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The map label for this gene is mdh [H]

Identifier: 16273130

GI number: 16273130

Start: 1276510

End: 1277445

Strand: Direct

Name: mdh [H]

Synonym: HI1210

Alternate gene names: 16273130

Gene position: 1276510-1277445 (Clockwise)

Preceding gene: 16273127

Following gene: 16273132

Centisome position: 69.75

GC content: 40.81

Gene sequence:

>936_bases
ATGAAAGTTGCTGTATTAGGTGCCGCAGGTGGTATTGGTCAAGCATTAGCGTTATTACTAAAACTTCAGTTGCCAGCAGG
TACCGACTTATCATTGTATGATATTGCCCCTGTTACCCCAGGTGTTGCAGTGGATGTGAGCCATATTCCAACGGCAGTGA
ATGTGAAAGGTTTTTCTGGTGAAGATCCAACTCCAGCACTTGAAGGTGCGGATGTTGTATTAATTTCTGCTGGTGTTGCA
CGTAAACCTGGTATGGATCGTTCAGATTTATTCAATATTAATGCAGGTATCGTGCGTGGTTTAATTGAAAAAGTCGCGGT
TACTTGTCCGAAAGCATGCGTTGGTATCATCACTAACCCAGTAAATACTACCGTTGCGATTGCGGCTGAAGTGCTGAAAA
AAGCAGGCGTTTACGACAAACGTAAATTATTTGGTGTGACAACTTTAGACGTGTTGCGTTCTGAAACCTTTGTGGCTGAA
TTAAAAGGTTTAAATGTTTCTCGTACAAGCGTTCCTGTTATTGGTGGTCACTCTGGTGTGACTATTCTTCCATTACTTTC
TCAAGTTCAATATGCAAAATGGAATGAAGATGAAATCGAACCATTAACAAAACGTATCCAAAATGCAGGTACAGAAGTGC
TCAATGCAAAAGCGGGTGGCGGTTCTGCAACCCTTTCAATGGCGCAAGCTGCAGCACGTTTTGCGCGTTCTTTAGTGAAA
GGATTAAGTGGCGAGACAGTGGTTGAATGTACTTATGTTGAAGGTGATGGAAAATATGCTCGTTTCTTCTCTCAGCCAGT
TCGTTTAGGTAAAGAAGGTGTAGAAGAAATTTTACCAATTGGTCCATTAAGCAATTTTGAACAACAAGCCTTAGAAAATA
TGTTGCCAACTTTACGTGCAGATATTGAATTAGGCGAAAAATTTATTAATGGTTAA

Upstream 100 bases:

>100_bases
TGTGAACTAGATCATAGAATGATATTTCTCTATTTGTAATTGTAAGATGAACATTTTAAAATCAGTCTGTTTTAAATTAA
CTAACACATAAGGAGTATTT

Downstream 100 bases:

>100_bases
TCATTAAAAAATAAGGGGGAGTTAGCTCTCTTTTTATGTTTCGATAAAAAAGCCACCAAATGGTGGCTTTTGTTTATATC
TTTATTATTTCTGACGCATT

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MKVAVLGAAGGIGQALALLLKLQLPAGTDLSLYDIAPVTPGVAVDVSHIPTAVNVKGFSGEDPTPALEGADVVLISAGVA
RKPGMDRSDLFNINAGIVRGLIEKVAVTCPKACVGIITNPVNTTVAIAAEVLKKAGVYDKRKLFGVTTLDVLRSETFVAE
LKGLNVSRTSVPVIGGHSGVTILPLLSQVQYAKWNEDEIEPLTKRIQNAGTEVLNAKAGGGSATLSMAQAAARFARSLVK
GLSGETVVECTYVEGDGKYARFFSQPVRLGKEGVEEILPIGPLSNFEQQALENMLPTLRADIELGEKFING

Sequences:

>Translated_311_residues
MKVAVLGAAGGIGQALALLLKLQLPAGTDLSLYDIAPVTPGVAVDVSHIPTAVNVKGFSGEDPTPALEGADVVLISAGVA
RKPGMDRSDLFNINAGIVRGLIEKVAVTCPKACVGIITNPVNTTVAIAAEVLKKAGVYDKRKLFGVTTLDVLRSETFVAE
LKGLNVSRTSVPVIGGHSGVTILPLLSQVQYAKWNEDEIEPLTKRIQNAGTEVLNAKAGGGSATLSMAQAAARFARSLVK
GLSGETVVECTYVEGDGKYARFFSQPVRLGKEGVEEILPIGPLSNFEQQALENMLPTLRADIELGEKFING
>Mature_311_residues
MKVAVLGAAGGIGQALALLLKLQLPAGTDLSLYDIAPVTPGVAVDVSHIPTAVNVKGFSGEDPTPALEGADVVLISAGVA
RKPGMDRSDLFNINAGIVRGLIEKVAVTCPKACVGIITNPVNTTVAIAAEVLKKAGVYDKRKLFGVTTLDVLRSETFVAE
LKGLNVSRTSVPVIGGHSGVTILPLLSQVQYAKWNEDEIEPLTKRIQNAGTEVLNAKAGGGSATLSMAQAAARFARSLVK
GLSGETVVECTYVEGDGKYARFFSQPVRLGKEGVEEILPIGPLSNFEQQALENMLPTLRADIELGEKFING

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 1 family [H]

Homologues:

Organism=Homo sapiens, GI21735621, Length=311, Percent_Identity=55.6270096463022, Blast_Score=320, Evalue=2e-87,
Organism=Escherichia coli, GI1789632, Length=311, Percent_Identity=79.0996784565916, Blast_Score=486, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI17554310, Length=313, Percent_Identity=58.14696485623, Blast_Score=338, Evalue=3e-93,
Organism=Saccharomyces cerevisiae, GI6322765, Length=315, Percent_Identity=50.1587301587302, Blast_Score=273, Evalue=2e-74,
Organism=Saccharomyces cerevisiae, GI6320125, Length=338, Percent_Identity=39.6449704142012, Blast_Score=186, Evalue=4e-48,
Organism=Saccharomyces cerevisiae, GI6324446, Length=278, Percent_Identity=43.1654676258993, Blast_Score=181, Evalue=9e-47,
Organism=Drosophila melanogaster, GI24647881, Length=312, Percent_Identity=58.6538461538462, Blast_Score=334, Evalue=5e-92,
Organism=Drosophila melanogaster, GI24663599, Length=316, Percent_Identity=49.6835443037975, Blast_Score=274, Evalue=6e-74,
Organism=Drosophila melanogaster, GI24663595, Length=298, Percent_Identity=44.6308724832215, Blast_Score=250, Evalue=8e-67,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR001252
- InterPro:   IPR010097
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 32543; Mature: 32543

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00068 MDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVAVLGAAGGIGQALALLLKLQLPAGTDLSLYDIAPVTPGVAVDVSHIPTAVNVKGFSG
CEEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEEHHHCCCEEEECCCCC
EDPTPALEGADVVLISAGVARKPGMDRSDLFNINAGIVRGLIEKVAVTCPKACVGIITNP
CCCCCCCCCCCEEEEECCCCCCCCCCHHHEEECHHHHHHHHHHHHHHHCCHHHHHHHCCC
VNTTVAIAAEVLKKAGVYDKRKLFGVTTLDVLRSETFVAELKGLNVSRTSVPVIGGHSGV
CHHHHHHHHHHHHHCCCCCHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCC
TILPLLSQVQYAKWNEDEIEPLTKRIQNAGTEVLNAKAGGGSATLSMAQAAARFARSLVK
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHEECCCCCCHHHHHHHHHHHHHHHHHHH
GLSGETVVECTYVEGDGKYARFFSQPVRLGKEGVEEILPIGPLSNFEQQALENMLPTLRA
CCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
DIELGEKFING
HHHHHHHHHCC
>Mature Secondary Structure
MKVAVLGAAGGIGQALALLLKLQLPAGTDLSLYDIAPVTPGVAVDVSHIPTAVNVKGFSG
CEEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEEHHHCCCEEEECCCCC
EDPTPALEGADVVLISAGVARKPGMDRSDLFNINAGIVRGLIEKVAVTCPKACVGIITNP
CCCCCCCCCCCEEEEECCCCCCCCCCHHHEEECHHHHHHHHHHHHHHHCCHHHHHHHCCC
VNTTVAIAAEVLKKAGVYDKRKLFGVTTLDVLRSETFVAELKGLNVSRTSVPVIGGHSGV
CHHHHHHHHHHHHHCCCCCHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCC
TILPLLSQVQYAKWNEDEIEPLTKRIQNAGTEVLNAKAGGGSATLSMAQAAARFARSLVK
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHEECCCCCCHHHHHHHHHHHHHHHHHHH
GLSGETVVECTYVEGDGKYARFFSQPVRLGKEGVEEILPIGPLSNFEQQALENMLPTLRA
CCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
DIELGEKFING
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA