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The map label for this gene is trpE

Identifier: 11499196

GI number: 11499196

Start: 1437891

End: 1439126

Strand: Reverse

Name: trpE

Synonym: AF1603

Alternate gene names: 11499196

Gene position: 1439126-1437891 (Counterclockwise)

Preceding gene: 11499197

Following gene: 11499195

Centisome position: 66.06

GC content: 52.59

Gene sequence:

>1236_bases
ATGCAAAAGCACGAGTATGTAAACCCGGTGAAGCTCTACTCAGCCATAAGAGACGAGAAATTTCCGTTCATTCTCGAATC
CGCGGAGAAGAGCGGCAGGGCGAGATACACATACATATCCTTCAACCCCCTCTACACGGTAAGGGTCGGCAGCAGAACCA
GGGTTGATGGGGAGGTTATTTCAAAAATCTCGGACCCCTTCGATGCGCTGAATGAGATTCACGTTAAGGGATTGCTTGTC
GGCTACGTTGCCTACGAGGCGGTGAAGAACTATATTGGCAAGAAGCCCCAAACCCCGTCGGTTTTTGGCTGCTACGATGG
TTACTTCGTTTACGACCACTACCTGAGAAAGCTCTTCAGCGTAAACGTTGAGAACGCGGATAAGATTGTCGAGAGGGCGA
AGAGGGTAGAGGTTGAGCAAGTGAGGGGGAATTCGGAGGTCCTGAGGGCCGGAAGCAGGGAGAAGTTTGAGAAGATGGTG
GAGAGAGGGAAGGAGCAGATTTTCGAGGGCGAGGTTTACCAGATTGTGCTTTCAAGGGAGTATGTTGTTGATACGGATTT
GAGCCCTTTCCAGATGTATCTGAATTTGAGGGAGACGAATCCGAGCCCCTACATGTTCCTGCTTGAGTTCGACAGGGCCC
TGATCGGCTCAAGCCCGGAGACGATGGGAAGGGTTGAGGGAAACAGCTTCATAATCAACCCGATTGCCGGAACCGCCAGG
AGGGAGGCGGGAAGGGAGAAAGAGATTGCTGAAAAGCTCCTCAGCGATGAGAAGGAGAGGGCTGAGCACGTCATGCTTGT
TGACCTAGCCAGAAACGACGTGAGGAAGGTTTGCAGAGCAGGGAGCGTTAGAGTTTCGAGGTTCATGGAGGTTGTGGAGT
ATCCGAGCGTTCTGCACATCGAAAGCGAGGTTGTTGGAGAGCTGAAGGCGGGAGTGACGCACTTCGACGCTATGAAAGCC
ACCTTTCCCGCAGGAACCGTTACCGGAGCGCCGAAACTGAGGGCGATTGAGCTCATAGACGAAATTGAGGGGGATTGCAG
AGGGGTTTACGCTGGTGCTGTTGGCTACTTCAGCGAGAATGTTTCTGATCTGGCCATTGCAATCAGGATGATTGAGTTCG
ACGGAAAGGCGAGGATAAGGGCAGGTGCGGGGATAGTTGCGGACTCCGTTCCTGAAAGGGAGTTTTTCGAGACGGAAAAC
AAGATAGCGAGGGTTTTGAGGGCGGTTGGGCTATGA

Upstream 100 bases:

>100_bases
CACTGTTTGCCCTCGGCTATGAGGACCTGAAGGAGAACGTTGAGATTTTTGAGGAAAAGGTTCAGAGCGGGGAGCTTGCG
AGAAAGCTGGAGGAAATCGC

Downstream 100 bases:

>100_bases
TAGTCGTTGTTGACTGCAAGGACTCCTTCGTTTACAATCTCGTGGAGTACATCTCTCTGTTCGACAAGGTAAGGGTTGTT
GAGAAGGAGAGTGCTGGATT

Product: anthranilate synthase component I

Products: NA

Alternate protein names: Anthranilate synthase component I

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MQKHEYVNPVKLYSAIRDEKFPFILESAEKSGRARYTYISFNPLYTVRVGSRTRVDGEVISKISDPFDALNEIHVKGLLV
GYVAYEAVKNYIGKKPQTPSVFGCYDGYFVYDHYLRKLFSVNVENADKIVERAKRVEVEQVRGNSEVLRAGSREKFEKMV
ERGKEQIFEGEVYQIVLSREYVVDTDLSPFQMYLNLRETNPSPYMFLLEFDRALIGSSPETMGRVEGNSFIINPIAGTAR
REAGREKEIAEKLLSDEKERAEHVMLVDLARNDVRKVCRAGSVRVSRFMEVVEYPSVLHIESEVVGELKAGVTHFDAMKA
TFPAGTVTGAPKLRAIELIDEIEGDCRGVYAGAVGYFSENVSDLAIAIRMIEFDGKARIRAGAGIVADSVPEREFFETEN
KIARVLRAVGL

Sequences:

>Translated_411_residues
MQKHEYVNPVKLYSAIRDEKFPFILESAEKSGRARYTYISFNPLYTVRVGSRTRVDGEVISKISDPFDALNEIHVKGLLV
GYVAYEAVKNYIGKKPQTPSVFGCYDGYFVYDHYLRKLFSVNVENADKIVERAKRVEVEQVRGNSEVLRAGSREKFEKMV
ERGKEQIFEGEVYQIVLSREYVVDTDLSPFQMYLNLRETNPSPYMFLLEFDRALIGSSPETMGRVEGNSFIINPIAGTAR
REAGREKEIAEKLLSDEKERAEHVMLVDLARNDVRKVCRAGSVRVSRFMEVVEYPSVLHIESEVVGELKAGVTHFDAMKA
TFPAGTVTGAPKLRAIELIDEIEGDCRGVYAGAVGYFSENVSDLAIAIRMIEFDGKARIRAGAGIVADSVPEREFFETEN
KIARVLRAVGL
>Mature_411_residues
MQKHEYVNPVKLYSAIRDEKFPFILESAEKSGRARYTYISFNPLYTVRVGSRTRVDGEVISKISDPFDALNEIHVKGLLV
GYVAYEAVKNYIGKKPQTPSVFGCYDGYFVYDHYLRKLFSVNVENADKIVERAKRVEVEQVRGNSEVLRAGSREKFEKMV
ERGKEQIFEGEVYQIVLSREYVVDTDLSPFQMYLNLRETNPSPYMFLLEFDRALIGSSPETMGRVEGNSFIINPIAGTAR
REAGREKEIAEKLLSDEKERAEHVMLVDLARNDVRKVCRAGSVRVSRFMEVVEYPSVLHIESEVVGELKAGVTHFDAMKA
TFPAGTVTGAPKLRAIELIDEIEGDCRGVYAGAVGYFSENVSDLAIAIRMIEFDGKARIRAGAGIVADSVPEREFFETEN
KIARVLRAVGL

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family

Homologues:

Organism=Escherichia coli, GI1787518, Length=273, Percent_Identity=42.4908424908425, Blast_Score=184, Evalue=8e-48,
Organism=Escherichia coli, GI1788114, Length=316, Percent_Identity=32.9113924050633, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1786809, Length=264, Percent_Identity=29.1666666666667, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87082077, Length=260, Percent_Identity=28.0769230769231, Blast_Score=86, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6320935, Length=432, Percent_Identity=33.5648148148148, Blast_Score=217, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6324361, Length=398, Percent_Identity=25.6281407035176, Blast_Score=109, Evalue=8e-25,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPE_ARCFU (O28669)

Other databases:

- EMBL:   AE000782
- PIR:   B69450
- RefSeq:   NP_070432.1
- ProteinModelPortal:   O28669
- SMR:   O28669
- GeneID:   1484829
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF1603
- NMPDR:   fig|224325.1.peg.1593
- TIGR:   AF_1603
- HOGENOM:   HBG507440
- OMA:   NDVRKVC
- ProtClustDB:   PRK13572
- BioCyc:   AFUL224325:AF_1603-MONOMER
- BRENDA:   4.1.3.27
- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR010116
- InterPro:   IPR015890
- Gene3D:   G3DSA:3.60.120.10
- PANTHER:   PTHR11236
- PRINTS:   PR00095
- TIGRFAMs:   TIGR01820

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind; SSF56322 TRPE_1_chor_bd

EC number: =4.1.3.27

Molecular weight: Translated: 46346; Mature: 46346

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKHEYVNPVKLYSAIRDEKFPFILESAEKSGRARYTYISFNPLYTVRVGSRTRVDGEVI
CCCCCCCCHHHHHHHHHCCCCCCEEECCCCCCCEEEEEEEECCEEEEEECCCCCCCHHHH
SKISDPFDALNEIHVKGLLVGYVAYEAVKNYIGKKPQTPSVFGCYDGYFVYDHYLRKLFS
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHH
VNVENADKIVERAKRVEVEQVRGNSEVLRAGSREKFEKMVERGKEQIFEGEVYQIVLSRE
CCCCCHHHHHHHHHHCHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEECC
YVVDTDLSPFQMYLNLRETNPSPYMFLLEFDRALIGSSPETMGRVEGNSFIINPIAGTAR
EEEECCCCCEEEEEEEECCCCCCEEEEEEHHHHHHCCCCHHHCEECCCCEEEECCCCHHH
REAGREKEIAEKLLSDEKERAEHVMLVDLARNDVRKVCRAGSVRVSRFMEVVEYPSVLHI
HHCCCHHHHHHHHHCCHHHHHHEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEE
ESEVVGELKAGVTHFDAMKATFPAGTVTGAPKLRAIELIDEIEGDCRGVYAGAVGYFSEN
HHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEHHHHHHHHCC
VSDLAIAIRMIEFDGKARIRAGAGIVADSVPEREFFETENKIARVLRAVGL
HHHHHHEEEEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQKHEYVNPVKLYSAIRDEKFPFILESAEKSGRARYTYISFNPLYTVRVGSRTRVDGEVI
CCCCCCCCHHHHHHHHHCCCCCCEEECCCCCCCEEEEEEEECCEEEEEECCCCCCCHHHH
SKISDPFDALNEIHVKGLLVGYVAYEAVKNYIGKKPQTPSVFGCYDGYFVYDHYLRKLFS
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHH
VNVENADKIVERAKRVEVEQVRGNSEVLRAGSREKFEKMVERGKEQIFEGEVYQIVLSRE
CCCCCHHHHHHHHHHCHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEECC
YVVDTDLSPFQMYLNLRETNPSPYMFLLEFDRALIGSSPETMGRVEGNSFIINPIAGTAR
EEEECCCCCEEEEEEEECCCCCCEEEEEEHHHHHHCCCCHHHCEECCCCEEEECCCCHHH
REAGREKEIAEKLLSDEKERAEHVMLVDLARNDVRKVCRAGSVRVSRFMEVVEYPSVLHI
HHCCCHHHHHHHHHCCHHHHHHEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEE
ESEVVGELKAGVTHFDAMKATFPAGTVTGAPKLRAIELIDEIEGDCRGVYAGAVGYFSEN
HHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEHHHHHHHHCC
VSDLAIAIRMIEFDGKARIRAGAGIVADSVPEREFFETENKIARVLRAVGL
HHHHHHEEEEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475