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Identifier: 15679385

GI number: 15679385

Start: 1253910

End: 1255169

Strand: Direct

Name: Not Available

Synonym: MTH1386

Alternate gene names: 15679385

Gene position: 1253910-1255169 (Clockwise)

Preceding gene: 15679376

Following gene: 15679386

Centisome position: 71.6

GC content: 54.6

Gene sequence:

>1260_bases
ATGTCTATGACTGTTTCTGAGAAGATACTTGCCAGGGCTTCAGGTAAGGATAGGGTGGAAGCCGGTGAAATAGTTATGGC
GGATATAGATGTTGCGATGACCCATGACCTCACAGGTCCCCTGTCGGTTGAGTCCTTCAGGGCCATAGGTGAGGATAGGG
TCTGGGACCCTGAAAAAATCGTTGTGATATTCGATCACCAGGTCCCTGCAGACTCCATTGAAGCCGCCCAGAACCACATG
ATAATGAGGGACTTCGTAGAGGAGCAGGGAATACGGAACTTCTATGACGTTCGTGAAGGCGTCTGTCACCAGGTGCTCCC
TGAGAAGGGCCATGTTGTGCCCGGTGAGGTCGTGGTGGGTACAGACTCCCACACCTGCACCCACGGGGCCCTCGGAGCCT
TCGCAACGGGTATAGGTTCAACAGATATGGCCATGGTATTTGCAACTGGGAAACTTTGGTTCAGGGTCCCTGAAACCCTG
AGATTTGATGTGAGGGGAAAACTCAGGGAGCACGTCTATGCAAAGGACGTCATACTGAACATAATAGGGAGGGTTGGTGC
CGATGGCGCAACCTACATGGCCTGTGAATTTGCAGGTGAAACCGTTGCAGAAATGAGTGTATCCGACCGTATGGTCCTCT
CAAATATGGCAATAGAGATGGGTGGTAAGACAGGAATCGTGGAACCTGACGAAAAAACCCTGAACTATGTCAGGAGGAGG
TCAGGTAAACCCTGGAGGGTCTTCAAAACAGACCCTGACGCTCCATCCCTCAGTGTCATGGAGGTCGACGTGAGTGACCT
TGAACCCCAGGTCGCCTGTCCACACAATGTGGACAATGTGAAACCCGTCACCGAGGTGGAGGGGACAGAGATAGACCAGG
TCTTCCTTGGTTCATGCACAAATGGAAGGCTCAGTGACCTCAGGGACGCTGCCGCCATACTGAAGAACAGGAAGGTCTCA
GACAGTGTCAGGATGCTCGTCATACCTGCCTCCAGGGAGGTCTACCGCAGGGCCCTTGACGAGGGACTGATTGAGATATT
CGTGGATGCAGGAGCCCTGGTATGCAACCCCTGCTGCGGCCCCTGCCTCGGAGGACACGTTGGACTGGTCGGACCAGGGG
AGGTGAGCCTCTCAACCTCAAACAGGAACTTCAGGGGGAGGCAGGGAAGCCCCGAAGCAGAGGTCTACCTCTCCTCAGCA
GCCGTGGCAGCCGCATCTGCAGTGAAGGGCAGCATAACCCACCCTGGGAGTCTGAAGTAG

Upstream 100 bases:

>100_bases
CAGAAGGTTAAAGAGGCTCTAATTTTCACAGACTGCCCCATGGACCACTTGCTGGAACCTTTATGAACCCTTCAATTATT
CTGCTAAAGGAGGATAGAAG

Downstream 100 bases:

>100_bases
AGGTGATGGTAATGAAAGGAAAGGTTTGGAAGTTCCCGGACGACGTGGACACTGACATAATCATACCCGGAAGGTACCTT
GTAATGAGGGACCCTGAGAA

Product: 3-isopropylmalate dehydratase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MSMTVSEKILARASGKDRVEAGEIVMADIDVAMTHDLTGPLSVESFRAIGEDRVWDPEKIVVIFDHQVPADSIEAAQNHM
IMRDFVEEQGIRNFYDVREGVCHQVLPEKGHVVPGEVVVGTDSHTCTHGALGAFATGIGSTDMAMVFATGKLWFRVPETL
RFDVRGKLREHVYAKDVILNIIGRVGADGATYMACEFAGETVAEMSVSDRMVLSNMAIEMGGKTGIVEPDEKTLNYVRRR
SGKPWRVFKTDPDAPSLSVMEVDVSDLEPQVACPHNVDNVKPVTEVEGTEIDQVFLGSCTNGRLSDLRDAAAILKNRKVS
DSVRMLVIPASREVYRRALDEGLIEIFVDAGALVCNPCCGPCLGGHVGLVGPGEVSLSTSNRNFRGRQGSPEAEVYLSSA
AVAAASAVKGSITHPGSLK

