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The map label for this gene is radB

Identifier: 15668429

GI number: 15668429

Start: 239966

End: 240604

Strand: Direct

Name: radB

Synonym: MJ0254

Alternate gene names: 15668429

Gene position: 239966-240604 (Clockwise)

Preceding gene: 15668428

Following gene: 15668431

Centisome position: 14.41

GC content: 31.3

Gene sequence:

>639_bases
ATGCTAAAAGAGATTTTATTGGGAAATGCTGAAAAAGGGATAATAACTCAAATTTATGGGCCTCCAGGCGTAGGGAAGAC
AAATATATGTATTATCAACTCTATAAACGCTGTTAATTCTGGGAAGGTAATTTATATAGACACTGAAGGGGGTTTATCAA
TAGAGAGGATTAAACAAATAGCCTCAAATAATTACAAAATAGTTTTAGAAAATATGATTATATACAATGCCTTTGATTTC
TATGAGCAGGATAAAATTATACAAAAAGAGCTTCCATTAATAACCAATAATGCAAGTTTGATAGTGGTTGATAACATAAC
ATCTTTATATAGATTAGAGTTGAGTGATGAAGCTAATAAAAACATCATGCTCAATAAAATGCTCGGCAACCAAGTAAAAA
CTTTATTAAAATTAGCTAAAACAAATAACTTAGCTGTTATAATAACAAATCAAGTAAGAGAGACTGTTAATGGCTTTGAG
GCTTCTGGAGGGAGGTTGTTAGAGTATTGGAGTAAATGTATTGTTAGGTTGGAAAAGCTCAACGGAGATAGATTGGCTAT
ATTGGAGAAACATCTACATGCTGGAGAGGAAAGAGTTAAATTTAGAATAGTTGAGAGAGGGATTGAAATTATTGATTAA

Upstream 100 bases:

>100_bases
AATGCCAGACATAAAACCAGGGTTGTCTGAAGAGGTTGCCAAGGCAGTTGATAAAGCAATAGAAATAATTCTGGGAGAAA
TTAAGAAATAAGGGATTTAC

Downstream 100 bases:

>100_bases
ACCTTTCCAAATGTGTCTCCTCTAAGACCTCTTCTCAATGTTTCCAAGCTTATAACCTCATCAAATGCAATATTTGCCAG
ATTAACTGAACTACCAAACT

Product: DNA repair and recombination protein RadB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MLKEILLGNAEKGIITQIYGPPGVGKTNICIINSINAVNSGKVIYIDTEGGLSIERIKQIASNNYKIVLENMIIYNAFDF
YEQDKIIQKELPLITNNASLIVVDNITSLYRLELSDEANKNIMLNKMLGNQVKTLLKLAKTNNLAVIITNQVRETVNGFE
ASGGRLLEYWSKCIVRLEKLNGDRLAILEKHLHAGEERVKFRIVERGIEIID

Sequences:

>Translated_212_residues
MLKEILLGNAEKGIITQIYGPPGVGKTNICIINSINAVNSGKVIYIDTEGGLSIERIKQIASNNYKIVLENMIIYNAFDF
YEQDKIIQKELPLITNNASLIVVDNITSLYRLELSDEANKNIMLNKMLGNQVKTLLKLAKTNNLAVIITNQVRETVNGFE
ASGGRLLEYWSKCIVRLEKLNGDRLAILEKHLHAGEERVKFRIVERGIEIID
>Mature_212_residues
MLKEILLGNAEKGIITQIYGPPGVGKTNICIINSINAVNSGKVIYIDTEGGLSIERIKQIASNNYKIVLENMIIYNAFDF
YEQDKIIQKELPLITNNASLIVVDNITSLYRLELSDEANKNIMLNKMLGNQVKTLLKLAKTNNLAVIITNQVRETVNGFE
ASGGRLLEYWSKCIVRLEKLNGDRLAILEKHLHAGEERVKFRIVERGIEIID

