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The map label for this gene is fadA-2

Identifier: 11498797

GI number: 11498797

Start: 1068351

End: 1069625

Strand: Reverse

Name: fadA-2

Synonym: AF1197

Alternate gene names: 11498797

Gene position: 1069625-1068351 (Counterclockwise)

Preceding gene: 11498798

Following gene: 11498796

Centisome position: 49.1

GC content: 55.22

Gene sequence:

>1275_bases
ATGGCAGTTATTGTAGGAGCGGCAAGAACCCCCGTGGGTAGATTTGGAGGCAGCTTGAAGGATGTAGCTGCATACGAGCT
TGGGGCTGTTGCGATTAAGGGAATGCTGAAAAAGCTGGGCGTTGCACCAAAGCCCACTCCCGAATCCATTGAGTTCTACC
CGGAGAAGCTGGAGAAGGGCAGAATTGATCTGGAGTCGGCCTACGACTACGATGGGGTTGAGGTGGAGATTGACGAAGTC
ATTATGGGAAACGTTTTGCAGGCAGCTCAGGGACAGAATCCAGCGAGGCAGGCGTCAATTCTTGCGGGAATACCAAAGGA
GATTCCCGCTTACACTGTCAACAAGGTTTGCGGGAGCGGCTTGAAGGCCATCGCCCTCGCCTACCACGCCGTAAAGGCTG
GAGACGCTAAGGCAATCATCGCAGGAGGGATGGAGAGCATGAGCAACGCCCCCTACGCCCTGCCAAAGGCGAGGTGGGGA
TACAGGATGAGCGTCACAGCCAAGGACGAGATTCTGGACCTCATGGTTTACGACGGCCTCTGGGAGAAGTTCTACGGCTA
CCACATGGGGATGACAGCCGAAAACATAGTGGAGAGGTACGGCATAAGCAGAGAGGAGCAGGACCAGCTTGCATACGAGA
GCCACATGAGGGCTGTAAGGGCCATTGACGAGGGCAAGTTCGCTCAGGAGATAGTCCCGGTGGAGGTTAAGCAGAAGAAG
GGAGCAGTTGTGGTGGACACCGACGAGCATCCAAGAAGGGACACGAGCCTTGAGAAGCTCGCGAAGCTACCCCCTGTCTT
CAAGAAGGATGGAACTGTCACTGCAGGAAATGCGAGTGGGGTTAATGATGGAGCCGCTGCCGTTCTGGTTATGGACGAGA
AGGCAGCGAAGGACTACGGGCTGGAGCCGAAAGTCAGAATACTTGCTGTTGCTTCAGCGGGCATCGACCCTGCATACATG
GGCCTTGGGCCTATACCAGCCATAAGAAAGGTTCTGAAGAAAGCTGGGCCTGAAATCAAGGACATAGGGCTGATTGAGCT
CAATGAAGCCTTTGCAGCTCAGGCGTTAGCCTGCATAAAGGAGCTTGGACTGGACATGGAAAAGGTCAACGTCAACGGCA
GCGGTATATCCCTCGGCCACCCAATTGGCTGCACAGGGGCGAGGATAACAACAACGCTCATCTACGAGATGGAGAGGAGA
GGGGTGGACTACGGTCTGGCCTCTCTCTGCATCGGCGGTGGGCAGGGAATGGCGATGGTTGTTGAGAGAGTCTGA

Upstream 100 bases:

>100_bases
AGTCGAGTACACCGAACCACCAACAAGAGAGGAAATCGACACACTTAAAGAATTGGACCCGGATGGTATTTTAATAAGAA
AAAAGTGAAGGAGGTGTATT

Downstream 100 bases:

>100_bases
TTTTAATTTTTATTTTTTCCAACAGATAGTTAAAGTATAAGTAATGAGAAATCTCCCTGTTGGCTATGAGCGTCAGATTC
GGCATGCTCCTCTCACCTCC

Product: 3-ketoacyl-CoA thiolase (fadA-2)

