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Identifier: 11498795

GI number: 11498795

Start: 1065772

End: 1067211

Strand: Reverse

Name: Not Available

Synonym: AF1195

Alternate gene names: 11498795

Gene position: 1067211-1065772 (Counterclockwise)

Preceding gene: 11498796

Following gene: 11498794

Centisome position: 48.99

GC content: 47.57

Gene sequence:

>1440_bases
ATGCTGTGGGTTGAGAAGTATCGACCGAAAACTCTTGAAGAAGTCGTTGCAGACAAGAGCATAATCACCAGGGTCATTAA
ATGGGCTAAAAGCTGGAAGAGAGGAAGTAAGCCCCTTCTTCTCGCCGGTCCTCCCGGCGTGGGAAAAACGTCCCTCGCTC
TGGCCCTTGCCAACACAATGGGCTGGGAGGCTGTTGAGCTTAACGCCAGTGACCAGAGGAGCTGGAGGGTTATAGAAAGA
ATTGTTGGAGAAGGAGCGTTTAACGAGACAATAAGCGACGAGGGGGAGTTTCTTTCATCAAGAATCGGGAAGCTGAAGCT
AATTATTCTTGACGAGGTTGACAACATTCACAAGAAGGAGGATGTTGGGGGAGAGGCTGCACTAATAAGGCTCATTAAAA
GGAAGCCCGCACAACCCTTAATTCTGATTGCCAACGACCCTTACAAGCTCTCCCCTGAGCTGAGGAATCTTTGCGAGATG
ATTAACTTCAAAAGGCTAACAAAACAGCAGGTTGCCAGAGTTCTTGAGAGAATTGCTTTGAAGGAGGGAATTAAGGTAGA
TAAAAGCGTTCTTCTAAAAATAGCTGAGAACGCCGGAGGAGATTTAAGAGCTGCAATTAACGATTTTCAAGCCCTCGCAG
AGGGGAAAGAGGAGCTGAAACCGGAAGATGTCTTCTTAACGAAGAGAACGCAGGAAAAAGACATTTTCAGAGTAATGCAA
ATGATTTTCAAAACAAAAAATCCGGCTGTTTACAATGAGGCCATGCTTCTTGACGAGTCTCCGGAAGACGTCATCCACTG
GGTTGACGAGAACCTGCCCCTGGAGTACTCTGGCGTTGAGCTGGTCAACGCTTACGAGGCGCTTTCAAGAGCAGATATTT
TTCTCGGTAGAGTTAGGAGAAGGCAGTTCTACAGGCTGTGGAAGTACGCAAGCTATCTCATGACCGTCGGCGTTCAGCAA
ATGAAGGAGGAACCCAAGAAAGGCTTCACTAGGTACAGAAGGCCGGCCGTCTGGCAGATGCTCTTCCAGCTCAGGCAGAA
AAGGGAAATGACGAGAAAAATACTCGAAAAGATTGGGAAGTACAGCCACCTCTCTATGAGAAAAGCCCGCACCGAAATGT
TCCCCGTGATAAAGCTGCTCTTAAAGGAGCTTGATGTGGACAAAGCTGCAACCATCGCGGCCTTCTACGAGTTCACAAAG
GAGGAACTGGAATTCCTTGTTGGGGAGAAGGGGGACGAAATCTGGAAATACGTTGAGAAGCACGGAATGCACAGAATTGA
GGACGAGACCTTCCTTGAAAGCTTTGTGAAAGCTGAAAAGGAAGAGAAGGAGGAGAGTGTTGAGGAAGTTGCTGAGGAGA
AACCTGAAGAAGAGAGAGAGGAGCCAAGAGCGAGAAAGAAAGCCGGAAAAAATTTAACTCTCGATTCCTTCTTTTCCTGA

Upstream 100 bases:

>100_bases
CGGCCCCGACCCCAAGTACGTCCTGAAGGTGTATGCGGAGAGAATCATTCCGGCATTCTCTGAGATAAAGTGATATACTC
CTTTGGACGACTGGATTTGA

Downstream 100 bases:

>100_bases
ATGATGCGCAAGGTGATCGCTGCAGCCTTCAAGAGCAAGGGAAAAAAGAAAATGAAAAGAAGCGAGTTGATTTATACCAT
GAGCTTCGATTTGAACTGGT

Product: replication factor C large subunit

Products: N Pyrophosphate; DNA(N). [C]

Alternate protein names: RFC large subunit; Clamp loader large subunit; afRFC large subunit; afRFCla

