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Identifier: 15679562

GI number: 15679562

Start: 1432624

End: 1433970

Strand: Direct

Name: Not Available

Synonym: MTH1566

Alternate gene names: 15679562

Gene position: 1432624-1433970 (Clockwise)

Preceding gene: 15679561

Following gene: 15679563

Centisome position: 81.8

GC content: 45.14

Gene sequence:

>1347_bases
ATGAATAGAAACATAACAATAAACCCCGGAAGGATCTGCCAGCCACTTGGCGCCGTCATGGCTTTCCTTGGAGTCAGGGG
AGCGATGCCACTAATACATGGTTCGCAGGGATGCAGTACATATATGAGGTTTCAGATAACGAGGCATTTCAGGGAACCAG
TTAACATTGCATCAAGCTCATTGAACGAGGCAACGGTTGTATACGGTGGAGAAAAAAACCTTCTGAAGGCTATAAGAACG
GTTGAGGAAAATTACAAACCGGAGGTCATAGGTGTTATCTCAGGTTGCCTTTCAGAGACCATAGGGGACGACATCAACAG
GATTGTGAGGATCTACAGGGATGCGGGATCGGAATCCCGAATAATTCCGGTTTCAACACCTGGCTTCAAAGGGTCCCATG
TGGATGGCTATGACATGGCAGTCACCTCCATCCTCAGATCCATCGCAGGGGATCCCTCAGATCAGATAGAGAGGGTTAAC
GTTATAAATGGCATCATGAGCCCAGCCGACACATATGAGTTAAAGAGTATTCTTAGGAGTATGGGAACGGAATTCCTCAT
GATAACTGATACCTCAGAATCCCTTGATGAACCATTCAATGGAGATCCCTACTTCATAACGCGGCACGGAACACCCATAA
GTAAAATAGAGGGGGCCTCTAACTCCATGGCAACACTAAGTCTTGGAGGATGTAAAGGCGGTCGGGTACTTGAAGGTAGA
TATGGGATTAAAAATATCGGTCTTGAGATCCCAGCAGGTCTTGAAAACACCGATCGTTTCATAGGTAAGTTAACGGAGCT
CTTTGATGCAAGTATTTCACAGTCCCTCATGAGGGACAGGGGACGCCTGATTGATGCCATGATTGATGCCCATCAGTATA
CCTATGGAAGGAATGTTGCAATCTTCACAGACCCTGGATATGCAGTGGCAATCACTTCCATGGTCCTTGAGATGGGAATG
ACACCTTCAGCCGTTTTTACAGGTTCAGAGAGCATATATTTCAATGAGAAAATTAGCCAACTTCAAAAGGAATACGGTTT
CAAGTGTATGGCCATGAGTGGCGCGGATATATTTGATTTACAGTCATATATTTCAGAAAAACCCATTGATGTTCTCATAG
GGAACTCTTACTGTGGTAGAGCGGCAGAAGAAAATGGAATACCCCTCATGAGGGTCGGCTTCCCGGTTTATGATAGACTT
GGTTCCCAGAGGATAAGAATTGCAGGTTACAGTGGAGGTATAGAATTTGTTGATTCCCTCACCAACCTGATAATTGAGAG
ATACTATGATGAGGGTTATGAAATATCCGATGTGAGTGACGCTGATGAGAGCTGTTGCATCAGATGA

Upstream 100 bases:

>100_bases
TTTTTGAATTTTGCAGAGGAAGTTGATAAGGCTGTAAACAGCAGAGTATGGAAACTGGCAGCACCAAGAACCTTTCTAAA
TTGCGGAGTTGAGTACGGTT

Downstream 100 bases:

>100_bases
TTTGAAATACGTTAAAATACCTGTAGATGGGGATACTCTTATAAAAATTGAATTTAATAAGGATATTCCTTAAATAAGGA
AATTTGATGGGATAAGACTG

Product: nitrogenase molybdenum-iron protein, NifN subunit

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MNRNITINPGRICQPLGAVMAFLGVRGAMPLIHGSQGCSTYMRFQITRHFREPVNIASSSLNEATVVYGGEKNLLKAIRT
VEENYKPEVIGVISGCLSETIGDDINRIVRIYRDAGSESRIIPVSTPGFKGSHVDGYDMAVTSILRSIAGDPSDQIERVN
VINGIMSPADTYELKSILRSMGTEFLMITDTSESLDEPFNGDPYFITRHGTPISKIEGASNSMATLSLGGCKGGRVLEGR
YGIKNIGLEIPAGLENTDRFIGKLTELFDASISQSLMRDRGRLIDAMIDAHQYTYGRNVAIFTDPGYAVAITSMVLEMGM
TPSAVFTGSESIYFNEKISQLQKEYGFKCMAMSGADIFDLQSYISEKPIDVLIGNSYCGRAAEENGIPLMRVGFPVYDRL
GSQRIRIAGYSGGIEFVDSLTNLIIERYYDEGYEISDVSDADESCCIR

Sequences:

>Translated_448_residues
MNRNITINPGRICQPLGAVMAFLGVRGAMPLIHGSQGCSTYMRFQITRHFREPVNIASSSLNEATVVYGGEKNLLKAIRT
VEENYKPEVIGVISGCLSETIGDDINRIVRIYRDAGSESRIIPVSTPGFKGSHVDGYDMAVTSILRSIAGDPSDQIERVN
VINGIMSPADTYELKSILRSMGTEFLMITDTSESLDEPFNGDPYFITRHGTPISKIEGASNSMATLSLGGCKGGRVLEGR
YGIKNIGLEIPAGLENTDRFIGKLTELFDASISQSLMRDRGRLIDAMIDAHQYTYGRNVAIFTDPGYAVAITSMVLEMGM
TPSAVFTGSESIYFNEKISQLQKEYGFKCMAMSGADIFDLQSYISEKPIDVLIGNSYCGRAAEENGIPLMRVGFPVYDRL
GSQRIRIAGYSGGIEFVDSLTNLIIERYYDEGYEISDVSDADESCCIR
>Mature_448_residues
MNRNITINPGRICQPLGAVMAFLGVRGAMPLIHGSQGCSTYMRFQITRHFREPVNIASSSLNEATVVYGGEKNLLKAIRT
VEENYKPEVIGVISGCLSETIGDDINRIVRIYRDAGSESRIIPVSTPGFKGSHVDGYDMAVTSILRSIAGDPSDQIERVN
VINGIMSPADTYELKSILRSMGTEFLMITDTSESLDEPFNGDPYFITRHGTPISKIEGASNSMATLSLGGCKGGRVLEGR
YGIKNIGLEIPAGLENTDRFIGKLTELFDASISQSLMRDRGRLIDAMIDAHQYTYGRNVAIFTDPGYAVAITSMVLEMGM
TPSAVFTGSESIYFNEKISQLQKEYGFKCMAMSGADIFDLQSYISEKPIDVLIGNSYCGRAAEENGIPLMRVGFPVYDRL
GSQRIRIAGYSGGIEFVDSLTNLIIERYYDEGYEISDVSDADESCCIR

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 49098; Mature: 49098

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00699 NITROGENASE_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRNITINPGRICQPLGAVMAFLGVRGAMPLIHGSQGCSTYMRFQITRHFREPVNIASSS
CCCCEECCCCHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHCC
LNEATVVYGGEKNLLKAIRTVEENYKPEVIGVISGCLSETIGDDINRIVRIYRDAGSESR
CCCEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
IIPVSTPGFKGSHVDGYDMAVTSILRSIAGDPSDQIERVNVINGIMSPADTYELKSILRS
EEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
MGTEFLMITDTSESLDEPFNGDPYFITRHGTPISKIEGASNSMATLSLGGCKGGRVLEGR
CCCCEEEEECCHHHHCCCCCCCCEEEEECCCCHHHHCCCCCCEEEEEECCCCCCEEEECC
YGIKNIGLEIPAGLENTDRFIGKLTELFDASISQSLMRDRGRLIDAMIDAHQYTYGRNVA
CCHHHCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
IFTDPGYAVAITSMVLEMGMTPSAVFTGSESIYFNEKISQLQKEYGFKCMAMSGADIFDL
EEECCCHHHHHHHHHHHHCCCCCEEEECCCCEEEHHHHHHHHHHHCCEEEEECCCCHHHH
QSYISEKPIDVLIGNSYCGRAAEENGIPLMRVGFPVYDRLGSQRIRIAGYSGGIEFVDSL
HHHHCCCCCEEEECCCCCCCCCCCCCCEEEEECCHHHHHCCCCEEEEEECCCCHHHHHHH
TNLIIERYYDEGYEISDVSDADESCCIR
HHHHHHHHHCCCCCCCCCCCCCHHCCCC
>Mature Secondary Structure
MNRNITINPGRICQPLGAVMAFLGVRGAMPLIHGSQGCSTYMRFQITRHFREPVNIASSS
CCCCEECCCCHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHCC
LNEATVVYGGEKNLLKAIRTVEENYKPEVIGVISGCLSETIGDDINRIVRIYRDAGSESR
CCCEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
IIPVSTPGFKGSHVDGYDMAVTSILRSIAGDPSDQIERVNVINGIMSPADTYELKSILRS
EEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
MGTEFLMITDTSESLDEPFNGDPYFITRHGTPISKIEGASNSMATLSLGGCKGGRVLEGR
CCCCEEEEECCHHHHCCCCCCCCEEEEECCCCHHHHCCCCCCEEEEEECCCCCCEEEECC
YGIKNIGLEIPAGLENTDRFIGKLTELFDASISQSLMRDRGRLIDAMIDAHQYTYGRNVA
CCHHHCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
IFTDPGYAVAITSMVLEMGMTPSAVFTGSESIYFNEKISQLQKEYGFKCMAMSGADIFDL
EEECCCHHHHHHHHHHHHCCCCCEEEECCCCEEEHHHHHHHHHHHCCEEEEECCCCHHHH
QSYISEKPIDVLIGNSYCGRAAEENGIPLMRVGFPVYDRLGSQRIRIAGYSGGIEFVDSL
HHHHCCCCCEEEECCCCCCCCCCCCCCEEEEECCHHHHHCCCCEEEEEECCCCHHHHHHH
TNLIIERYYDEGYEISDVSDADESCCIR
HHHHHHHHHCCCCCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8990309 [H]