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The map label for this gene is pntA

Identifier: 16273272

GI number: 16273272

Start: 1446885

End: 1448423

Strand: Direct

Name: pntA

Synonym: HI1362

Alternate gene names: 16273272

Gene position: 1446885-1448423 (Clockwise)

Preceding gene: 16273271

Following gene: 16273273

Centisome position: 79.06

GC content: 42.88

Gene sequence:

>1539_bases
ATGTTAATTGGTGTACCTAGAGAGCTGCTTGAGAATGAGAGCCGTGTGGCGGCAACGCCGAAAACGGTTCAGCAAATATT
GAAATTGGGCTTTGACGTGATCGTGGAACACGATGCAGGTTTTAAAGCTAGTTTTGAAGATCAAGCATTTTTAGAAGCGG
GCGCGAAGATCGGTACGTCGGCTGAAATTTGGCAATCGGATATTATCTTCAAAGTAAATGCACCGACAGATGAAGAAATT
GCTCAAATGAAGGAAGGGGCAGCTCTCGTGAGCTTTATTTGGCGCATGCAAAATCCTGAGTTAATGAAAAAACTCACCGC
GAAGAAAATTAATGTGTTAGCAATGGATGCGGTGCCTCGTATTTCTCGTGCGCAAGCATTGGATGCGCTTTCTTCTATGG
CGAATATTTCTGGTTATCGTGCTGTCATTGAAGCTGCGCATGAATTCGGTAGCTTCTTTACTGGGCAAATTACTGCAGCA
GGTAAAGTTCCACCAGCAAAAGTGTTGGTTATTGGTGCGGGTGTGGCTGGTTTGGCGGCAATTGGTGCAGCAAATAGCCT
TGGGGCGATTGTTCGTGCTTTTGACTCTCGTCCTGAAGTGAAAGAGCAAGTTCAGAGTATGGGCGCGAGCTTCTTAGAAA
TTGATTTCAAAGAAGAAGGTGGCAGTGGCGATGGTTATGCGAAAGTAATGTCGGAAGAATTTAACCGTCGTGCGATGGAG
CTTTACGCAGAACAAGCTAAAGAAGTCGATATTATTATTACTACGGCAGCCATTCCGGGTAAACCAGCTCCTCGTTTAAT
CACCAAAGAAATGGTGGATTCAATGAAACCAGGTTCGGTGATTGTGGATTTAGCGGCTGCAACTGGCGGTAACTGTGAAT
ATACGCAAGCAGGGAAAGTTGTTACAACTGAAAACCAAGTGAAAGTGATTGGTTATACAGATTTCCCAAGCCGTTTACCA
ACGCAATCTTCTCAACTTTATGGCACAAACTTAGTCAATTTATTAAAACTTTTGTGCAAAGAGAAAGATGGCAATATTAA
TATTGATTTTGAAGATGTGGTTCTTCGTGGTGTAACCGTTGTTCGTGATGGGGAAGAAATTCCACCAGCACAAATTCAAG
TGTCTGCACAACCAAAACAAGAAACCAAAGCTGCACCAGTGGCAGAGAAAAAAGAATCTAAACCAACAGATCCTCGTGTG
AAATACGGTGTGATGGCTGGTGTTGGCGTGTTATTCCTTTGGTTAGCTTCTGTGGCGCCCGCTGCATTTTTATCACATTT
CACCGTATTTGTTTTAGCTTGTGTGGTGGGTTATTACGTGGTTTGGAATGTTAGCCATGCACTTCACACACCATTAATGG
CAGTAACAAATGCAATTTCAGGCATTATTATTGTAGGTGCATTATTACAAATTCGTCAGCCAACAGGCAATCTATTTATC
GATGCGTTAGCTTTTGTGGCGATTTTGGTGGCAAGCATCAATATTTTTGGTGGTTTCCGTGTCACACAACGTATGTTAGC
AATGTTTAGAAAAGGTTAA

Upstream 100 bases:

>100_bases
TACATTTGCATCCGTTTGACTTTTTGTCATTTTAACGATGAATAGACGAAGGTTTGTTCATTAATCTCATCAACCCAATC
ATCAATCAGAGGGAAACAGT

Downstream 100 bases:

>100_bases
GGAGCGCACAATGTCTGAAGGTTTAGTACAGGCTGCGTACATTTTAGCAGCATTACTTTTCATTATGAGCTTGGCAGGAC
TTTCTAAACATGAAACTGCT

Product: NAD(P) transhydrogenase subunit alpha

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha; Pyridine nucleotide transhydrogenase subunit alpha

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MLIGVPRELLENESRVAATPKTVQQILKLGFDVIVEHDAGFKASFEDQAFLEAGAKIGTSAEIWQSDIIFKVNAPTDEEI
AQMKEGAALVSFIWRMQNPELMKKLTAKKINVLAMDAVPRISRAQALDALSSMANISGYRAVIEAAHEFGSFFTGQITAA
GKVPPAKVLVIGAGVAGLAAIGAANSLGAIVRAFDSRPEVKEQVQSMGASFLEIDFKEEGGSGDGYAKVMSEEFNRRAME
LYAEQAKEVDIIITTAAIPGKPAPRLITKEMVDSMKPGSVIVDLAAATGGNCEYTQAGKVVTTENQVKVIGYTDFPSRLP
TQSSQLYGTNLVNLLKLLCKEKDGNINIDFEDVVLRGVTVVRDGEEIPPAQIQVSAQPKQETKAAPVAEKKESKPTDPRV
KYGVMAGVGVLFLWLASVAPAAFLSHFTVFVLACVVGYYVVWNVSHALHTPLMAVTNAISGIIIVGALLQIRQPTGNLFI
DALAFVAILVASINIFGGFRVTQRMLAMFRKG

Sequences:

>Translated_512_residues
MLIGVPRELLENESRVAATPKTVQQILKLGFDVIVEHDAGFKASFEDQAFLEAGAKIGTSAEIWQSDIIFKVNAPTDEEI
AQMKEGAALVSFIWRMQNPELMKKLTAKKINVLAMDAVPRISRAQALDALSSMANISGYRAVIEAAHEFGSFFTGQITAA
GKVPPAKVLVIGAGVAGLAAIGAANSLGAIVRAFDSRPEVKEQVQSMGASFLEIDFKEEGGSGDGYAKVMSEEFNRRAME
LYAEQAKEVDIIITTAAIPGKPAPRLITKEMVDSMKPGSVIVDLAAATGGNCEYTQAGKVVTTENQVKVIGYTDFPSRLP
TQSSQLYGTNLVNLLKLLCKEKDGNINIDFEDVVLRGVTVVRDGEEIPPAQIQVSAQPKQETKAAPVAEKKESKPTDPRV
KYGVMAGVGVLFLWLASVAPAAFLSHFTVFVLACVVGYYVVWNVSHALHTPLMAVTNAISGIIIVGALLQIRQPTGNLFI
DALAFVAILVASINIFGGFRVTQRMLAMFRKG
>Mature_512_residues
MLIGVPRELLENESRVAATPKTVQQILKLGFDVIVEHDAGFKASFEDQAFLEAGAKIGTSAEIWQSDIIFKVNAPTDEEI
AQMKEGAALVSFIWRMQNPELMKKLTAKKINVLAMDAVPRISRAQALDALSSMANISGYRAVIEAAHEFGSFFTGQITAA
GKVPPAKVLVIGAGVAGLAAIGAANSLGAIVRAFDSRPEVKEQVQSMGASFLEIDFKEEGGSGDGYAKVMSEEFNRRAME
LYAEQAKEVDIIITTAAIPGKPAPRLITKEMVDSMKPGSVIVDLAAATGGNCEYTQAGKVVTTENQVKVIGYTDFPSRLP
TQSSQLYGTNLVNLLKLLCKEKDGNINIDFEDVVLRGVTVVRDGEEIPPAQIQVSAQPKQETKAAPVAEKKESKPTDPRV
KYGVMAGVGVLFLWLASVAPAAFLSHFTVFVLACVVGYYVVWNVSHALHTPLMAVTNAISGIIIVGALLQIRQPTGNLFI
DALAFVAILVASINIFGGFRVTQRMLAMFRKG

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family

Homologues:

Organism=Homo sapiens, GI122939155, Length=541, Percent_Identity=48.4288354898336, Blast_Score=456, Evalue=1e-128,
Organism=Homo sapiens, GI122939153, Length=541, Percent_Identity=48.4288354898336, Blast_Score=456, Evalue=1e-128,
Organism=Escherichia coli, GI1787887, Length=512, Percent_Identity=73.6328125, Blast_Score=743, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17550456, Length=521, Percent_Identity=48.7523992322457, Blast_Score=461, Evalue=1e-130,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PNTA_HAEIN (P43842)

Other databases:

- EMBL:   L42023
- PIR:   E64119
- RefSeq:   NP_439513.1
- ProteinModelPortal:   P43842
- SMR:   P43842
- GeneID:   950278
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1362
- NMPDR:   fig|71421.1.peg.1298
- TIGR:   HI_1362
- HOGENOM:   HBG289735
- OMA:   FKVNAPT
- ProtClustDB:   PRK09424
- BioCyc:   HINF71421:HI_1362-MONOMER
- BRENDA:   1.6.1.2
- GO:   GO:0005488
- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR016040
- InterPro:   IPR004571
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR00561

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N

EC number: =1.6.1.2

Molecular weight: Translated: 54973; Mature: 54973

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00836 ALADH_PNT_1; PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xdfb43e0)-; HASH(0xe740b5c)-; HASH(0xe5d4170)-; HASH(0xde397a4)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIGVPRELLENESRVAATPKTVQQILKLGFDVIVEHDAGFKASFEDQAFLEAGAKIGTS
CCCCCCHHHHCCCCCEECCHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHCCCCCCC
AEIWQSDIIFKVNAPTDEEIAQMKEGAALVSFIWRMQNPELMKKLTAKKINVLAMDAVPR
HHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEECCCCC
ISRAQALDALSSMANISGYRAVIEAAHEFGSFFTGQITAAGKVPPAKVLVIGAGVAGLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECEEEECCCCCCCEEEEEECCHHHHHH
IGAANSLGAIVRAFDSRPEVKEQVQSMGASFLEIDFKEEGGSGDGYAKVMSEEFNRRAME
HCCCHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHH
LYAEQAKEVDIIITTAAIPGKPAPRLITKEMVDSMKPGSVIVDLAAATGGNCEYTQAGKV
HHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCEECCCCE
VTTENQVKVIGYTDFPSRLPTQSSQLYGTNLVNLLKLLCKEKDGNINIDFEDVVLRGVTV
EEECCCEEEEEECCCCCCCCCCCCHHCCHHHHHHHHHHHCCCCCCEECCHHHHHHHCEEE
VRDGEEIPPAQIQVSAQPKQETKAAPVAEKKESKPTDPRVKYGVMAGVGVLFLWLASVAP
EECCCCCCCCEEEEECCCCHHHCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AAFLSHFTVFVLACVVGYYVVWNVSHALHTPLMAVTNAISGIIIVGALLQIRQPTGNLFI
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
DALAFVAILVASINIFGGFRVTQRMLAMFRKG
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCH
>Mature Secondary Structure
MLIGVPRELLENESRVAATPKTVQQILKLGFDVIVEHDAGFKASFEDQAFLEAGAKIGTS
CCCCCCHHHHCCCCCEECCHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHCCCCCCC
AEIWQSDIIFKVNAPTDEEIAQMKEGAALVSFIWRMQNPELMKKLTAKKINVLAMDAVPR
HHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEECCCCC
ISRAQALDALSSMANISGYRAVIEAAHEFGSFFTGQITAAGKVPPAKVLVIGAGVAGLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECEEEECCCCCCCEEEEEECCHHHHHH
IGAANSLGAIVRAFDSRPEVKEQVQSMGASFLEIDFKEEGGSGDGYAKVMSEEFNRRAME
HCCCHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHH
LYAEQAKEVDIIITTAAIPGKPAPRLITKEMVDSMKPGSVIVDLAAATGGNCEYTQAGKV
HHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCEECCCCE
VTTENQVKVIGYTDFPSRLPTQSSQLYGTNLVNLLKLLCKEKDGNINIDFEDVVLRGVTV
EEECCCEEEEEECCCCCCCCCCCCHHCCHHHHHHHHHHHCCCCCCEECCHHHHHHHCEEE
VRDGEEIPPAQIQVSAQPKQETKAAPVAEKKESKPTDPRVKYGVMAGVGVLFLWLASVAP
EECCCCCCCCEEEEECCCCHHHCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AAFLSHFTVFVLACVVGYYVVWNVSHALHTPLMAVTNAISGIIIVGALLQIRQPTGNLFI
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
DALAFVAILVASINIFGGFRVTQRMLAMFRKG
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800