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The map label for this gene is rfbB

Identifier: 11497937

GI number: 11497937

Start: 294244

End: 295242

Strand: Reverse

Name: rfbB

Synonym: AF0324

Alternate gene names: 11497937

Gene position: 295242-294244 (Counterclockwise)

Preceding gene: 11497938

Following gene: 11500015

Centisome position: 13.55

GC content: 47.95

Gene sequence:

>999_bases
ATGAGAATTCTTGTTACGGGAGGAATGGGGTTCATCGGCTCCAACTTCGTTAGGTACGCTCTTGCTCACCACCAGGTAGA
GATTCTGAACCTCGATGCTCTGAAGTACGGCTCCAATCCGGAAAATCTGAAAGACTTGGCAGAGGATGAGAGATACAGCT
TCATCAAAGGGGATATAAACGACTATGAACTCGTCTCTGATCTGATTAAGAAGGTAGATGCGGTGGTGAACTTCGCTGCA
GAGAGCCATGTGGACAGGAGTATATCCAGTCCCTATGCCTTCATCGAGAGTAACTTTCTTGGAGTGTATACGATTCTCGA
AGCAGTGAGAAAGTGCAATCCTGAGGTAAGATTGGTGCACGTCAGCACGGATGAGGTTTACGGGGACATTGAAAAAGGCT
CCTTTACGGAAAAGGACGGGTTGATGCCTTCCTCACCCTACTCAGCAAGTAAAGCTGCTGCAGACATGCTGGTCCTCGGC
TATGCAAGGACTTACAACCTAAACGCATCCATTACAAGGTGCACGAACAACTATGGGCCCTATCAATTCCCGGAAAAACT
GATTCCGAAGACCATAATAAGGGCAAAAATGAATCTGAGCGTCCCAATCTACGGAACCGGAATGAACGTGAGGGACTGGA
TTTACGTCGAGGACCACTGTAGAGCAGTCTGGGAGGTTATGGAGAAGGGGGAGAGAAGAGAAGTTTACAACATTTCAAGC
GGAGAGGAAAGAACGAACCTTGAGATTGTAAAAACGATTCTGGAAATCATGGGGAAAGACGAGAGCCTGATTGAGTATGT
TGAGGATAGACCAGGACATGACGTTCGCTACTCCCTCGACTCGTGGAAGATAATGAGAGAGCTGAAATGGAGGCCAAAGA
TTAGCTTTGAAGAAGGGATAAGGAGGACTGTTGAGTGGTACCTGCAAAACGAGTGGTGGTGGAGGCCTCTGGCTGATGAG
AGGATTTTGCATCCCACGCCATGGAAGCTGAGATGGTAG

Upstream 100 bases:

>100_bases
GTCTCATTGGGAGGAAAGTCAGAATCAGAAAGAACGACAGAAAGAGGTTCCTAAGATTCACCATTGGGGATTACTCCGAG
ATCCTGCTTTGAGGTGGAAG

Downstream 100 bases:

>100_bases
AATGAAGGTAGCGGTAATAGGAGCCAAAGGCCAGCTTGGAACGGACCTTGTTGACGTTCTTAAGGAGGAGTTCACAGTTT
ATCCGCTTACTCATGAGGAT

Product: dTDP-glucose 4,6-dehydratase (rfbB)

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MRILVTGGMGFIGSNFVRYALAHHQVEILNLDALKYGSNPENLKDLAEDERYSFIKGDINDYELVSDLIKKVDAVVNFAA
ESHVDRSISSPYAFIESNFLGVYTILEAVRKCNPEVRLVHVSTDEVYGDIEKGSFTEKDGLMPSSPYSASKAAADMLVLG
YARTYNLNASITRCTNNYGPYQFPEKLIPKTIIRAKMNLSVPIYGTGMNVRDWIYVEDHCRAVWEVMEKGERREVYNISS
GEERTNLEIVKTILEIMGKDESLIEYVEDRPGHDVRYSLDSWKIMRELKWRPKISFEEGIRRTVEWYLQNEWWWRPLADE
RILHPTPWKLRW

Sequences:

>Translated_332_residues
MRILVTGGMGFIGSNFVRYALAHHQVEILNLDALKYGSNPENLKDLAEDERYSFIKGDINDYELVSDLIKKVDAVVNFAA
ESHVDRSISSPYAFIESNFLGVYTILEAVRKCNPEVRLVHVSTDEVYGDIEKGSFTEKDGLMPSSPYSASKAAADMLVLG
YARTYNLNASITRCTNNYGPYQFPEKLIPKTIIRAKMNLSVPIYGTGMNVRDWIYVEDHCRAVWEVMEKGERREVYNISS
GEERTNLEIVKTILEIMGKDESLIEYVEDRPGHDVRYSLDSWKIMRELKWRPKISFEEGIRRTVEWYLQNEWWWRPLADE
RILHPTPWKLRW
>Mature_332_residues
MRILVTGGMGFIGSNFVRYALAHHQVEILNLDALKYGSNPENLKDLAEDERYSFIKGDINDYELVSDLIKKVDAVVNFAA
ESHVDRSISSPYAFIESNFLGVYTILEAVRKCNPEVRLVHVSTDEVYGDIEKGSFTEKDGLMPSSPYSASKAAADMLVLG
YARTYNLNASITRCTNNYGPYQFPEKLIPKTIIRAKMNLSVPIYGTGMNVRDWIYVEDHCRAVWEVMEKGERREVYNISS
GEERTNLEIVKTILEIMGKDESLIEYVEDRPGHDVRYSLDSWKIMRELKWRPKISFEEGIRRTVEWYLQNEWWWRPLADE
RILHPTPWKLRW

