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The map label for this gene is metG

Identifier: 15678615

GI number: 15678615

Start: 525947

End: 527902

Strand: Direct

Name: metG

Synonym: MTH587

Alternate gene names: 15678615

Gene position: 525947-527902 (Clockwise)

Preceding gene: 15678612

Following gene: 15678616

Centisome position: 30.03

GC content: 53.99

Gene sequence:

>1956_bases
TTGAGTAAAGTATTTATCACCTGCGCACTTCCATATGCCAACGGCCCCACGCATCTCGGTCATCTGAGGTCAACCTACAT
ACCTGCAGACATATATGCGAGGTACCGGAGACTCAGGGGCGATGATGTCCTCTTCGTCTGTGCCACCGATGAACACGGAA
CTCCCATAGCCGTGAAGGCAGAGGCCGAGGGCAGGACCCCCCTGGAGGTCGCCACCCGCTACCATGAAATGATAAGGGGG
GACCTTGAGAGGTGTGACATATCCTTTGACAGTTTCACCAGGACCACAGATGAGCTCCACCACGAGATAGCCCAGAAATT
CTTCCTTAAACTCTACGAGAAGGGCTTCATATATGAGAAGGACATAAAACAGCTCTACTGTGGGGACTGCCAGCGATTCC
TCCCTGACAGGTACGTTGAGGGCACCTGCCCCTACTGTGGAAGTGAGGGTGCGCGTGGAGACCACTGCGAGGGCTGTGGA
AGGCACCTTGAGCCGCTCCAGCTTGAGGATCCGCGCTGTATGGTATGCGGGTCAGAACCAGAGGTCAGGGACTCCCGGCA
CTACTTCTTCCGCCTGAACCAGTTCCAGGATGAAATCAGGGAGTGGATCGAGGGCTCAGAGATGCCATCAAATGTGAGAA
ATTACGCCATCCAGTGGCTCCGCGAGGGCCTGAAGGACTGGATACTCACAAGGGACATGGAATGGGGTGTCCAGGTACCA
CTGGAGGGGAACGAGGGTAAGATCATATACGTGTGGGGTGAGGCCTTCCTTGGCTACATCTCATCTGCAGCCGCCTGGAG
CAAAAAAACCGGGAGGGACTGGAGAGAATACTGGGACTCCGGCGCCATCCACTTCATCGGAAAGGACATAATATACCACC
ACGCCATATTCTGGCCAGCCCTCCTGATGGCGTATGGCTGCAGGACCCCCGCGAACATAATAGCCGGGGAGTACCTGTCA
CTTGAGGGCCAGAAGATGTCCACCAGTAAAAACTGGGTGGTCTGGACATCAGACTTCCTTGAAAGGTTTGACAGGGACCT
TCTCAGGTACTACCTCACCGTCAACGCACCCCTCACCAGGGACACTGACTTCTCATGGGACGACTTCCAGAGGAGGGTTA
ACGATGAACTGGCCGATGTCCTCGGGAACTTCCTGCACAGGACCTTCTCATTCACAGGTAGGTTCTTTGACGGGAGGGTC
CCGGCTGCAGGTGAACTTACAGCTGAGGACCTGGAATTCCTTGAATCAATCAGGGCCGCCCACGACACCGTCGCTGAACT
CCTGGACAAATTCCAGTTCAGGGATGCCCTGATGCACATAATCAAACTTGCCAAGAGGGGTAACAAGTACTTCAATGACC
AGGAACCCTGGAAGGCCGTTAAGGAGTCACCCGCCAGGGCCTCAACCTGCCTCCACCTCTGCAATCTCCTGGCAGCCAAC
CTCGGGAAACTCTTGAGGCCATTCATGCCATCAGCAGCAGGCAGGATACTCTCCATCATGAACCTGGAGGATGAAGCCTG
GGGATTCCATGAACTCAGGGAGGGACAGGTGATAGAGAGGGCGAAGCCCCTCTTCTCCAAGATAACTGACGAGGCAATTG
AGGAGGAGAAAGCAAAACTCATTGAGGAGGATGATGAAGTGGAAACCGTGACAATCGACGACTTTACAACTATGGACATA
AGGGTTGGCGTGATAAGATCAGCCGAGCGAATAGAGGGATCAGACAAGCTCCTGAAGCTCATAATAGATGTGGGTGAGAG
GGAGATGCAGGTTGTTGCAGGACTTGCAGAGAAATACAGCCCCGAAGATCTTGTTGAGAGGAAGATTACAGTGCTTGTGA
ACCTCAAACCCGCGAAACTCTTCGGTGTGAAGTCAGAGGGCATGGTACTTGCAACAGGAGAATCCCTCAACATACTCGAC
CCCGGGGACGCCGAAGTCGGTGAAAGGATAAGGTGA

