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The map label for this gene is glmU

Identifier: 15669289

GI number: 15669289

Start: 1041901

End: 1043127

Strand: Direct

Name: glmU

Synonym: MJ1101

Alternate gene names: 15669289

Gene position: 1041901-1043127 (Clockwise)

Preceding gene: 15669288

Following gene: 15669290

Centisome position: 62.58

GC content: 30.32

Gene sequence:

>1227_bases
ATGGATGCCATAATATTATGTGCAGGGAAAGGGGAGAGATTAAGACCTCTAACAGAGAACAGACCAAAACCAATGATTCC
TATAGCTGGAAAGCCAATTTTACAACATATTATTGAAAAAGTTGAGGATTTGGTAGATAATATTTACTTAATTGTTAAGT
ATAAAAAAGAAAAGATTGTTGATTATTTTAAAAACCATCCAAAAATCAAATTTTTAGAGCAGGGAGAAATAGATGGAACT
GGACAGGCAGTTTTAACAGCCAAGGATTATGTAGATGATGAATTTTTAGTTATAAATGGGGATATTATCTTTGAAGATGA
CTTAGAAGAATTTTTAAAATACAAATATGCTGTTGCTGTTAAAGAGGTAAAAAATCCAGAAAACTTTGGAGTTGTAGTTT
TAGATGATGAAAATAATATTATAGAACTCCAAGAAAAGCCAGAAAACCCAAAATCAAATTTAATAAATGCCGGAATATAC
AAATTTGACAAAAAGATTTTTGAATTAATTGAAAAAACAAAGATTTCTGAAAGAGGAGAGAGAGAACTTACAGATGCAAT
AAAACATCTTATTAAAGAAGAAAAAGTTAAAGGAATTAAGTTAAATGGTTATTGGAACGATGTTGGAAGACCATGGGACA
TTTTGGAGGCAAATAAATATCTCCTGGATAAAATAAATACAGATATCAAGGGGAAAATTGAAGAAAATGTTGTTATTAAA
GGAGAGGTTATAATAGAAGAGGGAGCAATTGTTAAAGCAAATTCAGTTATTGAAGGGCCTGCAATTATTAAAAAAGGGGC
TGTTGTGGGGCCGTTAGCTTATATAAGACCATATACTGTTTTAATGGAAAATACTTTTGTTGGAAATTCATCTGAAGTTA
AGGCAAGTATAATTATGAAAAATACAAAAATTCCACATCTATCTTATGTTGGAGATAGTATAATTGGAGAGAACTGCAAT
TTTGGTTGCAATACAATAACTGCCAACTTAAGATTTGATGATAAACCAGTTAAAGTCAATATAAAGAGTAAAAGGGTTGA
GAGCGTTAGAAAATTGGGAGTTATAATGGGAGATAATGTTAAAACAGGTATTCAAGTCTCTTTTATGCCAGGGGTTAAAG
TTGGAAGTAACTGCTGGATTGGGGCAAGTTGTTTAATTGATAGAGATATAGAAAGTAATACATTTGTTTATAAAAGAGAT
GAGCTGATATTTAGAAAATTGAAATAG

Upstream 100 bases:

>100_bases
AAATAACAAAGATGCTAAAGAGTTGTTAGAAAAAGGGATAAAGTTAGTTAAAGAAGCTTTAAGTGCTTTATAAAACTATT
TTTAAATTTTGGTGATATAA

Downstream 100 bases:

>100_bases
AAGAGTGTTATGCCCTATATTGGTATATCTCCAAGAGACATTAAAAGGGGCTGTAAGCCCCTTCTTAATGTATCCCGGGT
ATACCAATAGGGCAGAGCCC

Product: glucose-1-phosphate thymidylyltransferase StrD

Products: NA

Alternate protein names: UDP-N-acetylglucosamine pyrophosphorylase; N-acetylglucosamine-1-phosphate uridyltransferase; Glucosamine-1-phosphate N-acetyltransferase

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MDAIILCAGKGERLRPLTENRPKPMIPIAGKPILQHIIEKVEDLVDNIYLIVKYKKEKIVDYFKNHPKIKFLEQGEIDGT
GQAVLTAKDYVDDEFLVINGDIIFEDDLEEFLKYKYAVAVKEVKNPENFGVVVLDDENNIIELQEKPENPKSNLINAGIY
KFDKKIFELIEKTKISERGERELTDAIKHLIKEEKVKGIKLNGYWNDVGRPWDILEANKYLLDKINTDIKGKIEENVVIK
GEVIIEEGAIVKANSVIEGPAIIKKGAVVGPLAYIRPYTVLMENTFVGNSSEVKASIIMKNTKIPHLSYVGDSIIGENCN
FGCNTITANLRFDDKPVKVNIKSKRVESVRKLGVIMGDNVKTGIQVSFMPGVKVGSNCWIGASCLIDRDIESNTFVYKRD
ELIFRKLK

Sequences:

>Translated_408_residues
MDAIILCAGKGERLRPLTENRPKPMIPIAGKPILQHIIEKVEDLVDNIYLIVKYKKEKIVDYFKNHPKIKFLEQGEIDGT
GQAVLTAKDYVDDEFLVINGDIIFEDDLEEFLKYKYAVAVKEVKNPENFGVVVLDDENNIIELQEKPENPKSNLINAGIY
KFDKKIFELIEKTKISERGERELTDAIKHLIKEEKVKGIKLNGYWNDVGRPWDILEANKYLLDKINTDIKGKIEENVVIK
GEVIIEEGAIVKANSVIEGPAIIKKGAVVGPLAYIRPYTVLMENTFVGNSSEVKASIIMKNTKIPHLSYVGDSIIGENCN
FGCNTITANLRFDDKPVKVNIKSKRVESVRKLGVIMGDNVKTGIQVSFMPGVKVGSNCWIGASCLIDRDIESNTFVYKRD
ELIFRKLK
>Mature_408_residues
MDAIILCAGKGERLRPLTENRPKPMIPIAGKPILQHIIEKVEDLVDNIYLIVKYKKEKIVDYFKNHPKIKFLEQGEIDGT
GQAVLTAKDYVDDEFLVINGDIIFEDDLEEFLKYKYAVAVKEVKNPENFGVVVLDDENNIIELQEKPENPKSNLINAGIY
KFDKKIFELIEKTKISERGERELTDAIKHLIKEEKVKGIKLNGYWNDVGRPWDILEANKYLLDKINTDIKGKIEENVVIK
GEVIIEEGAIVKANSVIEGPAIIKKGAVVGPLAYIRPYTVLMENTFVGNSSEVKASIIMKNTKIPHLSYVGDSIIGENCN
FGCNTITANLRFDDKPVKVNIKSKRVESVRKLGVIMGDNVKTGIQVSFMPGVKVGSNCWIGASCLIDRDIESNTFVYKRD
ELIFRKLK

Specific function: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetyl-glucosamine (UDP- GlcNAc). Responsible for the acetylation of GlcN-1-P to GlcNAc-1- P, and for the uridyl transfer from UTP to GlcNAc-1-P, to produce UDP-GlcN

COG id: COG1208

COG function: function code MJ; Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transferase hexapeptide repeat family

Homologues:

Organism=Homo sapiens, GI11761621, Length=339, Percent_Identity=27.7286135693215, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI11761619, Length=339, Percent_Identity=27.7286135693215, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI31881779, Length=361, Percent_Identity=21.0526315789474, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI45447090, Length=361, Percent_Identity=21.0526315789474, Blast_Score=79, Evalue=7e-15,
Organism=Escherichia coli, GI1790168, Length=457, Percent_Identity=26.2582056892779, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1790224, Length=240, Percent_Identity=30.8333333333333, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1788351, Length=235, Percent_Identity=27.2340425531915, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1787488, Length=291, Percent_Identity=27.1477663230241, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI133931050, Length=343, Percent_Identity=30.3206997084548, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17509979, Length=336, Percent_Identity=25, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17509981, Length=327, Percent_Identity=24.4648318042813, Blast_Score=83, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6320148, Length=357, Percent_Identity=29.1316526610644, Blast_Score=126, Evalue=6e-30,
Organism=Drosophila melanogaster, GI21355443, Length=335, Percent_Identity=27.1641791044776, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24644084, Length=335, Percent_Identity=27.1641791044776, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24653912, Length=385, Percent_Identity=22.3376623376623, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLMU_METJA (Q58501)

Other databases:

- EMBL:   L77117
- PIR:   D64437
- RefSeq:   NP_248094.1
- ProteinModelPortal:   Q58501
- SMR:   Q58501
- GeneID:   1451998
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1101
- NMPDR:   fig|243232.1.peg.1131
- TIGR:   MJ1101
- HOGENOM:   HBG688195
- OMA:   GPNCFIR
- ProtClustDB:   CLSK876450
- BioCyc:   MJAN243232:MJ_1101-MONOMER
- InterPro:   IPR005835
- InterPro:   IPR011004

Pfam domain/function: PF00483 NTP_transferase; SSF51161 Trimer_LpxA_like

EC number: =2.7.7.23; =2.3.1.157

Molecular weight: Translated: 46056; Mature: 46056

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: ACT_SITE 306-306 BINDING 74-74 BINDING 79-79 BINDING 80-80 BINDING 130-130 BINDING 142-142 BINDING 156-156 BINDING 382-382 BINDING 399-399

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAIILCAGKGERLRPLTENRPKPMIPIAGKPILQHIIEKVEDLVDNIYLIVKYKKEKIV
CCEEEEECCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCEEEEEEECHHHHH
DYFKNHPKIKFLEQGEIDGTGQAVLTAKDYVDDEFLVINGDIIFEDDLEEFLKYKYAVAV
HHHHCCCCEEEEECCCCCCCCCEEEEEHHCCCCEEEEEECCEEEHHHHHHHHHHHHHHHH
KEVKNPENFGVVVLDDENNIIELQEKPENPKSNLINAGIYKFDKKIFELIEKTKISERGE
HHCCCCCCCEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
RELTDAIKHLIKEEKVKGIKLNGYWNDVGRPWDILEANKYLLDKINTDIKGKIEENVVIK
HHHHHHHHHHHHHHHCCEEEEECEECCCCCCCCHHHCCHHHHHHCCCCCCCEECCCEEEE
GEVIIEEGAIVKANSVIEGPAIIKKGAVVGPLAYIRPYTVLMENTFVGNSSEVKASIIMK
EEEEEECCCEEEECCEECCCHHHCCCCEECCHHHHCCEEEEEEEEECCCCCCEEEEEEEE
NTKIPHLSYVGDSIIGENCNFGCNTITANLRFDDKPVKVNIKSKRVESVRKLGVIMGDNV
CCCCCCHHHCCCHHCCCCCCCCCCEEEEEEEECCCCEEEEEHHHHHHHHHHCCEEECCCC
KTGIQVSFMPGVKVGSNCWIGASCLIDRDIESNTFVYKRDELIFRKLK
CCCEEEEEECCCEECCCCEECEEEEEECCCCCCEEEEECHHHHHHCCC
>Mature Secondary Structure
MDAIILCAGKGERLRPLTENRPKPMIPIAGKPILQHIIEKVEDLVDNIYLIVKYKKEKIV
CCEEEEECCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCEEEEEEECHHHHH
DYFKNHPKIKFLEQGEIDGTGQAVLTAKDYVDDEFLVINGDIIFEDDLEEFLKYKYAVAV
HHHHCCCCEEEEECCCCCCCCCEEEEEHHCCCCEEEEEECCEEEHHHHHHHHHHHHHHHH
KEVKNPENFGVVVLDDENNIIELQEKPENPKSNLINAGIYKFDKKIFELIEKTKISERGE
HHCCCCCCCEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
RELTDAIKHLIKEEKVKGIKLNGYWNDVGRPWDILEANKYLLDKINTDIKGKIEENVVIK
HHHHHHHHHHHHHHHCCEEEEECEECCCCCCCCHHHCCHHHHHHCCCCCCCEECCCEEEE
GEVIIEEGAIVKANSVIEGPAIIKKGAVVGPLAYIRPYTVLMENTFVGNSSEVKASIIMK
EEEEEECCCEEEECCEECCCHHHCCCCEECCHHHHCCEEEEEEEEECCCCCCEEEEEEEE
NTKIPHLSYVGDSIIGENCNFGCNTITANLRFDDKPVKVNIKSKRVESVRKLGVIMGDNV
CCCCCCHHHCCCHHCCCCCCCCCCEEEEEEEECCCCEEEEEHHHHHHHHHHCCEEECCCC
KTGIQVSFMPGVKVGSNCWIGASCLIDRDIESNTFVYKRDELIFRKLK
CCCEEEEEECCCEECCCCEECEEEEEECCCCCCEEEEECHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087