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The map label for this gene is cbiF

Identifier: 15669774

GI number: 15669774

Start: 1554054

End: 1554833

Strand: Direct

Name: cbiF

Synonym: MJ1578

Alternate gene names: 15669774

Gene position: 1554054-1554833 (Clockwise)

Preceding gene: 15669773

Following gene: 15669777

Centisome position: 93.34

GC content: 34.62

Gene sequence:

>780_bases
ATGGATAATAATAGAAAAGTTATAATTGTTGGAGCAGGTCCTGGAGACCCTGAGCTAATAACAATCAAAGGTAAAAAAGC
TATAGAAGAGGCGGATGTAATTATCTATGCTGGTTCATTAGTCAATGAAAAGCTCTTAGAGTATAACAAAAAAAATGCTG
AAATTTACAACAGTGCAAACATGAACTTAGAGGAAATAATTGATGTGATGGTTAAAGCAGTTAATCAAGGAAAGAAAGTC
GTTAGATTACACACAGGAGACCCTTCTATTTACGGAGCTATAAAGGAGCAGATTGATGAGCTATCTAAATATGGAATAGA
TGTAGAGATAATTCCAGGAGTTAGCTCCTTATTTGCAGCAACTGCTTCATTAAAAGTTGAGCTAACTCTCCCAGAGGTTT
CTCAGACAGTTATTATCACAAGACCAGAGGGAAGGACTCCAATGCCAGAAAAAGAAAAGTTGAGGGATTTAGCTAAGCAT
CAATCAACAATGGCTATTTTTTTAGGCGTTTCAATGATTGACAAGGTTGTTAAAGAGCTAATTGAAGGTGGCTATAGAGA
AGAAACTCCAGTGGCTGTTGTTTATCACGCTTCATGGGATGATGAAAAAATAGTTAGAGGGACTTTAAAGGATATAGCTG
AAAAGGTAAAAAAAGAGGGAATTAAAAAAACTGCACTAATAATTGTTGGAGAGGTTTTAAATCCAAAGTATTATGCATAC
TCTAAACTCTACGATAAGAATTTTGAGCATGAATATAGAAAAAGCCATAAAAAATTTTGA

Upstream 100 bases:

>100_bases
AGGCTTTGGTGGCGGGGGAGGAGGAGCGAGATAATTTTCATAAGTAAATTTTATTAATTTTGAAAGACCTACTATAAAAA
CAAAAACAAGAGGAGAAATA

Downstream 100 bases:

>100_bases
TGAAACTTTTTTGAAAAGTTTCATTCTGACTTTTGATAAAGATTTTGAACATGAATACAGGAAAAAGAAGTAAGAAAATT
TTTATTTTTATTTTTTACTT

Product: cobalamin biosynthesis precorrin-3 methylase CbiF

Products: S-adenosyl-L-homocysteine; Precorrin 5

Alternate protein names: Cobalt-precorrin-3 methylase

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MDNNRKVIIVGAGPGDPELITIKGKKAIEEADVIIYAGSLVNEKLLEYNKKNAEIYNSANMNLEEIIDVMVKAVNQGKKV
VRLHTGDPSIYGAIKEQIDELSKYGIDVEIIPGVSSLFAATASLKVELTLPEVSQTVIITRPEGRTPMPEKEKLRDLAKH
QSTMAIFLGVSMIDKVVKELIEGGYREETPVAVVYHASWDDEKIVRGTLKDIAEKVKKEGIKKTALIIVGEVLNPKYYAY
SKLYDKNFEHEYRKSHKKF

Sequences:

>Translated_259_residues
MDNNRKVIIVGAGPGDPELITIKGKKAIEEADVIIYAGSLVNEKLLEYNKKNAEIYNSANMNLEEIIDVMVKAVNQGKKV
VRLHTGDPSIYGAIKEQIDELSKYGIDVEIIPGVSSLFAATASLKVELTLPEVSQTVIITRPEGRTPMPEKEKLRDLAKH
QSTMAIFLGVSMIDKVVKELIEGGYREETPVAVVYHASWDDEKIVRGTLKDIAEKVKKEGIKKTALIIVGEVLNPKYYAY
SKLYDKNFEHEYRKSHKKF
>Mature_259_residues
MDNNRKVIIVGAGPGDPELITIKGKKAIEEADVIIYAGSLVNEKLLEYNKKNAEIYNSANMNLEEIIDVMVKAVNQGKKV
VRLHTGDPSIYGAIKEQIDELSKYGIDVEIIPGVSSLFAATASLKVELTLPEVSQTVIITRPEGRTPMPEKEKLRDLAKH
QSTMAIFLGVSMIDKVVKELIEGGYREETPVAVVYHASWDDEKIVRGTLKDIAEKVKKEGIKKTALIIVGEVLNPKYYAY
SKLYDKNFEHEYRKSHKKF

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5

COG id: COG2875

COG function: function code H; Precorrin-4 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family

Homologues:

Organism=Escherichia coli, GI1789768, Length=241, Percent_Identity=29.8755186721992, Blast_Score=120, Evalue=9e-29,
Organism=Saccharomyces cerevisiae, GI6322922, Length=239, Percent_Identity=26.3598326359833, Blast_Score=93, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CBIF_METJA (Q58973)

Other databases:

- EMBL:   L77117
- PIR:   A64497
- RefSeq:   NP_248587.1
- ProteinModelPortal:   Q58973
- SMR:   Q58973
- GeneID:   1452487
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1578
- NMPDR:   fig|243232.1.peg.1624
- TIGR:   MJ1578
- HOGENOM:   HBG730212
- OMA:   TVIITRP
- ProtClustDB:   CLSK2750013
- BioCyc:   MJAN243232:MJ_1578-MONOMER
- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043
- Gene3D:   G3DSA:3.40.1010.10
- Gene3D:   G3DSA:3.30.950.10
- TIGRFAMs:   TIGR01465

Pfam domain/function: PF00590 TP_methylase; SSF53790 Cor/por_Metransf

EC number: 2.1.1.133

Molecular weight: Translated: 29004; Mature: 29004

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: PS00839 SUMT_1; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNNRKVIIVGAGPGDPELITIKGKKAIEEADVIIYAGSLVNEKLLEYNKKNAEIYNSAN
CCCCCEEEEEECCCCCCCEEEEECCCCCCCCCEEEEECHHHHHHHHHCCCCCCEEECCCC
MNLEEIIDVMVKAVNQGKKVVRLHTGDPSIYGAIKEQIDELSKYGIDVEIIPGVSSLFAA
CCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHH
TASLKVELTLPEVSQTVIITRPEGRTPMPEKEKLRDLAKHQSTMAIFLGVSMIDKVVKEL
HEEEEEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEGGYREETPVAVVYHASWDDEKIVRGTLKDIAEKVKKEGIKKTALIIVGEVLNPKYYAY
HCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEHHCCCCHHHH
SKLYDKNFEHEYRKSHKKF
HHHHCCCCCHHHHHHHCCC
>Mature Secondary Structure
MDNNRKVIIVGAGPGDPELITIKGKKAIEEADVIIYAGSLVNEKLLEYNKKNAEIYNSAN
CCCCCEEEEEECCCCCCCEEEEECCCCCCCCCEEEEECHHHHHHHHHCCCCCCEEECCCC
MNLEEIIDVMVKAVNQGKKVVRLHTGDPSIYGAIKEQIDELSKYGIDVEIIPGVSSLFAA
CCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHH
TASLKVELTLPEVSQTVIITRPEGRTPMPEKEKLRDLAKHQSTMAIFLGVSMIDKVVKEL
HEEEEEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEGGYREETPVAVVYHASWDDEKIVRGTLKDIAEKVKKEGIKKTALIIVGEVLNPKYYAY
HCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEHHCCCCHHHH
SKLYDKNFEHEYRKSHKKF
HHHHCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 4

Specific reaction: S-adenosyl-L-methionine + precorrin-4 = S-adenosyl-L-homocysteine + precorrin-5

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087