGTCTGAAAGCCTTGAAGTGCTGAGAGACATCCACCCCGACGTTATCA
TAAACACCGCTGCCTATGTCAGAGTTGATGATGCGGAGAATCATGCGGAAAAGGCCTTTGCGGTCAACGCCATCGGGGCG
CTTAATGTGGCCAGAATCTCTTCGGAAATTGATGCGGTTAACATTTACATCAGTACAGACTACGTTTTCGATGGTGAGAA
GGGAGAGCCCTATGTCGAGACAGATAAGCCAAATCCTATAAACGTTTACGGAGCGAGCAAGTATGCCGGAGAAATTCTCA
CGAAAAACTACTCGGAAAAACACTACGTGATCAGGGTTGCAAGTCTATACGGGAAGGCCGGGGCGAGCGGAAAGGGCGGT
AACTTCGTAAACTGGGTGGTGGATATGGCGAGAAAGGGGCAGGAGCTGAGGATTGTTGCGGACCAGTTTATGAGCCCCAC
CTATACCAAGGACGTGGCCCTCGCTGTTAGAGAGTTTCTCAAACTCCGCCCCGACTACGGAATTTACCACATGGTAAACG
ACGGATACTGCTCGTGGTTTGAGTTTACGAAGGCAATATTCGAAATTCTCGACTGGGATGTGGAAATTCAGCCAATAAAG
TCGAGCGAGCTTAACAGATTGGCGAGAAGGCCAGCTTTCTCTGCCCTGCAAAACTACAAACTCGAAAGAATTGGCATTAA
GATGAGGCACTGGAGGGAAGCTTTAAGAGATTATTTAGCTGAGAAGGGTTTGGTTTGA

Upstream 100 bases:

>100_bases
AGGAGGACTGTTGAGTGGTACCTGCAAAACGAGTGGTGGTGGAGGCCTCTGGCTGATGAGAGGATTTTGCATCCCACGCC
ATGGAAGCTGAGATGGTAGA

Downstream 100 bases:

>100_bases
TTGGTGGTAGTATGCTGCCAGGTATCGTTGTTAAACCGCTCAAAACCTTCGCAGACGAGAGGGGTTTCTTCACGGAGATA
ATGCGAAGAGACTGGGATGT

Product: dTDP-4-dehydrorhamnose reductas

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MKVAVIGAKGQLGTDLVDVLKEEFTVYPLTHEDVDVAVSESLEVLRDIHPDVIINTAAYVRVDDAENHAEKAFAVNAIGA
LNVARISSEIDAVNIYISTDYVFDGEKGEPYVETDKPNPINVYGASKYAGEILTKNYSEKHYVIRVASLYGKAGASGKGG
NFVNWVVDMARKGQELRIVADQFMSPTYTKDVALAVREFLKLRPDYGIYHMVNDGYCSWFEFTKAIFEILDWDVEIQPIK
SSELNRLARRPAFSALQNYKLERIGIKMRHWREALRDYLAEKGLV

Sequences:

>Translated_285_residues
MKVAVIGAKGQLGTDLVDVLKEEFTVYPLTHEDVDVAVSESLEVLRDIHPDVIINTAAYVRVDDAENHAEKAFAVNAIGA
LNVARISSEIDAVNIYISTDYVFDGEKGEPYVETDKPNPINVYGASKYAGEILTKNYSEKHYVIRVASLYGKAGASGKGG
NFVNWVVDMARKGQELRIVADQFMSPTYTKDVALAVREFLKLRPDYGIYHMVNDGYCSWFEFTKAIFEILDWDVEIQPIK
SSELNRLARRPAFSALQNYKLERIGIKMRHWREALRDYLAEKGLV
>Mature_285_residues
MKVAVIGAKGQLGTDLVDVLKEEFTVYPLTHEDVDVAVSESLEVLRDIHPDVIINTAAYVRVDDAENHAEKAFAVNAIGA
LNVARISSEIDAVNIYISTDYVFDGEKGEPYVETDKPNPINVYGASKYAGEILTKNYSEKHYVIRVASLYGKAGASGKGG
NFVNWVVDMARKGQELRIVADQFMSPTYTKDVALAVREFLKLRPDYGIYHMVNDGYCSWFEFTKAIFEILDWDVEIQPIK
SSELNRLARRPAFSALQNYKLERIGIKMRHWREALRDYLAEKGLV

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=285, Percent_Identity=30.1754385964912, Blast_Score=95, Evalue=6e-20,
Organism=Homo sapiens, GI11034825, Length=285, Percent_Identity=30.1754385964912, Blast_Score=95, Evalue=6e-20,
Organism=Escherichia coli, GI1788352, Length=300, Percent_Identity=31.6666666666667, Blast_Score=141, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 32108; Mature: 32108

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVAVIGAKGQLGTDLVDVLKEEFTVYPLTHEDVDVAVSESLEVLRDIHPDVIINTAAYV
CEEEEEECCCCCCHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEEECEEEE
RVDDAENHAEKAFAVNAIGALNVARISSEIDAVNIYISTDYVFDGEKGEPYVETDKPNPI
EEECCCCHHHHHHEEHHHHHHHHHHHHCCCEEEEEEEEEEEEEECCCCCCCEECCCCCEE
NVYGASKYAGEILTKNYSEKHYVIRVASLYGKAGASGKGGNFVNWVVDMARKGQELRIVA
EEEECCHHHHHHHHCCCCCCEEEEEEEHHHCCCCCCCCCCCHHHHHHHHHHCCCEEEEEH
DQFMSPTYTKDVALAVREFLKLRPDYGIYHMVNDGYCSWFEFTKAIFEILDWDVEIQPIK
HHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEECC
SSELNRLARRPAFSALQNYKLERIGIKMRHWREALRDYLAEKGLV
CHHHHHHHHCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKVAVIGAKGQLGTDLVDVLKEEFTVYPLTHEDVDVAVSESLEVLRDIHPDVIINTAAYV
CEEEEEECCCCCCHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEEECEEEE
RVDDAENHAEKAFAVNAIGALNVARISSEIDAVNIYISTDYVFDGEKGEPYVETDKPNPI
EEECCCCHHHHHHEEHHHHHHHHHHHHCCCEEEEEEEEEEEEEECCCCCCCEECCCCCEE
NVYGASKYAGEILTKNYSEKHYVIRVASLYGKAGASGKGGNFVNWVVDMARKGQELRIVA
EEEECCHHHHHHHHCCCCCCEEEEEEEHHHCCCCCCCCCCCHHHHHHHHHHCCCEEEEEH
DQFMSPTYTKDVALAVREFLKLRPDYGIYHMVNDGYCSWFEFTKAIFEILDWDVEIQPIK
HHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEECC
SSELNRLARRPAFSALQNYKLERIGIKMRHWREALRDYLAEKGLV
CHHHHHHHHCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]