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The map label for this gene is phoR

Identifier: 16273289

GI number: 16273289

Start: 1474351

End: 1475628

Strand: Reverse

Name: phoR

Synonym: HI1378

Alternate gene names: 16273289

Gene position: 1475628-1474351 (Counterclockwise)

Preceding gene: 16273290

Following gene: 16273282

Centisome position: 80.63

GC content: 27.88

Gene sequence:

>1278_bases
ATGAAAAAAATATTGAATTTTATTGTTGAAATAAATTTGGCAATCATTATTTCCTTATTTACTTCTGATTTTATTCTTTG
GTTTGCAATAATACTTTTATTAATTTTAGCTTGGCATCATATTAATGAATATCGTTTACTCAAATATCTCAATTTAAAGC
AAGATAATAAATTCTCTTTATTACAATTAGGAACATTCTCTCAAACTGAAGCCTACCATCGTCATCAAATTTACAAAGAA
AAATGTGCAAGTTTACGTTTGTTATCTCAAATAAATAAAAATATTAAGTATTTACCAGATGCAATTATTATCTGTCAGCA
TAATGGTAATATTTCTTGGTGTAATTCAATTGCGCCACAGATGTTTGATTTTTGTTGGGATAAAAAAGTCCAGGAAAATA
TTTTTGATGTTATTTTTTATGAACAATTTAAACATTATTTTTTCAGTCCAAAAAAAAGACGACCGTTAGTACTATTAACT
TATAATCAACGTTATATTGAGGTGCAGTCGCACGCTTATAATTCTCATATGATTTTAGTTATCGCTCGAGATATTACAGA
TATGATTCATTTACTTAATTCACGCCAAAAATTCTTGAGTAATATTAATCACGAGCTACGTACTCCATTAACCGTATTAC
AAGGTTACTTAGAAATTCTTGCAGATAATAATATTCAGAACCCTCTACAAAAAAAGGCTATTTTMGCAATGCAAGAGCAA
AGCCAACGAATGGAACATCTATTACAACAATTTAATTTTTTAGCAAAAATAGAAACAACTTCAGATAAGGATTTTCGTAA
ATTTGATATGTCAGCCATGATAAATTCTTTAAGAAAAGATACAGATATATTAAATACTTATAATCATCACATTGAATTTA
TTATTCAACCTAATATCATTATTTTTGGAAATGAATCTCAATTAAGAAGTGCCGTTTCAAATTTAATTTATAATGCTATT
AAGCATTCTGGTAAACAGTGTCATATTCAAATTCAATGGGAAACTTGTGAACAGGGTATTAAATTTAACGTGATAGATAA
TGGGGTTGGCATATCACCACAGCATATTCCACATTTAACAGAACGTTTTTATAGAGTTGATGAATCTCGTAGCCATTTAA
CTGGTGGAAGTGGTTTAGGACTAGCTATTGTGAAGCATACTTTATTACAATATCATTCTCATTTAAATATTGAAAGTACT
GAAACGAAGGGTAGTAGTTTTTCGTTTATCATACCTAAACGCTTTGTCATATCAAAAAATAATAAAGAAATTCAATGA

Upstream 100 bases:

>100_bases
GCCGTTTACGCCGTAATTTAGCCCCTTTTCAATGTGAACATTACATCCAAACAGTTCGAGGATCTGGTTATCGATTTTCT
AGTTATTTACGAGATAAACA

Downstream 100 bases:

>100_bases
TAATACATGTTGCAAGTATTAGCTGACACGGTAGTAAATAACACATCAGAAAATCAAAAATGGTGGGCTAGTGGAGAGCT
TAGATACATAAAAAACACAG

Product: phosphate regulon sensor protein PhoR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MKKILNFIVEINLAIIISLFTSDFILWFAIILLLILAWHHINEYRLLKYLNLKQDNKFSLLQLGTFSQTEAYHRHQIYKE
KCASLRLLSQINKNIKYLPDAIIICQHNGNISWCNSIAPQMFDFCWDKKVQENIFDVIFYEQFKHYFFSPKKRRPLVLLT
YNQRYIEVQSHAYNSHMILVIARDITDMIHLLNSRQKFLSNINHELRTPLTVLQGYLEILADNNIQNPLQKKAIXAMQEQ
SQRMEHLLQQFNFLAKIETTSDKDFRKFDMSAMINSLRKDTDILNTYNHHIEFIIQPNIIIFGNESQLRSAVSNLIYNAI
KHSGKQCHIQIQWETCEQGIKFNVIDNGVGISPQHIPHLTERFYRVDESRSHLTGGSGLGLAIVKHTLLQYHSHLNIEST
ETKGSSFSFIIPKRFVISKNNKEIQ

Sequences:

>Translated_425_residues
MKKILNFIVEINLAIIISLFTSDFILWFAIILLLILAWHHINEYRLLKYLNLKQDNKFSLLQLGTFSQTEAYHRHQIYKE
KCASLRLLSQINKNIKYLPDAIIICQHNGNISWCNSIAPQMFDFCWDKKVQENIFDVIFYEQFKHYFFSPKKRRPLVLLT
YNQRYIEVQSHAYNSHMILVIARDITDMIHLLNSRQKFLSNINHELRTPLTVLQGYLEILADNNIQNPLQKKAIXAMQEQ
SQRMEHLLQQFNFLAKIETTSDKDFRKFDMSAMINSLRKDTDILNTYNHHIEFIIQPNIIIFGNESQLRSAVSNLIYNAI
KHSGKQCHIQIQWETCEQGIKFNVIDNGVGISPQHIPHLTERFYRVDESRSHLTGGSGLGLAIVKHTLLQYHSHLNIEST
ETKGSSFSFIIPKRFVISKNNKEIQ
>Mature_425_residues
MKKILNFIVEINLAIIISLFTSDFILWFAIILLLILAWHHINEYRLLKYLNLKQDNKFSLLQLGTFSQTEAYHRHQIYKE
KCASLRLLSQINKNIKYLPDAIIICQHNGNISWCNSIAPQMFDFCWDKKVQENIFDVIFYEQFKHYFFSPKKRRPLVLLT
YNQRYIEVQSHAYNSHMILVIARDITDMIHLLNSRQKFLSNINHELRTPLTVLQGYLEILADNNIQNPLQKKAIXAMQEQ
SQRMEHLLQQFNFLAKIETTSDKDFRKFDMSAMINSLRKDTDILNTYNHHIEFIIQPNIIIFGNESQLRSAVSNLIYNAI
KHSGKQCHIQIQWETCEQGIKFNVIDNGVGISPQHIPHLTERFYRVDESRSHLTGGSGLGLAIVKHTLLQYHSHLNIEST
ETKGSSFSFIIPKRFVISKNNKEIQ

Specific function: Member of the two-component regulatory system phoR/phoB involved in the phosphate regulon genes expression. PhoR may function as a membrane-associated protein kinase that phosphorylates phoB in response to environmental signals

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain

Homologues:

Organism=Escherichia coli, GI1786600, Length=414, Percent_Identity=38.6473429951691, Blast_Score=302, Evalue=3e-83,
Organism=Escherichia coli, GI1790346, Length=243, Percent_Identity=27.9835390946502, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786783, Length=232, Percent_Identity=29.3103448275862, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788393, Length=224, Percent_Identity=25, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1787894, Length=226, Percent_Identity=27.4336283185841, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI145693157, Length=226, Percent_Identity=26.1061946902655, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI48994928, Length=288, Percent_Identity=23.6111111111111, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789808, Length=259, Percent_Identity=23.1660231660232, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87081816, Length=239, Percent_Identity=23.8493723849372, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790436, Length=215, Percent_Identity=22.7906976744186, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789403, Length=224, Percent_Identity=27.2321428571429, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790861, Length=210, Percent_Identity=21.9047619047619, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789149, Length=232, Percent_Identity=24.5689655172414, Blast_Score=63, Evalue=5e-11,
Organism=Escherichia coli, GI87082128, Length=247, Percent_Identity=21.0526315789474, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PHOR_HAEIN (P71380)

Other databases:

- EMBL:   L42023
- RefSeq:   NP_439530.1
- GeneID:   950291
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1378
- NMPDR:   fig|71421.1.peg.1315
- TIGR:   HI_1378
- HOGENOM:   HBG334875
- OMA:   NIESTET
- ProtClustDB:   CLSK870092
- BioCyc:   HINF71421:HI_1378-MONOMER
- BRENDA:   2.7.13.3
- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR014310
- Gene3D:   G3DSA:3.30.565.10
- PRINTS:   PR00344
- SMART:   SM00387
- SMART:   SM00388
- SMART:   SM00091
- TIGRFAMs:   TIGR02966

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; SSF55874 ATP_bd_ATPase; SSF47384 His_kin_homodim

EC number: =2.7.13.3

Molecular weight: Translated: 49805; Mature: 49805

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS50109 HIS_KIN; PS50112 PAS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xb1c0bbc)-;

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILNFIVEINLAIIISLFTSDFILWFAIILLLILAWHHINEYRLLKYLNLKQDNKFSL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEE
LQLGTFSQTEAYHRHQIYKEKCASLRLLSQINKNIKYLPDAIIICQHNGNISWCNSIAPQ
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEEEEECCCCCHHHHHHHH
MFDFCWDKKVQENIFDVIFYEQFKHYFFSPKKRRPLVLLTYNQRYIEVQSHAYNSHMILV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEEEEECCCCCCEEEEE
IARDITDMIHLLNSRQKFLSNINHELRTPLTVLQGYLEILADNNIQNPLQKKAIXAMQEQ
EEHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
SQRMEHLLQQFNFLAKIETTSDKDFRKFDMSAMINSLRKDTDILNTYNHHIEFIIQPNII
HHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCEE
IFGNESQLRSAVSNLIYNAIKHSGKQCHIQIQWETCEQGIKFNVIDNGVGISPQHIPHLT
EECCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEHHHCCCEEEEEECCCCCCCHHHHHHH
ERFYRVDESRSHLTGGSGLGLAIVKHTLLQYHSHLNIESTETKGSSFSFIIPKRFVISKN
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCEEEEECC
NKEIQ
CCCCH
>Mature Secondary Structure
MKKILNFIVEINLAIIISLFTSDFILWFAIILLLILAWHHINEYRLLKYLNLKQDNKFSL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEE
LQLGTFSQTEAYHRHQIYKEKCASLRLLSQINKNIKYLPDAIIICQHNGNISWCNSIAPQ
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEEEEECCCCCHHHHHHHH
MFDFCWDKKVQENIFDVIFYEQFKHYFFSPKKRRPLVLLTYNQRYIEVQSHAYNSHMILV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEEEEECCCCCCEEEEE
IARDITDMIHLLNSRQKFLSNINHELRTPLTVLQGYLEILADNNIQNPLQKKAIXAMQEQ
EEHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
SQRMEHLLQQFNFLAKIETTSDKDFRKFDMSAMINSLRKDTDILNTYNHHIEFIIQPNII
HHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCEE
IFGNESQLRSAVSNLIYNAIKHSGKQCHIQIQWETCEQGIKFNVIDNGVGISPQHIPHLT
EECCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEHHHCCCEEEEEECCCCCCCHHHHHHH
ERFYRVDESRSHLTGGSGLGLAIVKHTLLQYHSHLNIESTETKGSSFSFIIPKRFVISKN
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCEEEEECC
NKEIQ
CCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800