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The map label for this gene is hisS [H]

Identifier: 13507784

GI number: 13507784

Start: 53077

End: 54321

Strand: Direct

Name: hisS [H]

Synonym: MPN045

Alternate gene names: 13507784

Gene position: 53077-54321 (Clockwise)

Preceding gene: 13507782

Following gene: 13507785

Centisome position: 6.5

GC content: 45.22

Gene sequence:

>1245_bases
ATGAGTGTGTTGCAAAAACCGCGCGGGGTTAAAGACTGGTATGGTGAGGAGTTAATCTACTTCAATTGAACGGTGCACCA
GATCACTAATTTGGCCTGAAAATGAGGCTTTAGTGAGGTCAAAACACCCCTGTTGGAATACGCTGAAGCCTTTAAGCGCA
CCAATGCCAACGCCGATATTGTCAAGAAGGAGTTGTATGAATTCCATGACAAATCGAACCGGCTGTTAGCCCTGCGTCCC
GAGGCTACTGCTGGGATAGTGCGCTTGGTCTGTGAGAACAAACTACTCCAACCACAAAACTACCCGTTAAGGTTGTTTAC
CATTGGCACCATGTACCGGTATGAACGCCCCCAAAGTAACCGTTACCGCGAGCACTACCAGTTTAGTTGTGAAGTGATAG
GTGACACTAATCCAACCGTGTTACTCGACACTTTACTGTTGGGTCATGCCATTATCCAGCAATTGGGAATTGAAGGAGTG
ATCCTCAAACTTAACAACTTAGGTAACAGTGCTACCATTCAACAATGAAACCAAGCGCTGCAAGCTTACTTAACCCAGTT
TAAGGCGCAGTTAACCGAGTTATCACAAAGCCGTTTATCGACCAATCCTCTAAGGATCTTGGACGACAAGGTTGATGGTC
AACTACCCTTTATCAGTGATGCCCCCCAAATTGAGCAGTTCTTGGATGCTGAGCAGCAGGCACTGAATACTTGACTGCAG
CAACAGTTAACCCAACAACAGGTGCCGTTTGAGTGGAATCCAACCCTAGTCCGGGGCTTGGACTACTATACTGGGGTAGT
GTTTGAATTTGTTAAGGATGACACCACGGTACTAGCTGGTGGGGTGTATGATAACCTAGTGGAAGAGTTAGGTGGTACAC
CTACTAAAGCGCTGGGTTTTGCTTGTGGCATTGAACGCTCAATTAATTGTTTAAGTGCGGTTAAAAAGCAAGCAATTTTG
GCCAATCAACCCCCACGATTACTGGTTATAGGTCTCACCGAAGCAGCGTTGGAAAAGCTGTTACAACTTTCATTGGGTTG
AAGGGCGTATCATCCTGTGACTATTTATCCTAAAGTTATTAGAATTATCAATGGCATCAGAGCCGCGCAACGGTTAGGTT
ACCGCTTTTTAGGGGTCATTGGTGGTAATAATTTAGAACAGCAAACCATTACCGTTAAAGACCTGGCTACTGAACAGCAA
ACAACATACACTTGAGATGAATTTAGACAACGCCAAGTGCTTTAA

Upstream 100 bases:

>100_bases
CCGGAAAACATCGGCCCACAAATCACTTCGATTCAACCACGTGGGGATTGGTGGAAAACTTGGGAAAAGCTCATAGTCGC
CCAATTATAATTAAACAAAG

Downstream 100 bases:

>100_bases
GCAACGGGTTTTTATTGGCAATTTAACTTCCGAGCACCTCAATAAAACCGTTACTATTGCCGGATGGGTCAAACGGATTA
AGAAGTTAGGTGAACTTAAC

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MSVLQKPRGVKDWYGEELIYFNWTVHQITNLAWKWGFSEVKTPLLEYAEAFKRTNANADIVKKELYEFHDKSNRLLALRP
EATAGIVRLVCENKLLQPQNYPLRLFTIGTMYRYERPQSNRYREHYQFSCEVIGDTNPTVLLDTLLLGHAIIQQLGIEGV
ILKLNNLGNSATIQQWNQALQAYLTQFKAQLTELSQSRLSTNPLRILDDKVDGQLPFISDAPQIEQFLDAEQQALNTWLQ
QQLTQQQVPFEWNPTLVRGLDYYTGVVFEFVKDDTTVLAGGVYDNLVEELGGTPTKALGFACGIERSINCLSAVKKQAIL
ANQPPRLLVIGLTEAALEKLLQLSLGWRAYHPVTIYPKVIRIINGIRAAQRLGYRFLGVIGGNNLEQQTITVKDLATEQQ
TTYTWDEFRQRQVL

Sequences:

>Translated_414_residues
MSVLQKPRGVKDWYGEELIYFN*TVHQITNLA*K*GFSEVKTPLLEYAEAFKRTNANADIVKKELYEFHDKSNRLLALRP
EATAGIVRLVCENKLLQPQNYPLRLFTIGTMYRYERPQSNRYREHYQFSCEVIGDTNPTVLLDTLLLGHAIIQQLGIEGV
ILKLNNLGNSATIQQ*NQALQAYLTQFKAQLTELSQSRLSTNPLRILDDKVDGQLPFISDAPQIEQFLDAEQQALNT*LQ
QQLTQQQVPFEWNPTLVRGLDYYTGVVFEFVKDDTTVLAGGVYDNLVEELGGTPTKALGFACGIERSINCLSAVKKQAIL
ANQPPRLLVIGLTEAALEKLLQLSLG*RAYHPVTIYPKVIRIINGIRAAQRLGYRFLGVIGGNNLEQQTITVKDLATEQQ
TTYT*DEFRQRQVL
>Mature_413_residues
SVLQKPRGVKDWYGEELIYFN*TVHQITNLA*K*GFSEVKTPLLEYAEAFKRTNANADIVKKELYEFHDKSNRLLALRPE
ATAGIVRLVCENKLLQPQNYPLRLFTIGTMYRYERPQSNRYREHYQFSCEVIGDTNPTVLLDTLLLGHAIIQQLGIEGVI
LKLNNLGNSATIQQ*NQALQAYLTQFKAQLTELSQSRLSTNPLRILDDKVDGQLPFISDAPQIEQFLDAEQQALNT*LQQ
QLTQQQVPFEWNPTLVRGLDYYTGVVFEFVKDDTTVLAGGVYDNLVEELGGTPTKALGFACGIERSINCLSAVKKQAILA
NQPPRLLVIGLTEAALEKLLQLSLG*RAYHPVTIYPKVIRIINGIRAAQRLGYRFLGVIGGNNLEQQTITVKDLATEQQT
TYT*DEFRQRQVL

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788861, Length=414, Percent_Identity=35.024154589372, Blast_Score=207, Evalue=8e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 45833; Mature: 45702

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVLQKPRGVKDWYGEELIYFNTVHQITNLAKGFSEVKTPLLEYAEAFKRTNANADIVKK
CCCCCCCCCCHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ELYEFHDKSNRLLALRPEATAGIVRLVCENKLLQPQNYPLRLFTIGTMYRYERPQSNRYR
HHHHHHCCCCCEEEECCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEHHHHCCCCCCCCCH
EHYQFSCEVIGDTNPTVLLDTLLLGHAIIQQLGIEGVILKLNNLGNSATIQQNQALQAYL
HHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEHHHHHHHHHH
TQFKAQLTELSQSRLSTNPLRILDDKVDGQLPFISDAPQIEQFLDAEQQALNTLQQQLTQ
HHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QQVPFEWNPTLVRGLDYYTGVVFEFVKDDTTVLAGGVYDNLVEELGGTPTKALGFACGIE
HCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHCCCCHHHHHHHHCHH
RSINCLSAVKKQAILANQPPRLLVIGLTEAALEKLLQLSLGRAYHPVTIYPKVIRIINGI
HHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHH
RAAQRLGYRFLGVIGGNNLEQQTITVKDLATEQQTTYTDEFRQRQVL
HHHHHCCCCEEEEECCCCCCHHEEEHHHHHCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SVLQKPRGVKDWYGEELIYFNTVHQITNLAKGFSEVKTPLLEYAEAFKRTNANADIVKK
CCCCCCCCCHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ELYEFHDKSNRLLALRPEATAGIVRLVCENKLLQPQNYPLRLFTIGTMYRYERPQSNRYR
HHHHHHCCCCCEEEECCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEHHHHCCCCCCCCCH
EHYQFSCEVIGDTNPTVLLDTLLLGHAIIQQLGIEGVILKLNNLGNSATIQQNQALQAYL
HHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEHHHHHHHHHH
TQFKAQLTELSQSRLSTNPLRILDDKVDGQLPFISDAPQIEQFLDAEQQALNTLQQQLTQ
HHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QQVPFEWNPTLVRGLDYYTGVVFEFVKDDTTVLAGGVYDNLVEELGGTPTKALGFACGIE
HCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHCCCCHHHHHHHHCHH
RSINCLSAVKKQAILANQPPRLLVIGLTEAALEKLLQLSLGRAYHPVTIYPKVIRIINGI
HHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHH
RAAQRLGYRFLGVIGGNNLEQQTITVKDLATEQQTTYTDEFRQRQVL
HHHHHCCCCEEEEECCCCCCHHEEEHHHHHCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633; 11271496 [H]