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MLWVEKYRPKTLEEVVADKSIITRVIKWAKSWKRGSKPLLLAGPPGVGKTSLALALANTMGWEAVELNASDQRSWRVIER
IVGEGAFNETISDEGEFLSSRIGKLKLIILDEVDNIHKKEDVGGEAALIRLIKRKPAQPLILIANDPYKLSPELRNLCEM
INFKRLTKQQVARVLERIALKEGIKVDKSVLLKIAENAGGDLRAAINDFQALAEGKEELKPEDVFLTKRTQEKDIFRVMQ
MIFKTKNPAVYNEAMLLDESPEDVIHWVDENLPLEYSGVELVNAYEALSRADIFLGRVRRRQFYRLWKYASYLMTVGVQQ
MKEEPKKGFTRYRRPAVWQMLFQLRQKREMTRKILEKIGKYSHLSMRKARTEMFPVIKLLLKELDVDKAATIAAFYEFTK
EELEFLVGEKGDEIWKYVEKHGMHRIEDETFLESFVKAEKEEKEESVEEVAEEKPEEEREEPRARKKAGKNLTLDSFFS

Sequences:

>Translated_479_residues
MLWVEKYRPKTLEEVVADKSIITRVIKWAKSWKRGSKPLLLAGPPGVGKTSLALALANTMGWEAVELNASDQRSWRVIER
IVGEGAFNETISDEGEFLSSRIGKLKLIILDEVDNIHKKEDVGGEAALIRLIKRKPAQPLILIANDPYKLSPELRNLCEM
INFKRLTKQQVARVLERIALKEGIKVDKSVLLKIAENAGGDLRAAINDFQALAEGKEELKPEDVFLTKRTQEKDIFRVMQ
MIFKTKNPAVYNEAMLLDESPEDVIHWVDENLPLEYSGVELVNAYEALSRADIFLGRVRRRQFYRLWKYASYLMTVGVQQ
MKEEPKKGFTRYRRPAVWQMLFQLRQKREMTRKILEKIGKYSHLSMRKARTEMFPVIKLLLKELDVDKAATIAAFYEFTK
EELEFLVGEKGDEIWKYVEKHGMHRIEDETFLESFVKAEKEEKEESVEEVAEEKPEEEREEPRARKKAGKNLTLDSFFS
>Mature_479_residues
MLWVEKYRPKTLEEVVADKSIITRVIKWAKSWKRGSKPLLLAGPPGVGKTSLALALANTMGWEAVELNASDQRSWRVIER
IVGEGAFNETISDEGEFLSSRIGKLKLIILDEVDNIHKKEDVGGEAALIRLIKRKPAQPLILIANDPYKLSPELRNLCEM
INFKRLTKQQVARVLERIALKEGIKVDKSVLLKIAENAGGDLRAAINDFQALAEGKEELKPEDVFLTKRTQEKDIFRVMQ
MIFKTKNPAVYNEAMLLDESPEDVIHWVDENLPLEYSGVELVNAYEALSRADIFLGRVRRRQFYRLWKYASYLMTVGVQQ
MKEEPKKGFTRYRRPAVWQMLFQLRQKREMTRKILEKIGKYSHLSMRKARTEMFPVIKLLLKELDVDKAATIAAFYEFTK
EELEFLVGEKGDEIWKYVEKHGMHRIEDETFLESFVKAEKEEKEESVEEVAEEKPEEEREEPRARKKAGKNLTLDSFFS

Specific function: Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA. The complex possesses DNA-dependent ATPase activity which is further stimulated by PCNA

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the activator 1 small subunits family. RfcL subfamily

Homologues:

Organism=Homo sapiens, GI32528306, Length=366, Percent_Identity=27.3224043715847, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI27501458, Length=262, Percent_Identity=27.8625954198473, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI6677723, Length=245, Percent_Identity=29.7959183673469, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI31563534, Length=276, Percent_Identity=29.3478260869565, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI194306571, Length=223, Percent_Identity=31.390134529148, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI194306567, Length=223, Percent_Identity=31.390134529148, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI4506491, Length=234, Percent_Identity=37.1794871794872, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI31881687, Length=234, Percent_Identity=37.1794871794872, Blast_Score=82, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17563226, Length=252, Percent_Identity=30.5555555555556, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17554730, Length=220, Percent_Identity=34.0909090909091, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17541988, Length=230, Percent_Identity=30.4347826086957, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71986063, Length=235, Percent_Identity=31.4893617021277, Blast_Score=92, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17541368, Length=251, Percent_Identity=28.2868525896414, Blast_Score=88, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6324791, Length=230, Percent_Identity=31.7391304347826, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324039, Length=217, Percent_Identity=33.1797235023041, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6322528, Length=226, Percent_Identity=32.3008849557522, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324478, Length=290, Percent_Identity=27.9310344827586, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6323724, Length=259, Percent_Identity=29.3436293436293, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17737765, Length=245, Percent_Identity=30.6122448979592, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI62472391, Length=267, Percent_Identity=27.7153558052434, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI28574716, Length=243, Percent_Identity=29.6296296296296, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17647857, Length=296, Percent_Identity=27.3648648648649, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19921076, Length=286, Percent_Identity=29.020979020979, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI18859927, Length=217, Percent_Identity=29.9539170506912, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RFCL_ARCFU (O29072)

