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Identifier: 15679561

GI number: 15679561

Start: 1431195

End: 1432631

Strand: Direct

Name: Not Available

Synonym: MTH1565

Alternate gene names: 15679561

Gene position: 1431195-1432631 (Clockwise)

Preceding gene: 15679560

Following gene: 15679562

Centisome position: 81.72

GC content: 44.95

Gene sequence:

>1437_bases
ATGAACCACAGCACTGGAAACTCCAGCAATAATAAAGAATTTCCTGGAAGTAACCTTGAATTCCAGGTGGGGCATGTTGA
TAGGGTAATCGACACCATCCCCTCAAGGAGAGGACACTTCAAAAGGAGTGGAGGGGGAGGTCCATGCTGCAATAAGGCAT
CAGTACCGGGTATGGTTACTCAGAGATCATGTGTATACGGAGGCGCAAGGGTTGTTCTTATGCCGATAACAGATGCAGCT
CACATAGTCCATGGACCTGTTGGATGCGCGGCATGTACATGGGATATAAGGGGCAGCAGAAGTTCAAAGTCAGATCTGTA
CAAGACGGGATTCTCCACGAACCTTGAGGAGAAGGATGTGGTCTTTGGCGGTGAGGACAGACTTCTCAAATGCATACTTG
AAGTGGACGAAATTTACTCTCCATCAGCAATATTCGTGTACTCCACATGTGTTTCAGGTGTCATAGGCGATGATATCACC
GCCGTATGCAGAAATGCAGAATATAAAACGGGAAAATGGGTAATACCCGTACAGTCTGAGGGTTTTAGGAGTTTCAATAA
ATCCTTAGGCCATCAGCTTGCCTGTGATGTCCTTCTTGATCACGTTATAGGTCGTGGAGATGCCGAAGTAGGGGAAAGGT
CAGTTAATCTTGTGGGCGAATTCAATGTGGCGGGGGATTTATGGGAGATAAAGAGGCTCCTTGAGAAAATGGGCGTTAAG
GTTGTTGCATCCATAACCGGTGATTCAACCGTGGCTGAGATTTCAGCCGCCCACAAAGCCCAGCTTAACCTTGTACAGTG
CAGCAAATCATCTGGATACATCGCAGAGGAGATGGAAAAAAGGCATGGAACACCACACATCAACGTCAATTTTTTTGGTA
TGAGGGAAACCATGGATTCCCTCATTTCAATTTCAGAGTTCTTTGGACTGGAATTAGCAGAGAAATGGGTACTTGGAATG
GTTGAGGAAACAAAGAGGAAGGTTGCCAGATACAGGAATACCCTTGAGGGAAAGAAGGTGGCCATATACGTAGGAGGTAA
CAAGGCGTGGTCACTCATAAGGGCATTTGAAGATCTGGGAATGGAGGTTATCATGAGCGGAACCCAGAATGGTCTGCCCA
ACGATTACCTTCGAATAATGGAGAAATCTGGCAGAGAGCTCATACTATTCGATGACGCCAATCCAGTTGAACTTTCAAGG
TTGTTGAATAAATATCGACCAGATCTTTTTGTCTCAGGAGCTAAAGAGAAATACCTCTCATACAAACTTGGCATACCTTT
CTGTGAATTTAATCATGACAGGATAAAGCCCTTTTCATGTTTCAGGGGATTTTTGAATTTTGCAGAGGAAGTTGATAAGG
CTGTAAACAGCAGAGTATGGAAACTGGCAGCACCAAGAACCTTTCTAAATTGCGGAGTTGAGTACGGTTATGAATAG

Upstream 100 bases:

>100_bases
GTATATGATAGAGTTGGGTATCACAGGATTCCTGTTGTGGGTTACCGTGGATCTGTCAATCTTCTAAACAGAATAACAAA
CACAGTACTGAGAGAATACT

Downstream 100 bases:

>100_bases
AAACATAACAATAAACCCCGGAAGGATCTGCCAGCCACTTGGCGCCGTCATGGCTTTCCTTGGAGTCAGGGGAGCGATGC
CACTAATACATGGTTCGCAG

Product: nitrogenase iron-molybdenum cofactor biosynthesis protein NifE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MNHSTGNSSNNKEFPGSNLEFQVGHVDRVIDTIPSRRGHFKRSGGGGPCCNKASVPGMVTQRSCVYGGARVVLMPITDAA
HIVHGPVGCAACTWDIRGSRSSKSDLYKTGFSTNLEEKDVVFGGEDRLLKCILEVDEIYSPSAIFVYSTCVSGVIGDDIT
AVCRNAEYKTGKWVIPVQSEGFRSFNKSLGHQLACDVLLDHVIGRGDAEVGERSVNLVGEFNVAGDLWEIKRLLEKMGVK
VVASITGDSTVAEISAAHKAQLNLVQCSKSSGYIAEEMEKRHGTPHINVNFFGMRETMDSLISISEFFGLELAEKWVLGM
VEETKRKVARYRNTLEGKKVAIYVGGNKAWSLIRAFEDLGMEVIMSGTQNGLPNDYLRIMEKSGRELILFDDANPVELSR
LLNKYRPDLFVSGAKEKYLSYKLGIPFCEFNHDRIKPFSCFRGFLNFAEEVDKAVNSRVWKLAAPRTFLNCGVEYGYE

Sequences:

>Translated_478_residues
MNHSTGNSSNNKEFPGSNLEFQVGHVDRVIDTIPSRRGHFKRSGGGGPCCNKASVPGMVTQRSCVYGGARVVLMPITDAA
HIVHGPVGCAACTWDIRGSRSSKSDLYKTGFSTNLEEKDVVFGGEDRLLKCILEVDEIYSPSAIFVYSTCVSGVIGDDIT
AVCRNAEYKTGKWVIPVQSEGFRSFNKSLGHQLACDVLLDHVIGRGDAEVGERSVNLVGEFNVAGDLWEIKRLLEKMGVK
VVASITGDSTVAEISAAHKAQLNLVQCSKSSGYIAEEMEKRHGTPHINVNFFGMRETMDSLISISEFFGLELAEKWVLGM
VEETKRKVARYRNTLEGKKVAIYVGGNKAWSLIRAFEDLGMEVIMSGTQNGLPNDYLRIMEKSGRELILFDDANPVELSR
LLNKYRPDLFVSGAKEKYLSYKLGIPFCEFNHDRIKPFSCFRGFLNFAEEVDKAVNSRVWKLAAPRTFLNCGVEYGYE
>Mature_478_residues
MNHSTGNSSNNKEFPGSNLEFQVGHVDRVIDTIPSRRGHFKRSGGGGPCCNKASVPGMVTQRSCVYGGARVVLMPITDAA
HIVHGPVGCAACTWDIRGSRSSKSDLYKTGFSTNLEEKDVVFGGEDRLLKCILEVDEIYSPSAIFVYSTCVSGVIGDDIT
AVCRNAEYKTGKWVIPVQSEGFRSFNKSLGHQLACDVLLDHVIGRGDAEVGERSVNLVGEFNVAGDLWEIKRLLEKMGVK
VVASITGDSTVAEISAAHKAQLNLVQCSKSSGYIAEEMEKRHGTPHINVNFFGMRETMDSLISISEFFGLELAEKWVLGM
VEETKRKVARYRNTLEGKKVAIYVGGNKAWSLIRAFEDLGMEVIMSGTQNGLPNDYLRIMEKSGRELILFDDANPVELSR
LLNKYRPDLFVSGAKEKYLSYKLGIPFCEFNHDRIKPFSCFRGFLNFAEEVDKAVNSRVWKLAAPRTFLNCGVEYGYE

Specific function: This protein may play a role in the biosynthesis of the prosthetic group of nitrogenase (FeMo cofactor)

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NIFE_METTH (O27607)

Other databases:

- EMBL:   AE000666
- PIR:   H69075
- RefSeq:   NP_276678.1
- ProteinModelPortal:   O27607
- SMR:   O27607
- STRING:   O27607
- GeneID:   1471834
- GenomeReviews:   AE000666_GR
- KEGG:   mth:MTH1565
- NMPDR:   fig|187420.1.peg.1533
- eggNOG:   arNOG04894
- HOGENOM:   HBG392742
- OMA:   CAFYGQN
- ProtClustDB:   CLSK878516
- BioCyc:   MTHE187420:MTH1565-MONOMER
- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005973
- TIGRFAMs:   TIGR01283

Pfam domain/function: PF00148 Oxidored_nitro

EC number: NA

Molecular weight: Translated: 52910; Mature: 52910

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS00699 NITROGENASE_1_1; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHSTGNSSNNKEFPGSNLEFQVGHVDRVIDTIPSRRGHFKRSGGGGPCCNKASVPGMVT
CCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
QRSCVYGGARVVLMPITDAAHIVHGPVGCAACTWDIRGSRSSKSDLYKTGFSTNLEEKDV
CCHHEECCCEEEEEECCCCHHHHCCCCCCEEEEEECCCCCCCCHHHHHCCCCCCCCCCCE
VFGGEDRLLKCILEVDEIYSPSAIFVYSTCVSGVIGDDITAVCRNAEYKTGKWVIPVQSE
EECCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHCCCCHHHHHCCCCCCCCCEEEEECCC
GFRSFNKSLGHQLACDVLLDHVIGRGDAEVGERSVNLVGEFNVAGDLWEIKRLLEKMGVK
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEEEEEECCCCCHHHHHHHHHHCCCE
VVASITGDSTVAEISAAHKAQLNLVQCSKSSGYIAEEMEKRHGTPHINVNFFGMRETMDS
EEEEECCCCHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHCCCCEEEEEEEEHHHHHHH
LISISEFFGLELAEKWVLGMVEETKRKVARYRNTLEGKKVAIYVGGNKAWSLIRAFEDLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHCC
MEVIMSGTQNGLPNDYLRIMEKSGRELILFDDANPVELSRLLNKYRPDLFVSGAKEKYLS
HHEEECCCCCCCCHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCEEECCCHHHHEE
YKLGIPFCEFNHDRIKPFSCFRGFLNFAEEVDKAVNSRVWKLAAPRTFLNCGVEYGYE
EECCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEECCHHHHHCCCCCCCC
>Mature Secondary Structure
MNHSTGNSSNNKEFPGSNLEFQVGHVDRVIDTIPSRRGHFKRSGGGGPCCNKASVPGMVT
CCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
QRSCVYGGARVVLMPITDAAHIVHGPVGCAACTWDIRGSRSSKSDLYKTGFSTNLEEKDV
CCHHEECCCEEEEEECCCCHHHHCCCCCCEEEEEECCCCCCCCHHHHHCCCCCCCCCCCE
VFGGEDRLLKCILEVDEIYSPSAIFVYSTCVSGVIGDDITAVCRNAEYKTGKWVIPVQSE
EECCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHCCCCHHHHHCCCCCCCCCEEEEECCC
GFRSFNKSLGHQLACDVLLDHVIGRGDAEVGERSVNLVGEFNVAGDLWEIKRLLEKMGVK
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEEEEEECCCCCHHHHHHHHHHCCCE
VVASITGDSTVAEISAAHKAQLNLVQCSKSSGYIAEEMEKRHGTPHINVNFFGMRETMDS
EEEEECCCCHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHCCCCEEEEEEEEHHHHHHH
LISISEFFGLELAEKWVLGMVEETKRKVARYRNTLEGKKVAIYVGGNKAWSLIRAFEDLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHCC
MEVIMSGTQNGLPNDYLRIMEKSGRELILFDDANPVELSRLLNKYRPDLFVSGAKEKYLS
HHEEECCCCCCCCHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCEEECCCHHHHEE
YKLGIPFCEFNHDRIKPFSCFRGFLNFAEEVDKAVNSRVWKLAAPRTFLNCGVEYGYE
EECCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEECCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463