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The map label for this gene is hyaD [C]

Identifier: 15668428

GI number: 15668428

Start: 239492

End: 239956

Strand: Direct

Name: hyaD [C]

Synonym: MJ0253

Alternate gene names: 15668428

Gene position: 239492-239956 (Clockwise)

Preceding gene: 15668427

Following gene: 15668429

Centisome position: 14.38

GC content: 33.33

Gene sequence:

>465_bases
ATGAAAAAGAAGGATATATTAATTGTTGGTTGTGGAAATCTGTTGTTTGGAGATGATGGCTTTGGATGTGAGGTTGTTAG
CAAATTAGAAAAAATGAACTTGCCAGATAACGTTGAAGTTATAGATGCTGGGGCAAGTGGAGCATACTATTTAATGACGT
TGGTTGATGAAGATATAAAAAAGATTATTGTTGTTGATGCCATTGATTTTGATTTAGAACCTGGAACAATAAAAAAGATA
GATGTTGATGAATTACCGAATATTAAAAAATATTCTTTCGATGCCCATAACGTTCCATTAGCCCCATTTCTCAAAGATTT
ACATAATAAAGGCATAGAAGTCGTGGTTATTGGTTGTCAAGGTAAAGAATTTACAATGCCAGACATAAAACCAGGGTTGT
CTGAAGAGGTTGCCAAGGCAGTTGATAAAGCAATAGAAATAATTCTGGGAGAAATTAAGAAATAA

Upstream 100 bases:

>100_bases
GATGAGAATGATTACGTCATTGTTGGAAGGGCAATCTATCAATCACAAAATCCTAAAGAAGAGGCTAAAAAATATAAAGA
AATGCTAAATAAGTGATAAA

Downstream 100 bases:

>100_bases
GGGATTTACATGCTAAAAGAGATTTTATTGGGAAATGCTGAAAAAGGGATAATAACTCAAATTTATGGGCCTCCAGGCGT
AGGGAAGACAAATATATGTA

Product: coenzyme F420-reducing hydrogenase subunit delta

Products: reduced coenzyme F420

Alternate protein names: NA

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MKKKDILIVGCGNLLFGDDGFGCEVVSKLEKMNLPDNVEVIDAGASGAYYLMTLVDEDIKKIIVVDAIDFDLEPGTIKKI
DVDELPNIKKYSFDAHNVPLAPFLKDLHNKGIEVVVIGCQGKEFTMPDIKPGLSEEVAKAVDKAIEIILGEIKK

Sequences:

>Translated_154_residues
MKKKDILIVGCGNLLFGDDGFGCEVVSKLEKMNLPDNVEVIDAGASGAYYLMTLVDEDIKKIIVVDAIDFDLEPGTIKKI
DVDELPNIKKYSFDAHNVPLAPFLKDLHNKGIEVVVIGCQGKEFTMPDIKPGLSEEVAKAVDKAIEIILGEIKK
>Mature_154_residues
MKKKDILIVGCGNLLFGDDGFGCEVVSKLEKMNLPDNVEVIDAGASGAYYLMTLVDEDIKKIIVVDAIDFDLEPGTIKKI
DVDELPNIKKYSFDAHNVPLAPFLKDLHNKGIEVVVIGCQGKEFTMPDIKPGLSEEVAKAVDKAIEIILGEIKK

Specific function: Protease Involved In The C-Terminal Processing Of Hyab, The Large Subunit Of Hydrogenase 1 (Potential). [C]

COG id: COG0680

COG function: function code C; Ni,Fe-hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A31 family

Homologues:

Organism=Escherichia coli, GI1787209, Length=87, Percent_Identity=34.4827586206897, Blast_Score=67, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y253_METJA (Q57701)

Other databases:

- EMBL:   L77117
- PIR:   F64331
- RefSeq:   NP_247224.1
- ProteinModelPortal:   Q57701
- SMR:   Q57701
- GeneID:   1451107
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0253
- NMPDR:   fig|243232.1.peg.261
- TIGR:   MJ0253
- HOGENOM:   HBG730505
- OMA:   KYTFDAH
- ProtClustDB:   CLSK2749860
- BioCyc:   MJAN243232:MJ_0253-MONOMER
- InterPro:   IPR004411
- InterPro:   IPR000671
- Gene3D:   G3DSA:3.40.50.1450
- PRINTS:   PR00446
- TIGRFAMs:   TIGR00130
- TIGRFAMs:   TIGR00072

Pfam domain/function: PF01750 HycI

EC number: 1.12.98.1

Molecular weight: Translated: 16846; Mature: 16846

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKDILIVGCGNLLFGDDGFGCEVVSKLEKMNLPDNVEVIDAGASGAYYLMTLVDEDIK
CCCCCEEEEECCCEEECCCCCCHHHHHHHHHCCCCCCCEEEECCCCCEEEEEEEHHHHHH
KIIVVDAIDFDLEPGTIKKIDVDELPNIKKYSFDAHNVPLAPFLKDLHNKGIEVVVIGCQ
EEEEEEEEECCCCCCCEEEECHHHCCCCCEECCCCCCCCHHHHHHHHHCCCCEEEEEECC
GKEFTMPDIKPGLSEEVAKAVDKAIEIILGEIKK
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKKDILIVGCGNLLFGDDGFGCEVVSKLEKMNLPDNVEVIDAGASGAYYLMTLVDEDIK
CCCCCEEEEECCCEEECCCCCCHHHHHHHHHCCCCCCCEEEECCCCCEEEEEEEHHHHHH
KIIVVDAIDFDLEPGTIKKIDVDELPNIKKYSFDAHNVPLAPFLKDLHNKGIEVVVIGCQ
EEEEEEEEECCCCCCCEEEECHHHCCCCCEECCCCCCCCHHHHHHHHHCCCCEEEEEECC
GKEFTMPDIKPGLSEEVAKAVDKAIEIILGEIKK
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2; coenzyme F420

Specific reaction: H2 + coenzyme F420 = reduced coenzyme F420

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087