Click here to switch to the map view.

The map label for this gene is cobA [H]

Identifier: 15669155

GI number: 15669155

Start: 899093

End: 899854

Strand: Direct

Name: cobA [H]

Synonym: MJ0965

Alternate gene names: 15669155

Gene position: 899093-899854 (Clockwise)

Preceding gene: 15669152

Following gene: 15669156

Centisome position: 54.0

GC content: 34.51

Gene sequence:

>762_bases
ATGCCAAAAAATATAAACATTTCTGGTGAAATAATGACAGGCAAAGTTATCTTAGTAGGAGCAGGACCGGGAGACCCAGA
GTTGATAACAATAAAAGGTTTAAAAGCTATTAAAGAGGCAGATGTTGTTGTTTATGATGATTTAATATCAAAAGAGCTAT
TAAATTATGCTAAAAAAGATGCCGAGCTAATTTATGTTGGTAAAAGAAAGGGAAAACATTCATTTAAACAAGAAGAGATT
AATAAAATATTGGTTGAGAAGGCAAAGGAAGGAAAGTTAGTTGTTAGATTAAAAGGTGGAGACCCATTTGTTTTTGGTAG
AGGTGGAGAAGAGATTTTAGAGTTAAAGAAGCATAACATACCTTATGAGGTAATTCCGGGAATAACGTCAGCTATAGCAG
TCCCAGAGGTTGCTGGGATTCCAGTTACTCATAGAAAGGTAGCTACCTCCTTTACAGTAGTTACAGGGCATGAGGCAGAG
GATAAGAAAGAGAAACAGGTAGATTTAAGCAAGTTAAATGCTGATACTATTGTAATTTTAATGGGAATAACTAATTTGGA
AAATTTGGTTAAAGAGTTGTTGCAAAACCCAAAAAGAAGTAAAGAAACACCAGTAGCAATCATTATGGAGGGAACTACAA
AGAATCAGAGAGTTATAAAAGGGACTTTGGGAGATATTGTTGAAAAGGCTAAAAAAGAAAATGCAAGACCTCCAGGAGTT
ATAGTTGTTGGTGAGGTTGTTAATGTGTTAGATAGTCAATAA

Upstream 100 bases:

>100_bases
AAATAAACGAGGTCTGTAAATGTCCCACCTATATCAATTCCTACTCTGTATCCCATAACCATCCCATAAAAATTAGTATT
AGAAGCTATAACTTATTTTA

Downstream 100 bases:

>100_bases
ATTAATTTTTGTGATTAAAATGATAAAGAATGTTGCTATAATCTACCCAAACAAATTTAAAGCTGGGATTTCTTGCTTAG
CTGTGCATGTTCTAGCTAAT

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM [H]

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MPKNINISGEIMTGKVILVGAGPGDPELITIKGLKAIKEADVVVYDDLISKELLNYAKKDAELIYVGKRKGKHSFKQEEI
NKILVEKAKEGKLVVRLKGGDPFVFGRGGEEILELKKHNIPYEVIPGITSAIAVPEVAGIPVTHRKVATSFTVVTGHEAE
DKKEKQVDLSKLNADTIVILMGITNLENLVKELLQNPKRSKETPVAIIMEGTTKNQRVIKGTLGDIVEKAKKENARPPGV
IVVGEVVNVLDSQ

Sequences:

>Translated_253_residues
MPKNINISGEIMTGKVILVGAGPGDPELITIKGLKAIKEADVVVYDDLISKELLNYAKKDAELIYVGKRKGKHSFKQEEI
NKILVEKAKEGKLVVRLKGGDPFVFGRGGEEILELKKHNIPYEVIPGITSAIAVPEVAGIPVTHRKVATSFTVVTGHEAE
DKKEKQVDLSKLNADTIVILMGITNLENLVKELLQNPKRSKETPVAIIMEGTTKNQRVIKGTLGDIVEKAKKENARPPGV
IVVGEVVNVLDSQ
>Mature_252_residues
PKNINISGEIMTGKVILVGAGPGDPELITIKGLKAIKEADVVVYDDLISKELLNYAKKDAELIYVGKRKGKHSFKQEEIN
KILVEKAKEGKLVVRLKGGDPFVFGRGGEEILELKKHNIPYEVIPGITSAIAVPEVAGIPVTHRKVATSFTVVTGHEAED
KKEKQVDLSKLNADTIVILMGITNLENLVKELLQNPKRSKETPVAIIMEGTTKNQRVIKGTLGDIVEKAKKENARPPGVI
VVGEVVNVLDSQ

Specific function: Catalyzes both methylations at C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=240, Percent_Identity=47.0833333333333, Blast_Score=219, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6322922, Length=237, Percent_Identity=35.4430379746835, Blast_Score=134, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 27538; Mature: 27406

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKNINISGEIMTGKVILVGAGPGDPELITIKGLKAIKEADVVVYDDLISKELLNYAKKD
CCCCCCCCEEEEEEEEEEEECCCCCCCEEEECCCHHHHHCCEEEEHHHHHHHHHHHHHCC
AELIYVGKRKGKHSFKQEEINKILVEKAKEGKLVVRLKGGDPFVFGRGGEEILELKKHNI
CEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCEEEECCCCHHHHHHHHCCC
PYEVIPGITSAIAVPEVAGIPVTHRKVATSFTVVTGHEAEDKKEKQVDLSKLNADTIVIL
CHHHHCCCHHHHCCCCCCCCCCCHHHHHEEEEEEECCCCCCHHHHHHHHHHCCCCEEEEE
MGITNLENLVKELLQNPKRSKETPVAIIMEGTTKNQRVIKGTLGDIVEKAKKENARPPGV
EECCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEEHHHHHHHHHHHHHHCCCCCCE
IVVGEVVNVLDSQ
EEHHHHHHHHCCC
>Mature Secondary Structure 
PKNINISGEIMTGKVILVGAGPGDPELITIKGLKAIKEADVVVYDDLISKELLNYAKKD
CCCCCCCEEEEEEEEEEEECCCCCCCEEEECCCHHHHHCCEEEEHHHHHHHHHHHHHCC
AELIYVGKRKGKHSFKQEEINKILVEKAKEGKLVVRLKGGDPFVFGRGGEEILELKKHNI
CEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCEEEECCCCHHHHHHHHCCC
PYEVIPGITSAIAVPEVAGIPVTHRKVATSFTVVTGHEAEDKKEKQVDLSKLNADTIVIL
CHHHHCCCHHHHCCCCCCCCCCCHHHHHEEEEEEECCCCCCHHHHHHHHHHCCCCEEEEE
MGITNLENLVKELLQNPKRSKETPVAIIMEGTTKNQRVIKGTLGDIVEKAKKENARPPGV
EECCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEEHHHHHHHHHHHHHHCCCCCCE
IVVGEVVNVLDSQ
EEHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]