Click here to switch to the map view.

The map label for this gene is nrfB

Identifier: 30995421

GI number: 30995421

Start: 1131698

End: 1132345

Strand: Reverse

Name: nrfB

Synonym: HI1068

Alternate gene names: 30995421

Gene position: 1132345-1131698 (Counterclockwise)

Preceding gene: 161350017

Following gene: 16272998

Centisome position: 61.87

GC content: 37.5

Gene sequence:

>648_bases
ATGATTTTAACTTCTTTAATCAACAAATCTGCAAAAGCGTTAGTAATTGTGGCATTTGTAGCAGCGCCATTTTTGGCTCA
TGCGGATGATGCACAAAAACCTGCGGTTCATGTTACTTATGAACCGCAATTAGATAACCAACGCGATCCTAATCAATATT
GTGCTAAATGCCATAAATTCGACAAAATAGATAAAAACCAAACGCTAGATCAGTCTGGTGGCGAACTCCACTTTGGTAAA
TTTCACGGTGCACATTTAGATAAGAAAAATCCAAATAACGGTAAAGCTATTACTTGTGTAAGCTGTCACGGCAATATTTC
CGAAAATCATCGTCGCGGTGCTAAAGATGTTATGCGTTTTGAAGGAGATATTTTCGGTAATAAAAAACCGATGTATTCCG
TTCAAGAACAAAACCAAGTTTGTTTTGCTTGCCATCAACCTGACAAACTTCGTGAAAAATTATGGGCTCATGATGTACAC
GCAATGAAATTGCCTTGCGCAAGTTGCCATACTCTTCACCCTAAAGAAGATGCAATGAAGGGTATTCAACCAAAACAACG
CGTTAAACTTTGTGTAGATTGTCATGGCGAACAACAAAAACGTAAAGCTGAACAAGATAAACTAATCGAACAAAAGGATA
AACTATGA

Upstream 100 bases:

>100_bases
GTTGATCCACCAGCACAAAAATAAATGAAAAATGCACCGCACTTTTTAGTGCAAACTAGAAAGTGCGGTGATTTTCAAGG
TCAAATTTGAGGTAACCCAA

Downstream 100 bases:

>100_bases
CAGTCTGCTCACGCCGAAACTTTGTTTCTGGCATGGGGGCAGTGATCCTTATGACGGGGACATCTTTACCTGCTTTTGCA
AAAGAAAACAAGGCAGATAA

Product: cytochrome c nitrite reductase pentaheme subunit

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MILTSLINKSAKALVIVAFVAAPFLAHADDAQKPAVHVTYEPQLDNQRDPNQYCAKCHKFDKIDKNQTLDQSGGELHFGK
FHGAHLDKKNPNNGKAITCVSCHGNISENHRRGAKDVMRFEGDIFGNKKPMYSVQEQNQVCFACHQPDKLREKLWAHDVH
AMKLPCASCHTLHPKEDAMKGIQPKQRVKLCVDCHGEQQKRKAEQDKLIEQKDKL

Sequences:

>Translated_215_residues
MILTSLINKSAKALVIVAFVAAPFLAHADDAQKPAVHVTYEPQLDNQRDPNQYCAKCHKFDKIDKNQTLDQSGGELHFGK
FHGAHLDKKNPNNGKAITCVSCHGNISENHRRGAKDVMRFEGDIFGNKKPMYSVQEQNQVCFACHQPDKLREKLWAHDVH
AMKLPCASCHTLHPKEDAMKGIQPKQRVKLCVDCHGEQQKRKAEQDKLIEQKDKL
>Mature_215_residues
MILTSLINKSAKALVIVAFVAAPFLAHADDAQKPAVHVTYEPQLDNQRDPNQYCAKCHKFDKIDKNQTLDQSGGELHFGK
FHGAHLDKKNPNNGKAITCVSCHGNISENHRRGAKDVMRFEGDIFGNKKPMYSVQEQNQVCFACHQPDKLREKLWAHDVH
AMKLPCASCHTLHPKEDAMKGIQPKQRVKLCVDCHGEQQKRKAEQDKLIEQKDKL

Specific function: Plays a role in nitrite reduction

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082366, Length=198, Percent_Identity=38.8888888888889, Blast_Score=145, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NRFB_HAEIN (P45016)

Other databases:

- EMBL:   L42023
- PIR:   B64181
- RefSeq:   NP_439226.2
- ProteinModelPortal:   P45016
- SMR:   P45016
- GeneID:   950046
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1068
- NMPDR:   fig|71421.1.peg.1027
- TIGR:   HI_1068
- HOGENOM:   HBG678928
- OMA:   EQNQVCF
- ProtClustDB:   PRK11659
- BioCyc:   HINF71421:HI_1068-MONOMER
- GO:   GO:0006810
- InterPro:   IPR017564
- InterPro:   IPR011031
- TIGRFAMs:   TIGR03146

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 24312; Mature: 24312

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: BINDING 65-65 BINDING 68-68 BINDING 110-110 BINDING 113-113 BINDING 152-152 BINDING 155-155 BINDING 177-177 BINDING 180-180 BINDING 202-202 BINDING 205-205

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
4.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILTSLINKSAKALVIVAFVAAPFLAHADDAQKPAVHVTYEPQLDNQRDPNQYCAKCHKF
CCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHH
DKIDKNQTLDQSGGELHFGKFHGAHLDKKNPNNGKAITCVSCHGNISENHRRGAKDVMRF
HCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHCCHHHHHHH
EGDIFGNKKPMYSVQEQNQVCFACHQPDKLREKLWAHDVHAMKLPCASCHTLHPKEDAMK
CCCCCCCCCCCEEECCCCCEEEEECCCHHHHHHHHHHCHHHHCCCCCCCCCCCCHHHHHH
GIQPKQRVKLCVDCHGEQQKRKAEQDKLIEQKDKL
CCCHHHHHEEEEECCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MILTSLINKSAKALVIVAFVAAPFLAHADDAQKPAVHVTYEPQLDNQRDPNQYCAKCHKF
CCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHH
DKIDKNQTLDQSGGELHFGKFHGAHLDKKNPNNGKAITCVSCHGNISENHRRGAKDVMRF
HCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHCCHHHHHHH
EGDIFGNKKPMYSVQEQNQVCFACHQPDKLREKLWAHDVHAMKLPCASCHTLHPKEDAMK
CCCCCCCCCCCEEECCCCCEEEEECCCHHHHHHHHHHCHHHHCCCCCCCCCCCCHHHHHH
GIQPKQRVKLCVDCHGEQQKRKAEQDKLIEQKDKL
CCCHHHHHEEEEECCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800