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The map label for this gene is phoB

Identifier: 16273290

GI number: 16273290

Start: 1475625

End: 1476320

Strand: Reverse

Name: phoB

Synonym: HI1379

Alternate gene names: 16273290

Gene position: 1476320-1475625 (Counterclockwise)

Preceding gene: 16273291

Following gene: 16273289

Centisome position: 80.67

GC content: 33.76

Gene sequence:

>696_bases
ATGACGAGAAAAATTCTGATAGTTGAAGATGAATGTGCAATCCGTGAAATGATCGCACTGTTTTTATCTCAAAAATACTA
TGATGTGATTGAAGCGAGCGATTTCAAAACAGCGATAAACAAGATAAAAGAAAATCCTAAACTAATATTATTGGATTGGA
TGCTACCTGGTCGTTCAGGTATACAATTTATACAGTATATTAAAAAGCAAGAAAGCTATGCTGCGATTCCCATTATTATG
CTCACAGCTAAAAGTACAGAAGAAGATTGTATTGCCTGTTTAAATGCAGGGGCTGATGACTACATCACTAAACCCTTTTC
ACCTCAAATTTTATTAGCTCGCATTGAGGCAGTATGGAGGAGAATTTATGAACAACAAAGTCAATTTATACAAATCGATG
AACTTTCCATTGATGAAAATGCTCAGAGAGTGTTTTTCCAACAGCAGGAAATTAATTTAAGTAGTACAGAATTTAAATTG
TTACATTTTTTTATGAGGCACCCTGAAAAAGTTTATTCTAGAGAACAATTGTTAAATCGTATTTGGCATAATGATCTAGA
AGTTGAATATCGCACTGTTGATAGCTATATTCGCCGTTTACGCCGTAATTTAGCCCCTTTTCAATGTGAACATTACATCC
AAACAGTTCGAGGATCTGGTTATCGATTTTCTAGTTATTTACGAGATAAACAATGA

Upstream 100 bases:

>100_bases
GGGTAGTTATTTTGAGTTCTCTTATTAAAAAGTAAAAGTTTGGTGAGAATGTATTATTTCTCACTTCACTTTTATGCCAT
AAATCAAATGAAGGAGTATT

Downstream 100 bases:

>100_bases
AAAAAATATTGAATTTTATTGTTGAAATAAATTTGGCAATCATTATTTCCTTATTTACTTCTGATTTTATTCTTTGGTTT
GCAATAATACTTTTATTAAT

Product: phosphate regulon transcriptional regulatory protein PhoB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MTRKILIVEDECAIREMIALFLSQKYYDVIEASDFKTAINKIKENPKLILLDWMLPGRSGIQFIQYIKKQESYAAIPIIM
LTAKSTEEDCIACLNAGADDYITKPFSPQILLARIEAVWRRIYEQQSQFIQIDELSIDENAQRVFFQQQEINLSSTEFKL
LHFFMRHPEKVYSREQLLNRIWHNDLEVEYRTVDSYIRRLRRNLAPFQCEHYIQTVRGSGYRFSSYLRDKQ

Sequences:

>Translated_231_residues
MTRKILIVEDECAIREMIALFLSQKYYDVIEASDFKTAINKIKENPKLILLDWMLPGRSGIQFIQYIKKQESYAAIPIIM
LTAKSTEEDCIACLNAGADDYITKPFSPQILLARIEAVWRRIYEQQSQFIQIDELSIDENAQRVFFQQQEINLSSTEFKL
LHFFMRHPEKVYSREQLLNRIWHNDLEVEYRTVDSYIRRLRRNLAPFQCEHYIQTVRGSGYRFSSYLRDKQ
>Mature_230_residues
TRKILIVEDECAIREMIALFLSQKYYDVIEASDFKTAINKIKENPKLILLDWMLPGRSGIQFIQYIKKQESYAAIPIIML
TAKSTEEDCIACLNAGADDYITKPFSPQILLARIEAVWRRIYEQQSQFIQIDELSIDENAQRVFFQQQEINLSSTEFKLL
HFFMRHPEKVYSREQLLNRIWHNDLEVEYRTVDSYIRRLRRNLAPFQCEHYIQTVRGSGYRFSSYLRDKQ

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1786599, Length=227, Percent_Identity=53.3039647577093, Blast_Score=256, Evalue=1e-69,
Organism=Escherichia coli, GI1788394, Length=228, Percent_Identity=37.719298245614, Blast_Score=140, Evalue=9e-35,
Organism=Escherichia coli, GI1786911, Length=225, Percent_Identity=34.2222222222222, Blast_Score=127, Evalue=6e-31,
Organism=Escherichia coli, GI1789809, Length=229, Percent_Identity=34.061135371179, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI2367329, Length=231, Percent_Identity=34.1991341991342, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1790860, Length=221, Percent_Identity=32.1266968325792, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI87082012, Length=222, Percent_Identity=34.2342342342342, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI1786784, Length=224, Percent_Identity=33.4821428571429, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1787375, Length=221, Percent_Identity=29.4117647058824, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1790863, Length=239, Percent_Identity=32.2175732217573, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1790552, Length=219, Percent_Identity=32.4200913242009, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI145693140, Length=236, Percent_Identity=30.9322033898305, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1789402, Length=222, Percent_Identity=27.9279279279279, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1787229, Length=236, Percent_Identity=29.6610169491525, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788191, Length=121, Percent_Identity=28.099173553719, Blast_Score=74, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PHOB_HAEIN (P45189)

Other databases:

- EMBL:   L42023
- PIR:   F64120
- RefSeq:   NP_439531.1
- ProteinModelPortal:   P45189
- SMR:   P45189
- GeneID:   950671
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1379
- TIGR:   HI_1379
- HOGENOM:   HBG753323
- OMA:   NDIYVED
- ProtClustDB:   CLSK870093
- BioCyc:   HINF71421:HI_1379-MONOMER
- GO:   GO:0005737
- GO:   GO:0006350
- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- SMART:   SM00448
- SMART:   SM00862
- TIGRFAMs:   TIGR02154

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C; SSF52172 CheY_like

EC number: NA

Molecular weight: Translated: 27508; Mature: 27377

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRKILIVEDECAIREMIALFLSQKYYDVIEASDFKTAINKIKENPKLILLDWMLPGRSG
CCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCH
IQFIQYIKKQESYAAIPIIMLTAKSTEEDCIACLNAGADDYITKPFSPQILLARIEAVWR
HHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
RIYEQQSQFIQIDELSIDENAQRVFFQQQEINLSSTEFKLLHFFMRHPEKVYSREQLLNR
HHHHHHHHEEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHH
IWHNDLEVEYRTVDSYIRRLRRNLAPFQCEHYIQTVRGSGYRFSSYLRDKQ
HCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHCCCC
>Mature Secondary Structure 
TRKILIVEDECAIREMIALFLSQKYYDVIEASDFKTAINKIKENPKLILLDWMLPGRSG
CCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCH
IQFIQYIKKQESYAAIPIIMLTAKSTEEDCIACLNAGADDYITKPFSPQILLARIEAVWR
HHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
RIYEQQSQFIQIDELSIDENAQRVFFQQQEINLSSTEFKLLHFFMRHPEKVYSREQLLNR
HHHHHHHHEEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHH
IWHNDLEVEYRTVDSYIRRLRRNLAPFQCEHYIQTVRGSGYRFSSYLRDKQ
HCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800