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The map label for this gene is radA

Identifier: 15669060

GI number: 15669060

Start: 791860

End: 792918

Strand: Direct

Name: radA

Synonym: MJ0869

Alternate gene names: 15669060

Gene position: 791860-792918 (Clockwise)

Preceding gene: 15669057

Following gene: 15669062

Centisome position: 47.56

GC content: 36.26

Gene sequence:

>1059_bases
ATGATAACCTTCATATATTTCTTTGGCAATATCATCTATTACCTCCCAATTTATTCATATATTAACAATTACAAATTTAT
GCTTATGGTGATAATAATGGATGATTTAACTCAACTACCTGGTGTAGGTCCTACAACAGCTGAGAAGTTAAAAGAAGCTG
GTTACACTGATTTTATGAAAATTGCAACTGCATCCATCGGTGAGCTAACAGAAATAGATGGAATTAGTGAGAAAGCTGCA
GCCAGGATTATAGAAGCTGCAAGAGAACTCTGCAATTTAGGGTTTAAAAGTGGAACTGAGGTTTTATCCCAAAGAAAAAA
TATATGGAAGCTCTCAACTGGAAGTAAGAACTTAGATGAGATTTTAGGAGGAGGCTTGGAGAGTCAGTCAGTTACTGAAT
TTGCTGGAATGTTTGGTTCTGGTAAAACCCAGATAGCTCATCAGGCATGTGTTAATTTGCAGTGCCCAGAGAGAATAGTA
GCAGATGACGCAATAAAGGATGAGATTTTAAATGAACCAAAGGCAGTTTATATTGACACAGAAGGAACATTCAGACCAGA
AAGAATTGTCCAAATGGCAGAAGCTTTGGGTTTAGATGGAAATGAAGTTTTAAATAACATCTTTGTAGCAAGAGCCTACA
ACTCAGATATGCAAATGTTGTATGCTGAAAATGTTGAGAATTTAATAAGAGAAGGGCATAATATAAAGTTGGTTATAGTT
GATTCATTAACATCAACATTCAGAACTGAGTATATAGGAAGAGGTAAATTAGCAGAGAGACAACAAAAATTAGGAAGGCA
CATGGCTACTCTCAACAAATTAGCTGATATATACAACTGTGTTGTTATAGTAACTAACCAAGTAGCTGCAAGACCAGATG
CTTTATTTGGACCTTCAGAGCAGGCAATTGGAGGGCATATTGTTGGGCATGCAGCAACATTTAGAATATTCCTAAGAAAG
GCAAAAGGAGATAAGAGGGTTGCTAAGCTTTATGATTCTCCACACTTACCAGATGCTGAAGCAATGTTTAGGATAACCGA
AAAGGGAATTCATGATTAA

Upstream 100 bases:

>100_bases
TATATTTACTATTCTTAGGTTTTTATAAATTCTCTTCTTTTTGAAACATACTGTAGTTATAAACCAAAATCTTTATATAG
TCAAATATGACAATTGATGT

Downstream 100 bases:

>100_bases
TTCTTTAAATTAATTTTTAAAATATATCTTTAAAAAATAAAAGAAATAAAACATCTAAAAAATTAATTAGCAGATTTCTT
TTTTCATCAACTCTTTTACT

Product: DNA repair and recombination protein RadA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MITFIYFFGNIIYYLPIYSYINNYKFMLMVIIMDDLTQLPGVGPTTAEKLKEAGYTDFMKIATASIGELTEIDGISEKAA
ARIIEAARELCNLGFKSGTEVLSQRKNIWKLSTGSKNLDEILGGGLESQSVTEFAGMFGSGKTQIAHQACVNLQCPERIV
ADDAIKDEILNEPKAVYIDTEGTFRPERIVQMAEALGLDGNEVLNNIFVARAYNSDMQMLYAENVENLIREGHNIKLVIV
DSLTSTFRTEYIGRGKLAERQQKLGRHMATLNKLADIYNCVVIVTNQVAARPDALFGPSEQAIGGHIVGHAATFRIFLRK
AKGDKRVAKLYDSPHLPDAEAMFRITEKGIHD

Sequences:

>Translated_352_residues
MITFIYFFGNIIYYLPIYSYINNYKFMLMVIIMDDLTQLPGVGPTTAEKLKEAGYTDFMKIATASIGELTEIDGISEKAA
ARIIEAARELCNLGFKSGTEVLSQRKNIWKLSTGSKNLDEILGGGLESQSVTEFAGMFGSGKTQIAHQACVNLQCPERIV
ADDAIKDEILNEPKAVYIDTEGTFRPERIVQMAEALGLDGNEVLNNIFVARAYNSDMQMLYAENVENLIREGHNIKLVIV
DSLTSTFRTEYIGRGKLAERQQKLGRHMATLNKLADIYNCVVIVTNQVAARPDALFGPSEQAIGGHIVGHAATFRIFLRK
AKGDKRVAKLYDSPHLPDAEAMFRITEKGIHD
>Mature_352_residues
MITFIYFFGNIIYYLPIYSYINNYKFMLMVIIMDDLTQLPGVGPTTAEKLKEAGYTDFMKIATASIGELTEIDGISEKAA
ARIIEAARELCNLGFKSGTEVLSQRKNIWKLSTGSKNLDEILGGGLESQSVTEFAGMFGSGKTQIAHQACVNLQCPERIV
ADDAIKDEILNEPKAVYIDTEGTFRPERIVQMAEALGLDGNEVLNNIFVARAYNSDMQMLYAENVENLIREGHNIKLVIV
DSLTSTFRTEYIGRGKLAERQQKLGRHMATLNKLADIYNCVVIVTNQVAARPDALFGPSEQAIGGHIVGHAATFRIFLRK
AKGDKRVAKLYDSPHLPDAEAMFRITEKGIHD

