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The map label for this gene is sppA

Identifier: 16273441

GI number: 16273441

Start: 1611994

End: 1613841

Strand: Reverse

Name: sppA

Synonym: HI1541

Alternate gene names: 16273441

Gene position: 1613841-1611994 (Counterclockwise)

Preceding gene: 16273443

Following gene: 16273433

Centisome position: 88.18

GC content: 37.88

Gene sequence:

>1848_bases
ATGTTTCAAGTATTAAAATTTTGTTGGAAAGTGCTGTGTTTCATTCGTGATCTTGTGATGAATGTCGTGTTTTTAGGCTT
TGTACTATTGCTAGTGGCGATTATTAGCTTTTCAAGTGGCGGGAAAAAATCTACTGCATTAACGAGCGAAGGCGCATTAT
TGCTCAATTTAGATGGTTATTTAGCGGACAACCGAGATGAAACACTTCGTTGGCAAGATGCGTTAAGCGAATTGAATGGC
GAGCACGTCCCTAGAAAAATTTCGACTTTTGATGTGGTATTTGCCATTCAACAAGCGGAAGATGATCCTAAAATTAAAGG
ATTAGTGTTAGATCTTAATTATTTTGAAGGGGCAGATTTGCCCGCATTAGATTTTATTGGTGGTGCTATCAGTCACTTTA
AAGATGCAGGGAAACCAGTGATCGCCTATGCAGATAATTATTCACAAGGACAATATTATTTAGCGAGCTTTGCTGATGAA
ATTTATTTAAATTCAATTGGAAGCGTGGATATTCACGGTTTATCGCAAGAGAATTTGTATTTTAAAGAGATGCTTGACAA
ATTAGCCGTTACACCACATATTTTCCGCGTAGGGACTTATAAATCAGCAGTTGAACCATTTTTACGCAACGATATGTCGG
CTGAGGCAAAAGCAAATATGCAACGTTGGTTAGGCGAAATGTGGAATAATTATGTTTTATCCGTCAGTGAAAATCGTAAT
ATTAAGAAAGATCGCATTTTGCCGAATGCGAAACAGTATCTTGCAGAACTTAAAGCGTTAAAAGGCAATAGTACGGCATA
CGCACAACAACGTGGATTAGTAACTGATGTTGTAACTCGTTTGGATTTAGATAAAAAACTATCCGCACTTTTTGGCAAAG
GAAGTGATGGAAAAGCGAACCTTATCGAATTTGATGATTATTTGACTCAACTGCCTGATCGTTTAGAACATTACAACGTA
CCGAATAAAATTGCGGTTGTGAATGTTGAAGGAACGATTATTGATGGAGAAAGTGATGAAGAAAATGCTGGTGGCGACAC
CATTGCACGAATTCTTCGTAAAGCCCACGATGACAATTCTGTGAAAGCCGTGATTCTACGCGTTAATTCACCTGGCGGAA
GTGCTTTTGCTTCTGAAATTATCCGACAAGAAACTGAAAACTTACAGAAAATTGGAAAGCCTGTCATCGTATCTATGGGT
GCAATGGCAGCATCAGGTGGTTATTGGATTTCATCCACTGCAGATTATATTATTGCCGATTCAAATACAATTACGGGTTC
TATCGGTATTTTCACGATGTTCCCGACTTTCGAAAATAGTATTAAGAAAATAGGCGTGCATGCAGATGGTGTTTCAACCA
CTGAATTAGCTAATACATCTGCGTTTTCTCCATTAGCAAAACCCGTGCAAGATATTTATCAAACAGAAATTGAGCACGGT
TATGACAGATTTTTAGAAATTGTCAGCAAAGGTCGCCAGCTTTCTAAAACACAAGTAGATAAACTGGCTCAAGGGCAGGT
TTGGTTGGGCAGTGATGCATTCCAAAATGGTTTAGTGGATGAGATAGGTTCTTTTAATGAAGCGGTCAATAAAGCAGAGC
AATTAGTGAATCAACGCCAAGATACTGCCGTGCAAGATTTTAGCGTGGAATGGTTTACAGATGATAACGTTAGTTTAATT
AGCACCTTATTGAGCGATACTAAAAAAGGGGCGCAAGAGCAGTTAGTTAAATGGCTTGGTTTGCCTGCGCCAATTCAAAA
ATTACAAAAAGAATTGAATATATTAACTAAATTTAATGATCCTAAAGGTCAGTATTTGTATTGTTTGAATTGTGGAAAAG
TGAAGTAA

Upstream 100 bases:

>100_bases
CATTTTATTATCCCTTATAAGTAAAAGTGCGGTGGATTATAGCATAGTTTTTGACAAGGCTTTGTGATACGCTAGCACAA
ATTTTTTAAAGGTAATTTGT

Downstream 100 bases:

>100_bases
TAATTGAAAAAGGGCAGAATTAAATCTGCCCTTTCGCTTACAAAATATACGCTTCTTGAATATGATGTGGCTTTTGGTCT
TCTTCTGGTGGAAGCTTCAT

Product: protease IV

Products: NA

Alternate protein names: Endopeptidase IV; Protease IV; Signal peptide peptidase

Number of amino acids: Translated: 615; Mature: 615

Protein sequence:

>615_residues
MFQVLKFCWKVLCFIRDLVMNVVFLGFVLLLVAIISFSSGGKKSTALTSEGALLLNLDGYLADNRDETLRWQDALSELNG
EHVPRKISTFDVVFAIQQAEDDPKIKGLVLDLNYFEGADLPALDFIGGAISHFKDAGKPVIAYADNYSQGQYYLASFADE
IYLNSIGSVDIHGLSQENLYFKEMLDKLAVTPHIFRVGTYKSAVEPFLRNDMSAEAKANMQRWLGEMWNNYVLSVSENRN
IKKDRILPNAKQYLAELKALKGNSTAYAQQRGLVTDVVTRLDLDKKLSALFGKGSDGKANLIEFDDYLTQLPDRLEHYNV
PNKIAVVNVEGTIIDGESDEENAGGDTIARILRKAHDDNSVKAVILRVNSPGGSAFASEIIRQETENLQKIGKPVIVSMG
AMAASGGYWISSTADYIIADSNTITGSIGIFTMFPTFENSIKKIGVHADGVSTTELANTSAFSPLAKPVQDIYQTEIEHG
YDRFLEIVSKGRQLSKTQVDKLAQGQVWLGSDAFQNGLVDEIGSFNEAVNKAEQLVNQRQDTAVQDFSVEWFTDDNVSLI
STLLSDTKKGAQEQLVKWLGLPAPIQKLQKELNILTKFNDPKGQYLYCLNCGKVK

Sequences:

>Translated_615_residues
MFQVLKFCWKVLCFIRDLVMNVVFLGFVLLLVAIISFSSGGKKSTALTSEGALLLNLDGYLADNRDETLRWQDALSELNG
EHVPRKISTFDVVFAIQQAEDDPKIKGLVLDLNYFEGADLPALDFIGGAISHFKDAGKPVIAYADNYSQGQYYLASFADE
IYLNSIGSVDIHGLSQENLYFKEMLDKLAVTPHIFRVGTYKSAVEPFLRNDMSAEAKANMQRWLGEMWNNYVLSVSENRN
IKKDRILPNAKQYLAELKALKGNSTAYAQQRGLVTDVVTRLDLDKKLSALFGKGSDGKANLIEFDDYLTQLPDRLEHYNV
PNKIAVVNVEGTIIDGESDEENAGGDTIARILRKAHDDNSVKAVILRVNSPGGSAFASEIIRQETENLQKIGKPVIVSMG
AMAASGGYWISSTADYIIADSNTITGSIGIFTMFPTFENSIKKIGVHADGVSTTELANTSAFSPLAKPVQDIYQTEIEHG
YDRFLEIVSKGRQLSKTQVDKLAQGQVWLGSDAFQNGLVDEIGSFNEAVNKAEQLVNQRQDTAVQDFSVEWFTDDNVSLI
STLLSDTKKGAQEQLVKWLGLPAPIQKLQKELNILTKFNDPKGQYLYCLNCGKVK
>Mature_615_residues
MFQVLKFCWKVLCFIRDLVMNVVFLGFVLLLVAIISFSSGGKKSTALTSEGALLLNLDGYLADNRDETLRWQDALSELNG
EHVPRKISTFDVVFAIQQAEDDPKIKGLVLDLNYFEGADLPALDFIGGAISHFKDAGKPVIAYADNYSQGQYYLASFADE
IYLNSIGSVDIHGLSQENLYFKEMLDKLAVTPHIFRVGTYKSAVEPFLRNDMSAEAKANMQRWLGEMWNNYVLSVSENRN
IKKDRILPNAKQYLAELKALKGNSTAYAQQRGLVTDVVTRLDLDKKLSALFGKGSDGKANLIEFDDYLTQLPDRLEHYNV
PNKIAVVNVEGTIIDGESDEENAGGDTIARILRKAHDDNSVKAVILRVNSPGGSAFASEIIRQETENLQKIGKPVIVSMG
AMAASGGYWISSTADYIIADSNTITGSIGIFTMFPTFENSIKKIGVHADGVSTTELANTSAFSPLAKPVQDIYQTEIEHG
YDRFLEIVSKGRQLSKTQVDKLAQGQVWLGSDAFQNGLVDEIGSFNEAVNKAEQLVNQRQDTAVQDFSVEWFTDDNVSLI
STLLSDTKKGAQEQLVKWLGLPAPIQKLQKELNILTKFNDPKGQYLYCLNCGKVK

Specific function: Digestion of the cleaved signal peptides. This activity is necessary to maintain proper secretion of mature proteins across the membrane

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family

Homologues:

Organism=Escherichia coli, GI1788064, Length=629, Percent_Identity=43.0842607313196, Blast_Score=499, Evalue=1e-142,
Organism=Escherichia coli, GI1787527, Length=170, Percent_Identity=27.6470588235294, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPPA_HAEIN (P45243)

Other databases:

- EMBL:   L42023
- PIR:   F64128
- RefSeq:   NP_439690.1
- ProteinModelPortal:   P45243
- SMR:   P45243
- MEROPS:   S49.001
- GeneID:   950404
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1541
- NMPDR:   fig|71421.1.peg.1468
- TIGR:   HI_1541
- HOGENOM:   HBG686572
- OMA:   GGYWIAT
- ProtClustDB:   CLSK870195
- BioCyc:   HINF71421:HI_1541-MONOMER
- InterPro:   IPR004634
- InterPro:   IPR004635
- InterPro:   IPR002142
- PIRSF:   PIRSF001217
- TIGRFAMs:   TIGR00705
- TIGRFAMs:   TIGR00706

Pfam domain/function: PF01343 Peptidase_S49

EC number: 3.4.21.-

Molecular weight: Translated: 68058; Mature: 68058

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: ACT_SITE 201-201 ACT_SITE 405-405

Signals:

None

Transmembrane regions:

HASH(0xe9b2338)-;

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQVLKFCWKVLCFIRDLVMNVVFLGFVLLLVAIISFSSGGKKSTALTSEGALLLNLDGY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCEEEEEECCC
LADNRDETLRWQDALSELNGEHVPRKISTFDVVFAIQQAEDDPKIKGLVLDLNYFEGADL
CCCCCCHHHHHHHHHHHCCCCCCCCHHHHHEEEEEEECCCCCCCEEEEEEEEECCCCCCC
PALDFIGGAISHFKDAGKPVIAYADNYSQGQYYLASFADEIYLNSIGSVDIHGLSQENLY
CHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCHH
FKEMLDKLAVTPHIFRVGTYKSAVEPFLRNDMSAEAKANMQRWLGEMWNNYVLSVSENRN
HHHHHHHHCCCCHHEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEECCCCC
IKKDRILPNAKQYLAELKALKGNSTAYAQQRGLVTDVVTRLDLDKKLSALFGKGSDGKAN
CCHHHCCCCHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHCHHHHHHHHHCCCCCCCCC
LIEFDDYLTQLPDRLEHYNVPNKIAVVNVEGTIIDGESDEENAGGDTIARILRKAHDDNS
EEEHHHHHHHHHHHHHHCCCCCEEEEEEECEEEECCCCCCCCCCHHHHHHHHHHHCCCCC
VKAVILRVNSPGGSAFASEIIRQETENLQKIGKPVIVSMGAMAASGGYWISSTADYIIAD
EEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCHHCCCCEEEECCCCEEEEC
SNTITGSIGIFTMFPTFENSIKKIGVHADGVSTTELANTSAFSPLAKPVQDIYQTEIEHG
CCEEEECEEEEEECCHHHHHHHHCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHH
YDRFLEIVSKGRQLSKTQVDKLAQGQVWLGSDAFQNGLVDEIGSFNEAVNKAEQLVNQRQ
HHHHHHHHHCCCHHHHHHHHHHHCCCEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
DTAVQDFSVEWFTDDNVSLISTLLSDTKKGAQEQLVKWLGLPAPIQKLQKELNILTKFND
CCHHHHCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
PKGQYLYCLNCGKVK
CCCCEEEEECCCCCH
>Mature Secondary Structure
MFQVLKFCWKVLCFIRDLVMNVVFLGFVLLLVAIISFSSGGKKSTALTSEGALLLNLDGY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCEEEEEECCC
LADNRDETLRWQDALSELNGEHVPRKISTFDVVFAIQQAEDDPKIKGLVLDLNYFEGADL
CCCCCCHHHHHHHHHHHCCCCCCCCHHHHHEEEEEEECCCCCCCEEEEEEEEECCCCCCC
PALDFIGGAISHFKDAGKPVIAYADNYSQGQYYLASFADEIYLNSIGSVDIHGLSQENLY
CHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCHH
FKEMLDKLAVTPHIFRVGTYKSAVEPFLRNDMSAEAKANMQRWLGEMWNNYVLSVSENRN
HHHHHHHHCCCCHHEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEECCCCC
IKKDRILPNAKQYLAELKALKGNSTAYAQQRGLVTDVVTRLDLDKKLSALFGKGSDGKAN
CCHHHCCCCHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHCHHHHHHHHHCCCCCCCCC
LIEFDDYLTQLPDRLEHYNVPNKIAVVNVEGTIIDGESDEENAGGDTIARILRKAHDDNS
EEEHHHHHHHHHHHHHHCCCCCEEEEEEECEEEECCCCCCCCCCHHHHHHHHHHHCCCCC
VKAVILRVNSPGGSAFASEIIRQETENLQKIGKPVIVSMGAMAASGGYWISSTADYIIAD
EEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCHHCCCCEEEECCCCEEEEC
SNTITGSIGIFTMFPTFENSIKKIGVHADGVSTTELANTSAFSPLAKPVQDIYQTEIEHG
CCEEEECEEEEEECCHHHHHHHHCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHH
YDRFLEIVSKGRQLSKTQVDKLAQGQVWLGSDAFQNGLVDEIGSFNEAVNKAEQLVNQRQ
HHHHHHHHHCCCHHHHHHHHHHHCCCEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
DTAVQDFSVEWFTDDNVSLISTLLSDTKKGAQEQLVKWLGLPAPIQKLQKELNILTKFND
CCHHHHCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
PKGQYLYCLNCGKVK
CCCCEEEEECCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023