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Identifier: 15668343

GI number: 15668343

Start: 173302

End: 175023

Strand: Reverse

Name: lig

Synonym: MJ0171

Alternate gene names: 15668343

Gene position: 175023-173302 (Counterclockwise)

Preceding gene: 15668344

Following gene: 15668342

Centisome position: 10.51

GC content: 32.46

Gene sequence:

>1722_bases
ATGCTTTGGAGAGATGTTTGTGAAATATTTAATAAAATTGAAAAAACAACAAAAAGGTTGGAAAAGAGAGATTATTTTAT
AAAATTAATTGACATGGTTAAAGAGAAAGGGAAGCCAGAGGATTTAAAAAAGATTTGTTATATGGCTATAGGGAGGGTTT
ATCCCGAATACGATGAGAGAGAGTTAGGAATTGGAGAAAAACTTTTAATAAATGCTGTTACATCTATAGGAATTAAGAAA
GATGAATTGTTAGAGAAAATTAAAGAGACGGGAGATATTGGATTGGCAATAGAGCAATTAAAATCAAAGATTAAGCAAGC
ATCTTTATTTTTTCAGCCATTAACTGTAGATGAGGTTTATGAAACCTTAAAGAGGGTTGGGGAGATAGAGGGAGAAGGTT
CTCAAAAGAAAAAGTTGAGGTTAATAAGTAGTCTCTTTTTAAGAGCTTCACCAATAGAGTGCAGGTATTTGGCAAGGTTA
ATTTTGGAAGATATGAGGATAGGGATGAATGTTCCAACTATATTAGATGCTTTGTCAGTTTATTTCAATGTTCCAAAGGA
AAAACTTGAGAAGATATATGCTATAACCAATGATATTGGGCTTTTAGCTGAGAAATTATTAATGGGAGATTTAGAAAGTG
AGGAGCTAAAATTAAAATTATTTAGACCAATAAAACCAATGTTGGCTCAATTAACTCCTTCAATTGAAGAGGCATTATTG
GAGATGGGCAGAGCTCAATTTGAAACAAAGTATGATGGAGCAAGAGTTCAAATACATAAGGATGGAAATAAAGTTAAGAT
ATATAGCAGGAGATTGGAGGATGTTACAAATGCCCTTCCAGAGATTGTTGAGGCGGTAAAAAATATTAATGTAGATAAAT
TAATTGTTGAAGGGGAGTGTGTAGCTATAGATAAACAAACAGGAAAGCCAAGACCTTTCCAAGATATACTTAGAAGATTT
AGGAGAAAGTATGATATTGGAAAGATGATGAAGGAAATAAATTTGAGAGTTTATTTGTTTGATATTCTTTATAAAGATGG
AGTATCATTTATAGATGAGGAATTTGAAAAGAGAAGAAAAGTTTTAGAGGAAATTGTTGGTTATGAGAATGATTGGAGAA
CTGAAAGAAAGAGGATAGAGAAAGAGCTTAAATCAGATAAAATAATTGATATATCCTATAAATTAGTCACAAACGATGCA
AAAGAGGCAAGAGAATTTTATAACTGGTCTCTATCTATTGGGCATGAGGGAGTTATGATTAAAAATTTAAAGGCTCCTTA
TACCCCAGGAAGTAGAGTTAGAACAATGTATAAATTTAAACCAACTCTTGAGAGTTTAGATGTCGTAATTACAAAGGCAA
AGAGAGGGATGGGGAAGAGAAAGGATTGGTATGGTTCATTTGAAATATGTGTTAGAGATGAGGAAGGGAACCTCTACCCT
ATTGGACATGTAGGGACTGGACTAACTGAGGCAGATTTAGAGTTTTTGAAAGAAGAGATTGATAAAATTATTATTAGAGA
TTTAGGTGAAGAGGTTGAAGTAGAACCAAAGATAGTTATTGAAGTTGCTTATGAAGAAATTCAAAAATCTGATAAATATC
CTTGTGGCTATGCTTTGAGATTCCCAAGGGTTGTAAGATTTAGATTTGATAAGGGAGTTAATGAGATAAACACTATAGAG
GATGTTGAAAGGATATATGAAATCCAAAGAGGAAGGAAATAA

Upstream 100 bases:

>100_bases
GGATAGTTAATGACATATTAACTAAGATTAAAAATGAGAAACAATTTTAATTAGAGCCAAGATAATAAAATTTCATAATA
TATATTTTTATGGTGAAATA

Downstream 100 bases:

>100_bases
TCTTTAATATAAAAATCTTTGATAAATAATTAAATTTTTCAATTTTATTTTTATAGTGGTAATTTAAAGAAGAGGTGATG
TGAATATGGAAATTTTTGGG

Product: DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [ATP]

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MLWRDVCEIFNKIEKTTKRLEKRDYFIKLIDMVKEKGKPEDLKKICYMAIGRVYPEYDERELGIGEKLLINAVTSIGIKK
DELLEKIKETGDIGLAIEQLKSKIKQASLFFQPLTVDEVYETLKRVGEIEGEGSQKKKLRLISSLFLRASPIECRYLARL
ILEDMRIGMNVPTILDALSVYFNVPKEKLEKIYAITNDIGLLAEKLLMGDLESEELKLKLFRPIKPMLAQLTPSIEEALL
EMGRAQFETKYDGARVQIHKDGNKVKIYSRRLEDVTNALPEIVEAVKNINVDKLIVEGECVAIDKQTGKPRPFQDILRRF
RRKYDIGKMMKEINLRVYLFDILYKDGVSFIDEEFEKRRKVLEEIVGYENDWRTERKRIEKELKSDKIIDISYKLVTNDA
KEAREFYNWSLSIGHEGVMIKNLKAPYTPGSRVRTMYKFKPTLESLDVVITKAKRGMGKRKDWYGSFEICVRDEEGNLYP
IGHVGTGLTEADLEFLKEEIDKIIIRDLGEEVEVEPKIVIEVAYEEIQKSDKYPCGYALRFPRVVRFRFDKGVNEINTIE
DVERIYEIQRGRK

Sequences:

>Translated_573_residues
MLWRDVCEIFNKIEKTTKRLEKRDYFIKLIDMVKEKGKPEDLKKICYMAIGRVYPEYDERELGIGEKLLINAVTSIGIKK
DELLEKIKETGDIGLAIEQLKSKIKQASLFFQPLTVDEVYETLKRVGEIEGEGSQKKKLRLISSLFLRASPIECRYLARL
ILEDMRIGMNVPTILDALSVYFNVPKEKLEKIYAITNDIGLLAEKLLMGDLESEELKLKLFRPIKPMLAQLTPSIEEALL
EMGRAQFETKYDGARVQIHKDGNKVKIYSRRLEDVTNALPEIVEAVKNINVDKLIVEGECVAIDKQTGKPRPFQDILRRF
RRKYDIGKMMKEINLRVYLFDILYKDGVSFIDEEFEKRRKVLEEIVGYENDWRTERKRIEKELKSDKIIDISYKLVTNDA
KEAREFYNWSLSIGHEGVMIKNLKAPYTPGSRVRTMYKFKPTLESLDVVITKAKRGMGKRKDWYGSFEICVRDEEGNLYP
IGHVGTGLTEADLEFLKEEIDKIIIRDLGEEVEVEPKIVIEVAYEEIQKSDKYPCGYALRFPRVVRFRFDKGVNEINTIE
DVERIYEIQRGRK
>Mature_573_residues
MLWRDVCEIFNKIEKTTKRLEKRDYFIKLIDMVKEKGKPEDLKKICYMAIGRVYPEYDERELGIGEKLLINAVTSIGIKK
DELLEKIKETGDIGLAIEQLKSKIKQASLFFQPLTVDEVYETLKRVGEIEGEGSQKKKLRLISSLFLRASPIECRYLARL
ILEDMRIGMNVPTILDALSVYFNVPKEKLEKIYAITNDIGLLAEKLLMGDLESEELKLKLFRPIKPMLAQLTPSIEEALL
EMGRAQFETKYDGARVQIHKDGNKVKIYSRRLEDVTNALPEIVEAVKNINVDKLIVEGECVAIDKQTGKPRPFQDILRRF
RRKYDIGKMMKEINLRVYLFDILYKDGVSFIDEEFEKRRKVLEEIVGYENDWRTERKRIEKELKSDKIIDISYKLVTNDA
KEAREFYNWSLSIGHEGVMIKNLKAPYTPGSRVRTMYKFKPTLESLDVVITKAKRGMGKRKDWYGSFEICVRDEEGNLYP
IGHVGTGLTEADLEFLKEEIDKIIIRDLGEEVEVEPKIVIEVAYEEIQKSDKYPCGYALRFPRVVRFRFDKGVNEINTIE
DVERIYEIQRGRK

Specific function: DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair

COG id: COG1793

COG function: function code L; ATP-dependent DNA ligase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family

Homologues:

Organism=Homo sapiens, GI4557719, Length=629, Percent_Identity=28.6168521462639, Blast_Score=202, Evalue=1e-51,
Organism=Homo sapiens, GI46255052, Length=624, Percent_Identity=24.3589743589744, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI23199993, Length=624, Percent_Identity=24.3589743589744, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI148539894, Length=624, Percent_Identity=24.3589743589744, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI73747829, Length=528, Percent_Identity=25.3787878787879, Blast_Score=128, Evalue=2e-29,
Organism=Homo sapiens, GI73747844, Length=528, Percent_Identity=25.3787878787879, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71985269, Length=602, Percent_Identity=30.0664451827243, Blast_Score=202, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI71985278, Length=167, Percent_Identity=31.1377245508982, Blast_Score=91, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320038, Length=629, Percent_Identity=29.093799682035, Blast_Score=199, Evalue=9e-52,
Organism=Drosophila melanogaster, GI24762424, Length=635, Percent_Identity=27.0866141732283, Blast_Score=191, Evalue=1e-48,
Organism=Drosophila melanogaster, GI85815841, Length=569, Percent_Identity=24.9560632688928, Blast_Score=120, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24641829, Length=484, Percent_Identity=22.3140495867769, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLI_METJA (Q57635)

Other databases:

- EMBL:   L77117
- PIR:   D64321
- RefSeq:   NP_247139.1
- ProteinModelPortal:   Q57635
- SMR:   Q57635
- GeneID:   1451018
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0171
- NMPDR:   fig|243232.1.peg.176
- TIGR:   MJ0171
- HOGENOM:   HBG570821
- OMA:   GRPRPFQ
- ProtClustDB:   CLSK876100
- BioCyc:   MJAN243232:MJ_0171-MONOMER
- BRENDA:   6.5.1.1
- HAMAP:   MF_00407
- InterPro:   IPR022865
- InterPro:   IPR000977
- InterPro:   IPR012309
- InterPro:   IPR012310
- InterPro:   IPR016059
- InterPro:   IPR012308
- InterPro:   IPR012340
- InterPro:   IPR016027
- Gene3D:   G3DSA:2.40.50.140
- TIGRFAMs:   TIGR00574

Pfam domain/function: PF04679 DNA_ligase_A_C; PF01068 DNA_ligase_A_M; PF04675 DNA_ligase_A_N; SSF50249 Nucleic_acid_OB

EC number: =6.5.1.1

Molecular weight: Translated: 66791; Mature: 66791

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: PS00697 DNA_LIGASE_A1; PS00333 DNA_LIGASE_A2; PS50160 DNA_LIGASE_A3

Important sites: ACT_SITE 250-250 BINDING 248-248 BINDING 255-255 BINDING 270-270 BINDING 299-299 BINDING 340-340 BINDING 432-432 BINDING 438-438

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLWRDVCEIFNKIEKTTKRLEKRDYFIKLIDMVKEKGKPEDLKKICYMAIGRVYPEYDER
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
ELGIGEKLLINAVTSIGIKKDELLEKIKETGDIGLAIEQLKSKIKQASLFFQPLTVDEVY
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHH
ETLKRVGEIEGEGSQKKKLRLISSLFLRASPIECRYLARLILEDMRIGMNVPTILDALSV
HHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHH
YFNVPKEKLEKIYAITNDIGLLAEKLLMGDLESEELKLKLFRPIKPMLAQLTPSIEEALL
HHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHCCHHHHHHH
EMGRAQFETKYDGARVQIHKDGNKVKIYSRRLEDVTNALPEIVEAVKNINVDKLIVEGEC
HHHHHHHCCCCCCCEEEEEECCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEE
VAIDKQTGKPRPFQDILRRFRRKYDIGKMMKEINLRVYLFDILYKDGVSFIDEEFEKRRK
EEEECCCCCCCCHHHHHHHHHHHCCHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHHHHHH
VLEEIVGYENDWRTERKRIEKELKSDKIIDISYKLVTNDAKEAREFYNWSLSIGHEGVMI
HHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHCCEEEECCCCEEE
KNLKAPYTPGSRVRTMYKFKPTLESLDVVITKAKRGMGKRKDWYGSFEICVRDEEGNLYP
ECCCCCCCCCHHHEEHHEECCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCEEE
IGHVGTGLTEADLEFLKEEIDKIIIRDLGEEVEVEPKIVIEVAYEEIQKSDKYPCGYALR
ECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHEEHHHHHHHHCCCCCCCCEEEC
FPRVVRFRFDKGVNEINTIEDVERIYEIQRGRK
CCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLWRDVCEIFNKIEKTTKRLEKRDYFIKLIDMVKEKGKPEDLKKICYMAIGRVYPEYDER
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
ELGIGEKLLINAVTSIGIKKDELLEKIKETGDIGLAIEQLKSKIKQASLFFQPLTVDEVY
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHH
ETLKRVGEIEGEGSQKKKLRLISSLFLRASPIECRYLARLILEDMRIGMNVPTILDALSV
HHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHH
YFNVPKEKLEKIYAITNDIGLLAEKLLMGDLESEELKLKLFRPIKPMLAQLTPSIEEALL
HHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHCCHHHHHHH
EMGRAQFETKYDGARVQIHKDGNKVKIYSRRLEDVTNALPEIVEAVKNINVDKLIVEGEC
HHHHHHHCCCCCCCEEEEEECCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEE
VAIDKQTGKPRPFQDILRRFRRKYDIGKMMKEINLRVYLFDILYKDGVSFIDEEFEKRRK
EEEECCCCCCCCHHHHHHHHHHHCCHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHHHHHH
VLEEIVGYENDWRTERKRIEKELKSDKIIDISYKLVTNDAKEAREFYNWSLSIGHEGVMI
HHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHCCEEEECCCCEEE
KNLKAPYTPGSRVRTMYKFKPTLESLDVVITKAKRGMGKRKDWYGSFEICVRDEEGNLYP
ECCCCCCCCCHHHEEHHEECCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCEEE
IGHVGTGLTEADLEFLKEEIDKIIIRDLGEEVEVEPKIVIEVAYEEIQKSDKYPCGYALR
ECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHEEHHHHHHHHCCCCCCCCEEEC
FPRVVRFRFDKGVNEINTIEDVERIYEIQRGRK
CCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087