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The map label for this gene is mer-2

Identifier: 11498796

GI number: 11498796

Start: 1067239

End: 1068285

Strand: Reverse

Name: mer-2

Synonym: AF1196

Alternate gene names: 11498796

Gene position: 1068285-1067239 (Counterclockwise)

Preceding gene: 11498797

Following gene: 11498795

Centisome position: 49.04

GC content: 51.1

Gene sequence:

>1047_bases
ATGAGCGTCAGATTCGGCATGCTCCTCTCACCTCCGATGCCCGCGCTTGAAAAGGTTATTAAGATTGCCCAGCAGGCTGA
GGACAGCGGGATGTTCTCTTTAACGGTGCCGGACCACACTCTTATGGTTCCCCCAGGCTTTACGCCAAACGCCCTTTCCC
TGCTGTCCGCTTTAGCAGTCAAAACTTCAAAGGCTATGCTGGGAACGGGCGTGACCGATGTTGTTCGCTACCATCCCTCC
GTTCTGGCTCAGATTATGGCCACCATAGACCACCTCTCGTATGGGAGGGCTTTCCTTGGGCTCGGTGCAGGAGAGGCGAT
GAACATAAAGCCCTTCGGCATAGAGTGGAAAAAGCCCTACACGACGCTGAGAGAGGGCATAGAGATCATAAAGAGGCTGT
GGGCAGGAGATAGATTCAGCTACGAGGGAAAAAGGTTCAGGCTGGATGATGCCTTCCTTCAAATAAAGCCGCTACAGAAG
ATTCCCATTTATCTCGGAGCAAATGGAGTCAAAACGAGAGAGCTCGCTGGAGAGATATGCGACGGATGGATGCCCATTGC
AGAAACGCCGAAGACGTACAGGGAGAACCTAAAAGACGTTGAGCGCGGATGCAGTAAGGCTGGAAGAAATCTTGATGAAA
TCGACACGGCGTTGCAGATATACACCGCAGTTGATACATCTTTTGAGAGGGCAATGGAGAGGGCGAGGCAGTTCAGCGGT
GTGATTGTTTCTGCGGCAGAAAAGGCGGAGCAGGCGGGATACAGCCTCGAAATGCCGGAAGGCATATCAAAAAAATTCTA
CTTCGAGCAACTTCTTCTTGATGACAAAATGCTTATGGAGTTCGTAAAGCTTTCATCCCTTGTCACCGAGGAGATGATAA
GGGACTTCTTCATCGTTGGAACTCCTGAGGACTGCGAGGAAAAGATTGAGGAGTTCGTGAAGGCGGGTGTGAAGCACTTC
ATGCTCATAAACGTCGGCCCCGACCCCAAGTACGTCCTGAAGGTGTATGCGGAGAGAATCATTCCGGCATTCTCTGAGAT
AAAGTGA

Upstream 100 bases:

>100_bases
GGCAGGGAATGGCGATGGTTGTTGAGAGAGTCTGATTTTAATTTTTATTTTTTCCAACAGATAGTTAAAGTATAAGTAAT
GAGAAATCTCCCTGTTGGCT

Downstream 100 bases:

>100_bases
TATACTCCTTTGGACGACTGGATTTGAATGCTGTGGGTTGAGAAGTATCGACCGAAAACTCTTGAAGAAGTCGTTGCAGA
CAAGAGCATAATCACCAGGG

Product: N5,N10-methylenetetrahydromethanopterin reductase (mer-2)

Products: NA

Alternate protein names: Coenzyme F420-dependent N(5),N(10)-methylenetetrahydromethanopterin reductase; Methylene-H(4)MPT reductase [H]

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MSVRFGMLLSPPMPALEKVIKIAQQAEDSGMFSLTVPDHTLMVPPGFTPNALSLLSALAVKTSKAMLGTGVTDVVRYHPS
VLAQIMATIDHLSYGRAFLGLGAGEAMNIKPFGIEWKKPYTTLREGIEIIKRLWAGDRFSYEGKRFRLDDAFLQIKPLQK
IPIYLGANGVKTRELAGEICDGWMPIAETPKTYRENLKDVERGCSKAGRNLDEIDTALQIYTAVDTSFERAMERARQFSG
VIVSAAEKAEQAGYSLEMPEGISKKFYFEQLLLDDKMLMEFVKLSSLVTEEMIRDFFIVGTPEDCEEKIEEFVKAGVKHF
MLINVGPDPKYVLKVYAERIIPAFSEIK