Other databases:

- EMBL:   AE000782
- PIR:   B69399
- RefSeq:   NP_070024.1
- ProteinModelPortal:   O29072
- SMR:   O29072
- GeneID:   1484419
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF1195
- NMPDR:   fig|224325.1.peg.1184
- TIGR:   AF_1195
- HOGENOM:   HBG541322
- OMA:   ELNASDK
- ProtClustDB:   PRK04195
- BioCyc:   AFUL224325:AF_1195-MONOMER
- HAMAP:   MF_01508
- InterPro:   IPR003593
- InterPro:   IPR003959
- SMART:   SM00382

Pfam domain/function: PF00004 AAA

EC number: 2.7.7.7 [C]

Molecular weight: Translated: 55512; Mature: 55512

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLWVEKYRPKTLEEVVADKSIITRVIKWAKSWKRGSKPLLLAGPPGVGKTSLALALANTM
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHC
GWEAVELNASDQRSWRVIERIVGEGAFNETISDEGEFLSSRIGKLKLIILDEVDNIHKKE
CCCEEECCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCEEEEEECCHHHHHHHH
DVGGEAALIRLIKRKPAQPLILIANDPYKLSPELRNLCEMINFKRLTKQQVARVLERIAL
CCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KEGIKVDKSVLLKIAENAGGDLRAAINDFQALAEGKEELKPEDVFLTKRTQEKDIFRVMQ
HHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHCCCCCEEEECCCCHHHHHHHHH
MIFKTKNPAVYNEAMLLDESPEDVIHWVDENLPLEYSGVELVNAYEALSRADIFLGRVRR
HHHHCCCCCHHHHHHCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RQFYRLWKYASYLMTVGVQQMKEEPKKGFTRYRRPAVWQMLFQLRQKREMTRKILEKIGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCC
YSHLSMRKARTEMFPVIKLLLKELDVDKAATIAAFYEFTKEELEFLVGEKGDEIWKYVEK
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
HGMHRIEDETFLESFVKAEKEEKEESVEEVAEEKPEEEREEPRARKKAGKNLTLDSFFS
CCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHCCCC
>Mature Secondary Structure
MLWVEKYRPKTLEEVVADKSIITRVIKWAKSWKRGSKPLLLAGPPGVGKTSLALALANTM
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHC
GWEAVELNASDQRSWRVIERIVGEGAFNETISDEGEFLSSRIGKLKLIILDEVDNIHKKE
CCCEEECCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCEEEEEECCHHHHHHHH
DVGGEAALIRLIKRKPAQPLILIANDPYKLSPELRNLCEMINFKRLTKQQVARVLERIAL
CCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KEGIKVDKSVLLKIAENAGGDLRAAINDFQALAEGKEELKPEDVFLTKRTQEKDIFRVMQ
HHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHCCCCCEEEECCCCHHHHHHHHH
MIFKTKNPAVYNEAMLLDESPEDVIHWVDENLPLEYSGVELVNAYEALSRADIFLGRVRR
HHHHCCCCCHHHHHHCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RQFYRLWKYASYLMTVGVQQMKEEPKKGFTRYRRPAVWQMLFQLRQKREMTRKILEKIGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCC
YSHLSMRKARTEMFPVIKLLLKELDVDKAATIAAFYEFTKEELEFLVGEKGDEIWKYVEK
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
HGMHRIEDETFLESFVKAEKEEKEESVEEVAEEKPEEEREEPRARKKAGKNLTLDSFFS
CCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: N Deoxynucleoside Triphosphate; DNA [C]

Specific reaction: N Deoxynucleoside Triphosphate = N Pyrophosphate + DNA(N). Protein + DNA = Protein-DNA [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; 2', 3'-Dideoxythymidine5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate;

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475; 12384579