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The map label for this gene is trpD

Identifier: 11499197

GI number: 11499197

Start: 1439105

End: 1440745

Strand: Reverse

Name: trpD

Synonym: AF1604

Alternate gene names: 11499197

Gene position: 1440745-1439105 (Counterclockwise)

Preceding gene: 11499198

Following gene: 11499196

Centisome position: 66.14

GC content: 51.98

Gene sequence:

>1641_bases
GTGATGGACTTTGGGTTTGTTGATTCGTTGAAGGGAGCAAGCAAAAGAGGTAAGAACGCAGTCATTGCCGAGGTTAAGGT
TCGATCCCCCATACACGGCGATTTGCTTAGAGGGAGGAGGATTGAGGACATCTTGAGGGCCTACGAAAAAGCTGGTGCAG
CGGCGATTTCGTACATAACAGCAGAGCAGTTCAGCGGAAATTTCGAGACGCTGAAGAAGATTGTTGGGCTTACCGATCTG
CCCGTTCTGAGGAAGGACTTCATAAGGGGCAGAAAGGAGGTTGAAAGAACTGCTGAGGTTGAGGCTGCAGCTTTGCTGCT
CATAGCGAGGCATCTGAAGGAGAGAACTGCGGAAATGGTTGATTTTTGCTTCGAGCACGGCATCGAGCCTCTGGTTGAGG
TTCATCACGCTGAGGATTTGGTTTACGCCGAAAACGCAAGGGCTGTGCTGATAAACAACCGCGACATTGACCGCATGGAG
AGGGATGGGGGAAGCATAGATGTCACAGCAAAAATTGCCGAAAAAATCAGGGCTTTCAAGGTTAGCGGGAGCGGAATTGG
CAGCGTGGAAGATTTGCTCTTTGTTCTGCAGTATGTGGATGCAGCCCTCATAGGGACCGCCTTCATGATGGCTGAGAACA
CCGAAGAATTTGTCCAGACAGTTTGTGGAGGCGAGAAGATGATAGAGGATGTTTTGAGGGGGCTGGACTTCGATAAGGCC
TACGAGCTCGCAAAAACGCTGCCCGAACTTGATGAAATCAAGATTGCAGCAGTTCTCGCAGCTCTGGAGGCTAAGGGCTA
CGGTGCAGAGGTAATCGCCGGGTTCGCGAAGGGGGTTGCAGAGAAGTCCAAAATCGAGATCGGCAAGGTTATGGACACCT
GCGGGACGGGAGGAGACAAAACGAGCTCGATAAACGTAAGCACGGCTGTTGCTATTGCGCTGTCAACAGTCCATCCCGTT
GCAAAGCACGGAAACAGAGCAGTCAGCTCCAAAAGCGGTTCGGCTGACGTTCTGGAAGCTCTCGGTGTGAGAATTGAGAT
GGATGAAGAGAGGGCGAGGAAAATGATTGCCGAAACCAACTTTGCCTTCCTCTTCGCCCCCCTTTACCACAAAAGCTTTG
CAAGGGTTGCCGCTGTGAGAAGAAATCTGGGGATACGGACGATTTTCAACGTCACTGGGCCTTTAACCAACCCCGCAAGG
CCTGAGGTGCAAATTGTCGGTGTGGCGAGTGAAATTCTGCTGGTTGAGGTTGCAAAGGCTATGTCGCTGCTCGGCAGAAG
AGCGGTCGTGGTTTATGGCAGCGGTATGGACGAGGTCAACCCTAACAGCTCAACTGACATCGCCGTTGTTAATGGCGGAG
TTGAGAGGCTTAAACTTGAGCCGGAGGATTTCGGCATCGAGAGGTGCAGAGTTTTGCCATGCAGCAGCAGCGGGGAGTCG
GCTGAGAGGATAAGGGCTGTGTTCAGCGGAAAGGGGCTGAAGGAGGACAGAAGGCTCATTGCCATAAACTTTGCAACCGC
ACTGTTTGCCCTCGGCTATGAGGACCTGAAGGAGAACGTTGAGATTTTTGAGGAAAAGGTTCAGAGCGGGGAGCTTGCGA
GAAAGCTGGAGGAAATCGCATGCAAAAGCACGAGTATGTAA

Upstream 100 bases:

>100_bases
TCTTATCAGAAAGTTTAGGTGACTTTTACCTCTTTTTTGCCATACTATAGCAAAAAGCTTAAATACACTTCGCGGGATTA
ATCCCACATGGTGGGAGCAG

Downstream 100 bases:

>100_bases
ACCCGGTGAAGCTCTACTCAGCCATAAGAGACGAGAAATTTCCGTTCATTCTCGAATCCGCGGAGAAGAGCGGCAGGGCG
AGATACACATACATATCCTT

Product: anthranilate synthase component II (trpD)

Products: NA

Alternate protein names: Indole-3-glycerol phosphate synthase; IGPS; Anthranilate phosphoribosyltransferase

Number of amino acids: Translated: 546; Mature: 546

Protein sequence:

>546_residues
MMDFGFVDSLKGASKRGKNAVIAEVKVRSPIHGDLLRGRRIEDILRAYEKAGAAAISYITAEQFSGNFETLKKIVGLTDL
PVLRKDFIRGRKEVERTAEVEAAALLLIARHLKERTAEMVDFCFEHGIEPLVEVHHAEDLVYAENARAVLINNRDIDRME
RDGGSIDVTAKIAEKIRAFKVSGSGIGSVEDLLFVLQYVDAALIGTAFMMAENTEEFVQTVCGGEKMIEDVLRGLDFDKA
YELAKTLPELDEIKIAAVLAALEAKGYGAEVIAGFAKGVAEKSKIEIGKVMDTCGTGGDKTSSINVSTAVAIALSTVHPV
AKHGNRAVSSKSGSADVLEALGVRIEMDEERARKMIAETNFAFLFAPLYHKSFARVAAVRRNLGIRTIFNVTGPLTNPAR
PEVQIVGVASEILLVEVAKAMSLLGRRAVVVYGSGMDEVNPNSSTDIAVVNGGVERLKLEPEDFGIERCRVLPCSSSGES
AERIRAVFSGKGLKEDRRLIAINFATALFALGYEDLKENVEIFEEKVQSGELARKLEEIACKSTSM

Sequences:

>Translated_546_residues
MMDFGFVDSLKGASKRGKNAVIAEVKVRSPIHGDLLRGRRIEDILRAYEKAGAAAISYITAEQFSGNFETLKKIVGLTDL
PVLRKDFIRGRKEVERTAEVEAAALLLIARHLKERTAEMVDFCFEHGIEPLVEVHHAEDLVYAENARAVLINNRDIDRME
RDGGSIDVTAKIAEKIRAFKVSGSGIGSVEDLLFVLQYVDAALIGTAFMMAENTEEFVQTVCGGEKMIEDVLRGLDFDKA
YELAKTLPELDEIKIAAVLAALEAKGYGAEVIAGFAKGVAEKSKIEIGKVMDTCGTGGDKTSSINVSTAVAIALSTVHPV
AKHGNRAVSSKSGSADVLEALGVRIEMDEERARKMIAETNFAFLFAPLYHKSFARVAAVRRNLGIRTIFNVTGPLTNPAR
PEVQIVGVASEILLVEVAKAMSLLGRRAVVVYGSGMDEVNPNSSTDIAVVNGGVERLKLEPEDFGIERCRVLPCSSSGES
AERIRAVFSGKGLKEDRRLIAINFATALFALGYEDLKENVEIFEEKVQSGELARKLEEIACKSTSM
>Mature_546_residues
MMDFGFVDSLKGASKRGKNAVIAEVKVRSPIHGDLLRGRRIEDILRAYEKAGAAAISYITAEQFSGNFETLKKIVGLTDL
PVLRKDFIRGRKEVERTAEVEAAALLLIARHLKERTAEMVDFCFEHGIEPLVEVHHAEDLVYAENARAVLINNRDIDRME
RDGGSIDVTAKIAEKIRAFKVSGSGIGSVEDLLFVLQYVDAALIGTAFMMAENTEEFVQTVCGGEKMIEDVLRGLDFDKA
YELAKTLPELDEIKIAAVLAALEAKGYGAEVIAGFAKGVAEKSKIEIGKVMDTCGTGGDKTSSINVSTAVAIALSTVHPV
AKHGNRAVSSKSGSADVLEALGVRIEMDEERARKMIAETNFAFLFAPLYHKSFARVAAVRRNLGIRTIFNVTGPLTNPAR
PEVQIVGVASEILLVEVAKAMSLLGRRAVVVYGSGMDEVNPNSSTDIAVVNGGVERLKLEPEDFGIERCRVLPCSSSGES
AERIRAVFSGKGLKEDRRLIAINFATALFALGYEDLKENVEIFEEKVQSGELARKLEEIACKSTSM

Specific function: Bifunctional enzyme that catalyzes the second and fourth steps of tryptophan biosynthetic pathway. The second step is catalyzed by the anthranilate phosphoribosyltransferase, coded by the trpD domain and the fourth step is catalyzed by indole-3- glycerol

COG id: COG0547

COG function: function code E; Anthranilate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the anthranilate phosphoribosyltransferase family

Homologues:

Organism=Escherichia coli, GI1787517, Length=302, Percent_Identity=36.4238410596026, Blast_Score=156, Evalue=4e-39,
Organism=Escherichia coli, GI87081863, Length=246, Percent_Identity=29.6747967479675, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320561, Length=323, Percent_Identity=34.0557275541796, Blast_Score=140, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6322638, Length=233, Percent_Identity=30.9012875536481, Blast_Score=74, Evalue=7e-14,

Paralogues:

None

Copy number: 945 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPCD_ARCFU (O28668)

Other databases:

- EMBL:   AE000782
- PIR:   C69450
- RefSeq:   NP_070433.1
- ProteinModelPortal:   O28668
- SMR:   O28668
- GeneID:   1484830
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF1604
- NMPDR:   fig|224325.1.peg.1594
- TIGR:   AF_1604
- BioCyc:   AFUL224325:AF_1604-MONOMER
- BRENDA:   2.4.2.18
- BRENDA:   4.1.1.48
- HAMAP:   MF_00134_A
- HAMAP:   MF_00211
- InterPro:   IPR013785
- InterPro:   IPR005940
- InterPro:   IPR000312
- InterPro:   IPR017459
- InterPro:   IPR020072
- InterPro:   IPR013798
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- Gene3D:   G3DSA:1.20.970.10
- Gene3D:   G3DSA:3.40.1030.10
- TIGRFAMs:   TIGR01245

Pfam domain/function: PF02885 Glycos_trans_3N; PF00591 Glycos_transf_3; PF00218 IGPS; SSF52418 Glyco_trans_3; SSF51366 RibP_bind_barrel

EC number: =4.1.1.48; =2.4.2.18

Molecular weight: Translated: 59329; Mature: 59329

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMDFGFVDSLKGASKRGKNAVIAEVKVRSPIHGDLLRGRRIEDILRAYEKAGAAAISYIT
CCCCCCHHHHCCCCCCCCCCEEEEEEECCCCCHHHHCCCCHHHHHHHHHHCCHHHHHHHH
AEQFSGNFETLKKIVGLTDLPVLRKDFIRGRKEVERTAEVEAAALLLIARHLKERTAEMV
HHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DFCFEHGIEPLVEVHHAEDLVYAENARAVLINNRDIDRMERDGGSIDVTAKIAEKIRAFK
HHHHHCCCHHHHHHHHHHCEEEECCCCEEEEECCCHHHHHCCCCCEEEHHHHHHHHHHEE
VSGSGIGSVEDLLFVLQYVDAALIGTAFMMAENTEEFVQTVCGGEKMIEDVLRGLDFDKA
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHCCCHHHH
YELAKTLPELDEIKIAAVLAALEAKGYGAEVIAGFAKGVAEKSKIEIGKVMDTCGTGGDK
HHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TSSINVSTAVAIALSTVHPVAKHGNRAVSSKSGSADVLEALGVRIEMDEERARKMIAETN
CCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCEEEECHHHHHHHHHHCC
FAFLFAPLYHKSFARVAAVRRNLGIRTIFNVTGPLTNPARPEVQIVGVASEILLVEVAKA
HHEEEHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEHHHHHHHHHHHHH
MSLLGRRAVVVYGSGMDEVNPNSSTDIAVVNGGVERLKLEPEDFGIERCRVLPCSSSGES
HHHHCCCEEEEEECCCCCCCCCCCCCEEEEECCHHHEEECCHHCCCCCEEEEECCCCCCH
AERIRAVFSGKGLKEDRRLIAINFATALFALGYEDLKENVEIFEEKVQSGELARKLEEIA
HHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHCHHHHHHHHHHHHHHHHCCHHHHHHHHHH
CKSTSM
HHCCCC
>Mature Secondary Structure
MMDFGFVDSLKGASKRGKNAVIAEVKVRSPIHGDLLRGRRIEDILRAYEKAGAAAISYIT
CCCCCCHHHHCCCCCCCCCCEEEEEEECCCCCHHHHCCCCHHHHHHHHHHCCHHHHHHHH
AEQFSGNFETLKKIVGLTDLPVLRKDFIRGRKEVERTAEVEAAALLLIARHLKERTAEMV
HHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DFCFEHGIEPLVEVHHAEDLVYAENARAVLINNRDIDRMERDGGSIDVTAKIAEKIRAFK
HHHHHCCCHHHHHHHHHHCEEEECCCCEEEEECCCHHHHHCCCCCEEEHHHHHHHHHHEE
VSGSGIGSVEDLLFVLQYVDAALIGTAFMMAENTEEFVQTVCGGEKMIEDVLRGLDFDKA
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHCCCHHHH
YELAKTLPELDEIKIAAVLAALEAKGYGAEVIAGFAKGVAEKSKIEIGKVMDTCGTGGDK
HHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TSSINVSTAVAIALSTVHPVAKHGNRAVSSKSGSADVLEALGVRIEMDEERARKMIAETN
CCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCEEEECHHHHHHHHHHCC
FAFLFAPLYHKSFARVAAVRRNLGIRTIFNVTGPLTNPARPEVQIVGVASEILLVEVAKA
HHEEEHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEHHHHHHHHHHHHH
MSLLGRRAVVVYGSGMDEVNPNSSTDIAVVNGGVERLKLEPEDFGIERCRVLPCSSSGES
HHHHCCCEEEEEECCCCCCCCCCCCCEEEEECCHHHEEECCHHCCCCCEEEEECCCCCCH
AERIRAVFSGKGLKEDRRLIAINFATALFALGYEDLKENVEIFEEKVQSGELARKLEEIA
HHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHCHHHHHHHHHHHHHHHHCCHHHHHHHHHH
CKSTSM
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475