Sequences:

>Translated_348_residues
MSVRFGMLLSPPMPALEKVIKIAQQAEDSGMFSLTVPDHTLMVPPGFTPNALSLLSALAVKTSKAMLGTGVTDVVRYHPS
VLAQIMATIDHLSYGRAFLGLGAGEAMNIKPFGIEWKKPYTTLREGIEIIKRLWAGDRFSYEGKRFRLDDAFLQIKPLQK
IPIYLGANGVKTRELAGEICDGWMPIAETPKTYRENLKDVERGCSKAGRNLDEIDTALQIYTAVDTSFERAMERARQFSG
VIVSAAEKAEQAGYSLEMPEGISKKFYFEQLLLDDKMLMEFVKLSSLVTEEMIRDFFIVGTPEDCEEKIEEFVKAGVKHF
MLINVGPDPKYVLKVYAERIIPAFSEIK
>Mature_347_residues
SVRFGMLLSPPMPALEKVIKIAQQAEDSGMFSLTVPDHTLMVPPGFTPNALSLLSALAVKTSKAMLGTGVTDVVRYHPSV
LAQIMATIDHLSYGRAFLGLGAGEAMNIKPFGIEWKKPYTTLREGIEIIKRLWAGDRFSYEGKRFRLDDAFLQIKPLQKI
PIYLGANGVKTRELAGEICDGWMPIAETPKTYRENLKDVERGCSKAGRNLDEIDTALQIYTAVDTSFERAMERARQFSGV
IVSAAEKAEQAGYSLEMPEGISKKFYFEQLLLDDKMLMEFVKLSSLVTEEMIRDFFIVGTPEDCEEKIEEFVKAGVKHFM
LINVGPDPKYVLKVYAERIIPAFSEIK

Specific function: Catalyzes the reversible reduction of methylene-H(4)MPT to methyl-H(4)MPT [H]

COG id: COG2141

COG function: function code C; Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mer family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011251
- InterPro:   IPR016048
- InterPro:   IPR019946 [H]

Pfam domain/function: PF00296 Bac_luciferase [H]

EC number: =1.5.99.11 [H]

Molecular weight: Translated: 38953; Mature: 38822

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVRFGMLLSPPMPALEKVIKIAQQAEDSGMFSLTVPDHTLMVPPGFTPNALSLLSALAV
CCCEECCEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCCHHHHHHHHHHHH
KTSKAMLGTGVTDVVRYHPSVLAQIMATIDHLSYGRAFLGLGAGEAMNIKPFGIEWKKPY
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCCCCCCCCCCHH
TTLREGIEIIKRLWAGDRFSYEGKRFRLDDAFLQIKPLQKIPIYLGANGVKTRELAGEIC
HHHHHHHHHHHHHHCCCCCCCCCCEEEECHHHEEEECCCCCCEEECCCCCCHHHHHHHHH
DGWMPIAETPKTYRENLKDVERGCSKAGRNLDEIDTALQIYTAVDTSFERAMERARQFSG
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VIVSAAEKAEQAGYSLEMPEGISKKFYFEQLLLDDKMLMEFVKLSSLVTEEMIRDFFIVG
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEC
TPEDCEEKIEEFVKAGVKHFMLINVGPDPKYVLKVYAERIIPAFSEIK
CCHHHHHHHHHHHHHCHHEEEEEECCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SVRFGMLLSPPMPALEKVIKIAQQAEDSGMFSLTVPDHTLMVPPGFTPNALSLLSALAV
CCEECCEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCCHHHHHHHHHHHH
KTSKAMLGTGVTDVVRYHPSVLAQIMATIDHLSYGRAFLGLGAGEAMNIKPFGIEWKKPY
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCCCCCCCCCCHH
TTLREGIEIIKRLWAGDRFSYEGKRFRLDDAFLQIKPLQKIPIYLGANGVKTRELAGEIC
HHHHHHHHHHHHHHCCCCCCCCCCEEEECHHHEEEECCCCCCEEECCCCCCHHHHHHHHH
DGWMPIAETPKTYRENLKDVERGCSKAGRNLDEIDTALQIYTAVDTSFERAMERARQFSG
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VIVSAAEKAEQAGYSLEMPEGISKKFYFEQLLLDDKMLMEFVKLSSLVTEEMIRDFFIVG
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEC
TPEDCEEKIEEFVKAGVKHFMLINVGPDPKYVLKVYAERIIPAFSEIK
CCHHHHHHHHHHHHHCHHEEEEEECCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]