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The map label for this gene is pyrE

Identifier: 15669297

GI number: 15669297

Start: 1050484

End: 1051014

Strand: Direct

Name: pyrE

Synonym: MJ1109

Alternate gene names: 15669297

Gene position: 1050484-1051014 (Clockwise)

Preceding gene: 15669290

Following gene: 15669304

Centisome position: 63.09

GC content: 33.71

Gene sequence:

>531_bases
ATGGACAAAAAATCCAAATTAATAAACCTGCTAAAAGAGGTTGGTTGTATAAGATTTGGAGAATTTATCTTAGCCTCTGG
TAAAAAAAGTAACTACTACATAGACATAAAAAAAGCCACCACAAACCCAGAAATTTTAAAGTTAGTTGGAGAAATTATTG
CTGAGCAAATAAAGGATGAAGATGTAAAAGTTGCTGGAGTAGAGCTTGGTTCTGTCCCTATAGCTACAGCTGTCTCAATT
ATTGCTCAAAAACCACTATTAATTGTTAGAAAGAAACCTAAGGATTACGGAACTAAAAATAAGATAGAAGGAGAGCTAAA
AGAAGGAGATAAGGTTGTTATTGTGGAGGATGTTACTACAACTGGAGGAAGTGTGCTAAAGGCAGTTAAAGAGATTAGGG
AAAATGGTGGAATTGTTGATAAAGTTTTTGTTGTTGTTGATAGGTTAGAAGGAGCTAAAGAAAACCTACAAAAAGAGAAT
GTTGAATTAATCCCATTAGTTACTGTTAAGGAGCTACAATCCACTCAATAA

Upstream 100 bases:

>100_bases
AGACAAAACTTTTTTTATCTTAAGCTACAGAATTATAGAAACTTTTTTAAAAGAATTAAACAAATCTTAAAGAGCAGAGT
ATATACAATTTGGTGATATT

Downstream 100 bases:

>100_bases
ATCTAAAAACCTCTTAGTCCATAGGGGAAACCCCTATTGGGATACTCCCCGTCCATTAAGTTGCTCCTTTCAGGAGCAAT
TAATGTCCATTTTAAGCTTA

Product: orotate phosphoribosyl transferase PyrE

Products: NA

Alternate protein names: OPRT; OPRTase

Number of amino acids: Translated: 176; Mature: 176

Protein sequence:

>176_residues
MDKKSKLINLLKEVGCIRFGEFILASGKKSNYYIDIKKATTNPEILKLVGEIIAEQIKDEDVKVAGVELGSVPIATAVSI
IAQKPLLIVRKKPKDYGTKNKIEGELKEGDKVVIVEDVTTTGGSVLKAVKEIRENGGIVDKVFVVVDRLEGAKENLQKEN
VELIPLVTVKELQSTQ

Sequences:

>Translated_176_residues
MDKKSKLINLLKEVGCIRFGEFILASGKKSNYYIDIKKATTNPEILKLVGEIIAEQIKDEDVKVAGVELGSVPIATAVSI
IAQKPLLIVRKKPKDYGTKNKIEGELKEGDKVVIVEDVTTTGGSVLKAVKEIRENGGIVDKVFVVVDRLEGAKENLQKEN
VELIPLVTVKELQSTQ
>Mature_176_residues
MDKKSKLINLLKEVGCIRFGEFILASGKKSNYYIDIKKATTNPEILKLVGEIIAEQIKDEDVKVAGVELGSVPIATAVSI
IAQKPLLIVRKKPKDYGTKNKIEGELKEGDKVVIVEDVTTTGGSVLKAVKEIRENGGIVDKVFVVVDRLEGAKENLQKEN
VELIPLVTVKELQSTQ

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily

Homologues:

Organism=Homo sapiens, GI4507835, Length=169, Percent_Identity=34.9112426035503, Blast_Score=112, Evalue=1e-25,
Organism=Escherichia coli, GI1790073, Length=172, Percent_Identity=28.4883720930233, Blast_Score=72, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17554964, Length=169, Percent_Identity=38.4615384615385, Blast_Score=117, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17508631, Length=165, Percent_Identity=35.7575757575758, Blast_Score=117, Evalue=3e-27,
Organism=Drosophila melanogaster, GI17933654, Length=177, Percent_Identity=36.1581920903955, Blast_Score=104, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRE_METJA (Q58509)

Other databases:

- EMBL:   L77117
- PIR:   D64438
- RefSeq:   NP_248102.1
- ProteinModelPortal:   Q58509
- SMR:   Q58509
- GeneID:   1452006
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1109
- NMPDR:   fig|243232.1.peg.1139
- TIGR:   MJ1109
- HOGENOM:   HBG404341
- OMA:   GKEADYY
- ProtClustDB:   CLSK2749958
- BioCyc:   MJAN243232:MJ_1109-MONOMER
- BRENDA:   2.4.2.10
- HAMAP:   MF_01208
- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836
- TIGRFAMs:   TIGR00336

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.4.2.10

Molecular weight: Translated: 19284; Mature: 19284

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: BINDING 90-90 BINDING 91-91 BINDING 94-94 BINDING 120-120 BINDING 148-148

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKKSKLINLLKEVGCIRFGEFILASGKKSNYYIDIKKATTNPEILKLVGEIIAEQIKDE
CCHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCC
DVKVAGVELGSVPIATAVSIIAQKPLLIVRKKPKDYGTKNKIEGELKEGDKVVIVEDVTT
CEEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCC
TGGSVLKAVKEIRENGGIVDKVFVVVDRLEGAKENLQKENVELIPLVTVKELQSTQ
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHCCCEEEEEEEHHHHCCCC
>Mature Secondary Structure
MDKKSKLINLLKEVGCIRFGEFILASGKKSNYYIDIKKATTNPEILKLVGEIIAEQIKDE
CCHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCC
DVKVAGVELGSVPIATAVSIIAQKPLLIVRKKPKDYGTKNKIEGELKEGDKVVIVEDVTT
CEEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCC
TGGSVLKAVKEIRENGGIVDKVFVVVDRLEGAKENLQKENVELIPLVTVKELQSTQ
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHCCCEEEEEEEHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087