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The map label for this gene is pyrB

Identifier: 15669777

GI number: 15669777

Start: 1555753

End: 1556673

Strand: Direct

Name: pyrB

Synonym: MJ1581

Alternate gene names: 15669777

Gene position: 1555753-1556673 (Clockwise)

Preceding gene: 15669774

Following gene: 15669778

Centisome position: 93.44

GC content: 32.57

Gene sequence:

>921_bases
ATGAAGCATCTAATATCAATGAAGGATATTGGAAAAGAGGAGATTTTAGAGATTTTAGATGAAGCAAGAAAGATGGAAGA
GCTTTTAAATACAAAAAGACCTTTAAAGTTATTGGAAGGGAAAATATTAGCAACTGTTTTTTATGAACCATCAACAAGGA
CAAGATTGAGTTTTGAAACGGCAATGAAGAGGTTAGGTGGAGAAGTAATAACAATGACTGATTTAAAAAGCTCTTCTGTT
GCAAAGGGAGAGAGTTTAATAGATACAATTAGAGTAATTAGTGGATATGCTGATATAATTGTTTTAAGGCATCCAAGTGA
AGGAGCTGCAAGATTGGCAAGTGAATATTCTCAAGTTCCAATTATAAATGCTGGGGATGGGAGTAATCAGCATCCTACTC
AAACTCTTTTGGATTTATACACAATAATGAGAGAGATTGGCAGGATAGATGGGATAAAGATAGCGTTTGTTGGAGATTTG
AAGTATGGAAGGACAGTTCATTCCTTGGTCTATGCCCTATCCTTATTTGAAAATGTTGAGATGTATTTTGTATCTCCAAA
AGAATTGAGACTACCAAAAGATATTATTGAAGATTTAAAAGCTAAAAATATAAAATTTTATGAAAAAGAAAGTTTGGATG
ATTTAGATGATGATATAGATGTTCTATATGTAACAAGAATCCAGAAGGAGAGGTTTCCAGACCCTAACGAATATGAAAAG
GTTAAAGGTAGCTATAAGATAAAGAGAGAGTATGTCGAAGGAAAGAAGTTTATAATTATGCATCCATTACCAAGAGTTGA
TGAAATTGATTATGATGTTGATGATTTACCTCAAGCAAAGTATTTTAAGCAGAGTTTTTATGGAATTCCAGTGAGAATGG
CCATTTTAAAGAAGCTAATTGAGGATAATGAAGGTGAATAG

Upstream 100 bases:

>100_bases
ATTATCTTGAAAGACACACAATATTTTCTCCAAAATTTTTATAAATCTTTTTTATAAATACTCTATCAAACCTCATATAT
ATGCTTTAATAGGGATAATT

Downstream 100 bases:

>100_bases
TTTTGAGAGATGTGAAATATGTTGATGCTCACTGCCATATAGAAGATAAAGCATTCAACAAAAACAGAGATGAGGTCATT
GAGAGGGCTAAGAAAGAAGA

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MKHLISMKDIGKEEILEILDEARKMEELLNTKRPLKLLEGKILATVFYEPSTRTRLSFETAMKRLGGEVITMTDLKSSSV
AKGESLIDTIRVISGYADIIVLRHPSEGAARLASEYSQVPIINAGDGSNQHPTQTLLDLYTIMREIGRIDGIKIAFVGDL
KYGRTVHSLVYALSLFENVEMYFVSPKELRLPKDIIEDLKAKNIKFYEKESLDDLDDDIDVLYVTRIQKERFPDPNEYEK
VKGSYKIKREYVEGKKFIIMHPLPRVDEIDYDVDDLPQAKYFKQSFYGIPVRMAILKKLIEDNEGE

Sequences:

>Translated_306_residues
MKHLISMKDIGKEEILEILDEARKMEELLNTKRPLKLLEGKILATVFYEPSTRTRLSFETAMKRLGGEVITMTDLKSSSV
AKGESLIDTIRVISGYADIIVLRHPSEGAARLASEYSQVPIINAGDGSNQHPTQTLLDLYTIMREIGRIDGIKIAFVGDL
KYGRTVHSLVYALSLFENVEMYFVSPKELRLPKDIIEDLKAKNIKFYEKESLDDLDDDIDVLYVTRIQKERFPDPNEYEK
VKGSYKIKREYVEGKKFIIMHPLPRVDEIDYDVDDLPQAKYFKQSFYGIPVRMAILKKLIEDNEGE
>Mature_306_residues
MKHLISMKDIGKEEILEILDEARKMEELLNTKRPLKLLEGKILATVFYEPSTRTRLSFETAMKRLGGEVITMTDLKSSSV
AKGESLIDTIRVISGYADIIVLRHPSEGAARLASEYSQVPIINAGDGSNQHPTQTLLDLYTIMREIGRIDGIKIAFVGDL
KYGRTVHSLVYALSLFENVEMYFVSPKELRLPKDIIEDLKAKNIKFYEKESLDDLDDDIDVLYVTRIQKERFPDPNEYEK
VKGSYKIKREYVEGKKFIIMHPLPRVDEIDYDVDDLPQAKYFKQSFYGIPVRMAILKKLIEDNEGE

