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The map label for this gene is dam

Identifier: 16272172

GI number: 16272172

Start: 223852

End: 224712

Strand: Direct

Name: dam

Synonym: HI0209

Alternate gene names: 16272172

Gene position: 223852-224712 (Clockwise)

Preceding gene: 16272171

Following gene: 30995360

Centisome position: 12.23

GC content: 35.0

Gene sequence:

>861_bases
ATGTTACGTCCGAAAAAACAATCTTTAAAACCTAAGTTAAAACACCGTCCATTTTTAAAATGGGCGGGGGGAAAATTTCG
TTTAACGGACGAGATAAATAAAGCCTTTCCAAACAAAAAAAACTGCTTAATTGAGCCATTTGTGGGCGCTGGAGCAGTAT
TTCTTAATTCTAATTTTGAACGCTATATTTTGGCAGATATCAATCCTGATTTAATCAATCTATTCAATATTGTGAAAGYA
AATGTAGATGGTTATATTGAAGATTGTAAGCCGATTTTCTTCGCTGATGATGCCAATACACCCGATTATTACTATGCTAA
ACGTCGCCAATTCAATGCATCAACAGAACCTTTCGAACGTTCGATTATTTTTTTGTATTTGAATCGTTTTGGTTTTAATG
GATTATGCCGTTATAACAGCAAGAATGAATTTAATGTACCTTTTGGTGCTTACAAAACTCATTATTTTCCAGAGGATGAA
TTACGTTATTTTGCGCATAAAGCGCAAAGTGCGGTGTTTTTATGCTGTGATTTTCAAAAAACTTTTGAATTTGCCGATAA
GGATTCGGTGATTTATTGTGATCCACCTTATGCTCCGCTACAACAAGAGACTAATTTTACTGGTTATGCGGGCAATGAGT
TTGGACTGGCGCAGCAACGTGCTTTGGCAGATTTAGCGAAATCTATACAAAAAGAAAAACAAATTTCGATATTGATCTCG
AATCACGATACTAAATTTACGCGTGAAATTTATAACGGTGCTAAATTTAAACGGGTAAAAGTCCAACGTTCTATTAGCCA
AAACCCAGAAAAACGCGTAAAAGTGAAAGAATTGATTGCGATATTTGGTGCTCGTAAATAA

Upstream 100 bases:

>100_bases
CTGGTATTACTCAAATCTCTAGGTCAAGCTTATGTGGCGAATGATACCGAACATACCCTCGTGTTAAACGCAATTCGTCG
TTGTACTCAAACGGATTAAG

Downstream 100 bases:

>100_bases
TAAAAATAACCCTTACTTTATACTAAAAGTACCAAAATCGTAATTTTAAAACCACAATAAAATAATATATTATGTATTAC
CATTTATCTCTTACAGTACC

Product: DNA adenine methylase

Products: NA

Alternate protein names: DNA adenine methyltransferase; Deoxyadenosyl-methyltransferase; M.HindIV

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MLRPKKQSLKPKLKHRPFLKWAGGKFRLTDEINKAFPNKKNCLIEPFVGAGAVFLNSNFERYILADINPDLINLFNIVKX
NVDGYIEDCKPIFFADDANTPDYYYAKRRQFNASTEPFERSIIFLYLNRFGFNGLCRYNSKNEFNVPFGAYKTHYFPEDE
LRYFAHKAQSAVFLCCDFQKTFEFADKDSVIYCDPPYAPLQQETNFTGYAGNEFGLAQQRALADLAKSIQKEKQISILIS
NHDTKFTREIYNGAKFKRVKVQRSISQNPEKRVKVKELIAIFGARK

Sequences:

>Translated_286_residues
MLRPKKQSLKPKLKHRPFLKWAGGKFRLTDEINKAFPNKKNCLIEPFVGAGAVFLNSNFERYILADINPDLINLFNIVKX
NVDGYIEDCKPIFFADDANTPDYYYAKRRQFNASTEPFERSIIFLYLNRFGFNGLCRYNSKNEFNVPFGAYKTHYFPEDE
LRYFAHKAQSAVFLCCDFQKTFEFADKDSVIYCDPPYAPLQQETNFTGYAGNEFGLAQQRALADLAKSIQKEKQISILIS
NHDTKFTREIYNGAKFKRVKVQRSISQNPEKRVKVKELIAIFGARK
>Mature_286_residues
MLRPKKQSLKPKLKHRPFLKWAGGKFRLTDEINKAFPNKKNCLIEPFVGAGAVFLNSNFERYILADINPDLINLFNIVKX
NVDGYIEDCKPIFFADDANTPDYYYAKRRQFNASTEPFERSIIFLYLNRFGFNGLCRYNSKNEFNVPFGAYKTHYFPEDE
LRYFAHKAQSAVFLCCDFQKTFEFADKDSVIYCDPPYAPLQQETNFTGYAGNEFGLAQQRALADLAKSIQKEKQISILIS
NHDTKFTREIYNGAKFKRVKVQRSISQNPEKRVKVKELIAIFGARK

Specific function: Methylates DNA within the sequence GATC. Directly involved in methyl-directed DNA mismatch repair

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family

Homologues:

Organism=Escherichia coli, GI1789789, Length=269, Percent_Identity=55.3903345724907, Blast_Score=302, Evalue=2e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DMA_HAEIN (P44431)

Other databases:

- EMBL:   L42023
- PIR:   H64054
- RefSeq:   NP_438378.1
- REBASE:   1152
- GeneID:   951117
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0209
- NMPDR:   fig|71421.1.peg.199
- TIGR:   HI_0209
- HOGENOM:   HBG586381
- OMA:   KARRTIS
- ProtClustDB:   CLSK870403
- BioCyc:   HINF71421:HI_0209-MONOMER
- BRENDA:   2.1.1.72
- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327
- Gene3D:   G3DSA:1.10.1020.10
- PRINTS:   PR00505
- TIGRFAMs:   TIGR00571

Pfam domain/function: PF02086 MethyltransfD12

EC number: =2.1.1.72

Molecular weight: Translated: 33165; Mature: 33165

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: PS00092 N6_MTASE

Important sites: BINDING 21-21 BINDING 25-25 BINDING 66-66 BINDING 194-194

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRPKKQSLKPKLKHRPFLKWAGGKFRLTDEINKAFPNKKNCLIEPFVGAGAVFLNSNFE
CCCCCCHHCCCHHHCCCCEEECCCEEEEHHHHHHHCCCCCCEEEECCCCCCEEEECCCCC
RYILADINPDLINLFNIVKXNVDGYIEDCKPIFFADDANTPDYYYAKRRQFNASTEPFER
EEEEECCCHHHHHHHHHHHHCCCCHHCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCHHH
SIIFLYLNRFGFNGLCRYNSKNEFNVPFGAYKTHYFPEDELRYFAHKAQSAVFLCCDFQK
EEEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCHH
TFEFADKDSVIYCDPPYAPLQQETNFTGYAGNEFGLAQQRALADLAKSIQKEKQISILIS
HHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEE
NHDTKFTREIYNGAKFKRVKVQRSISQNPEKRVKVKELIAIFGARK
CCCCHHHHHHHCCCCEEEHHHHHHHHCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLRPKKQSLKPKLKHRPFLKWAGGKFRLTDEINKAFPNKKNCLIEPFVGAGAVFLNSNFE
CCCCCCHHCCCHHHCCCCEEECCCEEEEHHHHHHHCCCCCCEEEECCCCCCEEEECCCCC
RYILADINPDLINLFNIVKXNVDGYIEDCKPIFFADDANTPDYYYAKRRQFNASTEPFER
EEEEECCCHHHHHHHHHHHHCCCCHHCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCHHH
SIIFLYLNRFGFNGLCRYNSKNEFNVPFGAYKTHYFPEDELRYFAHKAQSAVFLCCDFQK
EEEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCHH
TFEFADKDSVIYCDPPYAPLQQETNFTGYAGNEFGLAQQRALADLAKSIQKEKQISILIS
HHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEE
NHDTKFTREIYNGAKFKRVKVQRSISQNPEKRVKVKELIAIFGARK
CCCCHHHHHHHCCCCEEEHHHHHHHHCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800