Upstream 100 bases:

>100_bases
AAATTTATATAGAAGTAAACCGTAAATTAAACTGGATTTAATTGCCTTAAATCGACCCGTCTTATTCTGATCTGGTTCAT
GATTCAAGGAGGAGTTTGAG

Downstream 100 bases:

>100_bases
GTGGAAGGCCCTGTACCCTGTGTCTGGAGAATGATAAATGAATGAATCAGGTCTCATTGCAAGGTCAGAGAGGTTCCTTG
AGAGCATAAAGGACAGGAAG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 651; Mature: 650

Protein sequence:

>651_residues
MSKVFITCALPYANGPTHLGHLRSTYIPADIYARYRRLRGDDVLFVCATDEHGTPIAVKAEAEGRTPLEVATRYHEMIRG
DLERCDISFDSFTRTTDELHHEIAQKFFLKLYEKGFIYEKDIKQLYCGDCQRFLPDRYVEGTCPYCGSEGARGDHCEGCG
RHLEPLQLEDPRCMVCGSEPEVRDSRHYFFRLNQFQDEIREWIEGSEMPSNVRNYAIQWLREGLKDWILTRDMEWGVQVP
LEGNEGKIIYVWGEAFLGYISSAAAWSKKTGRDWREYWDSGAIHFIGKDIIYHHAIFWPALLMAYGCRTPANIIAGEYLS
LEGQKMSTSKNWVVWTSDFLERFDRDLLRYYLTVNAPLTRDTDFSWDDFQRRVNDELADVLGNFLHRTFSFTGRFFDGRV
PAAGELTAEDLEFLESIRAAHDTVAELLDKFQFRDALMHIIKLAKRGNKYFNDQEPWKAVKESPARASTCLHLCNLLAAN
LGKLLRPFMPSAAGRILSIMNLEDEAWGFHELREGQVIERAKPLFSKITDEAIEEEKAKLIEEDDEVETVTIDDFTTMDI
RVGVIRSAERIEGSDKLLKLIIDVGEREMQVVAGLAEKYSPEDLVERKITVLVNLKPAKLFGVKSEGMVLATGESLNILD
PGDAEVGERIR

Sequences:

>Translated_651_residues
MSKVFITCALPYANGPTHLGHLRSTYIPADIYARYRRLRGDDVLFVCATDEHGTPIAVKAEAEGRTPLEVATRYHEMIRG
DLERCDISFDSFTRTTDELHHEIAQKFFLKLYEKGFIYEKDIKQLYCGDCQRFLPDRYVEGTCPYCGSEGARGDHCEGCG
RHLEPLQLEDPRCMVCGSEPEVRDSRHYFFRLNQFQDEIREWIEGSEMPSNVRNYAIQWLREGLKDWILTRDMEWGVQVP
LEGNEGKIIYVWGEAFLGYISSAAAWSKKTGRDWREYWDSGAIHFIGKDIIYHHAIFWPALLMAYGCRTPANIIAGEYLS
LEGQKMSTSKNWVVWTSDFLERFDRDLLRYYLTVNAPLTRDTDFSWDDFQRRVNDELADVLGNFLHRTFSFTGRFFDGRV
PAAGELTAEDLEFLESIRAAHDTVAELLDKFQFRDALMHIIKLAKRGNKYFNDQEPWKAVKESPARASTCLHLCNLLAAN
LGKLLRPFMPSAAGRILSIMNLEDEAWGFHELREGQVIERAKPLFSKITDEAIEEEKAKLIEEDDEVETVTIDDFTTMDI
RVGVIRSAERIEGSDKLLKLIIDVGEREMQVVAGLAEKYSPEDLVERKITVLVNLKPAKLFGVKSEGMVLATGESLNILD
PGDAEVGERIR
>Mature_650_residues
SKVFITCALPYANGPTHLGHLRSTYIPADIYARYRRLRGDDVLFVCATDEHGTPIAVKAEAEGRTPLEVATRYHEMIRGD
LERCDISFDSFTRTTDELHHEIAQKFFLKLYEKGFIYEKDIKQLYCGDCQRFLPDRYVEGTCPYCGSEGARGDHCEGCGR
HLEPLQLEDPRCMVCGSEPEVRDSRHYFFRLNQFQDEIREWIEGSEMPSNVRNYAIQWLREGLKDWILTRDMEWGVQVPL
EGNEGKIIYVWGEAFLGYISSAAAWSKKTGRDWREYWDSGAIHFIGKDIIYHHAIFWPALLMAYGCRTPANIIAGEYLSL
EGQKMSTSKNWVVWTSDFLERFDRDLLRYYLTVNAPLTRDTDFSWDDFQRRVNDELADVLGNFLHRTFSFTGRFFDGRVP
AAGELTAEDLEFLESIRAAHDTVAELLDKFQFRDALMHIIKLAKRGNKYFNDQEPWKAVKESPARASTCLHLCNLLAANL
GKLLRPFMPSAAGRILSIMNLEDEAWGFHELREGQVIERAKPLFSKITDEAIEEEKAKLIEEDDEVETVTIDDFTTMDIR
VGVIRSAERIEGSDKLLKLIIDVGEREMQVVAGLAEKYSPEDLVERKITVLVNLKPAKLFGVKSEGMVLATGESLNILDP
GDAEVGERIR

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI14043022, Length=574, Percent_Identity=37.4564459930314, Blast_Score=367, Evalue=1e-101,
Organism=Homo sapiens, GI24308436, Length=536, Percent_Identity=29.4776119402985, Blast_Score=213, Evalue=5e-55,
Organism=Escherichia coli, GI1788432, Length=680, Percent_Identity=35.2941176470588, Blast_Score=408, Evalue=1e-115,
Organism=Escherichia coli, GI1786861, Length=237, Percent_Identity=24.4725738396624, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1789455, Length=84, Percent_Identity=41.6666666666667, Blast_Score=69, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17540794, Length=615, Percent_Identity=33.4959349593496, Blast_Score=350, Evalue=1e-96,
Organism=Caenorhabditis elegans, GI212645096, Length=530, Percent_Identity=26.0377358490566, Blast_Score=171, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI71992781, Length=421, Percent_Identity=24.2280285035629, Blast_Score=115, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6321704, Length=557, Percent_Identity=32.3159784560144, Blast_Score=315, Evalue=2e-86,
Organism=Saccharomyces cerevisiae, GI6321610, Length=555, Percent_Identity=24.8648648648649, Blast_Score=136, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24655311, Length=559, Percent_Identity=36.4937388193202, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI21358483, Length=540, Percent_Identity=28.8888888888889, Blast_Score=196, Evalue=6e-50,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_METTH (O26687)

Other databases:

- EMBL:   AE000666
- PIR:   G69177
- RefSeq:   NP_275730.1
- ProteinModelPortal:   O26687
- SMR:   O26687
- STRING:   O26687
- GeneID:   1470548
- GenomeReviews:   AE000666_GR
- KEGG:   mth:MTH587
- NMPDR:   fig|187420.1.peg.585
- eggNOG:   arNOG04371
- HOGENOM:   HBG721667
- OMA:   CGNQLDP
- PhylomeDB:   O26687
- ProtClustDB:   PRK00133
- BioCyc:   MTHE187420:MTH587-MONOMER
- BRENDA:   6.1.1.10
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 74633; Mature: 74501

