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The map label for this gene is citF

Identifier: 16271997

GI number: 16271997

Start: 24267

End: 25769

Strand: Reverse

Name: citF

Synonym: HI0022

Alternate gene names: 16271997

Gene position: 25769-24267 (Counterclockwise)

Preceding gene: 30995348

Following gene: 16271996

Centisome position: 1.41

GC content: 41.25

Gene sequence:

>1503_bases
ATGACAACAAGAGAACAACGTATTGAAAAATATCACGCTGATCGCTCAGTCTATCAAGCGGTGCCTAAATCTGAATCCCT
TTCTCGTACAGCGAAAGATCGCAAACTTTGTACCTCTCTAGAAGAAGCGATTAAACGCTCTGGCTTAAAAGATGGTATGA
CTGTTTCATTTCATCATGCTTTTCGGGGCGGAGATTTTGTGGTGAATATGGTGATGAATAAAATTGCCGAAATGGGTTTC
AAAAATCTGACCCTTGCATCTTCATCTTTAATTGATAGCCACTTCCCGATTGTCGAACATATCAAAAATGGCGTGGTCAC
CAAAATCTATTCTTCTGGTTTACGCGGTGAACTTGCAGAGCAAATCTCTCGCGGTTTATTGAATGAACCTGTAAATATTC
ATTCTCATGGTGGACGTGTTCACTTAGTAAAATCTGGCGAATTAAAAATTGACGTTGCTTTTTTAGGCGTGCCTTGCTGT
GACACATTCGGTAATGCCAATGGATTTACTGGAAAAAGTAAATGCGGCTCTCTAGGCTATGCGCGTGTCGATGCTGAATA
TGCTGACAAAGTTGTGTTGCTCACAGAGGAATTTGTTGAATACCCGCACCACCCAATCAGCATAGCTCAAGATCAAGTAG
ATTTAATCGTGCAAGTTGAAGCAGTCGGTGACCCGAAAAAAATTGGTGGTGGTGCAACCCGAATGACTACCAATCCACGC
GAATTACTCATTGCGCGTAAATGTGCAGAAGTAATTTTTGCCAGTGGTTATTTTAAAGATGGCTTCTCATTACAAACTGG
TTCTGGGGGGGCAGCATTAGCTGTTACACGCTTTCTAGAAGAAAAAATGCGTCGTGAAAATATCACTGCAGATTTCGCAC
TTGGCGGAATTACTGCAAGTATGGTGGCATTACATGAGGCTGGTCTTATTAAAAAATTATTAGATGTACAAAGCTTTGAT
TCTGTTGCAGCTGAATCACTTGCCCGTAACCCAAATCATATTGAAGTCTCCGCTAACCAATATGCGAATTACAGTTCCAA
AGGCGCATCTGTTGAACGTTTAGATATGGTGATTCTTTCTGCTTTAGAAATCGACACTAAATTTAACGTGAATGTATTGA
CTGGCTCTGACGGTGTGATTCGCGGTGCATCTGGTGGTCATTGTGATACCGCAGCTTCTGCACAAGTAGCAATCATTGTG
GCACCATTAGTTCGTGGTCGTATTCCAACCGTGGTGGAAAACGTGATTACCTGTGTAACACCTGGTGAAAACGTCGATAT
TCTCGTTACCGATCATGGTGTAGCAGTAAATCCTAAACGTCCAGATTTGATTGAAGCTTTAAGCAAAACAGACATTCCAT
TGTTTACTATTGAACAACTTTGTGAACGTGCTTACAGCATTACTGGTAAACCAAAAGAAATTGAGTTTACTAATAAACCT
GTGGCTGTTGTTCGTTATCGTGATGGTTCGGTGATTGATACGGTGTATCAAGTTAAAGACTAG

Upstream 100 bases:

>100_bases
TAAACGGTAAAATGATTGACGCACCGATTATTGATCGCGCAAAACTTGTATTAGAACGTGCGAAATCAGGTATTCGTGAA
GAGTAAAGGGAGATAAAAAG

Downstream 100 bases:

>100_bases
AATCAGCATTACAAGCGATCTACTTTCAATAGCCAAAATTAACTCTTGTCTTCCCATGATTATCAGGAAAGGAGCCCAAA
GAGTTGAAAGGGACTCAATT

Product: citrate lyase subunit alpha

Products: NA

Alternate protein names: Citrase alpha chain; Citrate (pro-3S)-lyase alpha chain; Citrate CoA-transferase subunit

