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The map label for this gene is pcm

Identifier: 15668344

GI number: 15668344

Start: 175180

End: 175827

Strand: Reverse

Name: pcm

Synonym: MJ0172

Alternate gene names: 15668344

Gene position: 175827-175180 (Counterclockwise)

Preceding gene: 15668347

Following gene: 15668343

Centisome position: 10.56

GC content: 37.5

Gene sequence:

>648_bases
ATGGATTTAGAAGAACAAAAGAAAGCAGTAATTGAGAAATTAATTAGGGAGGGGTATATAAAAAGTAAAAGAGTAATTGA
TGCTCTATTAAAAGTTCCAAGGGAGGAATTTCTTCCAGAGCATTTAAAGGAATATGCGTATGTAGATACTCCATTAGAGA
TTGGTTATGGGCAGACTATTTCAGCCATTCATATGGTTGGAATGATGTGTGAGCTTTTAGATTTAAAGCCAGGAATGAAA
GTTTTAGAGATTGGGACTGGTTGTGGTTATCACGCGGCAGTAACTGCTGAGATTGTGGGGGAGGATGGTTTAGTTGTTAG
TATTGAGAGAATTCCAGAATTGGCTGAAAAAGCAGAGAGAACTTTAAGGAAATTGGGATACGATAACGTTATTGTAATAG
TGGGAGATGGAACTTTAGGGTATGAGCCATTAGCCCCTTATGATAGGATATATACAACTGCAGCAGGTCCAAAAATCCCA
GAACCATTAATAAGGCAATTAAAAGATGGGGGAAAGTTATTAATGCCTGTTGGTAGGTATCTACAAAGATTAGTTTTAGC
TGAAAAGAGAGGAGATGAGATAATAATAAAGGACTGTGGGCCAGTGGCATTTGTTCCTTTAGTTGGTAAAGAAGGATTCC
AAGGGTAA

Upstream 100 bases:

>100_bases
GCATAACACCAAATAATTATTTTGGTGGTAATTTCTTATAATTAACATTATATTAAACATTTATATAAGCTACCTTATAT
AGATTATATTTGGTGAAATT

Downstream 100 bases:

>100_bases
AATGATAATAAGATAAATTATTATCTCTTTTATTTTATTCTGTTTTATTTTTGAGTGGATAGTTAATGACATATTAACTA
AGATTAAAAATGAGAAACAA

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MDLEEQKKAVIEKLIREGYIKSKRVIDALLKVPREEFLPEHLKEYAYVDTPLEIGYGQTISAIHMVGMMCELLDLKPGMK
VLEIGTGCGYHAAVTAEIVGEDGLVVSIERIPELAEKAERTLRKLGYDNVIVIVGDGTLGYEPLAPYDRIYTTAAGPKIP
EPLIRQLKDGGKLLMPVGRYLQRLVLAEKRGDEIIIKDCGPVAFVPLVGKEGFQG

Sequences:

>Translated_215_residues
MDLEEQKKAVIEKLIREGYIKSKRVIDALLKVPREEFLPEHLKEYAYVDTPLEIGYGQTISAIHMVGMMCELLDLKPGMK
VLEIGTGCGYHAAVTAEIVGEDGLVVSIERIPELAEKAERTLRKLGYDNVIVIVGDGTLGYEPLAPYDRIYTTAAGPKIP
EPLIRQLKDGGKLLMPVGRYLQRLVLAEKRGDEIIIKDCGPVAFVPLVGKEGFQG
>Mature_215_residues
MDLEEQKKAVIEKLIREGYIKSKRVIDALLKVPREEFLPEHLKEYAYVDTPLEIGYGQTISAIHMVGMMCELLDLKPGMK
VLEIGTGCGYHAAVTAEIVGEDGLVVSIERIPELAEKAERTLRKLGYDNVIVIVGDGTLGYEPLAPYDRIYTTAAGPKIP
EPLIRQLKDGGKLLMPVGRYLQRLVLAEKRGDEIIIKDCGPVAFVPLVGKEGFQG

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=212, Percent_Identity=38.6792452830189, Blast_Score=118, Evalue=4e-27,
Organism=Homo sapiens, GI157388995, Length=215, Percent_Identity=26.5116279069767, Blast_Score=65, Evalue=3e-11,
Organism=Escherichia coli, GI1789100, Length=204, Percent_Identity=44.6078431372549, Blast_Score=157, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI71983477, Length=211, Percent_Identity=36.0189573459716, Blast_Score=117, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI193207222, Length=209, Percent_Identity=34.4497607655502, Blast_Score=107, Evalue=3e-24,
Organism=Drosophila melanogaster, GI17981723, Length=195, Percent_Identity=35.3846153846154, Blast_Score=105, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT_METJA (Q57636)

Other databases:

- EMBL:   L77117
- PIR:   E64321
- RefSeq:   NP_247140.1
- PDB:   2YXE
- PDBsum:   2YXE
- ProteinModelPortal:   Q57636
- SMR:   Q57636
- GeneID:   1451019
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0172
- NMPDR:   fig|243232.1.peg.177
- TIGR:   MJ0172
- HOGENOM:   HBG699907
- OMA:   LCPVRFV
- ProtClustDB:   PRK13942
- BioCyc:   MJAN243232:MJ_0172-MONOMER
- BRENDA:   2.1.1.77
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 23761; Mature: 23761

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 61-61

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLEEQKKAVIEKLIREGYIKSKRVIDALLKVPREEFLPEHLKEYAYVDTPLEIGYGQTI
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCHH
SAIHMVGMMCELLDLKPGMKVLEIGTGCGYHAAVTAEIVGEDGLVVSIERIPELAEKAER
HHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHEEEEEEECCCCCEEEHHHHHHHHHHHHH
TLRKLGYDNVIVIVGDGTLGYEPLAPYDRIYTTAAGPKIPEPLIRQLKDGGKLLMPVGRY
HHHHCCCCCEEEEEECCCCCCCCCCCHHHHEECCCCCCCCHHHHHHHHCCCCEEHHHHHH
LQRLVLAEKRGDEIIIKDCGPVAFVPLVGKEGFQG
HHHHHHHHCCCCEEEEECCCCEEEEEECCCCCCCC
>Mature Secondary Structure
MDLEEQKKAVIEKLIREGYIKSKRVIDALLKVPREEFLPEHLKEYAYVDTPLEIGYGQTI
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCHH
SAIHMVGMMCELLDLKPGMKVLEIGTGCGYHAAVTAEIVGEDGLVVSIERIPELAEKAER
HHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHEEEEEEECCCCCEEEHHHHHHHHHHHHH
TLRKLGYDNVIVIVGDGTLGYEPLAPYDRIYTTAAGPKIPEPLIRQLKDGGKLLMPVGRY
HHHHCCCCCEEEEEECCCCCCCCCCCHHHHEECCCCCCCCHHHHHHHHCCCCEEHHHHHH
LQRLVLAEKRGDEIIIKDCGPVAFVPLVGKEGFQG
HHHHHHHHCCCCEEEEECCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087