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The map label for this gene is leuD

Identifier: 15679386

GI number: 15679386

Start: 1255181

End: 1255669

Strand: Direct

Name: leuD

Synonym: MTH1387

Alternate gene names: 15679386

Gene position: 1255181-1255669 (Clockwise)

Preceding gene: 15679385

Following gene: 15679387

Centisome position: 71.67

GC content: 51.74

Gene sequence:

>489_bases
ATGAAAGGAAAGGTTTGGAAGTTCCCGGACGACGTGGACACTGACATAATCATACCCGGAAGGTACCTTGTAATGAGGGA
CCCTGAGAAGCTGAGGGAACACGTGATGGAGGGTCTGGACCCTGAATTCCCGTCAAAGGTGAAACCCGGTGACTTCATCG
TCGCCGGAAAAAACTTCGGGTGCGGTTCATCCAGGGAGCACGCCCCCCTGGCCCTGAAGGGTGCCGGGATAGCCGCGGTA
ATCGCAGAGTCCTTTGCAAGGATATTCTACAGGAACGCCATAAACGTTGGAATACCCCTCCTTGAGGCCCCGGGGATAAC
AGAGAAACTCAATGAGGGTGATGAGATAGAGGTGGACCTCGATAGGGGTGTTATAATAAGGGGAGATGATGAATTCCCCT
TCAAGAAACTCCCGGACTTCATGGTTGAGATACTCGAGAAGGGTGGTCTTATACCCTACCTCAAGAAAAAGGGAGACTTT
AAAGGGTGA

Upstream 100 bases:

>100_bases
CCGAAGCAGAGGTCTACCTCTCCTCAGCAGCCGTGGCAGCCGCATCTGCAGTGAAGGGCAGCATAACCCACCCTGGGAGT
CTGAAGTAGAGGTGATGGTA

Downstream 100 bases:

>100_bases
AATCAATGAAGATAGCAGTCATACCCGGTGATGGAATCGGAGTGGAGGTGATGGAGGCAGCCCTCCACATCCTGAACACC
CTCGACCTTGACCTTGAATT

Product: 3-isopropylmalate dehydratase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 2; IPMI 2; Isopropylmalate isomerase 2

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MKGKVWKFPDDVDTDIIIPGRYLVMRDPEKLREHVMEGLDPEFPSKVKPGDFIVAGKNFGCGSSREHAPLALKGAGIAAV
IAESFARIFYRNAINVGIPLLEAPGITEKLNEGDEIEVDLDRGVIIRGDDEFPFKKLPDFMVEILEKGGLIPYLKKKGDF
KG

Sequences:

>Translated_162_residues
MKGKVWKFPDDVDTDIIIPGRYLVMRDPEKLREHVMEGLDPEFPSKVKPGDFIVAGKNFGCGSSREHAPLALKGAGIAAV
IAESFARIFYRNAINVGIPLLEAPGITEKLNEGDEIEVDLDRGVIIRGDDEFPFKKLPDFMVEILEKGGLIPYLKKKGDF
KG
>Mature_162_residues
MKGKVWKFPDDVDTDIIIPGRYLVMRDPEKLREHVMEGLDPEFPSKVKPGDFIVAGKNFGCGSSREHAPLALKGAGIAAV
IAESFARIFYRNAINVGIPLLEAPGITEKLNEGDEIEVDLDRGVIIRGDDEFPFKKLPDFMVEILEKGGLIPYLKKKGDF
KG

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=145, Percent_Identity=35.1724137931034, Blast_Score=65, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320440, Length=105, Percent_Identity=47.6190476190476, Blast_Score=96, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6321429, Length=155, Percent_Identity=38.0645161290323, Blast_Score=71, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD2_METTH (O27440)

Other databases:

- EMBL:   AE000666
- PIR:   E69051
- RefSeq:   NP_276503.1
- ProteinModelPortal:   O27440
- SMR:   O27440
- STRING:   O27440
- GeneID:   1471104
- GenomeReviews:   AE000666_GR
- KEGG:   mth:MTH1387
- NMPDR:   fig|187420.1.peg.1358
- eggNOG:   arNOG09974
- HOGENOM:   HBG304838
- OMA:   PFPEFMQ
- ProtClustDB:   PRK00439
- BioCyc:   MTHE187420:MTH1387-MONOMER
- BRENDA:   4.2.1.33
- HAMAP:   MF_01032
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR011827
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR02087

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 17956; Mature: 17956

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGKVWKFPDDVDTDIIIPGRYLVMRDPEKLREHVMEGLDPEFPSKVKPGDFIVAGKNFG
CCCCCCCCCCCCCCCEEECCEEEEEECHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCC
CGSSREHAPLALKGAGIAAVIAESFARIFYRNAINVGIPLLEAPGITEKLNEGDEIEVDL
CCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHCCCCCEEEEEC
DRGVIIRGDDEFPFKKLPDFMVEILEKGGLIPYLKKKGDFKG
CCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEHHCCCCCCC
>Mature Secondary Structure
MKGKVWKFPDDVDTDIIIPGRYLVMRDPEKLREHVMEGLDPEFPSKVKPGDFIVAGKNFG
CCCCCCCCCCCCCCCEEECCEEEEEECHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCC
CGSSREHAPLALKGAGIAAVIAESFARIFYRNAINVGIPLLEAPGITEKLNEGDEIEVDL
CCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHCCCCCEEEEEC
DRGVIIRGDDEFPFKKLPDFMVEILEKGGLIPYLKKKGDFKG
CCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463