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD

Important sites: BINDING 328-328

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVFITCALPYANGPTHLGHLRSTYIPADIYARYRRLRGDDVLFVCATDEHGTPIAVKA
CCEEEEEEEECCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEE
EAEGRTPLEVATRYHEMIRGDLERCDISFDSFTRTTDELHHEIAQKFFLKLYEKGFIYEK
CCCCCCHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
DIKQLYCGDCQRFLPDRYVEGTCPYCGSEGARGDHCEGCGRHLEPLQLEDPRCMVCGSEP
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCEEEECCCC
EVRDSRHYFFRLNQFQDEIREWIEGSEMPSNVRNYAIQWLREGLKDWILTRDMEWGVQVP
CCCCCCHHEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
LEGNEGKIIYVWGEAFLGYISSAAAWSKKTGRDWREYWDSGAIHFIGKDIIYHHAIFWPA
EECCCCEEEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEHHHHHHHHHHHHHH
LLMAYGCRTPANIIAGEYLSLEGQKMSTSKNWVVWTSDFLERFDRDLLRYYLTVNAPLTR
HHHHHCCCCCHHHHCCCCEECCCCEECCCCCEEEECHHHHHHHHHHHHHHHHEECCCCCC
DTDFSWDDFQRRVNDELADVLGNFLHRTFSFTGRFFDGRVPAAGELTAEDLEFLESIRAA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
HDTVAELLDKFQFRDALMHIIKLAKRGNKYFNDQEPWKAVKESPARASTCLHLCNLLAAN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHH
LGKLLRPFMPSAAGRILSIMNLEDEAWGFHELREGQVIERAKPLFSKITDEAIEEEKAKL
HHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
IEEDDEVETVTIDDFTTMDIRVGVIRSAERIEGSDKLLKLIIDVGEREMQVVAGLAEKYS
CCCCCCEEEEEECCEEEEEEEEEHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHCC
PEDLVERKITVLVNLKPAKLFGVKSEGMVLATGESLNILDPGDAEVGERIR
HHHHHCCEEEEEEEECCHHEEEECCCCEEEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SKVFITCALPYANGPTHLGHLRSTYIPADIYARYRRLRGDDVLFVCATDEHGTPIAVKA
CEEEEEEEECCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEE
EAEGRTPLEVATRYHEMIRGDLERCDISFDSFTRTTDELHHEIAQKFFLKLYEKGFIYEK
CCCCCCHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
DIKQLYCGDCQRFLPDRYVEGTCPYCGSEGARGDHCEGCGRHLEPLQLEDPRCMVCGSEP
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCEEEECCCC
EVRDSRHYFFRLNQFQDEIREWIEGSEMPSNVRNYAIQWLREGLKDWILTRDMEWGVQVP
CCCCCCHHEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
LEGNEGKIIYVWGEAFLGYISSAAAWSKKTGRDWREYWDSGAIHFIGKDIIYHHAIFWPA
EECCCCEEEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEHHHHHHHHHHHHHH
LLMAYGCRTPANIIAGEYLSLEGQKMSTSKNWVVWTSDFLERFDRDLLRYYLTVNAPLTR
HHHHHCCCCCHHHHCCCCEECCCCEECCCCCEEEECHHHHHHHHHHHHHHHHEECCCCCC
DTDFSWDDFQRRVNDELADVLGNFLHRTFSFTGRFFDGRVPAAGELTAEDLEFLESIRAA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
HDTVAELLDKFQFRDALMHIIKLAKRGNKYFNDQEPWKAVKESPARASTCLHLCNLLAAN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHH
LGKLLRPFMPSAAGRILSIMNLEDEAWGFHELREGQVIERAKPLFSKITDEAIEEEKAKL
HHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
IEEDDEVETVTIDDFTTMDIRVGVIRSAERIEGSDKLLKLIIDVGEREMQVVAGLAEKYS
CCCCCCEEEEEECCEEEEEEEEEHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHCC
PEDLVERKITVLVNLKPAKLFGVKSEGMVLATGESLNILDPGDAEVGERIR
HHHHHCCEEEEEEEECCHHEEEECCCCEEEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463