Specific function: Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules

COG id: COG0468

COG function: function code L; RecA/RadA recombinase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic recA-like protein family

Homologues:

Organism=Homo sapiens, GI19924133, Length=315, Percent_Identity=43.8095238095238, Blast_Score=251, Evalue=6e-67,
Organism=Homo sapiens, GI23238219, Length=322, Percent_Identity=40.6832298136646, Blast_Score=234, Evalue=9e-62,
Organism=Homo sapiens, GI256017143, Length=317, Percent_Identity=40.6940063091483, Blast_Score=217, Evalue=1e-56,
Organism=Homo sapiens, GI256017141, Length=317, Percent_Identity=40.6940063091483, Blast_Score=217, Evalue=1e-56,
Organism=Homo sapiens, GI256017145, Length=236, Percent_Identity=42.7966101694915, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI17402896, Length=358, Percent_Identity=29.8882681564246, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI10835029, Length=318, Percent_Identity=27.3584905660377, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI46255039, Length=329, Percent_Identity=27.0516717325228, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI19924117, Length=318, Percent_Identity=27.3584905660377, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI153946430, Length=268, Percent_Identity=26.865671641791, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI4885659, Length=268, Percent_Identity=26.865671641791, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI153946427, Length=268, Percent_Identity=26.865671641791, Blast_Score=74, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71997301, Length=308, Percent_Identity=44.1558441558442, Blast_Score=244, Evalue=7e-65,
Organism=Caenorhabditis elegans, GI71997295, Length=308, Percent_Identity=44.1558441558442, Blast_Score=243, Evalue=8e-65,
Organism=Saccharomyces cerevisiae, GI6321027, Length=327, Percent_Identity=43.1192660550459, Blast_Score=255, Evalue=8e-69,
Organism=Saccharomyces cerevisiae, GI6320942, Length=318, Percent_Identity=40.251572327044, Blast_Score=214, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6320207, Length=201, Percent_Identity=30.3482587064677, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17864108, Length=323, Percent_Identity=40.8668730650155, Blast_Score=227, Evalue=9e-60,
Organism=Drosophila melanogaster, GI24651285, Length=283, Percent_Identity=42.756183745583, Blast_Score=221, Evalue=7e-58,
Organism=Drosophila melanogaster, GI17136506, Length=259, Percent_Identity=30.1158301158301, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24650551, Length=191, Percent_Identity=27.7486910994764, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RADA_METJA (Q49593)

Other databases:

- EMBL:   U45312
- EMBL:   L77117
- PIR:   E64408
- RefSeq:   NP_247864.1
- ProteinModelPortal:   Q49593
- SMR:   Q49593
- GeneID:   1451758
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0869
- NMPDR:   fig|243232.1.peg.901
- TIGR:   MJ0869
- HOGENOM:   HBG413235
- OMA:   FVARAYN
- ProtClustDB:   PRK04301
- BioCyc:   MJAN243232:MJ_0869-MONOMER
- HAMAP:   MF_00348
- InterPro:   IPR003593
- InterPro:   IPR013632
- InterPro:   IPR011938
- InterPro:   IPR016467
- InterPro:   IPR020588
- InterPro:   IPR010995
- InterPro:   IPR003583
- InterPro:   IPR020587
- PIRSF:   PIRSF005856
- SMART:   SM00382
- SMART:   SM00278
- TIGRFAMs:   TIGR02236

Pfam domain/function: PF08423 Rad51; SSF47794 Rad51_N

EC number: 3.6.1.-

Molecular weight: Translated: 39084; Mature: 39084

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS50162 RECA_2; PS50163 RECA_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITFIYFFGNIIYYLPIYSYINNYKFMLMVIIMDDLTQLPGVGPTTAEKLKEAGYTDFMK
CEEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHH
IATASIGELTEIDGISEKAAARIIEAARELCNLGFKSGTEVLSQRKNIWKLSTGSKNLDE
HHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCEEEECCCCCCHHH
ILGGGLESQSVTEFAGMFGSGKTQIAHQACVNLQCPERIVADDAIKDEILNEPKAVYIDT
HHCCCCCCCHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEEC
EGTFRPERIVQMAEALGLDGNEVLNNIFVARAYNSDMQMLYAENVENLIREGHNIKLVIV
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHCCCCEEEEEE
DSLTSTFRTEYIGRGKLAERQQKLGRHMATLNKLADIYNCVVIVTNQVAARPDALFGPSE
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHCCCCCCCCCCC
QAIGGHIVGHAATFRIFLRKAKGDKRVAKLYDSPHLPDAEAMFRITEKGIHD
CCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MITFIYFFGNIIYYLPIYSYINNYKFMLMVIIMDDLTQLPGVGPTTAEKLKEAGYTDFMK
CEEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHH
IATASIGELTEIDGISEKAAARIIEAARELCNLGFKSGTEVLSQRKNIWKLSTGSKNLDE
HHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCEEEECCCCCCHHH
ILGGGLESQSVTEFAGMFGSGKTQIAHQACVNLQCPERIVADDAIKDEILNEPKAVYIDT
HHCCCCCCCHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEEC
EGTFRPERIVQMAEALGLDGNEVLNNIFVARAYNSDMQMLYAENVENLIREGHNIKLVIV
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHCCCCEEEEEE
DSLTSTFRTEYIGRGKLAERQQKLGRHMATLNKLADIYNCVVIVTNQVAARPDALFGPSE
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHCCCCCCCCCCC
QAIGGHIVGHAATFRIFLRKAKGDKRVAKLYDSPHLPDAEAMFRITEKGIHD
CCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8668545; 8688087