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The map label for this gene is cofH

Identifier: 15669622

GI number: 15669622

Start: 1401317

End: 1402396

Strand: Direct

Name: cofH

Synonym: MJ1431

Alternate gene names: 15669622

Gene position: 1401317-1402396 (Clockwise)

Preceding gene: 15669621

Following gene: 15669623

Centisome position: 84.16

GC content: 34.26

Gene sequence:

>1080_bases
ATGGACCCAAATAAATTTAGAGAGAAAGAAATATCCAAAAAAGAAGCATTAGAGTTATTTGAAGATAATGAGATTATATT
TGAGTTGTTTAAATTTGCAGATTCTTTGAGAAGAGAGGAAGTTGGAGATATAGTTACCTACGTGGTAAATAGAAATATAA
ACTTCACAAATATATGCGTTGGAAATTGCAGATTTTGTGCTTTCAGAGCCAATGAAAATGACAAACATGCTTATTTTTTA
GATATAGATGAGATTGCTAAGAGGGCAGTAGAAGCAAAAAAATTTGGTTGCACTGAGGTTTGTATTCAGGGAGGATTGCA
TCCAAAGATAGATACATATTATCAGGCGGAGATTTTAAAGGCTGTGCATGAAGCAACAAAACCTTACGGAGATATCCACA
TACATGCCTTTTCTCCAATGGAGGTTTATTTTGGTGCTGAGAATGCTGGCTTAGATATTAAAGAAGCATTGAAAATATTG
AAAGAAAATGGTCTCAACTCCATGCCAGGAACTGCGGCGGAGATTTTAGATGATGACATTAGAGCTGAACTCTGCCCAAA
TAAAATAAAAACTAAAGAGTGGATTTATATAATTAAGGAGGCTCATAAATTAGGCATTCCAACAACTGCAACAATGATGT
ATGGGCATATCGAAGAATATAAACACTGGGTAAATCATCTTTTTATAATTAAAGAGATTCAGGAAGAGACTAACGGCTTT
ACTGAATTTGTTCCACTCTCATTTATGCATAAATATGCTCCAATCTATAAAGAAGGAAAGGCAAAAGCTGGAGCTACTGG
AATTGAAGATTTAAAGGTTTTTGCTGTTAGCAGAATAATATTTAAGGGTTTGATAAAAAATATCCAAGCTTCATGGGTTA
AATTAGGAAAAAAGATGGTTCAAGTTGCTTTAAGATGTGGGGCTAACGATGTTGGTGGCACTTTGATAGAGGAGAGTATA
TCAAGAAGTGCAGGAGCTGAGCATGGAGTTTATATGAGTGTTGAAGAGATTAGAGACATGATTAAAAGGGTTGGATTAAT
TCCTAAGGAGAGAACTACTTTGTATAAAATTTTAGAGTAA

Upstream 100 bases:

>100_bases
GGGGTTGCATGTCATGAGGGTTATTATTCATGTTTCCATTATAAAATAGAAGATGGAGAACTAAAAATAACTGGAGAATA
CTACTCTAAGAGATGAGAAT

Downstream 100 bases:

>100_bases
TTTATTATAAAAGGTGAGAGGTTGGAAATAAGGAAAAGATATTTTTTAAGTAAAAAAGATGTTAAAAAAATAAAAAAAGA
ATTGGAAGTGTTTTTTGAAA

Product: FO synthase subunit 2

Products: NA

Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MDPNKFREKEISKKEALELFEDNEIIFELFKFADSLRREEVGDIVTYVVNRNINFTNICVGNCRFCAFRANENDKHAYFL
DIDEIAKRAVEAKKFGCTEVCIQGGLHPKIDTYYQAEILKAVHEATKPYGDIHIHAFSPMEVYFGAENAGLDIKEALKIL
KENGLNSMPGTAAEILDDDIRAELCPNKIKTKEWIYIIKEAHKLGIPTTATMMYGHIEEYKHWVNHLFIIKEIQEETNGF
TEFVPLSFMHKYAPIYKEGKAKAGATGIEDLKVFAVSRIIFKGLIKNIQASWVKLGKKMVQVALRCGANDVGGTLIEESI
SRSAGAEHGVYMSVEEIRDMIKRVGLIPKERTTLYKILE

Sequences:

>Translated_359_residues
MDPNKFREKEISKKEALELFEDNEIIFELFKFADSLRREEVGDIVTYVVNRNINFTNICVGNCRFCAFRANENDKHAYFL
DIDEIAKRAVEAKKFGCTEVCIQGGLHPKIDTYYQAEILKAVHEATKPYGDIHIHAFSPMEVYFGAENAGLDIKEALKIL
KENGLNSMPGTAAEILDDDIRAELCPNKIKTKEWIYIIKEAHKLGIPTTATMMYGHIEEYKHWVNHLFIIKEIQEETNGF
TEFVPLSFMHKYAPIYKEGKAKAGATGIEDLKVFAVSRIIFKGLIKNIQASWVKLGKKMVQVALRCGANDVGGTLIEESI
SRSAGAEHGVYMSVEEIRDMIKRVGLIPKERTTLYKILE
>Mature_359_residues
MDPNKFREKEISKKEALELFEDNEIIFELFKFADSLRREEVGDIVTYVVNRNINFTNICVGNCRFCAFRANENDKHAYFL
DIDEIAKRAVEAKKFGCTEVCIQGGLHPKIDTYYQAEILKAVHEATKPYGDIHIHAFSPMEVYFGAENAGLDIKEALKIL
KENGLNSMPGTAAEILDDDIRAELCPNKIKTKEWIYIIKEAHKLGIPTTATMMYGHIEEYKHWVNHLFIIKEIQEETNGF
TEFVPLSFMHKYAPIYKEGKAKAGATGIEDLKVFAVSRIIFKGLIKNIQASWVKLGKKMVQVALRCGANDVGGTLIEESI
SRSAGAEHGVYMSVEEIRDMIKRVGLIPKERTTLYKILE

Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO)

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. CofH family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COFH_METJA (Q58826)

Other databases:

- EMBL:   L77117
- PIR:   F64478
- RefSeq:   NP_248435.1
- ProteinModelPortal:   Q58826
- GeneID:   1452335
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1431
- NMPDR:   fig|243232.1.peg.1472
- TIGR:   MJ1431
- HOGENOM:   HBG312134
- OMA:   TMMYGHI
- ProtClustDB:   PRK07360
- BioCyc:   MetaCyc:MONOMER-12179
- BioCyc:   MJAN243232:MJ_1431-MONOMER
- HAMAP:   MF_01612
- InterPro:   IPR013785
- InterPro:   IPR019940
- InterPro:   IPR006638
- InterPro:   IPR020050
- InterPro:   IPR005244
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF004762
- SMART:   SM00729
- TIGRFAMs:   TIGR00423
- TIGRFAMs:   TIGR03551

Pfam domain/function: PF04055 Radical_SAM

EC number: NA

Molecular weight: Translated: 40807; Mature: 40807

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPNKFREKEISKKEALELFEDNEIIFELFKFADSLRREEVGDIVTYVVNRNINFTNICV
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
GNCRFCAFRANENDKHAYFLDIDEIAKRAVEAKKFGCTEVCIQGGLHPKIDTYYQAEILK
CCCEEEEEECCCCCCEEEEEEHHHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHH
AVHEATKPYGDIHIHAFSPMEVYFGAENAGLDIKEALKILKENGLNSMPGTAAEILDDDI
HHHHHHCCCCCEEEEEECCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHH
RAELCPNKIKTKEWIYIIKEAHKLGIPTTATMMYGHIEEYKHWVNHLFIIKEIQEETNGF
HHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
TEFVPLSFMHKYAPIYKEGKAKAGATGIEDLKVFAVSRIIFKGLIKNIQASWVKLGKKMV
HHHHHHHHHHHHCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVALRCGANDVGGTLIEESISRSAGAEHGVYMSVEEIRDMIKRVGLIPKERTTLYKILE
HHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHCCCCCCHHHHHHHCC
>Mature Secondary Structure
MDPNKFREKEISKKEALELFEDNEIIFELFKFADSLRREEVGDIVTYVVNRNINFTNICV
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
GNCRFCAFRANENDKHAYFLDIDEIAKRAVEAKKFGCTEVCIQGGLHPKIDTYYQAEILK
CCCEEEEEECCCCCCEEEEEEHHHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHH
AVHEATKPYGDIHIHAFSPMEVYFGAENAGLDIKEALKILKENGLNSMPGTAAEILDDDI
HHHHHHCCCCCEEEEEECCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHH
RAELCPNKIKTKEWIYIIKEAHKLGIPTTATMMYGHIEEYKHWVNHLFIIKEIQEETNGF
HHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
TEFVPLSFMHKYAPIYKEGKAKAGATGIEDLKVFAVSRIIFKGLIKNIQASWVKLGKKMV
HHHHHHHHHHHHCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVALRCGANDVGGTLIEESISRSAGAEHGVYMSVEEIRDMIKRVGLIPKERTTLYKILE
HHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087