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The map label for this gene is pntB

Identifier: 16273273

GI number: 16273273

Start: 1448434

End: 1449858

Strand: Direct

Name: pntB

Synonym: HI1363

Alternate gene names: 16273273

Gene position: 1448434-1449858 (Clockwise)

Preceding gene: 16273272

Following gene: 16273275

Centisome position: 79.14

GC content: 41.68

Gene sequence:

>1425_bases
ATGTCTGAAGGTTTAGTACAGGCTGCGTACATTTTAGCAGCATTACTTTTCATTATGAGCTTGGCAGGACTTTCTAAACA
TGAAACTGCTAAAGCGGGTTGTTGGTTCGGTATTGTTGGTATGACAATTGCCCTTATTGCAACCATTTTTGGCCCTCATT
CTGAAGGAACATTTTGGATCATTATTGCGATGATCATTGGTGGAGCAATCGGTGTTCAACGTGCATTAAAAGTAGAAATG
ACTGAAATGCCAGAACTTGTGGCGATTCTTCACAGTTTTGTTGGTTTAGCGGCTGTACTCGTTGGCTTTAATAGCTATGG
TTTACACCACGAGGCGTTAATGCCAGAAGGATTAGATGCAGCAGCACAAGCCGCATTTGTAGCTGAACAAGCGGTATTAA
CGAATATTCATAATGTCGAAGTCTTTTTAGGTATTTTTATCGGTGCGGTAACTTTCACTGGTTCTGTTGTTGCATTTGGT
AAATTAAGCGGAAAAATTAATTCAAAAGCATTAATGTTACCGCATCGCCATAAATTAAATTTAGCCGCACTTGTGGTATC
TGCATTATTGATGGTTGCATTCTTGAATAATCCAGAAAGCATTTTCCCTGTGTTATTAATGACAGCGATTGCGCTTGCAT
TTGGATGGCACTTAGTGGCATCAATCGGTGGGGCAGATATGCCAGTTGTAGTATCAATGCTTAACTCATATTCTGGTTGG
GCAGCAGCTGCAGCAGGTTTTATATTGAATAATGATTTGCTCATCGTTACTGGTGCGCTTGTTGGTTCTTCTGGTGCAAT
TCTTTCTTACATTATGTGTAAAGCGATGAACCGTTCATTTGTGAGCGTCATTGCTGGTGGTTTTGGTAACGATGTTCAAG
TTTCTTCAAGCGAAGAACAAGGCGAACACCGTGAAACTACAGCGGAAGAAGTGGCTGAATTGTTGAAAAATGCAAGTTCT
GTGATTATCACGCCGGGATATGGTATGGCTGTTGCACAAGCACAATATCCAGTCGCTGATATTACGGCAAAATTGCGTGA
ACGTGGTGTGAACGTTCGTTTTGGTATTCACCCTGTTGCAGGACGTTTACCGGGCCACATGAACGTGCTTTTAGCTGAAG
CGAAAGTACCTTACGATGTCGTACTTGAAATGGATGAAATCAATGATGATTTCGCTGATACCGATGTGGTATTGGTTATA
GGTGCGAACGATACGGTAAACCCAGCGGCGATGGAAGATCCAAATAGCCCAATCGCGGGAATGCCTGTGTTAGAAGTATG
GAAAGCACAAAATGTGATTGTGTTCAAACGTTCAATGGCAGTGGGTTACGCTGGCGTACAAAACCCATTATTCTTCAAAG
AGAATACGCAAATGCTCTTTGGTGATGCAAAAGATCGCGTTGAAGATATCTTAAAAGCATTATAA

Upstream 100 bases:

>100_bases
GCTTTTGTGGCGATTTTGGTGGCAAGCATCAATATTTTTGGTGGTTTCCGTGTCACACAACGTATGTTAGCAATGTTTAG
AAAAGGTTAAGGAGCGCACA

Downstream 100 bases:

>100_bases
AAAACACGCATAAAAATAACCGCACTTTGGGTTGCTTAAGTGCGGTATTTTTATTTATAGCATTTAAATATTATCTTCAT
GATTTTCCAAAATTTCTGAA

Product: pyridine nucleotide transhydrogenase

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit beta; Pyridine nucleotide transhydrogenase subunit beta

