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The map label for this gene is glpF

Identifier: 16272632

GI number: 16272632

Start: 734270

End: 735064

Strand: Direct

Name: glpF

Synonym: HI0690

Alternate gene names: 16272632

Gene position: 734270-735064 (Clockwise)

Preceding gene: 16272631

Following gene: 16272633

Centisome position: 40.12

GC content: 43.52

Gene sequence:

>795_bases
ATGGATAAATCATTAAAAGCGAACTGTATTGGCGAGTTTTTAGGTACAGCCTTATTGATTTTCTTTGGTGTGGGCTGCGT
TGCAGCACTAAAAGTAGCAGGCGCTAGTTTTGGCTTGTGGGAAATCAGCATTATGTGGGGGATGGGCGTTGCACTTGCAG
TATATGCAACAGCGGGTTTATCTGGTGCACATCTAAACCCTGCAGTAACCATTGCCCTTTGGAAATTTGCTTGCTTTGAT
GGCAAAAAAGTAATTCCTTACATCATTTCACAAATGCTCGGCGCATTCTTTGCTGCCGCATTAGTTTATGCCTTATACCG
CAATGTTTTTATCGATTATGAAACAGTCCACAATATTGTTCGTGGTACACAAGAAAGTTTAAGCCTTGCGGGTACATTCT
CCACTTATCCTCATCCAAGTTTAAGCATTGGTGGCGCCTTTGCTGTGGAATTCGTGATTACTGCAATTCTTATGGCATTA
ATTATGGCATTAACTGACGATGGAAACGGAGTACCTCGTGGTCCGCTTGCCCCATTATTAATCGGTATCTTGATAGCCGT
TATTGGTGGTGCTATGGGACCATTAACTGGTTTTGCCATGAATCCAGCACGTGACTTCGGACCAAAATTCTTTGCTTATC
TCGCTGGCTGGGGCGAACTCGCTTTAACTGGCGGACGCGAAATCCCTTATTTTATCGTTCCAATGGTTGCACCTGTTTTA
GGTGCATTAGCTGGTGCATGGTTATACAAAAAAGCAATTGGCGGCAATTTACCTTGTAATTGCGGCTGCGAATAA

Upstream 100 bases:

>100_bases
AGGCTATTTCAAAACACTCCGTATTCTTTTTTAATAAATTCTCTTCCCTTTACTTAGGGAAAACACTCTTCATTTCAACC
GCACTTCTAAGGAGTGATCT

Downstream 100 bases:

>100_bases
TTATTTGAAGGAGTAATACTATGACAGACAAAAAATACATCATTGCATTAGACCAAGGCACAACAAGCTCTCGCGCAGTA
TTATTAGATCATAATGCGAA

Product: glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MDKSLKANCIGEFLGTALLIFFGVGCVAALKVAGASFGLWEISIMWGMGVALAVYATAGLSGAHLNPAVTIALWKFACFD
GKKVIPYIISQMLGAFFAAALVYALYRNVFIDYETVHNIVRGTQESLSLAGTFSTYPHPSLSIGGAFAVEFVITAILMAL
IMALTDDGNGVPRGPLAPLLIGILIAVIGGAMGPLTGFAMNPARDFGPKFFAYLAGWGELALTGGREIPYFIVPMVAPVL
GALAGAWLYKKAIGGNLPCNCGCE

Sequences:

>Translated_264_residues
MDKSLKANCIGEFLGTALLIFFGVGCVAALKVAGASFGLWEISIMWGMGVALAVYATAGLSGAHLNPAVTIALWKFACFD
GKKVIPYIISQMLGAFFAAALVYALYRNVFIDYETVHNIVRGTQESLSLAGTFSTYPHPSLSIGGAFAVEFVITAILMAL
IMALTDDGNGVPRGPLAPLLIGILIAVIGGAMGPLTGFAMNPARDFGPKFFAYLAGWGELALTGGREIPYFIVPMVAPVL
GALAGAWLYKKAIGGNLPCNCGCE
>Mature_264_residues
MDKSLKANCIGEFLGTALLIFFGVGCVAALKVAGASFGLWEISIMWGMGVALAVYATAGLSGAHLNPAVTIALWKFACFD
GKKVIPYIISQMLGAFFAAALVYALYRNVFIDYETVHNIVRGTQESLSLAGTFSTYPHPSLSIGGAFAVEFVITAILMAL
IMALTDDGNGVPRGPLAPLLIGILIAVIGGAMGPLTGFAMNPARDFGPKFFAYLAGWGELALTGGREIPYFIVPMVAPVL
GALAGAWLYKKAIGGNLPCNCGCE

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family

Homologues:

Organism=Homo sapiens, GI157266307, Length=251, Percent_Identity=39.0438247011952, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI4826645, Length=256, Percent_Identity=38.28125, Blast_Score=151, Evalue=5e-37,
Organism=Homo sapiens, GI22538420, Length=256, Percent_Identity=35.546875, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI4502187, Length=261, Percent_Identity=32.5670498084291, Blast_Score=135, Evalue=5e-32,
Organism=Homo sapiens, GI310133356, Length=207, Percent_Identity=29.951690821256, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI310114181, Length=207, Percent_Identity=29.951690821256, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI45446752, Length=205, Percent_Identity=30.7317073170732, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI86792455, Length=256, Percent_Identity=31.25, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI4502179, Length=241, Percent_Identity=31.1203319502075, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI6912506, Length=198, Percent_Identity=30.8080808080808, Blast_Score=68, Evalue=7e-12,
Organism=Escherichia coli, GI1790362, Length=258, Percent_Identity=73.6434108527132, Blast_Score=394, Evalue=1e-111,
Organism=Escherichia coli, GI1787101, Length=213, Percent_Identity=31.924882629108, Blast_Score=72, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71994009, Length=260, Percent_Identity=33.4615384615385, Blast_Score=147, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI71992966, Length=253, Percent_Identity=35.9683794466403, Blast_Score=137, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI32564052, Length=258, Percent_Identity=34.1085271317829, Blast_Score=136, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17533613, Length=258, Percent_Identity=34.1085271317829, Blast_Score=136, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17531431, Length=262, Percent_Identity=33.206106870229, Blast_Score=129, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17531429, Length=262, Percent_Identity=33.206106870229, Blast_Score=129, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17544068, Length=251, Percent_Identity=35.0597609561753, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71992961, Length=253, Percent_Identity=32.8063241106719, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17558372, Length=251, Percent_Identity=26.6932270916335, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6321054, Length=212, Percent_Identity=39.622641509434, Blast_Score=139, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6322985, Length=233, Percent_Identity=35.1931330472103, Blast_Score=103, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24762344, Length=247, Percent_Identity=28.7449392712551, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24762346, Length=247, Percent_Identity=28.7449392712551, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24762348, Length=247, Percent_Identity=28.7449392712551, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI20130305, Length=247, Percent_Identity=28.7449392712551, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI45550503, Length=248, Percent_Identity=26.2096774193548, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24652747, Length=207, Percent_Identity=32.3671497584541, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI45551084, Length=207, Percent_Identity=32.3671497584541, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24762342, Length=259, Percent_Identity=25.8687258687259, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLPF_HAEIN (P44826)

Other databases:

- EMBL:   L42023
- PIR:   H64086
- RefSeq:   NP_438850.1
- ProteinModelPortal:   P44826
- SMR:   P44826
- GeneID:   949956
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0690
- NMPDR:   fig|71421.1.peg.660
- TIGR:   HI_0690
- HOGENOM:   HBG705794
- OMA:   TIALWKF
- ProtClustDB:   CLSK2392968
- BioCyc:   HINF71421:HI_0690-MONOMER
- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357
- PANTHER:   PTHR19139
- PRINTS:   PR00783
- TIGRFAMs:   TIGR00861

Pfam domain/function: PF00230 MIP

EC number: NA

Molecular weight: Translated: 27610; Mature: 27610

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: PS00221 MIP; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xcc1ea9c)-; HASH(0xd4b0c30)-; HASH(0xcc8741c)-; HASH(0xd2aee64)-; HASH(0xbf079ac)-; HASH(0xc5beb60)-;

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKSLKANCIGEFLGTALLIFFGVGCVAALKVAGASFGLWEISIMWGMGVALAVYATAGL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCC
SGAHLNPAVTIALWKFACFDGKKVIPYIISQMLGAFFAAALVYALYRNVFIDYETVHNIV
CCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
RGTQESLSLAGTFSTYPHPSLSIGGAFAVEFVITAILMALIMALTDDGNGVPRGPLAPLL
HCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
IGILIAVIGGAMGPLTGFAMNPARDFGPKFFAYLAGWGELALTGGREIPYFIVPMVAPVL
HHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHH
GALAGAWLYKKAIGGNLPCNCGCE
HHHHHHHHHHHHHCCCCCCCCCCH
>Mature Secondary Structure
MDKSLKANCIGEFLGTALLIFFGVGCVAALKVAGASFGLWEISIMWGMGVALAVYATAGL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCC
SGAHLNPAVTIALWKFACFDGKKVIPYIISQMLGAFFAAALVYALYRNVFIDYETVHNIV
CCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
RGTQESLSLAGTFSTYPHPSLSIGGAFAVEFVITAILMALIMALTDDGNGVPRGPLAPLL
HCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
IGILIAVIGGAMGPLTGFAMNPARDFGPKFFAYLAGWGELALTGGREIPYFIVPMVAPVL
HHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHH
GALAGAWLYKKAIGGNLPCNCGCE
HHHHHHHHHHHHHCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800