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The map label for this gene is topA [H]

Identifier: 16273275

GI number: 16273275

Start: 1450938

End: 1453544

Strand: Direct

Name: topA [H]

Synonym: HI1365

Alternate gene names: 16273275

Gene position: 1450938-1453544 (Clockwise)

Preceding gene: 16273273

Following gene: 16273277

Centisome position: 79.28

GC content: 39.16

Gene sequence:

>2607_bases
ATGAGTAAATCCTTGGTGATTGTGGAGTCGCCAGCAAAAGCAAAAACTATTAATAAGTATTTAGGTAGCCAATATGTCGT
GAAATCGAGCGTGGGACATATTCGTGATTTGCCGACGGTTGGTTCTAGTACAGGTGAAAAAGCTAAACCAATTTCAACCA
AAGGTATGGATGCCGAAGAAAAAGCAAAAATTAAGGCAGAAAAGGAACGTAACGCCTTAGTTAAACGTATGGGGATTGAT
CCTTATCACGATTGGAAAGCAAATTATCAGATTTTGCCTGGTAAAGAAAAAGTGGTTTCCGAGTTAAAGTCGCTTGCTAA
AAAAGCAGATCACATTTATCTCGCAACCGACTTGGATAGAGAAGGGGAAGCGATTGCGTGGCATTTACGTGAAGTGATTG
GTGGTAACGATGATCGTTTTAGTCGCGTGGTGTTTAACGAAATCACTAAAAATGCCATTAAACAGGCTTTTGAAAAGCCT
GAACAATTAAATATGGATCGTGTTAATGCTCAACAAACTCGTCGTTTTTTAGATAGAGTGGTGGGCTTTATGGTGTCGCC
ATTACTTTGGAAAAAAGTAGCTCGCGGGCTTTCTGCTGGTCGAGTTCAATCAGTTGCGGTTAAATTATTAGTCGAACGCG
AGCGTGAAATTAAAGCGTTCCAGCCTGAAGAATATTGGGAAGTTGCCGTCCTTACGAATAATCAAAATAAACAAGCTATA
CGTTTAGATGTTACGGATTATAAAGGCAAGAAATTTGATCCAAAAAATCAAAAAGAAGCACAAAGTGCGGTAGATTTTCT
GAATGTTTCAGATTACGTTGTCACAGATTTGGAAACTAAGCCAACAAGTTCTCGCCCTCGAGCACCATTTATTACATCAA
CACTTCAACAAACGGCAAGTACTCGCTTGGGATTTGGCGTTAAGAAGACAATGATGTTAGCCCAACGTTTATATGAAGCG
GGTTACATTACTTATATGCGTACGGATTCAACCAATTTGAGTCAAGATGCGCTTAATATGGCGCGTAGCTATATTGAAAA
TCATTTTGGTGCACAATATTTACCAGAAAAACCAAATTTCTATTCTAGTAAAGAAAATGCGCAAGAGGCACATGAGGCTA
TTCGTCCATCAGATATTCGAGCATTGCCTGAATCTTTAGAGGGAATGGAAAAAGATGCGGTACGCCTTTATGATTTAATT
TGGTGTCAATTCTTAGCATGTCAAATGCCACCTGCACAATATGATAGTAGCACGCTGACCGTTACCGCTGGCGATTATAC
CTTGAAAGCAAAGGGCAGAATTTTACGTTTTGATGGTTGGACAAAAGTATTGCCACAGATAGGCAAAAATCCTGAAGATC
AAGAATTGCCTTCAGTTACTGTGTCAGAAAAACTTGCTTTAAAAGAAGTGCAACCAACTCAACATTTTACTAAACCACCT
GCTCGTTTTACTGAAGCGGCTTTAGTAAAAGAATTGGAAAAACGTGGTATTGGCAGACCTTCCACTTATGCGGCAATTAT
TTCCACCATTCAAGAACGAGGTTATGTTCGTACTGAAAATCGTCGTTTCTATGCAGAAAAGATGGGCGAAATTGTGACTG
ATCGCCTCAATGAGTCTTTTGGTGAATTAATGAATTACGATTTCACCGCGAATATGGAAGATACCTTAGATAAAATTGCC
TCTGGTTCAGTAAATTGGAAAACTGAATTGAATCAATTCTTTAAGGATTTTTCTAGCCAGCTTTCTAAAGCTGAATTAGA
TGAACTTGAAGGTGGTATGCGCCCGAATAGCCTTGTGGAAACAGATATTAAATGCCCAACCTGTGGCAGAAATATGGCAA
TTCGTACGGCGAGTACGGGGGTGTTTTTAGGATGTACAGGTTATGCATTACCACCAAAAGAGCGGTGTAAAACGACGATT
AATTTAATTCCTGAAGCGGAATTATTGAATGTTTTAGATGAATCTTCAGAAACCAAAGCATTAATGGATCGTAAACGTTG
TACAAAATGTGGAACGGCAATGGACAGCTATGTAATCGATGCCCATCGTAAAATCCATATTTGCGGTAATAATCCAAATT
GTGATGGATATTTAATTGAAGAAGGCTCATTCAAAATTAAAGGTTATGATGGCCCTGTTGTTGAGTGTGATAAATGCGGT
GCGGATATGCATCTAAAACTTGGTCGTTTTGGCAAGTATATGGGATGTACAAATTGTGATAACACACGCAAAATTTTGAA
AAATGGCGAGGTCGCACCACCGAAAGAAGAGCCAGTACATTTCCCTGAGTTAAAATGCGAAAAATCTGATGCATATTTTG
TATTACGTGATGGTGCGAGTGGTGTATTTATGTCAGCCCATAACTTCCCTAAATCACGCGAAACACGCCCAGTTAAAATC
GCTGAACTAGTGCAATATCGCGAGCGTTTACCTGAAAAATTAGCTTATTTAGCCGATGCTCCACAAAAAGACCCAGAAGA
GAATGAAGCGATTGTTCGTTTTAGTCGTAAAGAGAAGAAACAATATGTGACTTCTGAAAAAGAGGGAAAAGCGACGAAAT
GGATTGTTGATTTCACCAATGGAAAATGGGTTGAACGAAAGAAATAA

Upstream 100 bases:

>100_bases
TCCATTTATAGACCTTTTTTGAAAAAATGCTTGATTTTGCCAACATAACTTTGCACTATACCGCCAATTTTACAAAATTC
TAGTAATATTAGGTAATCAA

Downstream 100 bases:

>100_bases
GAAAAAAAGTGCGGTAAAATCACCGCACTTTTTGTTTGTAATAAATTTTATTATGTTTTGTAGATTATCTGAGGATTAAA
GGGCTGCTACAATTTCATTA

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 868; Mature: 867

Protein sequence:

>868_residues
MSKSLVIVESPAKAKTINKYLGSQYVVKSSVGHIRDLPTVGSSTGEKAKPISTKGMDAEEKAKIKAEKERNALVKRMGID
PYHDWKANYQILPGKEKVVSELKSLAKKADHIYLATDLDREGEAIAWHLREVIGGNDDRFSRVVFNEITKNAIKQAFEKP
EQLNMDRVNAQQTRRFLDRVVGFMVSPLLWKKVARGLSAGRVQSVAVKLLVEREREIKAFQPEEYWEVAVLTNNQNKQAI
RLDVTDYKGKKFDPKNQKEAQSAVDFLNVSDYVVTDLETKPTSSRPRAPFITSTLQQTASTRLGFGVKKTMMLAQRLYEA
GYITYMRTDSTNLSQDALNMARSYIENHFGAQYLPEKPNFYSSKENAQEAHEAIRPSDIRALPESLEGMEKDAVRLYDLI
WCQFLACQMPPAQYDSSTLTVTAGDYTLKAKGRILRFDGWTKVLPQIGKNPEDQELPSVTVSEKLALKEVQPTQHFTKPP
ARFTEAALVKELEKRGIGRPSTYAAIISTIQERGYVRTENRRFYAEKMGEIVTDRLNESFGELMNYDFTANMEDTLDKIA
SGSVNWKTELNQFFKDFSSQLSKAELDELEGGMRPNSLVETDIKCPTCGRNMAIRTASTGVFLGCTGYALPPKERCKTTI
NLIPEAELLNVLDESSETKALMDRKRCTKCGTAMDSYVIDAHRKIHICGNNPNCDGYLIEEGSFKIKGYDGPVVECDKCG
ADMHLKLGRFGKYMGCTNCDNTRKILKNGEVAPPKEEPVHFPELKCEKSDAYFVLRDGASGVFMSAHNFPKSRETRPVKI
AELVQYRERLPEKLAYLADAPQKDPEENEAIVRFSRKEKKQYVTSEKEGKATKWIVDFTNGKWVERKK

Sequences:

>Translated_868_residues
MSKSLVIVESPAKAKTINKYLGSQYVVKSSVGHIRDLPTVGSSTGEKAKPISTKGMDAEEKAKIKAEKERNALVKRMGID
PYHDWKANYQILPGKEKVVSELKSLAKKADHIYLATDLDREGEAIAWHLREVIGGNDDRFSRVVFNEITKNAIKQAFEKP
EQLNMDRVNAQQTRRFLDRVVGFMVSPLLWKKVARGLSAGRVQSVAVKLLVEREREIKAFQPEEYWEVAVLTNNQNKQAI
RLDVTDYKGKKFDPKNQKEAQSAVDFLNVSDYVVTDLETKPTSSRPRAPFITSTLQQTASTRLGFGVKKTMMLAQRLYEA
GYITYMRTDSTNLSQDALNMARSYIENHFGAQYLPEKPNFYSSKENAQEAHEAIRPSDIRALPESLEGMEKDAVRLYDLI
WCQFLACQMPPAQYDSSTLTVTAGDYTLKAKGRILRFDGWTKVLPQIGKNPEDQELPSVTVSEKLALKEVQPTQHFTKPP
ARFTEAALVKELEKRGIGRPSTYAAIISTIQERGYVRTENRRFYAEKMGEIVTDRLNESFGELMNYDFTANMEDTLDKIA
SGSVNWKTELNQFFKDFSSQLSKAELDELEGGMRPNSLVETDIKCPTCGRNMAIRTASTGVFLGCTGYALPPKERCKTTI
NLIPEAELLNVLDESSETKALMDRKRCTKCGTAMDSYVIDAHRKIHICGNNPNCDGYLIEEGSFKIKGYDGPVVECDKCG
ADMHLKLGRFGKYMGCTNCDNTRKILKNGEVAPPKEEPVHFPELKCEKSDAYFVLRDGASGVFMSAHNFPKSRETRPVKI
AELVQYRERLPEKLAYLADAPQKDPEENEAIVRFSRKEKKQYVTSEKEGKATKWIVDFTNGKWVERKK
>Mature_867_residues
SKSLVIVESPAKAKTINKYLGSQYVVKSSVGHIRDLPTVGSSTGEKAKPISTKGMDAEEKAKIKAEKERNALVKRMGIDP
YHDWKANYQILPGKEKVVSELKSLAKKADHIYLATDLDREGEAIAWHLREVIGGNDDRFSRVVFNEITKNAIKQAFEKPE
QLNMDRVNAQQTRRFLDRVVGFMVSPLLWKKVARGLSAGRVQSVAVKLLVEREREIKAFQPEEYWEVAVLTNNQNKQAIR
LDVTDYKGKKFDPKNQKEAQSAVDFLNVSDYVVTDLETKPTSSRPRAPFITSTLQQTASTRLGFGVKKTMMLAQRLYEAG
YITYMRTDSTNLSQDALNMARSYIENHFGAQYLPEKPNFYSSKENAQEAHEAIRPSDIRALPESLEGMEKDAVRLYDLIW
CQFLACQMPPAQYDSSTLTVTAGDYTLKAKGRILRFDGWTKVLPQIGKNPEDQELPSVTVSEKLALKEVQPTQHFTKPPA
RFTEAALVKELEKRGIGRPSTYAAIISTIQERGYVRTENRRFYAEKMGEIVTDRLNESFGELMNYDFTANMEDTLDKIAS
GSVNWKTELNQFFKDFSSQLSKAELDELEGGMRPNSLVETDIKCPTCGRNMAIRTASTGVFLGCTGYALPPKERCKTTIN
LIPEAELLNVLDESSETKALMDRKRCTKCGTAMDSYVIDAHRKIHICGNNPNCDGYLIEEGSFKIKGYDGPVVECDKCGA
DMHLKLGRFGKYMGCTNCDNTRKILKNGEVAPPKEEPVHFPELKCEKSDAYFVLRDGASGVFMSAHNFPKSRETRPVKIA
ELVQYRERLPEKLAYLADAPQKDPEENEAIVRFSRKEKKQYVTSEKEGKATKWIVDFTNGKWVERKK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=597, Percent_Identity=26.8006700167504, Blast_Score=142, Evalue=2e-33,
Organism=Homo sapiens, GI4507635, Length=550, Percent_Identity=25.2727272727273, Blast_Score=138, Evalue=2e-32,
Organism=Escherichia coli, GI1787529, Length=871, Percent_Identity=71.6417910447761, Blast_Score=1316, Evalue=0.0,
Organism=Escherichia coli, GI1788061, Length=541, Percent_Identity=23.2902033271719, Blast_Score=96, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI32563869, Length=594, Percent_Identity=26.0942760942761, Blast_Score=132, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17555378, Length=502, Percent_Identity=25.4980079681275, Blast_Score=123, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6323263, Length=485, Percent_Identity=26.1855670103093, Blast_Score=130, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24585251, Length=624, Percent_Identity=23.7179487179487, Blast_Score=129, Evalue=7e-30,
Organism=Drosophila melanogaster, GI24640096, Length=500, Percent_Identity=24.6, Blast_Score=120, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR013263
- InterPro:   IPR006171 [H]

Pfam domain/function: PF08272 Topo_Zn_Ribbon; PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 98131; Mature: 98000

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSLVIVESPAKAKTINKYLGSQYVVKSSVGHIRDLPTVGSSTGEKAKPISTKGMDAEE
CCCCEEEEECCCHHHHHHHHHCCHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCHHH
KAKIKAEKERNALVKRMGIDPYHDWKANYQILPGKEKVVSELKSLAKKADHIYLATDLDR
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHCCEEEEEECCCC
EGEAIAWHLREVIGGNDDRFSRVVFNEITKNAIKQAFEKPEQLNMDRVNAQQTRRFLDRV
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHCCHHHHHHHHHHH
VGFMVSPLLWKKVARGLSAGRVQSVAVKLLVEREREIKAFQPEEYWEVAVLTNNQNKQAI
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEE
RLDVTDYKGKKFDPKNQKEAQSAVDFLNVSDYVVTDLETKPTSSRPRAPFITSTLQQTAS
EEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHH
TRLGFGVKKTMMLAQRLYEAGYITYMRTDSTNLSQDALNMARSYIENHFGAQYLPEKPNF
HHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
YSSKENAQEAHEAIRPSDIRALPESLEGMEKDAVRLYDLIWCQFLACQMPPAQYDSSTLT
CCCCHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
VTAGDYTLKAKGRILRFDGWTKVLPQIGKNPEDQELPSVTVSEKLALKEVQPTQHFTKPP
EEECCEEEEECCCEEEECCHHHHHHHHCCCCCCHHCCCCCHHHHHHHHHCCCHHHCCCCC
ARFTEAALVKELEKRGIGRPSTYAAIISTIQERGYVRTENRRFYAEKMGEIVTDRLNESF
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHH
GELMNYDFTANMEDTLDKIASGSVNWKTELNQFFKDFSSQLSKAELDELEGGMRPNSLVE
HHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
TDIKCPTCGRNMAIRTASTGVFLGCTGYALPPKERCKTTINLIPEAELLNVLDESSETKA
CCCCCCCCCCCCEEEEECCCEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHCCCCHHHH
LMDRKRCTKCGTAMDSYVIDAHRKIHICGNNPNCDGYLIEEGSFKIKGYDGPVVECDKCG
HHHHHHHHHHHHHHHHHEEECCCEEEEECCCCCCCEEEEECCCEEEECCCCCEEEHHHCC
ADMHLKLGRFGKYMGCTNCDNTRKILKNGEVAPPKEEPVHFPELKCEKSDAYFVLRDGAS
CCCEEEHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCEECCCCCEEEEECCCC
GVFMSAHNFPKSRETRPVKIAELVQYRERLPEKLAYLADAPQKDPEENEAIVRFSRKEKK
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
QYVTSEKEGKATKWIVDFTNGKWVERKK
HHHCCCCCCCCEEEEEECCCCCEECCCC
>Mature Secondary Structure 
SKSLVIVESPAKAKTINKYLGSQYVVKSSVGHIRDLPTVGSSTGEKAKPISTKGMDAEE
CCCEEEEECCCHHHHHHHHHCCHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCHHH
KAKIKAEKERNALVKRMGIDPYHDWKANYQILPGKEKVVSELKSLAKKADHIYLATDLDR
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHCCEEEEEECCCC
EGEAIAWHLREVIGGNDDRFSRVVFNEITKNAIKQAFEKPEQLNMDRVNAQQTRRFLDRV
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHCCHHHHHHHHHHH
VGFMVSPLLWKKVARGLSAGRVQSVAVKLLVEREREIKAFQPEEYWEVAVLTNNQNKQAI
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEE
RLDVTDYKGKKFDPKNQKEAQSAVDFLNVSDYVVTDLETKPTSSRPRAPFITSTLQQTAS
EEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHH
TRLGFGVKKTMMLAQRLYEAGYITYMRTDSTNLSQDALNMARSYIENHFGAQYLPEKPNF
HHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
YSSKENAQEAHEAIRPSDIRALPESLEGMEKDAVRLYDLIWCQFLACQMPPAQYDSSTLT
CCCCHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
VTAGDYTLKAKGRILRFDGWTKVLPQIGKNPEDQELPSVTVSEKLALKEVQPTQHFTKPP
EEECCEEEEECCCEEEECCHHHHHHHHCCCCCCHHCCCCCHHHHHHHHHCCCHHHCCCCC
ARFTEAALVKELEKRGIGRPSTYAAIISTIQERGYVRTENRRFYAEKMGEIVTDRLNESF
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHH
GELMNYDFTANMEDTLDKIASGSVNWKTELNQFFKDFSSQLSKAELDELEGGMRPNSLVE
HHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
TDIKCPTCGRNMAIRTASTGVFLGCTGYALPPKERCKTTINLIPEAELLNVLDESSETKA
CCCCCCCCCCCCEEEEECCCEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHCCCCHHHH
LMDRKRCTKCGTAMDSYVIDAHRKIHICGNNPNCDGYLIEEGSFKIKGYDGPVVECDKCG
HHHHHHHHHHHHHHHHHEEECCCEEEEECCCCCCCEEEEECCCEEEECCCCCEEEHHHCC
ADMHLKLGRFGKYMGCTNCDNTRKILKNGEVAPPKEEPVHFPELKCEKSDAYFVLRDGAS
CCCEEEHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCEECCCCCEEEEECCCC
GVFMSAHNFPKSRETRPVKIAELVQYRERLPEKLAYLADAPQKDPEENEAIVRFSRKEKK
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
QYVTSEKEGKATKWIVDFTNGKWVERKK
HHHCCCCCCCCEEEEEECCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]