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MTTREQRIEKYHADRSVYQAVPKSESLSRTAKDRKLCTSLEEAIKRSGLKDGMTVSFHHAFRGGDFVVNMVMNKIAEMGF
KNLTLASSSLIDSHFPIVEHIKNGVVTKIYSSGLRGELAEQISRGLLNEPVNIHSHGGRVHLVKSGELKIDVAFLGVPCC
DTFGNANGFTGKSKCGSLGYARVDAEYADKVVLLTEEFVEYPHHPISIAQDQVDLIVQVEAVGDPKKIGGGATRMTTNPR
ELLIARKCAEVIFASGYFKDGFSLQTGSGGAALAVTRFLEEKMRRENITADFALGGITASMVALHEAGLIKKLLDVQSFD
SVAAESLARNPNHIEVSANQYANYSSKGASVERLDMVILSALEIDTKFNVNVLTGSDGVIRGASGGHCDTAASAQVAIIV
APLVRGRIPTVVENVITCVTPGENVDILVTDHGVAVNPKRPDLIEALSKTDIPLFTIEQLCERAYSITGKPKEIEFTNKP
VAVVRYRDGSVIDTVYQVKD

Sequences:

>Translated_500_residues
MTTREQRIEKYHADRSVYQAVPKSESLSRTAKDRKLCTSLEEAIKRSGLKDGMTVSFHHAFRGGDFVVNMVMNKIAEMGF
KNLTLASSSLIDSHFPIVEHIKNGVVTKIYSSGLRGELAEQISRGLLNEPVNIHSHGGRVHLVKSGELKIDVAFLGVPCC
DTFGNANGFTGKSKCGSLGYARVDAEYADKVVLLTEEFVEYPHHPISIAQDQVDLIVQVEAVGDPKKIGGGATRMTTNPR
ELLIARKCAEVIFASGYFKDGFSLQTGSGGAALAVTRFLEEKMRRENITADFALGGITASMVALHEAGLIKKLLDVQSFD
SVAAESLARNPNHIEVSANQYANYSSKGASVERLDMVILSALEIDTKFNVNVLTGSDGVIRGASGGHCDTAASAQVAIIV
APLVRGRIPTVVENVITCVTPGENVDILVTDHGVAVNPKRPDLIEALSKTDIPLFTIEQLCERAYSITGKPKEIEFTNKP
VAVVRYRDGSVIDTVYQVKD
>Mature_499_residues
TTREQRIEKYHADRSVYQAVPKSESLSRTAKDRKLCTSLEEAIKRSGLKDGMTVSFHHAFRGGDFVVNMVMNKIAEMGFK
NLTLASSSLIDSHFPIVEHIKNGVVTKIYSSGLRGELAEQISRGLLNEPVNIHSHGGRVHLVKSGELKIDVAFLGVPCCD
TFGNANGFTGKSKCGSLGYARVDAEYADKVVLLTEEFVEYPHHPISIAQDQVDLIVQVEAVGDPKKIGGGATRMTTNPRE
LLIARKCAEVIFASGYFKDGFSLQTGSGGAALAVTRFLEEKMRRENITADFALGGITASMVALHEAGLIKKLLDVQSFDS
VAAESLARNPNHIEVSANQYANYSSKGASVERLDMVILSALEIDTKFNVNVLTGSDGVIRGASGGHCDTAASAQVAIIVA
PLVRGRIPTVVENVITCVTPGENVDILVTDHGVAVNPKRPDLIEALSKTDIPLFTIEQLCERAYSITGKPKEIEFTNKPV
AVVRYRDGSVIDTVYQVKD

Specific function: Represents a citrate:acetyl-ACP transferase

COG id: COG3051

COG function: function code C; Citrate lyase, alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786832, Length=503, Percent_Identity=71.9681908548708, Blast_Score=756, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CILA_HAEIN (P44459)

Other databases:

- EMBL:   L42023
- PIR:   C64043
- RefSeq:   NP_438195.1
- ProteinModelPortal:   P44459
- SMR:   P44459
- GeneID:   950920
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0022
- NMPDR:   fig|71421.1.peg.21
- TIGR:   HI_0022
- HOGENOM:   HBG297040
- OMA:   TGLKDGM
- ProtClustDB:   CLSK874113
- BioCyc:   HINF71421:HI_0022-MONOMER
- BRENDA:   2.8.3.10
- BRENDA:   4.1.3.6
- InterPro:   IPR006472
- PIRSF:   PIRSF009451
- TIGRFAMs:   TIGR01584

Pfam domain/function: PF04223 CitF

EC number: =4.1.3.6; =2.8.3.10

Molecular weight: Translated: 54134; Mature: 54003

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTREQRIEKYHADRSVYQAVPKSESLSRTAKDRKLCTSLEEAIKRSGLKDGMTVSFHHA
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEC
FRGGDFVVNMVMNKIAEMGFKNLTLASSSLIDSHFPIVEHIKNGVVTKIYSSGLRGELAE
CCCCHHHHHHHHHHHHHCCCCCEEECHHHHHHCCCHHHHHHHCCHHHHHHHCCCCHHHHH
QISRGLLNEPVNIHSHGGRVHLVKSGELKIDVAFLGVPCCDTFGNANGFTGKSKCGSLGY
HHHHHHHCCCCEEECCCCEEEEEECCCEEEEEEEECCCCHHCCCCCCCCCCCCCCCCCCE
ARVDAEYADKVVLLTEEFVEYPHHPISIAQDQVDLIVQVEAVGDPKKIGGGATRMTTNPR
EEECHHHHCEEEEEEHHHHHCCCCCCCCCCCCEEEEEEEEECCCHHHCCCCCCEECCCHH
ELLIARKCAEVIFASGYFKDGFSLQTGSGGAALAVTRFLEEKMRRENITADFALGGITAS
HHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCHHEEHHHHHHH
MVALHEAGLIKKLLDVQSFDSVAAESLARNPNHIEVSANQYANYSSKGASVERLDMVILS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHCCCCCCCCCCHHHHHHHHHH
ALEIDTKFNVNVLTGSDGVIRGASGGHCDTAASAQVAIIVAPLVRGRIPTVVENVITCVT
HHEECCEEEEEEEECCCCEEECCCCCCCCCCCCCEEEEEEEHHHCCCCHHHHHHHEEEEC
PGENVDILVTDHGVAVNPKRPDLIEALSKTDIPLFTIEQLCERAYSITGKPKEIEFTNKP
CCCCEEEEEECCCEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEECCCC
VAVVRYRDGSVIDTVYQVKD
EEEEEECCCCEEEHHEECCC
>Mature Secondary Structure 
TTREQRIEKYHADRSVYQAVPKSESLSRTAKDRKLCTSLEEAIKRSGLKDGMTVSFHHA
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEC
FRGGDFVVNMVMNKIAEMGFKNLTLASSSLIDSHFPIVEHIKNGVVTKIYSSGLRGELAE
CCCCHHHHHHHHHHHHHCCCCCEEECHHHHHHCCCHHHHHHHCCHHHHHHHCCCCHHHHH
QISRGLLNEPVNIHSHGGRVHLVKSGELKIDVAFLGVPCCDTFGNANGFTGKSKCGSLGY
HHHHHHHCCCCEEECCCCEEEEEECCCEEEEEEEECCCCHHCCCCCCCCCCCCCCCCCCE
ARVDAEYADKVVLLTEEFVEYPHHPISIAQDQVDLIVQVEAVGDPKKIGGGATRMTTNPR
EEECHHHHCEEEEEEHHHHHCCCCCCCCCCCCEEEEEEEEECCCHHHCCCCCCEECCCHH
ELLIARKCAEVIFASGYFKDGFSLQTGSGGAALAVTRFLEEKMRRENITADFALGGITAS
HHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCHHEEHHHHHHH
MVALHEAGLIKKLLDVQSFDSVAAESLARNPNHIEVSANQYANYSSKGASVERLDMVILS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHCCCCCCCCCCHHHHHHHHHH
ALEIDTKFNVNVLTGSDGVIRGASGGHCDTAASAQVAIIVAPLVRGRIPTVVENVITCVT
HHEECCEEEEEEEECCCCEEECCCCCCCCCCCCCEEEEEEEHHHCCCCHHHHHHHEEEEC
PGENVDILVTDHGVAVNPKRPDLIEALSKTDIPLFTIEQLCERAYSITGKPKEIEFTNKP
CCCCEEEEEECCCEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEECCCC
VAVVRYRDGSVIDTVYQVKD
EEEEEECCCCEEEHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800