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Identifier: 15679387

GI number: 15679387

Start: 1255666

End: 1256655

Strand: Direct

Name: Not Available

Synonym: MTH1388

Alternate gene names: 15679387

Gene position: 1255666-1256655 (Clockwise)

Preceding gene: 15679386

Following gene: 15679388

Centisome position: 71.7

GC content: 52.02

Gene sequence:

>990_bases
GTGAAATCAATGAAGATAGCAGTCATACCCGGTGATGGAATCGGAGTGGAGGTGATGGAGGCAGCCCTCCACATCCTGAA
CACCCTCGACCTTGACCTTGAATTCATACATGCAGATGCCGGTGATGCCTGCCTTAAAAGGACAGGGACGGCACTGCCAG
AGGAGACCCTTGAAGCCGTGGGTGAAGCCAGGGCAACCCTCTTCGGGGCTGCGGGTGAAAGCGCAGCTGACGTTATAGTC
CGCCTGAGGCGTGAATTTGATCTTTTTGCGAATTTGAGGCCTGTTAAGTCCCTGCCAGGTGTCCCCTGCCTCTACCCGGA
CCTTGACTTTGTGATTGTGCGGGAGAACACCGAGGACCTCTATGTCGGTGACGAGGAGTACACCCCTGAGGGCGCCGTTG
CAAAAAGAATCATAACAAGAACCGCCTCCAGGAGAATCAGCCAATTCGCCTTCCAGTACGCCCAGAAAGAGGGCATGCAA
AAGGTAACAGCAGTCCACAAGGCCAACGTACTCAAAAAAACCGACGGAATCTTCAGAGACGAATTCTATAAAGTGGCATC
AGAATACCCCCAGATGGAGGCCACCGACTACTACGTGGACGCCACAGCAATGTACCTCATAACCCAGCCCCAGGAATTCC
AGACCATCGTCACAACCAACCTATTCGGAGACATACTATCAGACGAAGCCGCAGGACTCATCGGAGGACTCGGACTCGCA
CCATCAGCAAACATAGGAGAAAAAAACGCACTATTCGAACCAGTGCACGGATCAGCACCCCAAATCGCAGGAAAAAACAT
AGCCAACCCCACAGCCATGATACTAACAACCACACTAATGCTAAAACACCTCAACAAAAAACAAGAAGCCCAAAAAATAG
AAAAAGCACTCCAAAAAACCCTTGCAGAGGGTCTTGTGACACCGGACCTCGGTGGGAAGCTTGGCACCATGGAGATGGCT
GCCGAGATAGCCAGACACCTGGAGGATTGA

Upstream 100 bases:

>100_bases
ATGATGAATTCCCCTTCAAGAAACTCCCGGACTTCATGGTTGAGATACTCGAGAAGGGTGGTCTTATACCCTACCTCAAG
AAAAAGGGAGACTTTAAAGG

Downstream 100 bases:

>100_bases
TATTCATGCGTACAGAGGACGTCAGGAGAGACATACCCCTCCTCAGGGATCATGTCTACCTCGACGCTGCCAGCACAACC
CCCACTCCACTCCCGGTTGT

Product: multifunctional 3-isopropylmalate dehydrogenase/D-malate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; IMDH; Beta-IPM dehydrogenase

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MKSMKIAVIPGDGIGVEVMEAALHILNTLDLDLEFIHADAGDACLKRTGTALPEETLEAVGEARATLFGAAGESAADVIV
RLRREFDLFANLRPVKSLPGVPCLYPDLDFVIVRENTEDLYVGDEEYTPEGAVAKRIITRTASRRISQFAFQYAQKEGMQ
KVTAVHKANVLKKTDGIFRDEFYKVASEYPQMEATDYYVDATAMYLITQPQEFQTIVTTNLFGDILSDEAAGLIGGLGLA
PSANIGEKNALFEPVHGSAPQIAGKNIANPTAMILTTTLMLKHLNKKQEAQKIEKALQKTLAEGLVTPDLGGKLGTMEMA
AEIARHLED

Sequences:

>Translated_329_residues
MKSMKIAVIPGDGIGVEVMEAALHILNTLDLDLEFIHADAGDACLKRTGTALPEETLEAVGEARATLFGAAGESAADVIV
RLRREFDLFANLRPVKSLPGVPCLYPDLDFVIVRENTEDLYVGDEEYTPEGAVAKRIITRTASRRISQFAFQYAQKEGMQ
KVTAVHKANVLKKTDGIFRDEFYKVASEYPQMEATDYYVDATAMYLITQPQEFQTIVTTNLFGDILSDEAAGLIGGLGLA
PSANIGEKNALFEPVHGSAPQIAGKNIANPTAMILTTTLMLKHLNKKQEAQKIEKALQKTLAEGLVTPDLGGKLGTMEMA
AEIARHLED
>Mature_329_residues
MKSMKIAVIPGDGIGVEVMEAALHILNTLDLDLEFIHADAGDACLKRTGTALPEETLEAVGEARATLFGAAGESAADVIV
RLRREFDLFANLRPVKSLPGVPCLYPDLDFVIVRENTEDLYVGDEEYTPEGAVAKRIITRTASRRISQFAFQYAQKEGMQ
KVTAVHKANVLKKTDGIFRDEFYKVASEYPQMEATDYYVDATAMYLITQPQEFQTIVTTNLFGDILSDEAAGLIGGLGLA
PSANIGEKNALFEPVHGSAPQIAGKNIANPTAMILTTTLMLKHLNKKQEAQKIEKALQKTLAEGLVTPDLGGKLGTMEMA
AEIARHLED

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family

Homologues:

Organism=Homo sapiens, GI5031777, Length=333, Percent_Identity=38.4384384384384, Blast_Score=235, Evalue=4e-62,
Organism=Homo sapiens, GI4758582, Length=333, Percent_Identity=38.1381381381381, Blast_Score=212, Evalue=3e-55,
Organism=Homo sapiens, GI28178821, Length=336, Percent_Identity=36.9047619047619, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI28178816, Length=320, Percent_Identity=37.8125, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI28178838, Length=305, Percent_Identity=38.3606557377049, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI28178819, Length=195, Percent_Identity=40.5128205128205, Blast_Score=142, Evalue=5e-34,
Organism=Escherichia coli, GI87081683, Length=359, Percent_Identity=37.883008356546, Blast_Score=212, Evalue=3e-56,
Organism=Escherichia coli, GI1788101, Length=357, Percent_Identity=35.0140056022409, Blast_Score=204, Evalue=7e-54,
Organism=Escherichia coli, GI1787381, Length=366, Percent_Identity=31.1475409836066, Blast_Score=164, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI71986051, Length=331, Percent_Identity=38.0664652567976, Blast_Score=240, Evalue=8e-64,
Organism=Caenorhabditis elegans, GI17550882, Length=332, Percent_Identity=37.9518072289157, Blast_Score=207, Evalue=6e-54,
Organism=Caenorhabditis elegans, GI25144293, Length=349, Percent_Identity=35.8166189111748, Blast_Score=190, Evalue=8e-49,
Organism=Caenorhabditis elegans, GI17505779, Length=329, Percent_Identity=35.258358662614, Blast_Score=179, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6322097, Length=352, Percent_Identity=43.4659090909091, Blast_Score=247, Evalue=2e-66,
Organism=Saccharomyces cerevisiae, GI6324291, Length=338, Percent_Identity=37.5739644970414, Blast_Score=209, Evalue=5e-55,
Organism=Saccharomyces cerevisiae, GI6324709, Length=332, Percent_Identity=35.5421686746988, Blast_Score=206, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6319830, Length=356, Percent_Identity=34.2696629213483, Blast_Score=169, Evalue=4e-43,
Organism=Drosophila melanogaster, GI24643268, Length=329, Percent_Identity=39.8176291793313, Blast_Score=254, Evalue=7e-68,
Organism=Drosophila melanogaster, GI24643270, Length=329, Percent_Identity=39.8176291793313, Blast_Score=253, Evalue=9e-68,
Organism=Drosophila melanogaster, GI24661184, Length=335, Percent_Identity=38.8059701492537, Blast_Score=229, Evalue=3e-60,
Organism=Drosophila melanogaster, GI161078637, Length=337, Percent_Identity=32.6409495548961, Blast_Score=192, Evalue=2e-49,
Organism=Drosophila melanogaster, GI161078635, Length=337, Percent_Identity=32.6409495548961, Blast_Score=192, Evalue=2e-49,
Organism=Drosophila melanogaster, GI161078633, Length=337, Percent_Identity=32.6409495548961, Blast_Score=192, Evalue=3e-49,
Organism=Drosophila melanogaster, GI24650122, Length=337, Percent_Identity=32.6409495548961, Blast_Score=192, Evalue=3e-49,
Organism=Drosophila melanogaster, GI161078639, Length=335, Percent_Identity=32.8358208955224, Blast_Score=192, Evalue=4e-49,
Organism=Drosophila melanogaster, GI281362242, Length=328, Percent_Identity=34.1463414634146, Blast_Score=173, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24648872, Length=328, Percent_Identity=34.1463414634146, Blast_Score=173, Evalue=1e-43,
Organism=Drosophila melanogaster, GI20130355, Length=330, Percent_Identity=26.969696969697, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI45550436, Length=239, Percent_Identity=24.6861924686192, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU3_METTH (O27441)

Other databases:

- EMBL:   AE000666
- PIR:   F69051
- RefSeq:   NP_276504.1
- ProteinModelPortal:   O27441
- SMR:   O27441
- STRING:   O27441
- GeneID:   1471105
- GenomeReviews:   AE000666_GR
- KEGG:   mth:MTH1388
- NMPDR:   fig|187420.1.peg.1359
- eggNOG:   arNOG05472
- HOGENOM:   HBG518924
- OMA:   TCAHKAN
- PhylomeDB:   O27441
- ProtClustDB:   PRK14025
- BioCyc:   MTHE187420:MTH1388-MONOMER
- BRENDA:   1.1.1.85
- GO:   GO:0005737
- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011828
- Gene3D:   G3DSA:3.40.718.10
- PANTHER:   PTHR11835
- TIGRFAMs:   TIGR02088

Pfam domain/function: PF00180 Iso_dh

EC number: =1.1.1.85

Molecular weight: Translated: 35785; Mature: 35785

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00470 IDH_IMDH

Important sites: BINDING 83-83 BINDING 93-93 BINDING 114-114 BINDING 200-200

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSMKIAVIPGDGIGVEVMEAALHILNTLDLDLEFIHADAGDACLKRTGTALPEETLEAV
CCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHCCCCCCHHHHHHH
GEARATLFGAAGESAADVIVRLRREFDLFANLRPVKSLPGVPCLYPDLDFVIVRENTEDL
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCEECCCCCEEEEECCCCCE
YVGDEEYTPEGAVAKRIITRTASRRISQFAFQYAQKEGMQKVTAVHKANVLKKTDGIFRD
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
EFYKVASEYPQMEATDYYVDATAMYLITQPQEFQTIVTTNLFGDILSDEAAGLIGGLGLA
HHHHHHHHCCCCCCCEEEECCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PSANIGEKNALFEPVHGSAPQIAGKNIANPTAMILTTTLMLKHLNKKQEAQKIEKALQKT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
LAEGLVTPDLGGKLGTMEMAAEIARHLED
HHHCCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKSMKIAVIPGDGIGVEVMEAALHILNTLDLDLEFIHADAGDACLKRTGTALPEETLEAV
CCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHCCCCCCHHHHHHH
GEARATLFGAAGESAADVIVRLRREFDLFANLRPVKSLPGVPCLYPDLDFVIVRENTEDL
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCEECCCCCEEEEECCCCCE
YVGDEEYTPEGAVAKRIITRTASRRISQFAFQYAQKEGMQKVTAVHKANVLKKTDGIFRD
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
EFYKVASEYPQMEATDYYVDATAMYLITQPQEFQTIVTTNLFGDILSDEAAGLIGGLGLA
HHHHHHHHCCCCCCCEEEECCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PSANIGEKNALFEPVHGSAPQIAGKNIANPTAMILTTTLMLKHLNKKQEAQKIEKALQKT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
LAEGLVTPDLGGKLGTMEMAAEIARHLED
HHHCCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463