Click here to switch to the map view.

The map label for this gene is aspS [H]

Identifier: 13507785

GI number: 13507785

Start: 54293

End: 55966

Strand: Direct

Name: aspS [H]

Synonym: MPN046

Alternate gene names: 13507785

Gene position: 54293-55966 (Clockwise)

Preceding gene: 13507784

Following gene: 13507786

Centisome position: 6.65

GC content: 42.95

Gene sequence:

>1674_bases
ATGAATTTAGACAACGCCAAGTGCTTTAAGCAACGGGTTTTTATTGGCAATTTAACTTCCGAGCACCTCAATAAAACCGT
TACTATTGCCGGATGGGTCAAACGGATTAAGAAGTTAGGTGAACTTAACTTTGTTATTGTCGGGGACAAGACTAATACTA
TTCAGGTTACCTGTAAAAATAAGGAACAGGTCAAGTACTTAACCAGGGAAGACTTGGTGATTGTCAAGGGCAAACTGAAA
CGCTTAGACAGTGTTCGGTTTGAAATTACCAATCCTACTATTACCTTATTTGCCAAGTCCAAAACACCCCCTCTTATCAT
TGAGGACAAAACTGATGCTTTAGAAGAAGTGCGTTTGCGTTACCGCTACTTAGACTTACGCCGTCCGGTGATGCAAAAGC
GCTTGGCGTTACGCCATAAGGTAACTTTAGCCGTCCGGAACTGACTAGATCAAATGGGGTTTATTGAAGTAGAAACACCT
ACGCTCACGAAGTCCACACCGGAGGGTGCTAGGGACTTTTTGGTACCGGCCAGAATTAGGGAACATAGCTTTTACGCTTT
GCCCCAAAGTCCACAAATTTATAAACAGTTATTAATGGTTGGTGGTACGGAAAAGTACTTCCAAATTGCCCATGTTTACC
GCGATGAAGACAGCCGCAAAGACCGTCAACCTGAACACACCCAAATTGACTTAGAGGTGGCCTTTTACACCAAGGAAATG
GTGATGGATTTAATCCAAAGGTTATTTGTCGATGTCTTTCGCCAAGTGTTAAACATTAAGCTCAAAAAACCGTTTCCAGT
GTTAAAGTTTGCTGAAGCCTTTAACCGTTTTGGCAGTGACAAACCGGATTTACGTTATGGTTTCGAACTGGAAGACTGCA
CTGATTTATTCCAAGACAGTCCGAACCAATTCACTAACTTAATTAATGCTGGCGGCATAGTCGGTGGGATCCAATTACCG
AATCTTTATTTAGACGAAGTTTCTTTTAAAGCATTGAGGAAACTAGCTAAGGACAACGGGGTTTCCTTAGAGTTTTACTC
TGATAAAGCTAGTTCCCTAAAACAACCCCTGGATTTACCGTTAGCAGGCACCATCTTACTGGTAGCACACAAAAGTAAAA
CGCAAGCTTGAACGGCATTAGGCGCCATTCGCAATGAGTTGAAGTACCACCTCAACTTAGTGAAACCCAACCAGTACAGC
TTTTGTTGAATTGTAGACTTTCCTTTATACGAGTTTGACGAAAAGGAACAGAAGTGGGTTTCGGCGCACAACATGTTCTC
CAATCCCCAACCGCAGTGGTTGGTTAACTTTGAAAACCACAAGGCTGAGGCGTTGAGTGAACAGTACGACCTAGTTTTAA
ATGGGTTTGAACTGGGTAGTGGTTCAATTCGAATCCATGACCCAGAAGTACAAACCCGTTTAATGCAATCTTTAGGGGTG
GATCCCCAACAGTTTGGCTTTGTGATGGAGGCTTACCAGTACGGTGCTCCTGTACACGCTGGCATGGGTTTGGGATTAGA
CCGCTTAATGATGATCATTAACAATGTGGACAACATTAGGGAAGTGATGGCCTTTCCGAAAAATGCCCAAGGGATAGAGA
TGCACACTAATGCCCCTGATCAGGTTGACATTAAAGACATAACAACAATATGGTCCAAACACCCAGTGAAATAA

Upstream 100 bases:

>100_bases
GGTTACCGCTTTTTAGGGGTCATTGGTGGTAATAATTTAGAACAGCAAACCATTACCGTTAAAGACCTGGCTACTGAACA
GCAAACAACATACACTTGAG

Downstream 100 bases:

>100_bases
ATACCCACCTTAAACACCTCTTGGCCTGTGATGCTTATAAGCTATCACACCGCTTAATGTATCCTAATGACACGACCAAC
TTATACAGTTGTTTAACAGC

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 557; Mature: 557

Protein sequence:

>557_residues
MNLDNAKCFKQRVFIGNLTSEHLNKTVTIAGWVKRIKKLGELNFVIVGDKTNTIQVTCKNKEQVKYLTREDLVIVKGKLK
RLDSVRFEITNPTITLFAKSKTPPLIIEDKTDALEEVRLRYRYLDLRRPVMQKRLALRHKVTLAVRNWLDQMGFIEVETP
TLTKSTPEGARDFLVPARIREHSFYALPQSPQIYKQLLMVGGTEKYFQIAHVYRDEDSRKDRQPEHTQIDLEVAFYTKEM
VMDLIQRLFVDVFRQVLNIKLKKPFPVLKFAEAFNRFGSDKPDLRYGFELEDCTDLFQDSPNQFTNLINAGGIVGGIQLP
NLYLDEVSFKALRKLAKDNGVSLEFYSDKASSLKQPLDLPLAGTILLVAHKSKTQAWTALGAIRNELKYHLNLVKPNQYS
FCWIVDFPLYEFDEKEQKWVSAHNMFSNPQPQWLVNFENHKAEALSEQYDLVLNGFELGSGSIRIHDPEVQTRLMQSLGV
DPQQFGFVMEAYQYGAPVHAGMGLGLDRLMMIINNVDNIREVMAFPKNAQGIEMHTNAPDQVDIKDITTIWSKHPVK

Sequences:

>Translated_557_residues
MNLDNAKCFKQRVFIGNLTSEHLNKTVTIAGWVKRIKKLGELNFVIVGDKTNTIQVTCKNKEQVKYLTREDLVIVKGKLK
RLDSVRFEITNPTITLFAKSKTPPLIIEDKTDALEEVRLRYRYLDLRRPVMQKRLALRHKVTLAVRN*LDQMGFIEVETP
TLTKSTPEGARDFLVPARIREHSFYALPQSPQIYKQLLMVGGTEKYFQIAHVYRDEDSRKDRQPEHTQIDLEVAFYTKEM
VMDLIQRLFVDVFRQVLNIKLKKPFPVLKFAEAFNRFGSDKPDLRYGFELEDCTDLFQDSPNQFTNLINAGGIVGGIQLP
NLYLDEVSFKALRKLAKDNGVSLEFYSDKASSLKQPLDLPLAGTILLVAHKSKTQA*TALGAIRNELKYHLNLVKPNQYS
FC*IVDFPLYEFDEKEQKWVSAHNMFSNPQPQWLVNFENHKAEALSEQYDLVLNGFELGSGSIRIHDPEVQTRLMQSLGV
DPQQFGFVMEAYQYGAPVHAGMGLGLDRLMMIINNVDNIREVMAFPKNAQGIEMHTNAPDQVDIKDITTIWSKHPVK
>Mature_557_residues
MNLDNAKCFKQRVFIGNLTSEHLNKTVTIAGWVKRIKKLGELNFVIVGDKTNTIQVTCKNKEQVKYLTREDLVIVKGKLK
RLDSVRFEITNPTITLFAKSKTPPLIIEDKTDALEEVRLRYRYLDLRRPVMQKRLALRHKVTLAVRN*LDQMGFIEVETP
TLTKSTPEGARDFLVPARIREHSFYALPQSPQIYKQLLMVGGTEKYFQIAHVYRDEDSRKDRQPEHTQIDLEVAFYTKEM
VMDLIQRLFVDVFRQVLNIKLKKPFPVLKFAEAFNRFGSDKPDLRYGFELEDCTDLFQDSPNQFTNLINAGGIVGGIQLP
NLYLDEVSFKALRKLAKDNGVSLEFYSDKASSLKQPLDLPLAGTILLVAHKSKTQA*TALGAIRNELKYHLNLVKPNQYS
FC*IVDFPLYEFDEKEQKWVSAHNMFSNPQPQWLVNFENHKAEALSEQYDLVLNGFELGSGSIRIHDPEVQTRLMQSLGV
DPQQFGFVMEAYQYGAPVHAGMGLGLDRLMMIINNVDNIREVMAFPKNAQGIEMHTNAPDQVDIKDITTIWSKHPVK

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=578, Percent_Identity=34.4290657439446, Blast_Score=302, Evalue=6e-82,
Organism=Homo sapiens, GI45439306, Length=272, Percent_Identity=24.6323529411765, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI5031815, Length=255, Percent_Identity=24.3137254901961, Blast_Score=66, Evalue=9e-11,
Organism=Escherichia coli, GI1788173, Length=573, Percent_Identity=35.9511343804538, Blast_Score=347, Evalue=8e-97,
Organism=Escherichia coli, GI1790571, Length=289, Percent_Identity=28.0276816608997, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1789256, Length=280, Percent_Identity=27.5, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI32566633, Length=559, Percent_Identity=31.8425760286225, Blast_Score=259, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI17551876, Length=266, Percent_Identity=26.3157894736842, Blast_Score=78, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71994340, Length=253, Percent_Identity=26.0869565217391, Blast_Score=68, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17535925, Length=253, Percent_Identity=26.0869565217391, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17535927, Length=253, Percent_Identity=26.0869565217391, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6325153, Length=290, Percent_Identity=38.2758620689655, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6323011, Length=307, Percent_Identity=28.3387622149837, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24584738, Length=572, Percent_Identity=33.5664335664336, Blast_Score=265, Evalue=7e-71,
Organism=Drosophila melanogaster, GI17136276, Length=273, Percent_Identity=26.7399267399267, Blast_Score=82, Evalue=1e-15,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 63502; Mature: 63502