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MAVIVGAARTPVGRFGGSLKDVAAYELGAVAIKGMLKKLGVAPKPTPESIEFYPEKLEKGRIDLESAYDYDGVEVEIDEV
IMGNVLQAAQGQNPARQASILAGIPKEIPAYTVNKVCGSGLKAIALAYHAVKAGDAKAIIAGGMESMSNAPYALPKARWG
YRMSVTAKDEILDLMVYDGLWEKFYGYHMGMTAENIVERYGISREEQDQLAYESHMRAVRAIDEGKFAQEIVPVEVKQKK
GAVVVDTDEHPRRDTSLEKLAKLPPVFKKDGTVTAGNASGVNDGAAAVLVMDEKAAKDYGLEPKVRILAVASAGIDPAYM
GLGPIPAIRKVLKKAGPEIKDIGLIELNEAFAAQALACIKELGLDMEKVNVNGSGISLGHPIGCTGARITTTLIYEMERR
GVDYGLASLCIGGGQGMAMVVERV

Sequences:

>Translated_424_residues
MAVIVGAARTPVGRFGGSLKDVAAYELGAVAIKGMLKKLGVAPKPTPESIEFYPEKLEKGRIDLESAYDYDGVEVEIDEV
IMGNVLQAAQGQNPARQASILAGIPKEIPAYTVNKVCGSGLKAIALAYHAVKAGDAKAIIAGGMESMSNAPYALPKARWG
YRMSVTAKDEILDLMVYDGLWEKFYGYHMGMTAENIVERYGISREEQDQLAYESHMRAVRAIDEGKFAQEIVPVEVKQKK
GAVVVDTDEHPRRDTSLEKLAKLPPVFKKDGTVTAGNASGVNDGAAAVLVMDEKAAKDYGLEPKVRILAVASAGIDPAYM
GLGPIPAIRKVLKKAGPEIKDIGLIELNEAFAAQALACIKELGLDMEKVNVNGSGISLGHPIGCTGARITTTLIYEMERR
GVDYGLASLCIGGGQGMAMVVERV
>Mature_423_residues
AVIVGAARTPVGRFGGSLKDVAAYELGAVAIKGMLKKLGVAPKPTPESIEFYPEKLEKGRIDLESAYDYDGVEVEIDEVI
MGNVLQAAQGQNPARQASILAGIPKEIPAYTVNKVCGSGLKAIALAYHAVKAGDAKAIIAGGMESMSNAPYALPKARWGY
RMSVTAKDEILDLMVYDGLWEKFYGYHMGMTAENIVERYGISREEQDQLAYESHMRAVRAIDEGKFAQEIVPVEVKQKKG
AVVVDTDEHPRRDTSLEKLAKLPPVFKKDGTVTAGNASGVNDGAAAVLVMDEKAAKDYGLEPKVRILAVASAGIDPAYMG
LGPIPAIRKVLKKAGPEIKDIGLIELNEAFAAQALACIKELGLDMEKVNVNGSGISLGHPIGCTGARITTTLIYEMERRG
VDYGLASLCIGGGQGMAMVVERV

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=423, Percent_Identity=44.6808510638298, Blast_Score=360, Evalue=2e-99,
Organism=Homo sapiens, GI167614485, Length=424, Percent_Identity=44.3396226415094, Blast_Score=346, Evalue=2e-95,
Organism=Homo sapiens, GI4557237, Length=424, Percent_Identity=41.7452830188679, Blast_Score=298, Evalue=8e-81,
Organism=Homo sapiens, GI4501853, Length=427, Percent_Identity=38.4074941451991, Blast_Score=255, Evalue=7e-68,
Organism=Homo sapiens, GI4504327, Length=452, Percent_Identity=32.9646017699115, Blast_Score=199, Evalue=6e-51,
Organism=Homo sapiens, GI194353979, Length=422, Percent_Identity=30.5687203791469, Blast_Score=156, Evalue=4e-38,
Organism=Escherichia coli, GI1788554, Length=423, Percent_Identity=51.3002364066194, Blast_Score=394, Evalue=1e-111,
Organism=Escherichia coli, GI87082165, Length=421, Percent_Identity=47.5059382422803, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI1787663, Length=428, Percent_Identity=43.6915887850467, Blast_Score=338, Evalue=4e-94,
Organism=Escherichia coli, GI48994986, Length=432, Percent_Identity=41.6666666666667, Blast_Score=286, Evalue=1e-78,
Organism=Escherichia coli, GI1788683, Length=378, Percent_Identity=36.7724867724868, Blast_Score=197, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI133906874, Length=422, Percent_Identity=42.654028436019, Blast_Score=329, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI25147385, Length=423, Percent_Identity=39.2434988179669, Blast_Score=287, Evalue=9e-78,
Organism=Caenorhabditis elegans, GI17535921, Length=423, Percent_Identity=38.0614657210402, Blast_Score=260, Evalue=1e-69,
Organism=Caenorhabditis elegans, GI17551802, Length=452, Percent_Identity=32.5221238938053, Blast_Score=197, Evalue=9e-51,
Organism=Caenorhabditis elegans, GI17535917, Length=431, Percent_Identity=31.554524361949, Blast_Score=184, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6325229, Length=425, Percent_Identity=42.3529411764706, Blast_Score=298, Evalue=1e-81,
Organism=Saccharomyces cerevisiae, GI6322031, Length=420, Percent_Identity=36.6666666666667, Blast_Score=229, Evalue=5e-61,
Organism=Drosophila melanogaster, GI24655093, Length=421, Percent_Identity=45.8432304038005, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI17648125, Length=423, Percent_Identity=40.4255319148936, Blast_Score=307, Evalue=1e-83,
Organism=Drosophila melanogaster, GI24640423, Length=423, Percent_Identity=39.2434988179669, Blast_Score=300, Evalue=9e-82,
Organism=Drosophila melanogaster, GI17137578, Length=400, Percent_Identity=35, Blast_Score=181, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 45241; Mature: 45110

