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The map label for this gene is nrfC

Identifier: 16272998

GI number: 16272998

Start: 1131024

End: 1131701

Strand: Reverse

Name: nrfC

Synonym: HI1067

Alternate gene names: 16272998

Gene position: 1131701-1131024 (Counterclockwise)

Preceding gene: 30995421

Following gene: 16272997

Centisome position: 61.84

GC content: 41.3

Gene sequence:

>678_bases
ATGACAGTCTGCTCACGCCGAAACTTTGTTTCTGGCATGGGGGCAGTGATCCTTATGACGGGGACATCTTTACCTGCTTT
TGCAAAAGAAAACAAGGCAGATAAACCTAAACGTTACGCAATGATACATGATGAAAATGCGTGTATCGGTTGTACAGCCT
GTATGGACTCATGTCGTGATGTAAACCAAGTGCCAGAAGGCGTTTCTCGCTTAGAGATTCTACGTAGTGAACCTTATGGG
GAATTTCCAAATCAAGAATACGAGTTCTTTCGTCAATCTTGCCAACATTGTACAAATGCACCTTGTGTAGCAGTTTGTCC
AACTGGTGCATCTTTTATTGATAAAGAGACTGGCATTGTAGATGTACATAAAGATTTATGCATAGGCTGCCAATACTGTA
TAGCAGTTTGCCCATATCGTGTACGTTTTATTCATCCAATACATCGTACCGCAGATAAATGTAACTTCTGCCGCGATACA
AATTTAGCCAATGGTAAACAACCTGCTTGTGTAGAGGCTTGCCCAACCAAAGCATTAACCTTTGGGGATATGAATGATCC
AACAAGTGCAGTTTCACGTAAAGTGAAAGAAAAACCGGTTTATCGCACTAAAGTGGAATTAGGTACACAACCAAACTTAT
ATCACATTCCATTCCAACATGGGGAGCCTAGAAGATGA

Upstream 100 bases:

>100_bases
TTCAACCAAAACAACGCGTTAAACTTTGTGTAGATTGTCATGGCGAACAACAAAAACGTAAAGCTGAACAAGATAAACTA
ATCGAACAAAAGGATAAACT

Downstream 100 bases:

>100_bases
CGTTAGATTATCCAGTTCCATTTCATACGCCTAATCTAGTGTGGGATTATACCATTGCGATCTACTTGTTTTTATTGGGG
ATCTCATCTGGTGCGGTACA

Product: nitrite reductase Fe-S protein

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MTVCSRRNFVSGMGAVILMTGTSLPAFAKENKADKPKRYAMIHDENACIGCTACMDSCRDVNQVPEGVSRLEILRSEPYG
EFPNQEYEFFRQSCQHCTNAPCVAVCPTGASFIDKETGIVDVHKDLCIGCQYCIAVCPYRVRFIHPIHRTADKCNFCRDT
NLANGKQPACVEACPTKALTFGDMNDPTSAVSRKVKEKPVYRTKVELGTQPNLYHIPFQHGEPRR

Sequences:

>Translated_225_residues
MTVCSRRNFVSGMGAVILMTGTSLPAFAKENKADKPKRYAMIHDENACIGCTACMDSCRDVNQVPEGVSRLEILRSEPYG
EFPNQEYEFFRQSCQHCTNAPCVAVCPTGASFIDKETGIVDVHKDLCIGCQYCIAVCPYRVRFIHPIHRTADKCNFCRDT
NLANGKQPACVEACPTKALTFGDMNDPTSAVSRKVKEKPVYRTKVELGTQPNLYHIPFQHGEPRR
>Mature_224_residues
TVCSRRNFVSGMGAVILMTGTSLPAFAKENKADKPKRYAMIHDENACIGCTACMDSCRDVNQVPEGVSRLEILRSEPYGE
FPNQEYEFFRQSCQHCTNAPCVAVCPTGASFIDKETGIVDVHKDLCIGCQYCIAVCPYRVRFIHPIHRTADKCNFCRDTN
LANGKQPACVEACPTKALTFGDMNDPTSAVSRKVKEKPVYRTKVELGTQPNLYHIPFQHGEPRR

Specific function: Probably involved in the transfer of electrons from the quinone pool to the type-c cytochromes

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Escherichia coli, GI2367345, Length=218, Percent_Identity=64.2201834862385, Blast_Score=308, Evalue=2e-85,
Organism=Escherichia coli, GI226510944, Length=216, Percent_Identity=46.2962962962963, Blast_Score=196, Evalue=1e-51,
Organism=Escherichia coli, GI1789370, Length=222, Percent_Identity=31.5315315315315, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1790326, Length=179, Percent_Identity=30.1675977653631, Blast_Score=97, Evalue=6e-22,
Organism=Escherichia coli, GI1787749, Length=215, Percent_Identity=27.906976744186, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787122, Length=193, Percent_Identity=31.6062176165803, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1787872, Length=193, Percent_Identity=31.6062176165803, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1787740, Length=145, Percent_Identity=29.6551724137931, Blast_Score=77, Evalue=6e-16,
Organism=Escherichia coli, GI1787478, Length=137, Percent_Identity=30.6569343065693, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789067, Length=160, Percent_Identity=31.25, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1789079, Length=188, Percent_Identity=28.1914893617021, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI87082180, Length=166, Percent_Identity=30.7228915662651, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI2367245, Length=173, Percent_Identity=30.635838150289, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788811, Length=168, Percent_Identity=30.952380952381, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI87082179, Length=92, Percent_Identity=36.9565217391304, Blast_Score=64, Evalue=6e-12,
Organism=Escherichia coli, GI1787963, Length=160, Percent_Identity=26.25, Blast_Score=61, Evalue=5e-11,
Organism=Escherichia coli, GI87082114, Length=190, Percent_Identity=26.8421052631579, Blast_Score=60, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NRFC_HAEIN (P45015)

Other databases:

- EMBL:   L42023
- PIR:   A64181
- RefSeq:   NP_439225.1
- ProteinModelPortal:   P45015
- SMR:   P45015
- GeneID:   950045
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1067
- TIGR:   HI_1067
- HOGENOM:   HBG726839
- OMA:   AYSMEDG
- ProtClustDB:   CLSK869937
- BioCyc:   HINF71421:HI_1067-MONOMER
- GO:   GO:0006810
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR017567
- InterPro:   IPR006311
- TIGRFAMs:   TIGR03149
- TIGRFAMs:   TIGR01409

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 25105; Mature: 24974

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2; PS51318 TAT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
10.2 %Cys+Met (Translated Protein)
7.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
9.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVCSRRNFVSGMGAVILMTGTSLPAFAKENKADKPKRYAMIHDENACIGCTACMDSCRD
CCCCCCCCHHHCCCEEEEEECCCCCCHHHCCCCCCCCEEEEEECCCCEEEHHHHHHHHHH
VNQVPEGVSRLEILRSEPYGEFPNQEYEFFRQSCQHCTNAPCVAVCPTGASFIDKETGIV
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHCCCCCEE
DVHKDLCIGCQYCIAVCPYRVRFIHPIHRTADKCNFCRDTNLANGKQPACVEACPTKALT
ECHHHHHHHHHHHHHHCCHHHEEHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCEEE
FGDMNDPTSAVSRKVKEKPVYRTKVELGTQPNLYHIPFQHGEPRR
CCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCEEECCCCCCCCCC
>Mature Secondary Structure 
TVCSRRNFVSGMGAVILMTGTSLPAFAKENKADKPKRYAMIHDENACIGCTACMDSCRD
CCCCCCCHHHCCCEEEEEECCCCCCHHHCCCCCCCCEEEEEECCCCEEEHHHHHHHHHH
VNQVPEGVSRLEILRSEPYGEFPNQEYEFFRQSCQHCTNAPCVAVCPTGASFIDKETGIV
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHCCCCCEE
DVHKDLCIGCQYCIAVCPYRVRFIHPIHRTADKCNFCRDTNLANGKQPACVEACPTKALT
ECHHHHHHHHHHHHHHCCHHHEEHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCEEE
FGDMNDPTSAVSRKVKEKPVYRTKVELGTQPNLYHIPFQHGEPRR
CCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800