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLDNAKCFKQRVFIGNLTSEHLNKTVTIAGWVKRIKKLGELNFVIVGDKTNTIQVTCKN
CCCCHHHHHHHHHEECCCCHHHCCCEEEHHHHHHHHHHHCCCEEEEECCCCCEEEEEECC
KEQVKYLTREDLVIVKGKLKRLDSVRFEITNPTITLFAKSKTPPLIIEDKTDALEEVRLR
HHHHHHHCCCCEEEEECHHHHHCCEEEEEECCEEEEEECCCCCCEEEECCHHHHHHHHHH
YRYLDLRRPVMQKRLALRHKVTLAVRNLDQMGFIEVETPTLTKSTPEGARDFLVPARIRE
HHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEECCCCCCCCCCCCHHHCCCCHHHCC
HSFYALPQSPQIYKQLLMVGGTEKYFQIAHVYRDEDSRKDRQPEHTQIDLEVAFYTKEMV
CCEEECCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEHHHHHH
MDLIQRLFVDVFRQVLNIKLKKPFPVLKFAEAFNRFGSDKPDLRYGFELEDCTDLFQDSP
HHHHHHHHHHHHHHHHCEECCCCCCHHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHCCC
NQFTNLINAGGIVGGIQLPNLYLDEVSFKALRKLAKDNGVSLEFYSDKASSLKQPLDLPL
HHHHHHHHCCCEEECEECCCHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCCCCCC
AGTILLVAHKSKTQATALGAIRNELKYHLNLVKPNQYSFCIVDFPLYEFDEKEQKWVSAH
CCEEEEEEECCCHHHHHHHHHHHHHHEEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHH
NMFSNPQPQWLVNFENHKAEALSEQYDLVLNGFELGSGSIRIHDPEVQTRLMQSLGVDPQ
HHCCCCCCCEEEEECCCHHHHHHHHHHEEEEEEEECCCEEEEECHHHHHHHHHHHCCCHH
QFGFVMEAYQYGAPVHAGMGLGLDRLMMIINNVDNIREVMAFPKNAQGIEMHTNAPDQVD
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHCCCCCCCEEEECCCCCCCC
IKDITTIWSKHPVK
HHHHHHHHHCCCCC
>Mature Secondary Structure
MNLDNAKCFKQRVFIGNLTSEHLNKTVTIAGWVKRIKKLGELNFVIVGDKTNTIQVTCKN
CCCCHHHHHHHHHEECCCCHHHCCCEEEHHHHHHHHHHHCCCEEEEECCCCCEEEEEECC
KEQVKYLTREDLVIVKGKLKRLDSVRFEITNPTITLFAKSKTPPLIIEDKTDALEEVRLR
HHHHHHHCCCCEEEEECHHHHHCCEEEEEECCEEEEEECCCCCCEEEECCHHHHHHHHHH
YRYLDLRRPVMQKRLALRHKVTLAVRNLDQMGFIEVETPTLTKSTPEGARDFLVPARIRE
HHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEECCCCCCCCCCCCHHHCCCCHHHCC
HSFYALPQSPQIYKQLLMVGGTEKYFQIAHVYRDEDSRKDRQPEHTQIDLEVAFYTKEMV
CCEEECCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEHHHHHH
MDLIQRLFVDVFRQVLNIKLKKPFPVLKFAEAFNRFGSDKPDLRYGFELEDCTDLFQDSP
HHHHHHHHHHHHHHHHCEECCCCCCHHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHCCC
NQFTNLINAGGIVGGIQLPNLYLDEVSFKALRKLAKDNGVSLEFYSDKASSLKQPLDLPL
HHHHHHHHCCCEEECEECCCHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCCCCCC
AGTILLVAHKSKTQATALGAIRNELKYHLNLVKPNQYSFCIVDFPLYEFDEKEQKWVSAH
CCEEEEEEECCCHHHHHHHHHHHHHHEEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHH
NMFSNPQPQWLVNFENHKAEALSEQYDLVLNGFELGSGSIRIHDPEVQTRLMQSLGVDPQ
HHCCCCCCCEEEEECCCHHHHHHHHHHEEEEEEEECCCEEEEECHHHHHHHHHHHCCCHH
QFGFVMEAYQYGAPVHAGMGLGLDRLMMIINNVDNIREVMAFPKNAQGIEMHTNAPDQVD
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHCCCCCCCEEEECCCCCCCC
IKDITTIWSKHPVK
HHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]