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The map label for this gene is menF

Identifier: 16272240

GI number: 16272240

Start: 318376

End: 319668

Strand: Reverse

Name: menF

Synonym: HI0285

Alternate gene names: 16272240

Gene position: 319668-318376 (Counterclockwise)

Preceding gene: 16272243

Following gene: 16272239

Centisome position: 17.47

GC content: 38.9

Gene sequence:

>1293_bases
GTGTATTTAAAACAAAATCTAATGAGTTATTTAGCGCAGGCAATAGGCGAGTTAAAATCGCAAATTCACGCATATTTACA
GCAATCAACGAATGAACTTGTCCGTTTTCAGGTAAAACTGGACAAGGTCGATTTGTTGGCGTGGCTGAAAGGTCAATCTG
CGTATCCTCAATTTTATTTACATTTTCGTGATGAAGAAAAAGCATTGGCTGCGCTTGGGGCAGTGCAGTCATTTTCACAG
TTAAATTTAGCGCAAGAGTTTATTGAAGAAAGTGGTTTTCCACTGGTCGGCGGTTTGCAATTTCAAGGCACTGCACAATT
TGTATTACCTAAAATGCTTGTTGAACAGGATAATAAAGGCACGTTGGTATCCTTTTTTGTGAAAAATGAGCAAAGTGCGA
ATGATACTTTAGCCCACCTCAAAACTTTTGAAAATCTCACCGCACTTTCTGCTTTGCCAAAACAAATTCCTTTGCATACA
GAGCTTCGAGCCAATGAAAGAACTTGGTGTGATTGGGTAAATCAGGCTTTGGTAGAAATTAAAAGTGGAGAGCTGACAAA
AATTGTGTTAGCCAATGAAACCACTTTTCATTTAAAGCAAGCGATTAATGCGTACGATTTTTTAGCAGAAAGCGAAAAAC
AAAATCAAGGTTGTTATCATTTTTTATGGGCTGAAAATTCTCATTCGGTTTTTGTTGGTTCTACGCCAGAACGCCTATTT
GCTCGTGAGTATAATTTGTTGCTAACCGAAGCTTTGGCGGGGACAGCATCGGTTTCTGAAAGTGAGGAAGAAACGCAATC
TCAAGCCAATTGGTTGTTAAATGATGAGAAAAATTTAAAAGAAAATTGGTTGGTTGTAGAAGATATTTCGCAGAATTTAC
GTAAGCAAGTAGAAAGTTTTGATGTAAGCAATGTGGAATTGAAACCGTTACGTAAAGTACAACATTTGATTCGTAAAATT
CGTGCAAATTTGACCGCACATTATGCAGATGTAAATATATTAAAAGCGATTCATCCTACAGCTGCAGTATCTGGTTTGCC
ACAGCAACAAGCCAAAATGATTTTGTCAGAAATCGAAACCTTTGATCGAGGCTGGTATGCGGGAACATTGGGCGTGATGA
GCGATGTATGTTCAGAGTTTTGTGTGGCGATTCGTTCTGCTTTTATTGAAGGTCATCGTATTCGAGTATTTGCTGGCGCG
GGCATTGTGGCAGGCTCGCAGCCATTGGAAGAATGGAAAGAAATTGAACGTAAAGCAGCAGGGTTAATTTCCTTGTTTGC
AGAAGAAAAATGA

Upstream 100 bases:

>100_bases
AAAAGTGCGGTTAATTTACGCTGAATTTTCAATATTGAAAAGATTTTGTAAAAAAATTTTTTGTCATTAACAATCTGCGG
TAGAATATGGCTAATTTTTT

Downstream 100 bases:

>100_bases
TAACGGAGAAAAAAAATGTCGGTAAGCGTGTTTAATCGTTGTTGGTCGAAAGTGATTTTAGAAACCTTGGTGCGCCAAGG
CGTTTCTCACCTTTGTATCG

Product: menaquinone-specific isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MYLKQNLMSYLAQAIGELKSQIHAYLQQSTNELVRFQVKLDKVDLLAWLKGQSAYPQFYLHFRDEEKALAALGAVQSFSQ
LNLAQEFIEESGFPLVGGLQFQGTAQFVLPKMLVEQDNKGTLVSFFVKNEQSANDTLAHLKTFENLTALSALPKQIPLHT
ELRANERTWCDWVNQALVEIKSGELTKIVLANETTFHLKQAINAYDFLAESEKQNQGCYHFLWAENSHSVFVGSTPERLF
AREYNLLLTEALAGTASVSESEEETQSQANWLLNDEKNLKENWLVVEDISQNLRKQVESFDVSNVELKPLRKVQHLIRKI
RANLTAHYADVNILKAIHPTAAVSGLPQQQAKMILSEIETFDRGWYAGTLGVMSDVCSEFCVAIRSAFIEGHRIRVFAGA
GIVAGSQPLEEWKEIERKAAGLISLFAEEK

Sequences:

>Translated_430_residues
MYLKQNLMSYLAQAIGELKSQIHAYLQQSTNELVRFQVKLDKVDLLAWLKGQSAYPQFYLHFRDEEKALAALGAVQSFSQ
LNLAQEFIEESGFPLVGGLQFQGTAQFVLPKMLVEQDNKGTLVSFFVKNEQSANDTLAHLKTFENLTALSALPKQIPLHT
ELRANERTWCDWVNQALVEIKSGELTKIVLANETTFHLKQAINAYDFLAESEKQNQGCYHFLWAENSHSVFVGSTPERLF
AREYNLLLTEALAGTASVSESEEETQSQANWLLNDEKNLKENWLVVEDISQNLRKQVESFDVSNVELKPLRKVQHLIRKI
RANLTAHYADVNILKAIHPTAAVSGLPQQQAKMILSEIETFDRGWYAGTLGVMSDVCSEFCVAIRSAFIEGHRIRVFAGA
GIVAGSQPLEEWKEIERKAAGLISLFAEEK
>Mature_430_residues
MYLKQNLMSYLAQAIGELKSQIHAYLQQSTNELVRFQVKLDKVDLLAWLKGQSAYPQFYLHFRDEEKALAALGAVQSFSQ
LNLAQEFIEESGFPLVGGLQFQGTAQFVLPKMLVEQDNKGTLVSFFVKNEQSANDTLAHLKTFENLTALSALPKQIPLHT
ELRANERTWCDWVNQALVEIKSGELTKIVLANETTFHLKQAINAYDFLAESEKQNQGCYHFLWAENSHSVFVGSTPERLF
AREYNLLLTEALAGTASVSESEEETQSQANWLLNDEKNLKENWLVVEDISQNLRKQVESFDVSNVELKPLRKVQHLIRKI
RANLTAHYADVNILKAIHPTAAVSGLPQQQAKMILSEIETFDRGWYAGTLGVMSDVCSEFCVAIRSAFIEGHRIRVFAGA
GIVAGSQPLEEWKEIERKAAGLISLFAEEK

Specific function: Menaquinone biosynthesis. [C]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family

Homologues:

Organism=Escherichia coli, GI87082077, Length=409, Percent_Identity=38.3863080684597, Blast_Score=251, Evalue=8e-68,
Organism=Escherichia coli, GI1786809, Length=262, Percent_Identity=29.0076335877863, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787518, Length=336, Percent_Identity=23.8095238095238, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788114, Length=245, Percent_Identity=25.7142857142857, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320935, Length=249, Percent_Identity=26.1044176706827, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MENF_HAEIN (P44613)

Other databases:

- EMBL:   L42023
- PIR:   E64059
- RefSeq:   NP_438452.1
- ProteinModelPortal:   P44613
- SMR:   P44613
- GeneID:   949812
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0285
- NMPDR:   fig|71421.1.peg.267
- TIGR:   HI_0285
- HOGENOM:   HBG405459
- OMA:   WYAGTLG
- ProtClustDB:   CLSK869946
- BioCyc:   HINF71421:HI_0285-MONOMER
- BRENDA:   5.4.4.2
- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561
- Gene3D:   G3DSA:3.60.120.10
- PANTHER:   PTHR11236:SF3
- PANTHER:   PTHR11236
- TIGRFAMs:   TIGR00543

Pfam domain/function: PF00425 Chorismate_bind; SSF56322 TRPE_1_chor_bd

EC number: =5.4.4.2

Molecular weight: Translated: 48467; Mature: 48467

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLKQNLMSYLAQAIGELKSQIHAYLQQSTNELVRFQVKLDKVDLLAWLKGQSAYPQFYL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECHHHHHHHHCCCCCCCEEEE
HFRDEEKALAALGAVQSFSQLNLAQEFIEESGFPLVGGLQFQGTAQFVLPKMLVEQDNKG
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECEEECCCHHHHHHHHHHCCCCCC
TLVSFFVKNEQSANDTLAHLKTFENLTALSALPKQIPLHTELRANERTWCDWVNQALVEI
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHC
KSGELTKIVLANETTFHLKQAINAYDFLAESEKQNQGCYHFLWAENSHSVFVGSTPERLF
CCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEECCCHHHHH
AREYNLLLTEALAGTASVSESEEETQSQANWLLNDEKNLKENWLVVEDISQNLRKQVESF
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCHHCCEEHHHHHHHHHHHHHHC
DVSNVELKPLRKVQHLIRKIRANLTAHYADVNILKAIHPTAAVSGLPQQQAKMILSEIET
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCHHHHHCCCCHHHHHHHHHHHHH
FDRGWYAGTLGVMSDVCSEFCVAIRSAFIEGHRIRVFAGAGIVAGSQPLEEWKEIERKAA
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEECCCCHHHHHHHHHHHHH
GLISLFAEEK
HHHHHHHCCC
>Mature Secondary Structure
MYLKQNLMSYLAQAIGELKSQIHAYLQQSTNELVRFQVKLDKVDLLAWLKGQSAYPQFYL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECHHHHHHHHCCCCCCCEEEE
HFRDEEKALAALGAVQSFSQLNLAQEFIEESGFPLVGGLQFQGTAQFVLPKMLVEQDNKG
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECEEECCCHHHHHHHHHHCCCCCC
TLVSFFVKNEQSANDTLAHLKTFENLTALSALPKQIPLHTELRANERTWCDWVNQALVEI
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHC
KSGELTKIVLANETTFHLKQAINAYDFLAESEKQNQGCYHFLWAENSHSVFVGSTPERLF
CCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEECCCHHHHH
AREYNLLLTEALAGTASVSESEEETQSQANWLLNDEKNLKENWLVVEDISQNLRKQVESF
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCHHCCEEHHHHHHHHHHHHHHC
DVSNVELKPLRKVQHLIRKIRANLTAHYADVNILKAIHPTAAVSGLPQQQAKMILSEIET
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCHHHHHCCCCHHHHHHHHHHHHH
FDRGWYAGTLGVMSDVCSEFCVAIRSAFIEGHRIRVFAGAGIVAGSQPLEEWKEIERKAA
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEECCCCHHHHHHHHHHHHH
GLISLFAEEK
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800