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The map label for this gene is glpF [H]

Identifier: 13507782

GI number: 13507782

Start: 51634

End: 52428

Strand: Direct

Name: glpF [H]

Synonym: MPN043

Alternate gene names: 13507782

Gene position: 51634-52428 (Clockwise)

Preceding gene: 13507781

Following gene: 13507784

Centisome position: 6.32

GC content: 40.0

Gene sequence:

>795_bases
ATGTTTAATTTAAGTGATTTTAGTGAATTACCACGTTGGATTGGTGCCGAATTTTTAGGCACATTCTTTTTAATTCTTTC
AGGTAACGGTGCTGGTTCGCAGCTAACACTCAATAAGATGTTTGCTAAAGAATCGAAAGCAAAGTTACTAACTGCTGCTT
TTGCTTGAGGTATTGCTGTATTAGTTGGTGTTTTAATAGCTAACTCTTTGTTTGAGGGTGCGGGTAATATTAACCCGGCA
GTGAGTTTGTTCTATGCTGTTTCGGGAACTATTCAAAAAGCTTTATATCCATTGCATGTAAACTTCAGCATTCCATTACT
TTGAGTAGCACTCCTACTTGCTTGAGTAGCACAGTTTGCTGGGGCAATGTTGGCACAAGCATTGTTAAACTTCTTGTTCT
GAAAGCATATTGAACAAACTGATCCACAATCGGTTTTGGTTACCCATTGTACTAACCCAGCAATCTTTAACATTCCTCGC
AACTTTGCAACTGAGTTTGTAGCAACTAGCGTTTTAATTGCTAGTTTACTGGTGGCTGGTAGTTTTGGTGCCAACCGATT
TGACCAGTCACCACGAGGGGTTGTACCAATGTTAGTGGTTACTGGCTTAATTATGTCTTTTGGTGCCGCTACTGGTACTG
CAATTAACCCAGCCCGTGATTTAGGTCCGAGAATTGTTTACTGATTGAGTCCAATTAAAAATAAGGATCCTAACTTAAAG
TACAGTTGGATTCCGGTAGCTGCACCTCTAAGTGCTAGTGTTATCTTAGGTGTGCTTGTGGCTGTTATCGTTTAG

Upstream 100 bases:

>100_bases
TCAGTTTTATTTTGAAAAATTCTAAATAAGAGATATAATTTTACTGATAGCTTTTCTTAAAAAATAACTTTTTGAGAGGG
CGAAAATTCTTTTTACTAAT

Downstream 100 bases:

>100_bases
TTCCTTAAAGCGTTCTTTAAACTGCTTGGTCTTAACTGTAACTGGCTTACCCTGCACTGGGTTGGTGCAACTTACAACGT
GGTTCATAAGCTTCAATATC

Product: glycerol uptake facilitator

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MFNLSDFSELPRWIGAEFLGTFFLILSGNGAGSQLTLNKMFAKESKAKLLTAAFAWGIAVLVGVLIANSLFEGAGNINPA
VSLFYAVSGTIQKALYPLHVNFSIPLLWVALLLAWVAQFAGAMLAQALLNFLFWKHIEQTDPQSVLVTHCTNPAIFNIPR
NFATEFVATSVLIASLLVAGSFGANRFDQSPRGVVPMLVVTGLIMSFGAATGTAINPARDLGPRIVYWLSPIKNKDPNLK
YSWIPVAAPLSASVILGVLVAVIV

Sequences:

>Translated_264_residues
MFNLSDFSELPRWIGAEFLGTFFLILSGNGAGSQLTLNKMFAKESKAKLLTAAFA*GIAVLVGVLIANSLFEGAGNINPA
VSLFYAVSGTIQKALYPLHVNFSIPLL*VALLLA*VAQFAGAMLAQALLNFLF*KHIEQTDPQSVLVTHCTNPAIFNIPR
NFATEFVATSVLIASLLVAGSFGANRFDQSPRGVVPMLVVTGLIMSFGAATGTAINPARDLGPRIVY*LSPIKNKDPNLK
YSWIPVAAPLSASVILGVLVAVIV
>Mature_264_residues
MFNLSDFSELPRWIGAEFLGTFFLILSGNGAGSQLTLNKMFAKESKAKLLTAAFA*GIAVLVGVLIANSLFEGAGNINPA
VSLFYAVSGTIQKALYPLHVNFSIPLL*VALLLA*VAQFAGAMLAQALLNFLF*KHIEQTDPQSVLVTHCTNPAIFNIPR
NFATEFVATSVLIASLLVAGSFGANRFDQSPRGVVPMLVVTGLIMSFGAATGTAINPARDLGPRIVY*LSPIKNKDPNLK
YSWIPVAAPLSASVILGVLVAVIV

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI22538420, Length=260, Percent_Identity=29.2307692307692, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI157266307, Length=270, Percent_Identity=27.7777777777778, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI71992966, Length=260, Percent_Identity=29.2307692307692, Blast_Score=66, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17531431, Length=256, Percent_Identity=28.90625, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17531429, Length=256, Percent_Identity=28.90625, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6321054, Length=221, Percent_Identity=28.5067873303167, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 27285; Mature: 27285

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNLSDFSELPRWIGAEFLGTFFLILSGNGAGSQLTLNKMFAKESKAKLLTAAFAGIAVL
CCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHH
VGVLIANSLFEGAGNINPAVSLFYAVSGTIQKALYPLHVNFSIPLLVALLLAVAQFAGAM
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHH
LAQALLNFLFKHIEQTDPQSVLVTHCTNPAIFNIPRNFATEFVATSVLIASLLVAGSFGA
HHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCC
NRFDQSPRGVVPMLVVTGLIMSFGAATGTAINPARDLGPRIVYLSPIKNKDPNLKYSWIP
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCCEEEEECCCCCCCCCCEEEEEE
VAAPLSASVILGVLVAVIV
CCCCHHHHHHHHHHHHHHC
>Mature Secondary Structure
MFNLSDFSELPRWIGAEFLGTFFLILSGNGAGSQLTLNKMFAKESKAKLLTAAFAGIAVL
CCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHH
VGVLIANSLFEGAGNINPAVSLFYAVSGTIQKALYPLHVNFSIPLLVALLLAVAQFAGAM
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHH
LAQALLNFLFKHIEQTDPQSVLVTHCTNPAIFNIPRNFATEFVATSVLIASLLVAGSFGA
HHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCC
NRFDQSPRGVVPMLVVTGLIMSFGAATGTAINPARDLGPRIVYLSPIKNKDPNLKYSWIP
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCCEEEEECCCCCCCCCCEEEEEE
VAAPLSASVILGVLVAVIV
CCCCHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]