Specific function: Involved in DNA repair and in homologous recombination. May regulate the cleavage reactions of the branch-structured DNA. Has a very weak ATPase activity that is not stimulated by DNA. Binds DNA but does not promote DNA strands exchange

COG id: COG0468

COG function: function code L; RecA/RadA recombinase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic recA-like protein family. RadB subfamily

Homologues:

Organism=Homo sapiens, GI19924117, Length=185, Percent_Identity=31.3513513513514, Blast_Score=85, Evalue=5e-17,
Organism=Homo sapiens, GI46255039, Length=185, Percent_Identity=31.3513513513514, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI10835029, Length=185, Percent_Identity=30.8108108108108, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI19924133, Length=204, Percent_Identity=30.3921568627451, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI256017143, Length=199, Percent_Identity=30.6532663316583, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI256017141, Length=199, Percent_Identity=30.6532663316583, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71997301, Length=234, Percent_Identity=29.9145299145299, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71997295, Length=234, Percent_Identity=29.9145299145299, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321027, Length=234, Percent_Identity=31.1965811965812, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24651285, Length=229, Percent_Identity=30.5676855895196, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17864108, Length=229, Percent_Identity=30.5676855895196, Blast_Score=76, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RADB_METJA (Q57702)

Other databases:

- EMBL:   L77117
- PIR:   G64331
- RefSeq:   NP_247225.1
- ProteinModelPortal:   Q57702
- SMR:   Q57702
- GeneID:   1451108
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0254
- NMPDR:   fig|243232.1.peg.262
- TIGR:   MJ0254
- HOGENOM:   HBG413235
- OMA:   ITQIYGP
- ProtClustDB:   PRK09361
- BioCyc:   MJAN243232:MJ_0254-MONOMER
- HAMAP:   MF_00350
- InterPro:   IPR003593
- InterPro:   IPR013632
- InterPro:   IPR011939
- InterPro:   IPR020588
- PANTHER:   PTHR22942:SF9
- PIRSF:   PIRSF003336
- SMART:   SM00382
- TIGRFAMs:   TIGR02237

Pfam domain/function: PF08423 Rad51

EC number: NA

Molecular weight: Translated: 23842; Mature: 23842

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKEILLGNAEKGIITQIYGPPGVGKTNICIINSINAVNSGKVIYIDTEGGLSIERIKQI
CCCHHHCCCCCCCEEEEEECCCCCCCCCEEEEECCCCCCCCEEEEEECCCCCCHHHHHHH
ASNNYKIVLENMIIYNAFDFYEQDKIIQKELPLITNNASLIVVDNITSLYRLELSDEANK
HCCCCEEEEEEEEEEHHHHHHHHHHHHHHHCCEEECCCCEEEEECCCEEEEEEECCCCCC
NIMLNKMLGNQVKTLLKLAKTNNLAVIITNQVRETVNGFEASGGRLLEYWSKCIVRLEKL
CEEEHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHC
NGDRLAILEKHLHAGEERVKFRIVERGIEIID
CCCEEEEHHHHHHCCCHHHHHHEECCCCEECC
>Mature Secondary Structure
MLKEILLGNAEKGIITQIYGPPGVGKTNICIINSINAVNSGKVIYIDTEGGLSIERIKQI
CCCHHHCCCCCCCEEEEEECCCCCCCCCEEEEECCCCCCCCEEEEEECCCCCCHHHHHHH
ASNNYKIVLENMIIYNAFDFYEQDKIIQKELPLITNNASLIVVDNITSLYRLELSDEANK
HCCCCEEEEEEEEEEHHHHHHHHHHHHHHHCCEEECCCCEEEEECCCEEEEEEECCCCCC
NIMLNKMLGNQVKTLLKLAKTNNLAVIITNQVRETVNGFEASGGRLLEYWSKCIVRLEKL
CEEEHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHC
NGDRLAILEKHLHAGEERVKFRIVERGIEIID
CCCEEEEHHHHHHCCCHHHHHHEECCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087