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The map label for this gene is nrfA [H]

Identifier: 161350017

GI number: 161350017

Start: 1132422

End: 1134038

Strand: Reverse

Name: nrfA [H]

Synonym: HI1069

Alternate gene names: 161350017

Gene position: 1134038-1132422 (Counterclockwise)

Preceding gene: 161350021

Following gene: 30995421

Centisome position: 61.96

GC content: 39.76

Gene sequence:

>1617_bases
ATGAAAATATATCTACGTTTTGTATGGATATTAATAATAATTCTCAACTTTCTACTTAACCTTTTTATTACAACAAATGG
AGTAATTATCGTGAACGCATTCAAAAAAAGCCTTATTGTGGCAGCGTCTTTCGCGAGTTTAAGCTTATTTAACTCAGCCA
CTGCTGAGCTTGTATATAAGCCATTAGAACAACCTGTTGAACCCGCAAAACCTGATTTGAAAATTGAATCTGTAAATGAA
AAATTTGCAGAAAAATATCCAAATCAATATAACAGCTGGCGTTCTACAGCAAACGGCGATGGCGAAAACATTATTTATGC
TGATGAAGAAAATCCGCGCCTAATCGTACTTTGGGGTGGTTATGCATTTGCAAAAGAATATAACGCACCACGCGGTCACT
TTTATGCGGTAACAGATGTGCGTAATATTTTACGTACAGGTGCGCCAAAAACTGCAAATGATGGCCCTCAAGCTATGGCA
TGTTGGACATGTAAAGGTCCTGATGTTCCACGTTTAATCGCGGAATGGGGTGAGAAAGATTATTTCAATGCGAAATGGGC
AAAGGGAGGACCTGAAATCGTGAACTCAATTGGCTGTGCTGACTGTCACGACACAACCTCTAAAGATTTTGCAGAAGGCA
AACCTGCATTACGTATTGCTCGCCCACACGTATTACGCGCCCTTGATGCTTTAGAAAAAGCCACTGCTGAAAAAGATAAA
GCAGAAGGTCGCCCACACAACAATTTAAGCTTCAACACGGCGGCGCGTACTGAAAAACGTGCAGAAATTTGCGCAAACTG
CCATGTTGAATACTATTTTGCAGGGGACATCAAGCAAGTAACTTTCCCTTGGGATAATGGTCAAACCGTAGATGATATTG
AAAAATATTATGATGATATTGGTTTCACTGACTGGACTCACTCACTTTCTAAAGCACCAATGTTAAAAGCACAACACCCT
GACTTTGAAATTTGGTCTTTAGGTATGCACGGTAAAAATGGGGTAACTTGTGTAGATTGCCATATGCCAAAAGTACAAGG
TGCGGACGGTAAAGTTTACACCGATCACCAAATTCAAAATCCATTTGAGGCATTTGACAGCACTTGTGCAAATTGTCACG
ATCAAAGCAAAGAAAAATTACGCGATATTGTTACTTCACGTAAAAAAGAAGTGAAAGATGTAATGGGTCGTTTAGAAGAC
CAAGTTGTCAAAGCTCACTTTGAAGCAAAAGAGGCTTGGGACGCAGGTGCAACTAAAAAAGAAATGGAAGCAGCATTAAT
GGATATTCGTCATGCTCAATGGCGTTGGGATTACACTGCTGCAAGCCATGGTGGTCATATGCATGCACCTGAAGTAGTAC
TTCGTGTATTAGCTTCTGGCTTAGATAAAGTAGCAGACGCACGCACTAAACTTGCAGTAATCTTAACTAAACACGGTGTG
AAAACTCCAGTTCAAATCCCAGATATTTCAACTGCTGATAAAGCGTGGAAAGTAATGGGTATTGATATTGAAAAAGAACG
TAAAGCGAAAGAAGAATTCTTAAAAACTGTGGTACCACAATGGGAACAACAAGCTAGAGAAAAAGGCTTATTAGTTGATC
CACCAGCACAAAAATAA

Upstream 100 bases:

>100_bases
ATACCTCTTTTTGAGTATTTTAAAAGATAAGCTTGATCAAGCTCAAACTTTAGCATTCTGCGAGTAGAAAAACTTTTTTC
TTTTGGTATCTTACGCTTGA

Downstream 100 bases:

>100_bases
ATGAAAAATGCACCGCACTTTTTAGTGCAAACTAGAAAGTGCGGTGATTTTCAAGGTCAAATTTGAGGTAACCCAAATGA
TTTTAACTTCTTTAATCAAC

Product: cytochrome c552

Products: NA

Alternate protein names: Ammonia-forming cytochrome c nitrite reductase; Cytochrome c nitrite reductase [H]

Number of amino acids: Translated: 538; Mature: 538

Protein sequence:

>538_residues
MKIYLRFVWILIIILNFLLNLFITTNGVIIVNAFKKSLIVAASFASLSLFNSATAELVYKPLEQPVEPAKPDLKIESVNE
KFAEKYPNQYNSWRSTANGDGENIIYADEENPRLIVLWGGYAFAKEYNAPRGHFYAVTDVRNILRTGAPKTANDGPQAMA
CWTCKGPDVPRLIAEWGEKDYFNAKWAKGGPEIVNSIGCADCHDTTSKDFAEGKPALRIARPHVLRALDALEKATAEKDK
AEGRPHNNLSFNTAARTEKRAEICANCHVEYYFAGDIKQVTFPWDNGQTVDDIEKYYDDIGFTDWTHSLSKAPMLKAQHP
DFEIWSLGMHGKNGVTCVDCHMPKVQGADGKVYTDHQIQNPFEAFDSTCANCHDQSKEKLRDIVTSRKKEVKDVMGRLED
QVVKAHFEAKEAWDAGATKKEMEAALMDIRHAQWRWDYTAASHGGHMHAPEVVLRVLASGLDKVADARTKLAVILTKHGV
KTPVQIPDISTADKAWKVMGIDIEKERKAKEEFLKTVVPQWEQQAREKGLLVDPPAQK

Sequences:

>Translated_538_residues
MKIYLRFVWILIIILNFLLNLFITTNGVIIVNAFKKSLIVAASFASLSLFNSATAELVYKPLEQPVEPAKPDLKIESVNE
KFAEKYPNQYNSWRSTANGDGENIIYADEENPRLIVLWGGYAFAKEYNAPRGHFYAVTDVRNILRTGAPKTANDGPQAMA
CWTCKGPDVPRLIAEWGEKDYFNAKWAKGGPEIVNSIGCADCHDTTSKDFAEGKPALRIARPHVLRALDALEKATAEKDK
AEGRPHNNLSFNTAARTEKRAEICANCHVEYYFAGDIKQVTFPWDNGQTVDDIEKYYDDIGFTDWTHSLSKAPMLKAQHP
DFEIWSLGMHGKNGVTCVDCHMPKVQGADGKVYTDHQIQNPFEAFDSTCANCHDQSKEKLRDIVTSRKKEVKDVMGRLED
QVVKAHFEAKEAWDAGATKKEMEAALMDIRHAQWRWDYTAASHGGHMHAPEVVLRVLASGLDKVADARTKLAVILTKHGV
KTPVQIPDISTADKAWKVMGIDIEKERKAKEEFLKTVVPQWEQQAREKGLLVDPPAQK
>Mature_538_residues
MKIYLRFVWILIIILNFLLNLFITTNGVIIVNAFKKSLIVAASFASLSLFNSATAELVYKPLEQPVEPAKPDLKIESVNE
KFAEKYPNQYNSWRSTANGDGENIIYADEENPRLIVLWGGYAFAKEYNAPRGHFYAVTDVRNILRTGAPKTANDGPQAMA
CWTCKGPDVPRLIAEWGEKDYFNAKWAKGGPEIVNSIGCADCHDTTSKDFAEGKPALRIARPHVLRALDALEKATAEKDK
AEGRPHNNLSFNTAARTEKRAEICANCHVEYYFAGDIKQVTFPWDNGQTVDDIEKYYDDIGFTDWTHSLSKAPMLKAQHP
DFEIWSLGMHGKNGVTCVDCHMPKVQGADGKVYTDHQIQNPFEAFDSTCANCHDQSKEKLRDIVTSRKKEVKDVMGRLED
QVVKAHFEAKEAWDAGATKKEMEAALMDIRHAQWRWDYTAASHGGHMHAPEVVLRVLASGLDKVADARTKLAVILTKHGV
KTPVQIPDISTADKAWKVMGIDIEKERKAKEEFLKTVVPQWEQQAREKGLLVDPPAQK

Specific function: Plays a role in nitrite reduction [H]

COG id: COG3303

COG function: function code P; Formate-dependent nitrite reductase, periplasmic cytochrome c552 subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome c-552 family [H]

Homologues:

Organism=Escherichia coli, GI1790506, Length=469, Percent_Identity=55.4371002132196, Blast_Score=564, Evalue=1e-162,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003321
- InterPro:   IPR017570
- InterPro:   IPR011031 [H]

Pfam domain/function: PF02335 Cytochrom_C552 [H]

EC number: =1.7.2.2 [H]

Molecular weight: Translated: 60400; Mature: 60400

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIYLRFVWILIIILNFLLNLFITTNGVIIVNAFKKSLIVAASFASLSLFNSATAELVYK
CEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
PLEQPVEPAKPDLKIESVNEKFAEKYPNQYNSWRSTANGDGENIIYADEENPRLIVLWGG
HHHCCCCCCCCCCEEEHHHHHHHHHCCCHHHHHCCCCCCCCCEEEEECCCCCEEEEEECC
YAFAKEYNAPRGHFYAVTDVRNILRTGAPKTANDGPQAMACWTCKGPDVPRLIAEWGEKD
CHHHHHCCCCCCCEEEHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCHHHHHHHCCCCC
YFNAKWAKGGPEIVNSIGCADCHDTTSKDFAEGKPALRIARPHVLRALDALEKATAEKDK
CCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHH
AEGRPHNNLSFNTAARTEKRAEICANCHVEYYFAGDIKQVTFPWDNGQTVDDIEKYYDDI
HCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCEEEEECCCCCCCHHHHHHHHHHC
GFTDWTHSLSKAPMLKAQHPDFEIWSLGMHGKNGVTCVDCHMPKVQGADGKVYTDHQIQN
CCCHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCEEEECCCCCCCCCCCCEEECHHHCC
PFEAFDSTCANCHDQSKEKLRDIVTSRKKEVKDVMGRLEDQVVKAHFEAKEAWDAGATKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
EMEAALMDIRHAQWRWDYTAASHGGHMHAPEVVLRVLASGLDKVADARTKLAVILTKHGV
HHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCC
KTPVQIPDISTADKAWKVMGIDIEKERKAKEEFLKTVVPQWEQQAREKGLLVDPPAQK
CCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKIYLRFVWILIIILNFLLNLFITTNGVIIVNAFKKSLIVAASFASLSLFNSATAELVYK
CEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
PLEQPVEPAKPDLKIESVNEKFAEKYPNQYNSWRSTANGDGENIIYADEENPRLIVLWGG
HHHCCCCCCCCCCEEEHHHHHHHHHCCCHHHHHCCCCCCCCCEEEEECCCCCEEEEEECC
YAFAKEYNAPRGHFYAVTDVRNILRTGAPKTANDGPQAMACWTCKGPDVPRLIAEWGEKD
CHHHHHCCCCCCCEEEHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCHHHHHHHCCCCC
YFNAKWAKGGPEIVNSIGCADCHDTTSKDFAEGKPALRIARPHVLRALDALEKATAEKDK
CCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHH
AEGRPHNNLSFNTAARTEKRAEICANCHVEYYFAGDIKQVTFPWDNGQTVDDIEKYYDDI
HCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCEEEEECCCCCCCHHHHHHHHHHC
GFTDWTHSLSKAPMLKAQHPDFEIWSLGMHGKNGVTCVDCHMPKVQGADGKVYTDHQIQN
CCCHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCEEEECCCCCCCCCCCCEEECHHHCC
PFEAFDSTCANCHDQSKEKLRDIVTSRKKEVKDVMGRLEDQVVKAHFEAKEAWDAGATKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
EMEAALMDIRHAQWRWDYTAASHGGHMHAPEVVLRVLASGLDKVADARTKLAVILTKHGV
HHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCC
KTPVQIPDISTADKAWKVMGIDIEKERKAKEEFLKTVVPQWEQQAREKGLLVDPPAQK
CCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA