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The map label for this gene is eif2A

Identifier: 11498138

GI number: 11498138

Start: 479725

End: 480528

Strand: Reverse

Name: eif2A

Synonym: AF0527

Alternate gene names: 11498138

Gene position: 480528-479725 (Counterclockwise)

Preceding gene: 11498139

Following gene: 11498137

Centisome position: 22.06

GC content: 47.51

Gene sequence:

>804_bases
ATGAAAGAAAAGAGGCTAATCATAAAGAGAAGTGGTTATCCTTCTAAGGGCGAGATTGTCATAGGAACGGTTAAGAGGGT
GCTCGATTTTGGAGCCTTTGTTTCCCTTGATGAGTACGAGGGCAGAGAAGGGATGGTTCACATTAGCGAGGTCGCGTCAG
GTTGGATTAAGGACATCAGGGAGCACGTCAAAAAAGGACAGAAGGTAATATGCAAGGTACTCGATGTAAATCCGAAAAGA
GGGCACATTGACCTCTCGATAAAGGACGTCAACGAGAGGCAGAGAAGGGAGAAGCTTCAGCAGTGGAAGAACGAGATGAA
GGCATTCAAGTGGCTCGAAATAATCGGCGAAAAGCTGAATATCGATTTCAAGGAGCTTGAGAAAATCGGGAAGAAACTCA
TGAAGGAGTACGATTCCGTTTACTCCGCCTTCGAGGAGGCTGCTTTTGAGGGATATGAGGTTCTCGCTCCCATTGTAGGG
GAAGAGTTTGCCAAGGAGATGGCGGAGATAGCCAGGGAGAACATAAAGCCCAAAAGGGTTAAGGTTAGAGGGTACTTCGA
GCTTAAGAGCAGCGCTTCTGACGGAATCGAGAGGATAAAAAAGGCCCTTCTTGAGGCCAAAAAGGCTCTGGTAAATGGTG
TGGAGATGAAGCTTGAGTACGTCGGCGCACCCAAGTACAGAATCGTCGTAGAGGCGGATGATTACAAAACCGCCGAAAAC
GTTTTGAAGAAGGCCACAGAAAATGTACTGAAAGCGATCAAAAAGCTTGGTGGAGAGGGCAACTTCATCAGGGAAGCGGC
ATGA

Upstream 100 bases:

>100_bases
AGAGAATGGTCTGAGAGTTTGCGCTGATGGGCCGGTTTTTAATGCCTCCGAACTGCCGTGGTAACAATATTTAAATTCAC
TGAAATAGGAAGGGAGAAGG

Downstream 100 bases:

>100_bases
AGGTTTTGATGCGAAAGTGCGGAAAATGCGGAAGGTACACGCTGAAGGAGAGATGTCCTGTGTGCGGTGAGAGGACTCAC
ATGCCAATCCCACCGAGATT

Product: translation initiation factor IF-2 subunit alpha

Products: RNAn; a nucleoside diphosphate [C]

Alternate protein names: aIF2-alpha; eIF-2-alpha

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MKEKRLIIKRSGYPSKGEIVIGTVKRVLDFGAFVSLDEYEGREGMVHISEVASGWIKDIREHVKKGQKVICKVLDVNPKR
GHIDLSIKDVNERQRREKLQQWKNEMKAFKWLEIIGEKLNIDFKELEKIGKKLMKEYDSVYSAFEEAAFEGYEVLAPIVG
EEFAKEMAEIARENIKPKRVKVRGYFELKSSASDGIERIKKALLEAKKALVNGVEMKLEYVGAPKYRIVVEADDYKTAEN
VLKKATENVLKAIKKLGGEGNFIREAA

Sequences:

>Translated_267_residues
MKEKRLIIKRSGYPSKGEIVIGTVKRVLDFGAFVSLDEYEGREGMVHISEVASGWIKDIREHVKKGQKVICKVLDVNPKR
GHIDLSIKDVNERQRREKLQQWKNEMKAFKWLEIIGEKLNIDFKELEKIGKKLMKEYDSVYSAFEEAAFEGYEVLAPIVG
EEFAKEMAEIARENIKPKRVKVRGYFELKSSASDGIERIKKALLEAKKALVNGVEMKLEYVGAPKYRIVVEADDYKTAEN
VLKKATENVLKAIKKLGGEGNFIREAA
>Mature_267_residues
MKEKRLIIKRSGYPSKGEIVIGTVKRVLDFGAFVSLDEYEGREGMVHISEVASGWIKDIREHVKKGQKVICKVLDVNPKR
GHIDLSIKDVNERQRREKLQQWKNEMKAFKWLEIIGEKLNIDFKELEKIGKKLMKEYDSVYSAFEEAAFEGYEVLAPIVG
EEFAKEMAEIARENIKPKRVKVRGYFELKSSASDGIERIKKALLEAKKALVNGVEMKLEYVGAPKYRIVVEADDYKTAEN
VLKKATENVLKAIKKLGGEGNFIREAA

Specific function: eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA

COG id: COG1093

COG function: function code J; Translation initiation factor 2, alpha subunit (eIF-2alpha)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI4758256, Length=242, Percent_Identity=22.3140495867769, Blast_Score=68, Evalue=7e-12,
Organism=Escherichia coli, GI145693187, Length=79, Percent_Identity=40.5063291139241, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17509871, Length=262, Percent_Identity=24.0458015267176, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6322466, Length=256, Percent_Identity=28.515625, Blast_Score=101, Evalue=1e-22,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): IF2A_ARCFU (O29723)

Other databases:

- EMBL:   AE000782
- PIR:   G69315
- RefSeq:   NP_069363.1
- ProteinModelPortal:   O29723
- SMR:   O29723
- GeneID:   1483744
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF0527
- NMPDR:   fig|224325.1.peg.522
- TIGR:   AF_0527
- HOGENOM:   HBG496806
- OMA:   EYPGKEG
- ProtClustDB:   PRK03987
- BioCyc:   AFUL224325:AF_0527-MONOMER
- HAMAP:   MF_00231
- InterPro:   IPR011488
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR022964
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00316

Pfam domain/function: PF07541 EIF_2_alpha; PF00575 S1; SSF50249 Nucleic_acid_OB

EC number: 2.7.7.8 [C]

Molecular weight: Translated: 30509; Mature: 30509

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEKRLIIKRSGYPSKGEIVIGTVKRVLDFGAFVSLDEYEGREGMVHISEVASGWIKDIR
CCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHHEEEHHCCCCCCCEEHHHHHHHHHHHHH
EHVKKGQKVICKVLDVNPKRGHIDLSIKDVNERQRREKLQQWKNEMKAFKWLEIIGEKLN
HHHHHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
IDFKELEKIGKKLMKEYDSVYSAFEEAAFEGYEVLAPIVGEEFAKEMAEIARENIKPKRV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
KVRGYFELKSSASDGIERIKKALLEAKKALVNGVEMKLEYVGAPKYRIVVEADDYKTAEN
EEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEEECCCCHHHHH
VLKKATENVLKAIKKLGGEGNFIREAA
HHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKEKRLIIKRSGYPSKGEIVIGTVKRVLDFGAFVSLDEYEGREGMVHISEVASGWIKDIR
CCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHHEEEHHCCCCCCCEEHHHHHHHHHHHHH
EHVKKGQKVICKVLDVNPKRGHIDLSIKDVNERQRREKLQQWKNEMKAFKWLEIIGEKLN
HHHHHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
IDFKELEKIGKKLMKEYDSVYSAFEEAAFEGYEVLAPIVGEEFAKEMAEIARENIKPKRV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
KVRGYFELKSSASDGIERIKKALLEAKKALVNGVEMKLEYVGAPKYRIVVEADDYKTAEN
EEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEEECCCCHHHHH
VLKKATENVLKAIKKLGGEGNFIREAA
HHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 4500 [C]

Specific activity: NA

Km value (mM): NA

Substrates: RNAn+1; phosphate [C]

Specific reaction: RNAn+1 + phosphate = RNAn + a nucleoside diphosphate [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475