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS00098 THIOLASE_1 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVIVGAARTPVGRFGGSLKDVAAYELGAVAIKGMLKKLGVAPKPTPESIEFYPEKLEKG
CEEEEECCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCHHHHHCC
RIDLESAYDYDGVEVEIDEVIMGNVLQAAQGQNPARQASILAGIPKEIPAYTVNKVCGSG
CCEECCCCCCCCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCC
LKAIALAYHAVKAGDAKAIIAGGMESMSNAPYALPKARWGYRMSVTAKDEILDLMVYDGL
HHHHHHHHHHHHCCCCCEEEECCHHHHCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHH
WEKFYGYHMGMTAENIVERYGISREEQDQLAYESHMRAVRAIDEGKFAQEIVPVEVKQKK
HHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCEEEECCC
GAVVVDTDEHPRRDTSLEKLAKLPPVFKKDGTVTAGNASGVNDGAAAVLVMDEKAAKDYG
CCEEEECCCCCCCCCHHHHHHHCCCCCCCCCCEEECCCCCCCCCCEEEEEEECHHHHCCC
LEPKVRILAVASAGIDPAYMGLGPIPAIRKVLKKAGPEIKDIGLIELNEAFAAQALACIK
CCCCEEEEEEECCCCCHHHCCCCCCHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHHH
ELGLDMEKVNVNGSGISLGHPIGCTGARITTTLIYEMERRGVDYGLASLCIGGGQGMAMV
HHCCCEEEEECCCCCEECCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCEEH
VERV
EECC
>Mature Secondary Structure 
AVIVGAARTPVGRFGGSLKDVAAYELGAVAIKGMLKKLGVAPKPTPESIEFYPEKLEKG
EEEEECCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCHHHHHCC
RIDLESAYDYDGVEVEIDEVIMGNVLQAAQGQNPARQASILAGIPKEIPAYTVNKVCGSG
CCEECCCCCCCCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCC
LKAIALAYHAVKAGDAKAIIAGGMESMSNAPYALPKARWGYRMSVTAKDEILDLMVYDGL
HHHHHHHHHHHHCCCCCEEEECCHHHHCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHH
WEKFYGYHMGMTAENIVERYGISREEQDQLAYESHMRAVRAIDEGKFAQEIVPVEVKQKK
HHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCEEEECCC
GAVVVDTDEHPRRDTSLEKLAKLPPVFKKDGTVTAGNASGVNDGAAAVLVMDEKAAKDYG
CCEEEECCCCCCCCCHHHHHHHCCCCCCCCCCEEECCCCCCCCCCEEEEEEECHHHHCCC
LEPKVRILAVASAGIDPAYMGLGPIPAIRKVLKKAGPEIKDIGLIELNEAFAAQALACIK
CCCCEEEEEEECCCCCHHHCCCCCCHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHHH
ELGLDMEKVNVNGSGISLGHPIGCTGARITTTLIYEMERRGVDYGLASLCIGGGQGMAMV
HHCCCEEEEECCCCCEECCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCEEH
VERV
EECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]