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The map label for this gene is purF

Identifier: 16273127

GI number: 16273127

Start: 1273386

End: 1274903

Strand: Direct

Name: purF

Synonym: HI1207

Alternate gene names: 16273127

Gene position: 1273386-1274903 (Clockwise)

Preceding gene: 16273126

Following gene: 16273130

Centisome position: 69.58

GC content: 40.65

Gene sequence:

>1518_bases
ATGTGTGGTATTGTCGGTATTGTTAGCCAAAGTCCGGTTAATGAATCTATTTATGCAGCATTAACCCTTTTGCAACATCG
TGGACAAGATGCGGCAGGTATTGTAACGGTTGATGATGAAAACCGTTTTCGCTTACGTAAAGCTAATGGTTTAGTCAGTG
ATGTGTTTCATCAAGAACATATGCTACGATTGCAAGGAAATGCTGGATTGGGGCATGTTCGCTATCCTACAGCGGGGAGT
TCTAGCGTCTCAGAGGCACAGCCTTTTTATGTGAATTCTCCCTATGGTGTCACTTTAGTGCATAACGGCAACTTAACCAA
CTCAGTGGAGTTAAAAGAAAAGGTCTTTAAAACTGCTCGCCGCCATGTAAACACTAATTCAGATTCCGAACTTCTTCTTA
ATATTCTCGCCAATCACCTCGATCACATTCCTCAAGATCACCTCGATCCACAAGATATTTTCTATGCTGTTCGTAAAACT
CACAAGGATGTTCGCGGTGCTTATGCTTGTTTAGCGATGATTATTGGGCATGGCATGGTTGCGTTTCGTGATCCTTTTGG
CATTCGTCCTTTGGTGTTGGGTAAACGCGAGGAAAATGGCAAAACCGATTATATGTTTGCGTCTGAAACAGTGGCTTTAG
ATATTGTAGGTTTCGAATTTGTGCGTGATATTGCTGCAGGCGAAGCTGTTTATGTGACGTTTGATGGCGAGCTTTATTCA
CAACAATGTGCTGAAAGTGCGGTGTTAAATCCTTGTATTTTTGAGTATGTTTATTTTGCACGCCCAGATTCAACGATTGA
TGGCGTCTCTGTTTATGCTGCGCGTGTTCACATGGGCGAAAAACTTGGACAAAAAATTGCAAAAGAATGGGCTGATGAAA
TTGATAATATTGATGTGGTGATTCCCGTTCCTGAAACTTCAACAGATATTGCGTTACAAATAGCTCGTGTACTAGGGAAG
CCTTATCGCCAAGGATTTGTGAAAAATCGCTATGTTGGCCGTACCTTTATTATGCCAGGACAAGCACAGCGTATTAGTTC
GGTTCGTCGTAAATTGAATACCATTAAAGCTGAATTTAAAGATAAAAACGTGTTATTGGTAGACGATTCTATTGTTCGTG
GCACAACCTCTGAGCAAATTGTTGAAATGGCTCGCTCAGCTGGTGCGAAGAAAATTTACTTCGCCTCGGCTGCTCCAGAA
ATTCGTTATCCGAATGTATATGGTATTGATATGCCGAGTCGCGATGAACTGATCGCTTATGGACGAAATGTTGATGAAAT
TGCAGAATTAATTGGCGTAGATAAATTAATTTTCCAAGATCTGACCGCACTTACTGAATCTGTTCAATTGGAAAACCCAG
CCATTCAAGGTTTTGATTGTTCTGTTTTCACAGGCGAATATATTACAGGCGATATTAGCCCTGAATATTTAGAGAAAATC
GCAACTCAACGCAATGATAACGCAAAGAAGAAACGAGAAAAACAAGCATCCAATCTTGAAATTTACAATGAACAATAA

Upstream 100 bases:

>100_bases
TTAATTCCGCACTTTGGTTTTATTATTGAATGGTTCTTTCAACAACTTCAGGCGAGTTCAAGTTTTTTAACTCCAACGCT
TAATCAATAAGGAACACAAA

Downstream 100 bases:

>100_bases
ATGCTAGGCATTTAGAGGGATAAAAATCATCGTATTAAAAGTGCGGTGATTTTTTTATTTGTTTTTTAGGATGTCTTCTA
GATAGTTTTCGCAATCAGCA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MCGIVGIVSQSPVNESIYAALTLLQHRGQDAAGIVTVDDENRFRLRKANGLVSDVFHQEHMLRLQGNAGLGHVRYPTAGS
SSVSEAQPFYVNSPYGVTLVHNGNLTNSVELKEKVFKTARRHVNTNSDSELLLNILANHLDHIPQDHLDPQDIFYAVRKT
HKDVRGAYACLAMIIGHGMVAFRDPFGIRPLVLGKREENGKTDYMFASETVALDIVGFEFVRDIAAGEAVYVTFDGELYS
QQCAESAVLNPCIFEYVYFARPDSTIDGVSVYAARVHMGEKLGQKIAKEWADEIDNIDVVIPVPETSTDIALQIARVLGK
PYRQGFVKNRYVGRTFIMPGQAQRISSVRRKLNTIKAEFKDKNVLLVDDSIVRGTTSEQIVEMARSAGAKKIYFASAAPE
IRYPNVYGIDMPSRDELIAYGRNVDEIAELIGVDKLIFQDLTALTESVQLENPAIQGFDCSVFTGEYITGDISPEYLEKI
ATQRNDNAKKKREKQASNLEIYNEQ

Sequences:

>Translated_505_residues
MCGIVGIVSQSPVNESIYAALTLLQHRGQDAAGIVTVDDENRFRLRKANGLVSDVFHQEHMLRLQGNAGLGHVRYPTAGS
SSVSEAQPFYVNSPYGVTLVHNGNLTNSVELKEKVFKTARRHVNTNSDSELLLNILANHLDHIPQDHLDPQDIFYAVRKT
HKDVRGAYACLAMIIGHGMVAFRDPFGIRPLVLGKREENGKTDYMFASETVALDIVGFEFVRDIAAGEAVYVTFDGELYS
QQCAESAVLNPCIFEYVYFARPDSTIDGVSVYAARVHMGEKLGQKIAKEWADEIDNIDVVIPVPETSTDIALQIARVLGK
PYRQGFVKNRYVGRTFIMPGQAQRISSVRRKLNTIKAEFKDKNVLLVDDSIVRGTTSEQIVEMARSAGAKKIYFASAAPE
IRYPNVYGIDMPSRDELIAYGRNVDEIAELIGVDKLIFQDLTALTESVQLENPAIQGFDCSVFTGEYITGDISPEYLEKI
ATQRNDNAKKKREKQASNLEIYNEQ
>Mature_505_residues
MCGIVGIVSQSPVNESIYAALTLLQHRGQDAAGIVTVDDENRFRLRKANGLVSDVFHQEHMLRLQGNAGLGHVRYPTAGS
SSVSEAQPFYVNSPYGVTLVHNGNLTNSVELKEKVFKTARRHVNTNSDSELLLNILANHLDHIPQDHLDPQDIFYAVRKT
HKDVRGAYACLAMIIGHGMVAFRDPFGIRPLVLGKREENGKTDYMFASETVALDIVGFEFVRDIAAGEAVYVTFDGELYS
QQCAESAVLNPCIFEYVYFARPDSTIDGVSVYAARVHMGEKLGQKIAKEWADEIDNIDVVIPVPETSTDIALQIARVLGK
PYRQGFVKNRYVGRTFIMPGQAQRISSVRRKLNTIKAEFKDKNVLLVDDSIVRGTTSEQIVEMARSAGAKKIYFASAAPE
IRYPNVYGIDMPSRDELIAYGRNVDEIAELIGVDKLIFQDLTALTESVQLENPAIQGFDCSVFTGEYITGDISPEYLEKI
ATQRNDNAKKKREKQASNLEIYNEQ

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain

Homologues:

Organism=Homo sapiens, GI29570798, Length=477, Percent_Identity=35.2201257861635, Blast_Score=263, Evalue=2e-70,
Organism=Escherichia coli, GI1788651, Length=506, Percent_Identity=69.1699604743083, Blast_Score=732, Evalue=0.0,
Organism=Escherichia coli, GI1790167, Length=224, Percent_Identity=29.4642857142857, Blast_Score=70, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17554892, Length=483, Percent_Identity=33.3333333333333, Blast_Score=223, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6323958, Length=511, Percent_Identity=49.5107632093933, Blast_Score=490, Evalue=1e-139,
Organism=Drosophila melanogaster, GI24659598, Length=496, Percent_Identity=33.6693548387097, Blast_Score=261, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24659604, Length=475, Percent_Identity=34.3157894736842, Blast_Score=259, Evalue=4e-69,
Organism=Drosophila melanogaster, GI28573187, Length=499, Percent_Identity=33.4669338677355, Blast_Score=255, Evalue=4e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUR1_HAEIN (P43854)

Other databases:

- EMBL:   L42023
- PIR:   I64189
- RefSeq:   NP_439363.1
- ProteinModelPortal:   P43854
- SMR:   P43854
- MEROPS:   C44.001
- GeneID:   950171
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1207
- NMPDR:   fig|71421.1.peg.1154
- TIGR:   HI_1207
- HOGENOM:   HBG392416
- OMA:   SYCTACF
- ProtClustDB:   PRK09246
- BioCyc:   HINF71421:HI_1207-MONOMER
- BRENDA:   2.4.2.14
- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836
- PANTHER:   PTHR11907
- PIRSF:   PIRSF000485
- TIGRFAMs:   TIGR01134

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran

EC number: =2.4.2.14

Molecular weight: Translated: 56206; Mature: 56206

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS51278 GATASE_TYPE_2; PS00103 PUR_PYR_PR_TRANSFER; PS00443 GATASE_TYPE_II

Important sites: ACT_SITE 2-2

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVGIVSQSPVNESIYAALTLLQHRGQDAAGIVTVDDENRFRLRKANGLVSDVFHQEH
CCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEHHCCHHHHHHHHHH
MLRLQGNAGLGHVRYPTAGSSSVSEAQPFYVNSPYGVTLVHNGNLTNSVELKEKVFKTAR
EEEEECCCCCCEEECCCCCCCCCCCCCCEEEECCCCEEEEECCCCCCHHHHHHHHHHHHH
RHVNTNSDSELLLNILANHLDHIPQDHLDPQDIFYAVRKTHKDVRGAYACLAMIIGHGMV
HHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
AFRDPFGIRPLVLGKREENGKTDYMFASETVALDIVGFEFVRDIAAGEAVYVTFDGELYS
EECCCCCCCCEEECCCCCCCCCCEEEECCEEEEEEHHHHHHHHHHCCCEEEEEECCHHHH
QQCAESAVLNPCIFEYVYFARPDSTIDGVSVYAARVHMGEKLGQKIAKEWADEIDNIDVV
HHHHHHHHCCHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
IPVPETSTDIALQIARVLGKPYRQGFVKNRYVGRTFIMPGQAQRISSVRRKLNTIKAEFK
EECCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCC
DKNVLLVDDSIVRGTTSEQIVEMARSAGAKKIYFASAAPEIRYPNVYGIDMPSRDELIAY
CCCEEEECCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCCCHHCC
GRNVDEIAELIGVDKLIFQDLTALTESVQLENPAIQGFDCSVFTGEYITGDISPEYLEKI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCEEECCCCHHHHHHH
ATQRNDNAKKKREKQASNLEIYNEQ
HHCCCCCHHHHHHHHHCCCEEECCC
>Mature Secondary Structure
MCGIVGIVSQSPVNESIYAALTLLQHRGQDAAGIVTVDDENRFRLRKANGLVSDVFHQEH
CCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEHHCCHHHHHHHHHH
MLRLQGNAGLGHVRYPTAGSSSVSEAQPFYVNSPYGVTLVHNGNLTNSVELKEKVFKTAR
EEEEECCCCCCEEECCCCCCCCCCCCCCEEEECCCCEEEEECCCCCCHHHHHHHHHHHHH
RHVNTNSDSELLLNILANHLDHIPQDHLDPQDIFYAVRKTHKDVRGAYACLAMIIGHGMV
HHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
AFRDPFGIRPLVLGKREENGKTDYMFASETVALDIVGFEFVRDIAAGEAVYVTFDGELYS
EECCCCCCCCEEECCCCCCCCCCEEEECCEEEEEEHHHHHHHHHHCCCEEEEEECCHHHH
QQCAESAVLNPCIFEYVYFARPDSTIDGVSVYAARVHMGEKLGQKIAKEWADEIDNIDVV
HHHHHHHHCCHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
IPVPETSTDIALQIARVLGKPYRQGFVKNRYVGRTFIMPGQAQRISSVRRKLNTIKAEFK
EECCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCC
DKNVLLVDDSIVRGTTSEQIVEMARSAGAKKIYFASAAPEIRYPNVYGIDMPSRDELIAY
CCCEEEECCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCCCHHCC
GRNVDEIAELIGVDKLIFQDLTALTESVQLENPAIQGFDCSVFTGEYITGDISPEYLEKI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCEEECCCCHHHHHHH
ATQRNDNAKKKREKQASNLEIYNEQ
HHCCCCCHHHHHHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800