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The map label for this gene is ttcA

Identifier: 16273282

GI number: 16273282

Start: 1462634

End: 1463575

Strand: Reverse

Name: ttcA

Synonym: HI1371.1

Alternate gene names: 16273282

Gene position: 1463575-1462634 (Counterclockwise)

Preceding gene: 16273289

Following gene: 16273281

Centisome position: 79.97

GC content: 37.58

Gene sequence:

>942_bases
ATGAGCGAATTAACCCAGTTAGCCCAACAAGAAAAAAAACAAACCTATAATTTCAATAAATTACAAAAACGTCTTCGCCG
TAATGTAGGCAACGCCATTGCAGATTTTGGTATGATCGAAGATGGCGATAAAGTCATGGTGTGTTTATCCGGAGGCAAAG
ACAGTTATACGCTTCTCGATATTTTGTTGAATTTACAGCAAAGTGCACCGATTAAATTTGATATCGTGGCAGTAAACCTC
GATCAAAAACAACCAGGGTTTCCAGAACACGTATTACCTGAATATTTAGAAAGCATTGGCGTCGATTACAAAATCGTACA
AGAAAATACTTACGGAATTGTGAAAGAAAAAATTCCAGAAGGAAAAACTACTTGTTCACTCTGCTCTCGCCTACGCCGTG
GAATTTTATATCGTACCGCCACAGAACTTGGTGCAACCAAAATCGCTTTAGGGCACCACCGAGACGATATGTTAGCGACA
CTATTTTTAAATATGTTTTATGGCGGCAAAATAAAATCCATGCCACCTAAATTGATTTCTGATGATGGCAAACAAATTGT
GATTCGTCCGCTTGCTTATTGCAAAGAAAAAGATATTGAGAAATATGCCATCGCGAAGGAATTTCCGATTATTCCTTGTA
ACCTATGTGGCTCACAACCAAATCTTCAACGCCAAGTCGTAAAAGAAATGCTTAATACTTGGGATCGTCAATATCCTGGA
CGCTTAGAAACTATGTTTAGCGCAATGCAAAACATTACATTGTCACATATGTGTGATCCGAAATTATTTGATTTCAAAGG
CATCAAACATGGGCAACTAATTGATGGCATTGAAGGCGATACCGCATTTGATGAAGAACGAATCACGCCAATGCAATTTG
AGGACGAAGATCAAACTGATTTTTCAAACAAGGAAATGATTAACTTTAAAGAAGTAAATTAA

Upstream 100 bases:

>100_bases
GATTTTTTGCGGATCTTAAAAATCTTCTTTTGAATTGACCGCACTTTTCCTATTTTTCACGTATAATGTGCGCCTTTCAT
GAATAACTAAAGATAACAGA

Downstream 100 bases:

>100_bases
TATGTTGAATATCAATGGAATTGAAGTTGAAACTGACAAAGACGGTTATTTGCTCCATAGCCAACAATGGAACGAAGATG
CCGCTCGTGCTATTGCACAG

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MSELTQLAQQEKKQTYNFNKLQKRLRRNVGNAIADFGMIEDGDKVMVCLSGGKDSYTLLDILLNLQQSAPIKFDIVAVNL
DQKQPGFPEHVLPEYLESIGVDYKIVQENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDMLAT
LFLNMFYGGKIKSMPPKLISDDGKQIVIRPLAYCKEKDIEKYAIAKEFPIIPCNLCGSQPNLQRQVVKEMLNTWDRQYPG
RLETMFSAMQNITLSHMCDPKLFDFKGIKHGQLIDGIEGDTAFDEERITPMQFEDEDQTDFSNKEMINFKEVN

Sequences:

>Translated_313_residues
MSELTQLAQQEKKQTYNFNKLQKRLRRNVGNAIADFGMIEDGDKVMVCLSGGKDSYTLLDILLNLQQSAPIKFDIVAVNL
DQKQPGFPEHVLPEYLESIGVDYKIVQENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDMLAT
LFLNMFYGGKIKSMPPKLISDDGKQIVIRPLAYCKEKDIEKYAIAKEFPIIPCNLCGSQPNLQRQVVKEMLNTWDRQYPG
RLETMFSAMQNITLSHMCDPKLFDFKGIKHGQLIDGIEGDTAFDEERITPMQFEDEDQTDFSNKEMINFKEVN
>Mature_312_residues
SELTQLAQQEKKQTYNFNKLQKRLRRNVGNAIADFGMIEDGDKVMVCLSGGKDSYTLLDILLNLQQSAPIKFDIVAVNLD
QKQPGFPEHVLPEYLESIGVDYKIVQENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDMLATL
FLNMFYGGKIKSMPPKLISDDGKQIVIRPLAYCKEKDIEKYAIAKEFPIIPCNLCGSQPNLQRQVVKEMLNTWDRQYPGR
LETMFSAMQNITLSHMCDPKLFDFKGIKHGQLIDGIEGDTAFDEERITPMQFEDEDQTDFSNKEMINFKEVN

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32)

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family

Homologues:

Organism=Escherichia coli, GI1787606, Length=299, Percent_Identity=77.257525083612, Blast_Score=482, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI193211090, Length=252, Percent_Identity=31.3492063492063, Blast_Score=135, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17540090, Length=237, Percent_Identity=24.8945147679325, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24651972, Length=222, Percent_Identity=27.9279279279279, Blast_Score=75, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TTCA_HAEIN (Q57184)

Other databases:

- EMBL:   L42023
- RefSeq:   NP_439523.1
- ProteinModelPortal:   Q57184
- SMR:   Q57184
- GeneID:   950672
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1371.1
- NMPDR:   fig|71421.1.peg.1308
- TIGR:   HI_1371.1
- HOGENOM:   HBG347038
- OMA:   EHILPEY
- ProtClustDB:   PRK10696
- BioCyc:   HINF71421:HI_1371.1-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01850
- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF004976

Pfam domain/function: PF01171 ATP_bind_3

EC number: NA

Molecular weight: Translated: 35704; Mature: 35572

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELTQLAQQEKKQTYNFNKLQKRLRRNVGNAIADFGMIEDGDKVMVCLSGGKDSYTLLD
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEECCCEEEEEEECCCCCHHHHH
ILLNLQQSAPIKFDIVAVNLDQKQPGFPEHVLPEYLESIGVDYKIVQENTYGIVKEKIPE
HHHHHHCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHCCC
GKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDMLATLFLNMFYGGKIKSMPPKLIS
CCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHC
DDGKQIVIRPLAYCKEKDIEKYAIAKEFPIIPCNLCGSQPNLQRQVVKEMLNTWDRQYPG
CCCCEEEEHHHHHHHHHCHHHHHHHHCCCCEEHHCCCCCCCHHHHHHHHHHHHHHHCCCC
RLETMFSAMQNITLSHMCDPKLFDFKGIKHGQLIDGIEGDTAFDEERITPMQFEDEDQTD
HHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCC
FSNKEMINFKEVN
CCCCCCCEEECCC
>Mature Secondary Structure 
SELTQLAQQEKKQTYNFNKLQKRLRRNVGNAIADFGMIEDGDKVMVCLSGGKDSYTLLD
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEECCCEEEEEEECCCCCHHHHH
ILLNLQQSAPIKFDIVAVNLDQKQPGFPEHVLPEYLESIGVDYKIVQENTYGIVKEKIPE
HHHHHHCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHCCC
GKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDMLATLFLNMFYGGKIKSMPPKLIS
CCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHC
DDGKQIVIRPLAYCKEKDIEKYAIAKEFPIIPCNLCGSQPNLQRQVVKEMLNTWDRQYPG
CCCCEEEEHHHHHHHHHCHHHHHHHHCCCCEEHHCCCCCCCHHHHHHHHHHHHHHHCCCC
RLETMFSAMQNITLSHMCDPKLFDFKGIKHGQLIDGIEGDTAFDEERITPMQFEDEDQTD
HHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCC
FSNKEMINFKEVN
CCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800