Sequences:

>Translated_419_residues
MSMTVSEKILARASGKDRVEAGEIVMADIDVAMTHDLTGPLSVESFRAIGEDRVWDPEKIVVIFDHQVPADSIEAAQNHM
IMRDFVEEQGIRNFYDVREGVCHQVLPEKGHVVPGEVVVGTDSHTCTHGALGAFATGIGSTDMAMVFATGKLWFRVPETL
RFDVRGKLREHVYAKDVILNIIGRVGADGATYMACEFAGETVAEMSVSDRMVLSNMAIEMGGKTGIVEPDEKTLNYVRRR
SGKPWRVFKTDPDAPSLSVMEVDVSDLEPQVACPHNVDNVKPVTEVEGTEIDQVFLGSCTNGRLSDLRDAAAILKNRKVS
DSVRMLVIPASREVYRRALDEGLIEIFVDAGALVCNPCCGPCLGGHVGLVGPGEVSLSTSNRNFRGRQGSPEAEVYLSSA
AVAAASAVKGSITHPGSLK
>Mature_418_residues
SMTVSEKILARASGKDRVEAGEIVMADIDVAMTHDLTGPLSVESFRAIGEDRVWDPEKIVVIFDHQVPADSIEAAQNHMI
MRDFVEEQGIRNFYDVREGVCHQVLPEKGHVVPGEVVVGTDSHTCTHGALGAFATGIGSTDMAMVFATGKLWFRVPETLR
FDVRGKLREHVYAKDVILNIIGRVGADGATYMACEFAGETVAEMSVSDRMVLSNMAIEMGGKTGIVEPDEKTLNYVRRRS
GKPWRVFKTDPDAPSLSVMEVDVSDLEPQVACPHNVDNVKPVTEVEGTEIDQVFLGSCTNGRLSDLRDAAAILKNRKVSD
SVRMLVIPASREVYRRALDEGLIEIFVDAGALVCNPCCGPCLGGHVGLVGPGEVSLSTSNRNFRGRQGSPEAEVYLSSAA
VAAASAVKGSITHPGSLK

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=446, Percent_Identity=26.2331838565022, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI8659555, Length=488, Percent_Identity=23.5655737704918, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1786259, Length=422, Percent_Identity=36.7298578199052, Blast_Score=236, Evalue=1e-63,
Organism=Escherichia coli, GI87081781, Length=426, Percent_Identity=23.2394366197183, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI2367097, Length=433, Percent_Identity=24.2494226327945, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1787531, Length=388, Percent_Identity=24.2268041237113, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI32564738, Length=342, Percent_Identity=28.0701754385965, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI25149337, Length=342, Percent_Identity=28.0701754385965, Blast_Score=114, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI25149342, Length=282, Percent_Identity=26.5957446808511, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17568399, Length=399, Percent_Identity=22.3057644110276, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6321429, Length=432, Percent_Identity=32.1759259259259, Blast_Score=219, Evalue=5e-58,
Organism=Saccharomyces cerevisiae, GI6320440, Length=425, Percent_Identity=31.2941176470588, Blast_Score=213, Evalue=4e-56,
Organism=Saccharomyces cerevisiae, GI6323335, Length=448, Percent_Identity=25.8928571428571, Blast_Score=139, Evalue=8e-34,
Organism=Saccharomyces cerevisiae, GI6322261, Length=455, Percent_Identity=25.9340659340659, Blast_Score=132, Evalue=7e-32,
Organism=Drosophila melanogaster, GI28571643, Length=448, Percent_Identity=25.8928571428571, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI281365315, Length=447, Percent_Identity=24.3847874720358, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI17864292, Length=447, Percent_Identity=24.3847874720358, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI161076999, Length=411, Percent_Identity=24.5742092457421, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24645686, Length=244, Percent_Identity=28.6885245901639, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI17137564, Length=173, Percent_Identity=31.7919075144509, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC1_METTH (O27439)