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MSEGLVQAAYILAALLFIMSLAGLSKHETAKAGCWFGIVGMTIALIATIFGPHSEGTFWIIIAMIIGGAIGVQRALKVEM
TEMPELVAILHSFVGLAAVLVGFNSYGLHHEALMPEGLDAAAQAAFVAEQAVLTNIHNVEVFLGIFIGAVTFTGSVVAFG
KLSGKINSKALMLPHRHKLNLAALVVSALLMVAFLNNPESIFPVLLMTAIALAFGWHLVASIGGADMPVVVSMLNSYSGW
AAAAAGFILNNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGNDVQVSSSEEQGEHRETTAEEVAELLKNASS
VIITPGYGMAVAQAQYPVADITAKLRERGVNVRFGIHPVAGRLPGHMNVLLAEAKVPYDVVLEMDEINDDFADTDVVLVI
GANDTVNPAAMEDPNSPIAGMPVLEVWKAQNVIVFKRSMAVGYAGVQNPLFFKENTQMLFGDAKDRVEDILKAL

Sequences:

>Translated_474_residues
MSEGLVQAAYILAALLFIMSLAGLSKHETAKAGCWFGIVGMTIALIATIFGPHSEGTFWIIIAMIIGGAIGVQRALKVEM
TEMPELVAILHSFVGLAAVLVGFNSYGLHHEALMPEGLDAAAQAAFVAEQAVLTNIHNVEVFLGIFIGAVTFTGSVVAFG
KLSGKINSKALMLPHRHKLNLAALVVSALLMVAFLNNPESIFPVLLMTAIALAFGWHLVASIGGADMPVVVSMLNSYSGW
AAAAAGFILNNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGNDVQVSSSEEQGEHRETTAEEVAELLKNASS
VIITPGYGMAVAQAQYPVADITAKLRERGVNVRFGIHPVAGRLPGHMNVLLAEAKVPYDVVLEMDEINDDFADTDVVLVI
GANDTVNPAAMEDPNSPIAGMPVLEVWKAQNVIVFKRSMAVGYAGVQNPLFFKENTQMLFGDAKDRVEDILKAL
>Mature_473_residues
SEGLVQAAYILAALLFIMSLAGLSKHETAKAGCWFGIVGMTIALIATIFGPHSEGTFWIIIAMIIGGAIGVQRALKVEMT
EMPELVAILHSFVGLAAVLVGFNSYGLHHEALMPEGLDAAAQAAFVAEQAVLTNIHNVEVFLGIFIGAVTFTGSVVAFGK
LSGKINSKALMLPHRHKLNLAALVVSALLMVAFLNNPESIFPVLLMTAIALAFGWHLVASIGGADMPVVVSMLNSYSGWA
AAAAGFILNNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGNDVQVSSSEEQGEHRETTAEEVAELLKNASSV
IITPGYGMAVAQAQYPVADITAKLRERGVNVRFGIHPVAGRLPGHMNVLLAEAKVPYDVVLEMDEINDDFADTDVVLVIG
ANDTVNPAAMEDPNSPIAGMPVLEVWKAQNVIVFKRSMAVGYAGVQNPLFFKENTQMLFGDAKDRVEDILKAL

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane

COG id: COG1282

COG function: function code C; NAD/NADP transhydrogenase beta subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNT beta subunit family

Homologues:

Organism=Homo sapiens, GI122939155, Length=474, Percent_Identity=50.42194092827, Blast_Score=449, Evalue=1e-126,
Organism=Homo sapiens, GI122939153, Length=474, Percent_Identity=50.42194092827, Blast_Score=449, Evalue=1e-126,
Organism=Escherichia coli, GI1787886, Length=478, Percent_Identity=77.8242677824268, Blast_Score=726, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17550456, Length=471, Percent_Identity=48.4076433121019, Blast_Score=397, Evalue=1e-111,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PNTB_HAEIN (P43010)

Other databases:

- EMBL:   L42023
- EMBL:   U20964
- PIR:   F64119
- RefSeq:   NP_439514.1
- ProteinModelPortal:   P43010
- SMR:   P43010
- GeneID:   950280
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1363
- NMPDR:   fig|71421.1.peg.1299
- TIGR:   HI_1363
- HOGENOM:   HBG648813
- OMA:   HHEALMP
- ProtClustDB:   PRK09444
- BioCyc:   HINF71421:HI_1363-MONOMER
- BRENDA:   1.6.1.2
- InterPro:   IPR012136
- InterPro:   IPR004003
- PIRSF:   PIRSF000204

Pfam domain/function: PF02233 PNTB

EC number: =1.6.1.2

Molecular weight: Translated: 50163; Mature: 50032

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xe648e24)-; HASH(0xe39b4b4)-; HASH(0xe684db8)-; HASH(0xd7f5778)-; HASH(0xde60f54)-; HASH(0xe606d40)-; HASH(0xd4f24a4)-; HASH(0xe71eba8)-; HASH(0xafa5f50)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEGLVQAAYILAALLFIMSLAGLSKHETAKAGCWFGIVGMTIALIATIFGPHSEGTFWI
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHH
IIAMIIGGAIGVQRALKVEMTEMPELVAILHSFVGLAAVLVGFNSYGLHHEALMPEGLDA
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCH
AAQAAFVAEQAVLTNIHNVEVFLGIFIGAVTFTGSVVAFGKLSGKINSKALMLPHRHKLN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCCCCCH
LAALVVSALLMVAFLNNPESIFPVLLMTAIALAFGWHLVASIGGADMPVVVSMLNSYSGW
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCH
AAAAAGFILNNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGNDVQVSSSEEQ
HHHHHHHHCCCCEEEEEEEHHCCCCHHHHHHHHHHHCHHHHHHHHCCCCCCEEECCCHHC
GEHRETTAEEVAELLKNASSVIITPGYGMAVAQAQYPVADITAKLRERGVNVRFGIHPVA
CCHHHHHHHHHHHHHHCCCCEEEECCCCCEEHHCCCCHHHHHHHHHHCCCCEEEECCHHC
GRLPGHMNVLLAEAKVPYDVVLEMDEINDDFADTDVVLVIGANDTVNPAAMEDPNSPIAG
CCCCCCCEEEEECCCCCHHHEEEHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCC
MPVLEVWKAQNVIVFKRSMAVGYAGVQNPLFFKENTQMLFGDAKDRVEDILKAL
CCHHHHHCCCCEEEEEEHHHHCCCCCCCCEEEECCCCEEECCHHHHHHHHHHHH
>Mature Secondary Structure 
SEGLVQAAYILAALLFIMSLAGLSKHETAKAGCWFGIVGMTIALIATIFGPHSEGTFWI
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHH
IIAMIIGGAIGVQRALKVEMTEMPELVAILHSFVGLAAVLVGFNSYGLHHEALMPEGLDA
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCH
AAQAAFVAEQAVLTNIHNVEVFLGIFIGAVTFTGSVVAFGKLSGKINSKALMLPHRHKLN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCCCCCH
LAALVVSALLMVAFLNNPESIFPVLLMTAIALAFGWHLVASIGGADMPVVVSMLNSYSGW
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCH
AAAAAGFILNNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGNDVQVSSSEEQ
HHHHHHHHCCCCEEEEEEEHHCCCCHHHHHHHHHHHCHHHHHHHHCCCCCCEEECCCHHC
GEHRETTAEEVAELLKNASSVIITPGYGMAVAQAQYPVADITAKLRERGVNVRFGIHPVA
CCHHHHHHHHHHHHHHCCCCEEEECCCCCEEHHCCCCHHHHHHHHHHCCCCEEEECCHHC
GRLPGHMNVLLAEAKVPYDVVLEMDEINDDFADTDVVLVIGANDTVNPAAMEDPNSPIAG
CCCCCCCEEEEECCCCCHHHEEEHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCC
MPVLEVWKAQNVIVFKRSMAVGYAGVQNPLFFKENTQMLFGDAKDRVEDILKAL
CCHHHHHCCCCEEEEEEHHHHCCCCCCCCEEEECCCCEEECCHHHHHHHHHHHH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800; 8635745