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=301, Percent_Identity=43.1893687707641, Blast_Score=269, Evalue=2e-72,
Organism=Homo sapiens, GI38788445, Length=313, Percent_Identity=27.1565495207668, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI2367364, Length=299, Percent_Identity=49.1638795986622, Blast_Score=292, Evalue=1e-80,
Organism=Escherichia coli, GI1790703, Length=202, Percent_Identity=28.2178217821782, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1786469, Length=202, Percent_Identity=26.7326732673267, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI193204318, Length=319, Percent_Identity=45.141065830721, Blast_Score=244, Evalue=5e-65,
Organism=Saccharomyces cerevisiae, GI6322331, Length=302, Percent_Identity=43.7086092715232, Blast_Score=251, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6322373, Length=294, Percent_Identity=26.1904761904762, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24642586, Length=302, Percent_Identity=45.364238410596, Blast_Score=273, Evalue=1e-73,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_METJA (Q58976)

Other databases:

- EMBL:   L77117
- PIR:   D64497
- RefSeq:   NP_248590.1
- PDB:   2RGW
- PDB:   3E2P
- PDBsum:   2RGW
- PDBsum:   3E2P
- ProteinModelPortal:   Q58976
- SMR:   Q58976
- GeneID:   1452490
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1581
- NMPDR:   fig|243232.1.peg.1627
- TIGR:   MJ1581
- HOGENOM:   HBG579429
- OMA:   FKQSFYG
- ProtClustDB:   PRK00856
- BioCyc:   MJAN243232:MJ_1581-MONOMER
- BRENDA:   2.1.3.2
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 35160; Mature: 35160

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHLISMKDIGKEEILEILDEARKMEELLNTKRPLKLLEGKILATVFYEPSTRTRLSFET
CCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCEEEEEEEECCCCCCCHHHHH
AMKRLGGEVITMTDLKSSSVAKGESLIDTIRVISGYADIIVLRHPSEGAARLASEYSQVP
HHHHHCCCEEEEECCCCCCHHCHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHCCC
IINAGDGSNQHPTQTLLDLYTIMREIGRIDGIKIAFVGDLKYGRTVHSLVYALSLFENVE
EEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCE
MYFVSPKELRLPKDIIEDLKAKNIKFYEKESLDDLDDDIDVLYVTRIQKERFPDPNEYEK
EEEECHHHHCCCHHHHHHHHHCCCEEECCCCCCCCCCCCCEEEEEHHHHHCCCCCCHHHH
VKGSYKIKREYVEGKKFIIMHPLPRVDEIDYDVDDLPQAKYFKQSFYGIPVRMAILKKLI
HCCCHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHH
EDNEGE
CCCCCC
>Mature Secondary Structure
MKHLISMKDIGKEEILEILDEARKMEELLNTKRPLKLLEGKILATVFYEPSTRTRLSFET
CCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCEEEEEEEECCCCCCCHHHHH
AMKRLGGEVITMTDLKSSSVAKGESLIDTIRVISGYADIIVLRHPSEGAARLASEYSQVP
HHHHHCCCEEEEECCCCCCHHCHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHCCC
IINAGDGSNQHPTQTLLDLYTIMREIGRIDGIKIAFVGDLKYGRTVHSLVYALSLFENVE
EEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCE
MYFVSPKELRLPKDIIEDLKAKNIKFYEKESLDDLDDDIDVLYVTRIQKERFPDPNEYEK
EEEECHHHHCCCHHHHHHHHHCCCEEECCCCCCCCCCCCCEEEEEHHHHHCCCCCCHHHH
VKGSYKIKREYVEGKKFIIMHPLPRVDEIDYDVDDLPQAKYFKQSFYGIPVRMAILKKLI
HCCCHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHH
EDNEGE
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087; 10748118; 10944354