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Identifier: 15679560

GI number: 15679560

Start: 1429847

End: 1431226

Strand: Direct

Name: Not Available

Synonym: MTH1564

Alternate gene names: 15679560

Gene position: 1429847-1431226 (Clockwise)

Preceding gene: 15679559

Following gene: 15679561

Centisome position: 81.64

GC content: 44.35

Gene sequence:

>1380_bases
ATGTCAGAAATAAATGTGATGGAAAGAACAAGGGAACTTGTGGTAAACCCTCTTGTGACCTGTCAGCCATTCGGGGCAAT
GTTCGCAACCCTTGGCATACGGAGGGGTCTCCCCATAGTCCATGGTTCGCAGGGTTGCAGCACCTTTGTGAGGTATGGTC
TTAATAGGCACTTCCGTGAACCGGCTGAAATAGCTGTCACATCGCTTCATGAGGATGCTGCGGTTTTTGGAGGCAGGAGC
AACCTCATAGATGGTGTTAAAAATCTTGTGAAGAGATTCAGGCCAGAACTCGTAGGAATTGTAACGACCTGTTCAAGTGA
GATAACTGGTGACGATGTGGATGGATTCCTGAGGGTTGCGGAGGCTGAATTAAGAGAAGAACTTGGAGAAAGATTCAGAA
CAAGGATGGTTAGCATATCAACACCAAGCTTTGTTGAACATCACTTAAGAGGATATGGAAATGCCCTAAAATCGTTTATA
GATACTCTTGCAGTTGATGAGGGGGACTGCAATGAGAGGATCAACCTGATACCAGGTATTGTTAACCCGGGTGATATACG
GGAGATAAGGCACATCTTTGAGTTGATGGACGTCGACCCCATTATACTCACAGACACTTCGGATCCATTCGATTCACCAC
TACGACCATCAAAGACAGAGAAAATGCCATTCTATCCTAAGGGTGGAACAGTGGTTTCTGATATAGAGGAATCCTCAAAC
AGTATAGGAACACTTTCAATGTCAATGTATGGAAATGAAGCATCAGAAACACTTAAAAAGCGTTTCAATATTCCCAAGGA
GCACCACATACCCATTGGTGTCAGGAATACAGACGATTTCGTCCGTTCATTGGCGAGAATAGCAGAAGTAGATGTGAGCG
AAGAGCTTCTGGATGAAAGAGGTATCCTCATTGATTCAATGGCAGATATTAGCTCAAGATATCTCTTTGGAAGGACAGCA
GCTGTTTACGGGGATCCCGACATGGTAATGGGTGTGTCAAGGTTCCTCTGTGAACTTGGAATCACACCACTCTATGCATG
TGTCGGTGTCGATAATGAAATCTTCAGGGAGGGAATGAAAAGAGTGGCATCCGAGGCAGATGAAAGGATAAACGTCATGA
TAAACTCAGATCTGAGGGCACTTGAAAGAGAACTTACAGAGGAACCTGTGGACTTTATGATAGGGAATTCGGATGGAAGG
CTTATAGCAAGGGATCTTGGAATACCCCTTGTAAGGATGGGCTTTCCTGTATATGATAGAGTTGGGTATCACAGGATTCC
TGTTGTGGGTTACCGTGGATCTGTCAATCTTCTAAACAGAATAACAAACACAGTACTGAGAGAATACTATGAACCACAGC
ACTGGAAACTCCAGCAATAA

Upstream 100 bases:

>100_bases
TTACATAGGTTTTGAGGGGTTCCTGAATCTCGCAAGGGACATGTATGCATCAATATACAGTCCTGTCTGGGATCTCCTTG
AATTTGAGGCAGGTGATTGA

Downstream 100 bases:

>100_bases
TAAAGAATTTCCTGGAAGTAACCTTGAATTCCAGGTGGGGCATGTTGATAGGGTAATCGACACCATCCCCTCAAGGAGAG
GACACTTCAAAAGGAGTGGA

Product: nitrogenase molybdenum-iron protein, NifK subunit

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MSEINVMERTRELVVNPLVTCQPFGAMFATLGIRRGLPIVHGSQGCSTFVRYGLNRHFREPAEIAVTSLHEDAAVFGGRS
NLIDGVKNLVKRFRPELVGIVTTCSSEITGDDVDGFLRVAEAELREELGERFRTRMVSISTPSFVEHHLRGYGNALKSFI
DTLAVDEGDCNERINLIPGIVNPGDIREIRHIFELMDVDPIILTDTSDPFDSPLRPSKTEKMPFYPKGGTVVSDIEESSN
SIGTLSMSMYGNEASETLKKRFNIPKEHHIPIGVRNTDDFVRSLARIAEVDVSEELLDERGILIDSMADISSRYLFGRTA
AVYGDPDMVMGVSRFLCELGITPLYACVGVDNEIFREGMKRVASEADERINVMINSDLRALERELTEEPVDFMIGNSDGR
LIARDLGIPLVRMGFPVYDRVGYHRIPVVGYRGSVNLLNRITNTVLREYYEPQHWKLQQ

Sequences:

>Translated_459_residues
MSEINVMERTRELVVNPLVTCQPFGAMFATLGIRRGLPIVHGSQGCSTFVRYGLNRHFREPAEIAVTSLHEDAAVFGGRS
NLIDGVKNLVKRFRPELVGIVTTCSSEITGDDVDGFLRVAEAELREELGERFRTRMVSISTPSFVEHHLRGYGNALKSFI
DTLAVDEGDCNERINLIPGIVNPGDIREIRHIFELMDVDPIILTDTSDPFDSPLRPSKTEKMPFYPKGGTVVSDIEESSN
SIGTLSMSMYGNEASETLKKRFNIPKEHHIPIGVRNTDDFVRSLARIAEVDVSEELLDERGILIDSMADISSRYLFGRTA
AVYGDPDMVMGVSRFLCELGITPLYACVGVDNEIFREGMKRVASEADERINVMINSDLRALERELTEEPVDFMIGNSDGR
LIARDLGIPLVRMGFPVYDRVGYHRIPVVGYRGSVNLLNRITNTVLREYYEPQHWKLQQ
>Mature_458_residues
SEINVMERTRELVVNPLVTCQPFGAMFATLGIRRGLPIVHGSQGCSTFVRYGLNRHFREPAEIAVTSLHEDAAVFGGRSN
LIDGVKNLVKRFRPELVGIVTTCSSEITGDDVDGFLRVAEAELREELGERFRTRMVSISTPSFVEHHLRGYGNALKSFID
TLAVDEGDCNERINLIPGIVNPGDIREIRHIFELMDVDPIILTDTSDPFDSPLRPSKTEKMPFYPKGGTVVSDIEESSNS
IGTLSMSMYGNEASETLKKRFNIPKEHHIPIGVRNTDDFVRSLARIAEVDVSEELLDERGILIDSMADISSRYLFGRTAA
VYGDPDMVMGVSRFLCELGITPLYACVGVDNEIFREGMKRVASEADERINVMINSDLRALERELTEEPVDFMIGNSDGRL
IARDLGIPLVRMGFPVYDRVGYHRIPVVGYRGSVNLLNRITNTVLREYYEPQHWKLQQ

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NIFK_METTH (O27606)

Other databases:

- EMBL:   AE000666
- PIR:   G69075
- RefSeq:   NP_276677.1
- ProteinModelPortal:   O27606
- STRING:   O27606
- GeneID:   1471833
- GenomeReviews:   AE000666_GR
- KEGG:   mth:MTH1564
- NMPDR:   fig|187420.1.peg.1532
- eggNOG:   arNOG04893
- HOGENOM:   HBG336444
- OMA:   FEIESFD
- ProtClustDB:   CLSK878515
- BioCyc:   MTHE187420:MTH1564-MONOMER
- BRENDA:   1.18.6.1
- InterPro:   IPR000510
- InterPro:   IPR000318

Pfam domain/function: PF00148 Oxidored_nitro

EC number: =1.18.6.1

Molecular weight: Translated: 51490; Mature: 51359

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00699 NITROGENASE_1_1; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEINVMERTRELVVNPLVTCQPFGAMFATLGIRRGLPIVHGSQGCSTFVRYGLNRHFRE
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHCCC
PAEIAVTSLHEDAAVFGGRSNLIDGVKNLVKRFRPELVGIVTTCSSEITGDDVDGFLRVA
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCHHHHHHHH
EAELREELGERFRTRMVSISTPSFVEHHLRGYGNALKSFIDTLAVDEGDCNERINLIPGI
HHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCC
VNPGDIREIRHIFELMDVDPIILTDTSDPFDSPLRPSKTEKMPFYPKGGTVVSDIEESSN
CCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCC
SIGTLSMSMYGNEASETLKKRFNIPKEHHIPIGVRNTDDFVRSLARIAEVDVSEELLDER
CCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHC
GILIDSMADISSRYLFGRTAAVYGDPDMVMGVSRFLCELGITPLYACVGVDNEIFREGMK
CCEEEHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHH
RVASEADERINVMINSDLRALERELTEEPVDFMIGNSDGRLIARDLGIPLVRMGFPVYDR
HHHHHHCCCEEEEECCHHHHHHHHHHCCCCCEEEECCCCCEEEHHCCCCHHHHCCCHHHH
VGYHRIPVVGYRGSVNLLNRITNTVLREYYEPQHWKLQQ
CCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SEINVMERTRELVVNPLVTCQPFGAMFATLGIRRGLPIVHGSQGCSTFVRYGLNRHFRE
CCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHCCC
PAEIAVTSLHEDAAVFGGRSNLIDGVKNLVKRFRPELVGIVTTCSSEITGDDVDGFLRVA
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCHHHHHHHH
EAELREELGERFRTRMVSISTPSFVEHHLRGYGNALKSFIDTLAVDEGDCNERINLIPGI
HHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCC
VNPGDIREIRHIFELMDVDPIILTDTSDPFDSPLRPSKTEKMPFYPKGGTVVSDIEESSN
CCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCC
SIGTLSMSMYGNEASETLKKRFNIPKEHHIPIGVRNTDDFVRSLARIAEVDVSEELLDER
CCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHC
GILIDSMADISSRYLFGRTAAVYGDPDMVMGVSRFLCELGITPLYACVGVDNEIFREGMK
CCEEEHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHH
RVASEADERINVMINSDLRALERELTEEPVDFMIGNSDGRLIARDLGIPLVRMGFPVYDR
HHHHHHCCCEEEEECCHHHHHHHHHHCCCCCEEEECCCCCEEEHHCCCCHHHHCCCHHHH
VGYHRIPVVGYRGSVNLLNRITNTVLREYYEPQHWKLQQ
CCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463