Other databases:

- EMBL:   AE000666
- PIR:   D69051
- RefSeq:   NP_276502.1
- ProteinModelPortal:   O27439
- STRING:   O27439
- GeneID:   1471103
- GenomeReviews:   AE000666_GR
- KEGG:   mth:MTH1386
- NMPDR:   fig|187420.1.peg.1357
- eggNOG:   arNOG04337
- HOGENOM:   HBG330745
- OMA:   TDPRDFL
- PhylomeDB:   O27439
- ProtClustDB:   PRK00402
- BioCyc:   MTHE187420:MTH1386-MONOMER
- BRENDA:   4.2.1.33
- HAMAP:   MF_01027
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR01343
- TIGRFAMs:   TIGR02086

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 45198; Mature: 45067

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMTVSEKILARASGKDRVEAGEIVMADIDVAMTHDLTGPLSVESFRAIGEDRVWDPEKI
CCCCHHHHHHHHCCCCCCCCCCCEEEEECCEEEECCCCCCCCHHHHHHCCCCCCCCCCEE
VVIFDHQVPADSIEAAQNHMIMRDFVEEQGIRNFYDVREGVCHQVLPEKGHVVPGEVVVG
EEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
TDSHTCTHGALGAFATGIGSTDMAMVFATGKLWFRVPETLRFDVRGKLREHVYAKDVILN
CCCCCCCCCCHHHHHHCCCCCCEEEEEEECEEEEECCCCEEECCHHHHHHHHHHHHHHHH
IIGRVGADGATYMACEFAGETVAEMSVSDRMVLSNMAIEMGGKTGIVEPDEKTLNYVRRR
HHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHC
SGKPWRVFKTDPDAPSLSVMEVDVSDLEPQVACPHNVDNVKPVTEVEGTEIDQVFLGSCT
CCCCEEEEECCCCCCCEEEEEEEHHHCCCCEECCCCCCCCCCCCCCCCCCCCEEEECCCC
NGRLSDLRDAAAILKNRKVSDSVRMLVIPASREVYRRALDEGLIEIFVDAGALVCNPCCG
CCCHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHEEEECCCCEEECCCCC
PCLGGHVGLVGPGEVSLSTSNRNFRGRQGSPEAEVYLSSAAVAAASAVKGSITHPGSLK
CCCCCCEEEECCCCEEEEECCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SMTVSEKILARASGKDRVEAGEIVMADIDVAMTHDLTGPLSVESFRAIGEDRVWDPEKI
CCCHHHHHHHHCCCCCCCCCCCEEEEECCEEEECCCCCCCCHHHHHHCCCCCCCCCCEE
VVIFDHQVPADSIEAAQNHMIMRDFVEEQGIRNFYDVREGVCHQVLPEKGHVVPGEVVVG
EEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
TDSHTCTHGALGAFATGIGSTDMAMVFATGKLWFRVPETLRFDVRGKLREHVYAKDVILN
CCCCCCCCCCHHHHHHCCCCCCEEEEEEECEEEEECCCCEEECCHHHHHHHHHHHHHHHH
IIGRVGADGATYMACEFAGETVAEMSVSDRMVLSNMAIEMGGKTGIVEPDEKTLNYVRRR
HHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHC
SGKPWRVFKTDPDAPSLSVMEVDVSDLEPQVACPHNVDNVKPVTEVEGTEIDQVFLGSCT
CCCCEEEEECCCCCCCEEEEEEEHHHCCCCEECCCCCCCCCCCCCCCCCCCCEEEECCCC
NGRLSDLRDAAAILKNRKVSDSVRMLVIPASREVYRRALDEGLIEIFVDAGALVCNPCCG
CCCHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHEEEECCCCEEECCCCC
PCLGGHVGLVGPGEVSLSTSNRNFRGRQGSPEAEVYLSSAAVAAASAVKGSITHPGSLK
CCCCCCEEEECCCCEEEEECCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463