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=335, Percent_Identity=37.3134328358209, Blast_Score=229, Evalue=2e-60,
Organism=Homo sapiens, GI42516563, Length=338, Percent_Identity=23.9644970414201, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI4504031, Length=276, Percent_Identity=25.7246376811594, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI48994969, Length=341, Percent_Identity=47.2140762463343, Blast_Score=313, Evalue=1e-86,
Organism=Escherichia coli, GI1788353, Length=340, Percent_Identity=48.2352941176471, Blast_Score=310, Evalue=8e-86,
Organism=Escherichia coli, GI1786974, Length=341, Percent_Identity=26.6862170087977, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788366, Length=345, Percent_Identity=23.768115942029, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788365, Length=250, Percent_Identity=25.6, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1788589, Length=344, Percent_Identity=22.9651162790698, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17568069, Length=322, Percent_Identity=34.1614906832298, Blast_Score=194, Evalue=7e-50,
Organism=Caenorhabditis elegans, GI115532424, Length=322, Percent_Identity=34.1614906832298, Blast_Score=174, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI17539532, Length=314, Percent_Identity=25.4777070063694, Blast_Score=98, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI133901786, Length=283, Percent_Identity=24.7349823321555, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17539422, Length=283, Percent_Identity=24.7349823321555, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI133901788, Length=283, Percent_Identity=24.7349823321555, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI133901790, Length=283, Percent_Identity=24.7349823321555, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17539424, Length=283, Percent_Identity=24.7349823321555, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17507723, Length=280, Percent_Identity=23.9285714285714, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71982035, Length=344, Percent_Identity=25, Blast_Score=71, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI71982038, Length=346, Percent_Identity=24.8554913294798, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=349, Percent_Identity=25.214899713467, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI21356223, Length=319, Percent_Identity=25.705329153605, Blast_Score=98, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24158427, Length=275, Percent_Identity=27.2727272727273, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19923002, Length=340, Percent_Identity=26.1764705882353, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46

Molecular weight: Translated: 38536; Mature: 38536

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILVTGGMGFIGSNFVRYALAHHQVEILNLDALKYGSNPENLKDLAEDERYSFIKGDIN
CEEEEECCCCHHHHHHHHHHHHHCEEEEEEEEHEECCCCHHHHHHHHHHHHHEEEECCCC
DYELVSDLIKKVDAVVNFAAESHVDRSISSPYAFIESNFLGVYTILEAVRKCNPEVRLVH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHCCCCEEEEE
VSTDEVYGDIEKGSFTEKDGLMPSSPYSASKAAADMLVLGYARTYNLNASITRCTNNYGP
EEHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHEEEEEEEECCCCEEEEECCCCCC
YQFPEKLIPKTIIRAKMNLSVPIYGTGMNVRDWIYVEDHCRAVWEVMEKGERREVYNISS
CCCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEHHHHHHHHHHHHCCCCCEEEECCC
GEERTNLEIVKTILEIMGKDESLIEYVEDRPGHDVRYSLDSWKIMRELKWRPKISFEEGI
CCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEHHHHHHHHHHCCCCCCCHHHHH
RRTVEWYLQNEWWWRPLADERILHPTPWKLRW
HHHHHHHHCCCCEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRILVTGGMGFIGSNFVRYALAHHQVEILNLDALKYGSNPENLKDLAEDERYSFIKGDIN
CEEEEECCCCHHHHHHHHHHHHHCEEEEEEEEHEECCCCHHHHHHHHHHHHHEEEECCCC
DYELVSDLIKKVDAVVNFAAESHVDRSISSPYAFIESNFLGVYTILEAVRKCNPEVRLVH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHCCCCEEEEE
VSTDEVYGDIEKGSFTEKDGLMPSSPYSASKAAADMLVLGYARTYNLNASITRCTNNYGP
EEHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHEEEEEEEECCCCEEEEECCCCCC
YQFPEKLIPKTIIRAKMNLSVPIYGTGMNVRDWIYVEDHCRAVWEVMEKGERREVYNISS
CCCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEHHHHHHHHHHHHCCCCCEEEECCC
GEERTNLEIVKTILEIMGKDESLIEYVEDRPGHDVRYSLDSWKIMRELKWRPKISFEEGI
CCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEHHHHHHHHHHCCCCCCCHHHHH
RRTVEWYLQNEWWWRPLADERILHPTPWKLRW
HHHHHHHHCCCCEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose

Specific reaction: dTDP-glucose = dTDP-4-dehydro-6-deoxy-D-glucose + H2O

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]