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The map label for this gene is menD [H]

Identifier: 16272239

GI number: 16272239

Start: 316654

End: 318360

Strand: Reverse

Name: menD [H]

Synonym: HI0283

Alternate gene names: 16272239

Gene position: 318360-316654 (Counterclockwise)

Preceding gene: 16272240

Following gene: 16272238

Centisome position: 17.4

GC content: 40.95

Gene sequence:

>1707_bases
ATGTCGGTAAGCGTGTTTAATCGTTGTTGGTCGAAAGTGATTTTAGAAACCTTGGTGCGCCAAGGCGTTTCTCACCTTTG
TATCGCGCCAGGTTCGCGTTCGACACCTTTAACTCTAGAAGCCGTACGTTTGCAAAATGCGGGCGCAGTCACTTGTCATA
CGCATTTTGATGAACGCGGTTTAGGTTTTTTTGCTTTGGGTATTGCTAAGGCAACTCAATCGCCTGTTGCAATTATTGTT
ACATCAGGTACTGCAACAGCAAACCTTTATCCTGCCATTATTGAAGCACGCCAAACTGGTGTAAATTTATTTGTTTTAAC
TGCTGATCGTCCACCAGAACTTTGGGAGTGCGGTGCAAATCAGGCCATTTTGCAACAAAATATGTTTGGTCAGTATCCAG
TTGCAAATGTTAATTTACCTAAACCGAATGCTGATTATTCTGCGCAGTGGTTAATTTCTCTACTTGAACAGGCAGTTTTC
CAACAAAAACAACAAGGTGGCGTTGTTCATATTAATGTCCCATTTGCCGAGCCACTTTATGATGCAACTGATGAGGAAGT
AAATTCCCATAGTTGGCTACAGCCGTTACAACGCTGGTTAATTCAAAACAAGTCTTGGATAAATGTAGAAGCGCAACAAA
ACGAAGTATTAATGCATGAAAATTGGGATCATTGGCGTACCAAACGTGGTGTCGTTGTGGTTGGTCAATTACCTGCAGAA
CAAGCGATGGGCATTAATTCTTGGGCAAGTGCTATGGGCTGGGTGTTACTGACGGATATTCAATCTGGTGTTGTTCCAAC
GACGCCTTATGAAGATATTTGGCTGGCAAACCAAACTGTTCGTGAAAAACTTCTACAAGCTGATATTGTGATTCAATTTG
GCGCGCGTTTTATTAGTAAACGCATTAATCAATTCTTGCAAGCGTTTAAAGGGGAATTTTGGCTAGTTGAACAAAGCGGT
AAAGCATTAGATCCTTACCATCATTCATTGACAAGATTTAATGCTAAAGTACATCATTGGTTGCGTGCACATCCACCTTT
ACGCCAAAAGCCTTGGTTGCTTGAGCCGTTAGCTTTATCTAAGTTCTGTGCGACCTTTATTGAACAGCAAGTAGGCGGAA
ATTTAACGGAAGCCTCGCTCGCATTACGTTTACCAACACTTTTACCTTATAACGGTGTTTTATTTTTAGGTAATAGTTTA
CTTGTTCGTCTAGTTGATGCGCTAACGCAATTACCAGAAAGTTATCCCGTTTATACCAATCGTGGTGCGAGTGGAATTGA
TGGTTTGTTGGCTACTGCTGCTGGAATAGGTATTGGTTCAAATAAACCTGTTGTTGCGGTGATTGGCGATACGTCCACTT
TATATGATTTGAATTCTTTCGCATTATTCAAAAACGTTACGCAACCAACGCTAATCTTTGTGATCAATAATAATGGTGGC
GCGATTTTTGATATGTTACCTGTGGATGAACAAGTCAAAGATCAGTTCTATCGATTACCGCATAATGGCGATTTTTCTCA
AATCGCGGCAATGTTCGATCTTAAATACGCTCATCCTTATACTTGGGCAGATCTCAATTCTGTTGTGAAACAGGCTTATA
GTCGTCGCAAGGCAACGTTAATTGAAATTAAAACGAATCCGAGTGATGGTAGCAGTTTGTATAAACGCTTAATCGAGCAA
ATTAGTCACGCCGTGATTGGTGCTTAA

Upstream 100 bases:

>100_bases
GGCAGGCTCGCAGCCATTGGAAGAATGGAAAGAAATTGAACGTAAAGCAGCAGGGTTAATTTCCTTGTTTGCAGAAGAAA
AATGATAACGGAGAAAAAAA

Downstream 100 bases:

>100_bases
GTATTCTGTAGGCGGGCTTTAGTCCGCCATATTTTATTATTTCTATCAATAATCCCTTCAATGATAAACATCATTTTTCT
TCACGGACTTCTTGGTACAA

Product: 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD [H]

Number of amino acids: Translated: 568; Mature: 567

Protein sequence:

>568_residues
MSVSVFNRCWSKVILETLVRQGVSHLCIAPGSRSTPLTLEAVRLQNAGAVTCHTHFDERGLGFFALGIAKATQSPVAIIV
TSGTATANLYPAIIEARQTGVNLFVLTADRPPELWECGANQAILQQNMFGQYPVANVNLPKPNADYSAQWLISLLEQAVF
QQKQQGGVVHINVPFAEPLYDATDEEVNSHSWLQPLQRWLIQNKSWINVEAQQNEVLMHENWDHWRTKRGVVVVGQLPAE
QAMGINSWASAMGWVLLTDIQSGVVPTTPYEDIWLANQTVREKLLQADIVIQFGARFISKRINQFLQAFKGEFWLVEQSG
KALDPYHHSLTRFNAKVHHWLRAHPPLRQKPWLLEPLALSKFCATFIEQQVGGNLTEASLALRLPTLLPYNGVLFLGNSL
LVRLVDALTQLPESYPVYTNRGASGIDGLLATAAGIGIGSNKPVVAVIGDTSTLYDLNSFALFKNVTQPTLIFVINNNGG
AIFDMLPVDEQVKDQFYRLPHNGDFSQIAAMFDLKYAHPYTWADLNSVVKQAYSRRKATLIEIKTNPSDGSSLYKRLIEQ
ISHAVIGA

Sequences:

>Translated_568_residues
MSVSVFNRCWSKVILETLVRQGVSHLCIAPGSRSTPLTLEAVRLQNAGAVTCHTHFDERGLGFFALGIAKATQSPVAIIV
TSGTATANLYPAIIEARQTGVNLFVLTADRPPELWECGANQAILQQNMFGQYPVANVNLPKPNADYSAQWLISLLEQAVF
QQKQQGGVVHINVPFAEPLYDATDEEVNSHSWLQPLQRWLIQNKSWINVEAQQNEVLMHENWDHWRTKRGVVVVGQLPAE
QAMGINSWASAMGWVLLTDIQSGVVPTTPYEDIWLANQTVREKLLQADIVIQFGARFISKRINQFLQAFKGEFWLVEQSG
KALDPYHHSLTRFNAKVHHWLRAHPPLRQKPWLLEPLALSKFCATFIEQQVGGNLTEASLALRLPTLLPYNGVLFLGNSL
LVRLVDALTQLPESYPVYTNRGASGIDGLLATAAGIGIGSNKPVVAVIGDTSTLYDLNSFALFKNVTQPTLIFVINNNGG
AIFDMLPVDEQVKDQFYRLPHNGDFSQIAAMFDLKYAHPYTWADLNSVVKQAYSRRKATLIEIKTNPSDGSSLYKRLIEQ
ISHAVIGA
>Mature_567_residues
SVSVFNRCWSKVILETLVRQGVSHLCIAPGSRSTPLTLEAVRLQNAGAVTCHTHFDERGLGFFALGIAKATQSPVAIIVT
SGTATANLYPAIIEARQTGVNLFVLTADRPPELWECGANQAILQQNMFGQYPVANVNLPKPNADYSAQWLISLLEQAVFQ
QKQQGGVVHINVPFAEPLYDATDEEVNSHSWLQPLQRWLIQNKSWINVEAQQNEVLMHENWDHWRTKRGVVVVGQLPAEQ
AMGINSWASAMGWVLLTDIQSGVVPTTPYEDIWLANQTVREKLLQADIVIQFGARFISKRINQFLQAFKGEFWLVEQSGK
ALDPYHHSLTRFNAKVHHWLRAHPPLRQKPWLLEPLALSKFCATFIEQQVGGNLTEASLALRLPTLLPYNGVLFLGNSLL
VRLVDALTQLPESYPVYTNRGASGIDGLLATAAGIGIGSNKPVVAVIGDTSTLYDLNSFALFKNVTQPTLIFVINNNGGA
IFDMLPVDEQVKDQFYRLPHNGDFSQIAAMFDLKYAHPYTWADLNSVVKQAYSRRKATLIEIKTNPSDGSSLYKRLIEQI
SHAVIGA

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788599, Length=566, Percent_Identity=47.7031802120141, Blast_Score=520, Evalue=1e-148,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.9 [H]

Molecular weight: Translated: 63098; Mature: 62967

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSVFNRCWSKVILETLVRQGVSHLCIAPGSRSTPLTLEAVRLQNAGAVTCHTHFDERG
CCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEEEECCCCEEEEEECCCCCC
LGFFALGIAKATQSPVAIIVTSGTATANLYPAIIEARQTGVNLFVLTADRPPELWECGAN
CCEEEEEHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHCCCC
QAILQQNMFGQYPVANVNLPKPNADYSAQWLISLLEQAVFQQKQQGGVVHINVPFAEPLY
HHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCC
DATDEEVNSHSWLQPLQRWLIQNKSWINVEAQQNEVLMHENWDHWRTKRGVVVVGQLPAE
CCCCHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCEEECCCCHHHHCCCCEEEEECCCHH
QAMGINSWASAMGWVLLTDIQSGVVPTTPYEDIWLANQTVREKLLQADIVIQFGARFISK
HHCCCHHHHHHHCCEEEEEHHCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHH
RINQFLQAFKGEFWLVEQSGKALDPYHHSLTRFNAKVHHWLRAHPPLRQKPWLLEPLALS
HHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
KFCATFIEQQVGGNLTEASLALRLPTLLPYNGVLFLGNSLLVRLVDALTQLPESYPVYTN
HHHHHHHHHHHCCCCCHHHHHEECCCCCCCCCEEEECHHHHHHHHHHHHHCCCCCCCEEC
RGASGIDGLLATAAGIGIGSNKPVVAVIGDTSTLYDLNSFALFKNVTQPTLIFVINNNGG
CCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCHHHCCHHHHHHHCCCCCEEEEEEECCCC
AIFDMLPVDEQVKDQFYRLPHNGDFSQIAAMFDLKYAHPYTWADLNSVVKQAYSRRKATL
EEEEECCCCHHHHHHHEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEE
IEIKTNPSDGSSLYKRLIEQISHAVIGA
EEEECCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SVSVFNRCWSKVILETLVRQGVSHLCIAPGSRSTPLTLEAVRLQNAGAVTCHTHFDERG
CCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEEEECCCCEEEEEECCCCCC
LGFFALGIAKATQSPVAIIVTSGTATANLYPAIIEARQTGVNLFVLTADRPPELWECGAN
CCEEEEEHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHCCCC
QAILQQNMFGQYPVANVNLPKPNADYSAQWLISLLEQAVFQQKQQGGVVHINVPFAEPLY
HHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCC
DATDEEVNSHSWLQPLQRWLIQNKSWINVEAQQNEVLMHENWDHWRTKRGVVVVGQLPAE
CCCCHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCEEECCCCHHHHCCCCEEEEECCCHH
QAMGINSWASAMGWVLLTDIQSGVVPTTPYEDIWLANQTVREKLLQADIVIQFGARFISK
HHCCCHHHHHHHCCEEEEEHHCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHH
RINQFLQAFKGEFWLVEQSGKALDPYHHSLTRFNAKVHHWLRAHPPLRQKPWLLEPLALS
HHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
KFCATFIEQQVGGNLTEASLALRLPTLLPYNGVLFLGNSLLVRLVDALTQLPESYPVYTN
HHHHHHHHHHHCCCCCHHHHHEECCCCCCCCCEEEECHHHHHHHHHHHHHCCCCCCCEEC
RGASGIDGLLATAAGIGIGSNKPVVAVIGDTSTLYDLNSFALFKNVTQPTLIFVINNNGG
CCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCHHHCCHHHHHHHCCCCCEEEEEEECCCC
AIFDMLPVDEQVKDQFYRLPHNGDFSQIAAMFDLKYAHPYTWADLNSVVKQAYSRRKATL
EEEEECCCCHHHHHHHEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEE
IEIKTNPSDGSSLYKRLIEQISHAVIGA
EEEECCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA