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Identifier: 15678612

GI number: 15678612

Start: 522010

End: 523374

Strand: Direct

Name: Not Available

Synonym: MTH584

Alternate gene names: 15678612

Gene position: 522010-523374 (Clockwise)

Preceding gene: 15678611

Following gene: 15678615

Centisome position: 29.81

GC content: 53.48

Gene sequence:

>1365_bases
ATGACCACAGACTACAGCAACATACTCAAAACCATAAAACCCGACGGTGATGAATACCGGAGGGTCATGGAACTATCAGA
CAGTCTCGTTGAGTGCCTGAATGGACTCGCAGAGGAACAGGGAATAGACGCCGAGGCCGTCCTCGTGGGTTCAGTTGCCA
AGGGGACCTGGCTTTCAGGTGCTGCAGACATTGACATATTCATACACTTTCCCCTCACGACCCCAGAGGATGAACTCAAG
GAGAGTGGCCTCAGACTCGGCTATGGATGCATAGAGAGGTTCGGTGGTGAAGCCGAGGAAAGATACGCATCCCACCCCTA
TGTAACCGGTTACATTGACGGCTATGAGGTGGACTTCGTCCCCTGTTACCGGATAGATGATGCATCCATGTTAAGGTCGG
CCGTTGATCGGACCATACTCCACACAAGATATATACAGGAGAACCTCAGGGATGAGCAGAGAGATGATGTGCTCCTCCTT
AAGCAGTTCATGAAGTCAACAGGGACCTACGGTTCAGAGTTCCGTGTTGGTGGCTTCGCAGGGTACCTTGCCGAGCTGCT
GGTGCTCCGCTACGGCGACTTTGAGGGAGTCCTCGAGGGTGCCCTTCAGTGGAGGCCCGGATACATAATAGACCTTGAGG
GCCACGGGACAGGTAAGGGATTCGAGGATCCCCTGGTGGTGGTTGACCCTGTTGATAGAAACAGAAACGTTGCAGCGGCC
CTGACACTCCAGAGGATGGCTGAATTTGTAACCGCATCCCTGAACTTCCTGGGGAACCCGAGCCCAGAGTACTTTCAACC
CCCCAGCTACTCCACAGATGCCGCTGAAATAACCGGGACCCTCAGCTGCAGGGGTTCCAGGGTCATCGTCATCTCAACGG
GAGTCCCGGATGTACCCTCAGATGCCCTCTACCCCCAGCTGAGGAAGACCCTTGATTCTGTGGTTAAGAACCTTGAGGCG
GAGGGTTTCTCTGTCCTGGGAGCTGACTACTGGAGTGATGAGAGCAAGTCAACATTTATGGTCCTTGAGATGGATGTATG
GGAGCTCCCTTCATACCTGAAGAGGTATGGTCCCCCTGTATGGTCCCGACGCCACAGGGAGAGATTCCTCAGGAAGCATG
AGAGGGTCTGGGTTGAGGGATCCAGGCTCACCATAGAATCCCCCAGGAGGCACAGATCCGCAGTTTCATATCTGAGGGAC
CTGCTTTCAAAGCCGGATAGGCTGAGGATGGGTAAACATATAGGGGTGGAGATCAGGAGGGGCTTCAGTGTTGATCTCCT
TGATGATGTAATCGGAGAAGCTGAAAAGGAATTTCTGGAGTTCATGGATGCATTCCTGAACCCCTGGAAGGCCCTTGAAA
GATGA

Upstream 100 bases:

>100_bases
TTCATGGCCCCCTATATGGATAGCTGGTGCACTCTCACTCCGGTTCCTATCCTGGATTAGTTTATCTGCAGTTTACTTCA
ATTCTTCATGGTGATGATTT

Downstream 100 bases:

>100_bases
TCCCATAACCCCCTACTATCTACTAACCCGGAAAGGCTATTCCCTCTCATCCACAAACCTGGGGACAGCGCTCTTAAGGG
GGTCAAAGGTCTCAGGATTT

Product: tRNA CCA-pyrophosphorylase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MTTDYSNILKTIKPDGDEYRRVMELSDSLVECLNGLAEEQGIDAEAVLVGSVAKGTWLSGAADIDIFIHFPLTTPEDELK
ESGLRLGYGCIERFGGEAEERYASHPYVTGYIDGYEVDFVPCYRIDDASMLRSAVDRTILHTRYIQENLRDEQRDDVLLL
KQFMKSTGTYGSEFRVGGFAGYLAELLVLRYGDFEGVLEGALQWRPGYIIDLEGHGTGKGFEDPLVVVDPVDRNRNVAAA
LTLQRMAEFVTASLNFLGNPSPEYFQPPSYSTDAAEITGTLSCRGSRVIVISTGVPDVPSDALYPQLRKTLDSVVKNLEA
EGFSVLGADYWSDESKSTFMVLEMDVWELPSYLKRYGPPVWSRRHRERFLRKHERVWVEGSRLTIESPRRHRSAVSYLRD
LLSKPDRLRMGKHIGVEIRRGFSVDLLDDVIGEAEKEFLEFMDAFLNPWKALER

Sequences:

>Translated_454_residues
MTTDYSNILKTIKPDGDEYRRVMELSDSLVECLNGLAEEQGIDAEAVLVGSVAKGTWLSGAADIDIFIHFPLTTPEDELK
ESGLRLGYGCIERFGGEAEERYASHPYVTGYIDGYEVDFVPCYRIDDASMLRSAVDRTILHTRYIQENLRDEQRDDVLLL
KQFMKSTGTYGSEFRVGGFAGYLAELLVLRYGDFEGVLEGALQWRPGYIIDLEGHGTGKGFEDPLVVVDPVDRNRNVAAA
LTLQRMAEFVTASLNFLGNPSPEYFQPPSYSTDAAEITGTLSCRGSRVIVISTGVPDVPSDALYPQLRKTLDSVVKNLEA
EGFSVLGADYWSDESKSTFMVLEMDVWELPSYLKRYGPPVWSRRHRERFLRKHERVWVEGSRLTIESPRRHRSAVSYLRD
LLSKPDRLRMGKHIGVEIRRGFSVDLLDDVIGEAEKEFLEFMDAFLNPWKALER
>Mature_453_residues
TTDYSNILKTIKPDGDEYRRVMELSDSLVECLNGLAEEQGIDAEAVLVGSVAKGTWLSGAADIDIFIHFPLTTPEDELKE
SGLRLGYGCIERFGGEAEERYASHPYVTGYIDGYEVDFVPCYRIDDASMLRSAVDRTILHTRYIQENLRDEQRDDVLLLK
QFMKSTGTYGSEFRVGGFAGYLAELLVLRYGDFEGVLEGALQWRPGYIIDLEGHGTGKGFEDPLVVVDPVDRNRNVAAAL
TLQRMAEFVTASLNFLGNPSPEYFQPPSYSTDAAEITGTLSCRGSRVIVISTGVPDVPSDALYPQLRKTLDSVVKNLEAE
GFSVLGADYWSDESKSTFMVLEMDVWELPSYLKRYGPPVWSRRHRERFLRKHERVWVEGSRLTIESPRRHRSAVSYLRDL
LSKPDRLRMGKHIGVEIRRGFSVDLLDDVIGEAEKEFLEFMDAFLNPWKALER

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG1746

COG function: function code J; tRNA nucleotidyltransferase (CCA-adding enzyme)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Archaeal CCA-adding enzyme subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_METTH (O26684)

Other databases:

- EMBL:   AE000666
- PIR:   D69177
- RefSeq:   NP_275727.1
- ProteinModelPortal:   O26684
- SMR:   O26684
- STRING:   O26684
- GeneID:   1470545
- GenomeReviews:   AE000666_GR
- KEGG:   mth:MTH584
- NMPDR:   fig|187420.1.peg.582
- eggNOG:   arNOG05007
- HOGENOM:   HBG645243
- OMA:   CEKIISA
- ProtClustDB:   PRK13300
- BioCyc:   MTHE187420:MTH584-MONOMER
- BRENDA:   2.7.7.21
- BRENDA:   2.7.7.25
- HAMAP:   MF_01264
- InterPro:   IPR008229
- InterPro:   IPR002934
- InterPro:   IPR011068
- InterPro:   IPR015329
- PIRSF:   PIRSF005335
- TIGRFAMs:   TIGR03671

Pfam domain/function: PF01909 NTP_transf_2; PF09249 tRNA_NucTransf2; SSF55003 PAP_C

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 51346; Mature: 51214

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: BINDING 51-51 BINDING 54-54 BINDING 141-141 BINDING 161-161 BINDING 170-170

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTDYSNILKTIKPDGDEYRRVMELSDSLVECLNGLAEEQGIDAEAVLVGSVAKGTWLSG
CCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEECCCCCCCCCC
AADIDIFIHFPLTTPEDELKESGLRLGYGCIERFGGEAEERYASHPYVTGYIDGYEVDFV
CCCEEEEEEECCCCCHHHHHHCCCEECHHHHHHHCCCHHHHHCCCCEEEEEECCEEEEEE
PCYRIDDASMLRSAVDRTILHTRYIQENLRDEQRDDVLLLKQFMKSTGTYGSEFRVGGFA
EEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEECCHHH
GYLAELLVLRYGDFEGVLEGALQWRPGYIIDLEGHGTGKGFEDPLVVVDPVDRNRNVAAA
HHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEECCCCCCCCHHHH
LTLQRMAEFVTASLNFLGNPSPEYFQPPSYSTDAAEITGTLSCRGSRVIVISTGVPDVPS
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHEEEEEECCCEEEEEECCCCCCCC
DALYPQLRKTLDSVVKNLEAEGFSVLGADYWSDESKSTFMVLEMDVWELPSYLKRYGPPV
HHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCH
WSRRHRERFLRKHERVWVEGSRLTIESPRRHRSAVSYLRDLLSKPDRLRMGKHIGVEIRR
HHHHHHHHHHHHHHCEEEECCEEEECCCHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEEC
GFSVDLLDDVIGEAEKEFLEFMDAFLNPWKALER
CCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCC
>Mature Secondary Structure 
TTDYSNILKTIKPDGDEYRRVMELSDSLVECLNGLAEEQGIDAEAVLVGSVAKGTWLSG
CCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEECCCCCCCCCC
AADIDIFIHFPLTTPEDELKESGLRLGYGCIERFGGEAEERYASHPYVTGYIDGYEVDFV
CCCEEEEEEECCCCCHHHHHHCCCEECHHHHHHHCCCHHHHHCCCCEEEEEECCEEEEEE
PCYRIDDASMLRSAVDRTILHTRYIQENLRDEQRDDVLLLKQFMKSTGTYGSEFRVGGFA
EEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEECCHHH
GYLAELLVLRYGDFEGVLEGALQWRPGYIIDLEGHGTGKGFEDPLVVVDPVDRNRNVAAA
HHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEECCCCCCCCHHHH
LTLQRMAEFVTASLNFLGNPSPEYFQPPSYSTDAAEITGTLSCRGSRVIVISTGVPDVPS
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHEEEEEECCCEEEEEECCCCCCCC
DALYPQLRKTLDSVVKNLEAEGFSVLGADYWSDESKSTFMVLEMDVWELPSYLKRYGPPV
HHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCH
WSRRHRERFLRKHERVWVEGSRLTIESPRRHRSAVSYLRDLLSKPDRLRMGKHIGVEIRR
HHHHHHHHHHHHHHCEEEECCEEEECCCHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEEC
GFSVDLLDDVIGEAEKEFLEFMDAFLNPWKALER
CCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463